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Maize Silage (maize + silage)
Selected AbstractsDegradation of transgenic Cry1Ab DNA and protein in Bt-176 maize during the ensiling processJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2006B. Lutz Summary Maize silage is commonly used as feed for farm animals. The aim of this study was to monitor the time-dependent degradation of non-recombinant chloroplast DNA (exemplified by the rubisco gene) in comparison with the recombinant cry1Ab gene in the course of the ensiling process. In parallel, the Cry1Ab protein content and fragment sizes were determined. Fragments of the rubisco (173, 896, 1197, 1753 and 2521 bp) and of the cry1Ab gene (211, 420, 727 and 1423 bp) were selected to investigate the DNA degradation process. The detection of the Cry1Ab protein was performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Rubisco gene fragments of 173 bp were still detectable after 61 days, while fragments of 1197 and 2521 bp were detectable up to 30 days and on the first day only respectively. Polymerase chain reaction (PCR) analyses revealed that fragments of the cry1Ab gene with sizes of 211 and 420 bp were detectable up to 61 days, fragments with sizes of 727 and 1423 bp, 30 and 6 days respectively. The ELISA showed a decrease of the Cry1Ab protein in maize silage during the ensiling process. No marked degradation was observed during the first 43 h. Thereafter, a sharp decrease was measured. After 61 days, 23.6 ± 0.9% of the initial Cry1Ab protein was still detectable. Immunoblotting confirmed the results of the ELISA showing a positive signal of approximately 60 kDa size for 8 days of ensiling; no further immunoactive fragments were detectable by immunoblotting. In conclusion, the ensiling process markedly decreases the presence of long functional cry1Ab gene fragments and full size Cry1Ab protein. [source] Effects of different rates and timing of application of nitrogen as slurry and mineral fertilizer on yield of herbage and nitrate-leaching potential of a maize/Italian ryegrass cropping system in north-west PortugalGRASS & FORAGE SCIENCE, Issue 1 2009H. Trindade Abstract Efficient use of cattle-slurry to avoid nitrogen (N) leaching and other losses is important in designing intensive dairy systems to minimize pollution of air and water. The response in dry-matter (DM) yield of herbage and nitrate-leaching potential to different rates and timing of application of N as cattle slurry and/or mineral fertilizer in a double-cropping system producing maize (Zea mays L.) silage and Italian ryegrass (Lolium multiflorum Lam.) was investigated in north-west Portugal. Nine treatments with different rates and combinations of cattle slurry, and with or without mineral-N fertilizer, applied at sowing and as a top-dressing to both crops, were tested and measurements were made of DM yield of herbage, N concentration of herbage, uptake of N by herbage and amounts of residual soil nitrate-N to a depth of 1 m, in a 3-year experiment. Regression analysis showed that the application of 150 and 100 kg of available N ha,1 to maize and Italian ryegrass, respectively, resulted in 0·95 of maximum DM yields of herbage and 0·90 of maximum N uptake by herbage. Residual amounts of nitrate-N in soil after maize ranged from 48 to 278 kg N ha,1 with an exponential increase in response to the amount of N applied; there were higher values of nitrate-leaching potential when mineral-N fertilizer was applied. The results suggest that it is possible in highly productive maize/Italian ryegrass systems to obtain high DM yields of herbage for maize silage and Italian ryegrass herbage with minimal leaching losses by using slurry exclusively at annual rates of up to 250 kg available N ha,1 (equivalent to 480 kg total N ha,1) in three applications. [source] Production and behavioural responses of high- and low-yielding dairy cows to different periods of access to grazing or to a maize silage and soyabean meal diet fed indoorsGRASS & FORAGE SCIENCE, Issue 4 2006O. Hernandez-Mendo Abstract The study examined whether high-yielding cows grazing pasture respond differently from low-yielding cows in milk production and feeding behaviour, to increasing the time made available for eating a maize silage and soyabean meal (TAMS) diet indoors and reducing the time available for grazing (TAG). Two experiments, each lasting 42 d, were carried out in spring (Experiment 1) and autumn (Experiment 2) using Holstein-Friesian cows at two different levels of milk yield (MYL). Milk production and feeding behaviour were examined for TAG + TAMS systems of 19 h, TAG plus 1 h TAMS (19 + 1), and 5 h TAG plus 15 h TAMS (5 + 15). There were two levels of concentrate (0 and 6 kg d,1), and in the spring experiment two sward heights (4,6 and 8,10 cm) were also studied. Milk yield, persistency of milk yield, liveweight change and estimated total DM intake were significantly higher on the 5 + 15 than on the 19 + 1 grazing system in Experiment 1 but not in Experiment 2. There were no significant interactions of TAG + TAMS treatment with MYL for any production or behavioural measurements except for maize silage feeding time, where high MYL cows spent a significantly greater time eating maize silage than low MYL cows on the 5 + 15 treatment but not on the 19 + 1 treatment. It can be concluded that high- and low-yielding cows respond similarly in milk production and feeding behaviour to different combinations of TAG and TAMS. In autumn, estimated daily intakes of herbage were lower on both grazing treatments relative to spring, resulting from lower rates of herbage intake with no compensatory increase in grazing time. In contrast, rates of intake of maize silage were higher in autumn especially on the 19 + 1 system. These results may imply a change of preference from herbage to maize silage between spring and autumn. [source] Degradation of transgenic Cry1Ab DNA and protein in Bt-176 maize during the ensiling processJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2006B. Lutz Summary Maize silage is commonly used as feed for farm animals. The aim of this study was to monitor the time-dependent degradation of non-recombinant chloroplast DNA (exemplified by the rubisco gene) in comparison with the recombinant cry1Ab gene in the course of the ensiling process. In parallel, the Cry1Ab protein content and fragment sizes were determined. Fragments of the rubisco (173, 896, 1197, 1753 and 2521 bp) and of the cry1Ab gene (211, 420, 727 and 1423 bp) were selected to investigate the DNA degradation process. The detection of the Cry1Ab protein was performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Rubisco gene fragments of 173 bp were still detectable after 61 days, while fragments of 1197 and 2521 bp were detectable up to 30 days and on the first day only respectively. Polymerase chain reaction (PCR) analyses revealed that fragments of the cry1Ab gene with sizes of 211 and 420 bp were detectable up to 61 days, fragments with sizes of 727 and 1423 bp, 30 and 6 days respectively. The ELISA showed a decrease of the Cry1Ab protein in maize silage during the ensiling process. No marked degradation was observed during the first 43 h. Thereafter, a sharp decrease was measured. After 61 days, 23.6 ± 0.9% of the initial Cry1Ab protein was still detectable. Immunoblotting confirmed the results of the ELISA showing a positive signal of approximately 60 kDa size for 8 days of ensiling; no further immunoactive fragments were detectable by immunoblotting. In conclusion, the ensiling process markedly decreases the presence of long functional cry1Ab gene fragments and full size Cry1Ab protein. [source] Selenium supplementation and selenium status of dairy cows fed diets based on grass, grass silage or maize silageJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2002M. GIERUS In three separate trial series (TS) the effect of diet composition on selenium (Se) status of dairy cows were investigated. Diets were formulated based mainly on grass (TS1), grass silage (TS2) or maize silage (TS3) with different levels of Se supplementation. Each TS comprised a total of 30 dairy cows and contained one treatment group without Se supplementation (control) and two groups with increasing levels of Se supplementation (levels 1 and 2). Selenium was administered as Na-selenite. The control groups of the different TS showed a very low Se supply of 38,54 ,g Se/kg DM. At level 1 the Se supply was increased to 102,165 ,g Se/kg DM and at level 2 was 294,373 ,g Se/kg DM. After completion of the 6-week trials the average plasma Se concentration of the control cows (without Se supplementation) across all TS was 21.5 ,g/l; this increased significantly following Se supplementation, to 37.7 ,g/l at level 1 and 61.5 ,g/l at level 2. The plasma glutathione peroxidase (GSH-Px) activity of the control cows averaged 67 U/l, rising considerably after supplementation at level 1 to a value of 101 U/l, but showed little further increase at level 2 with a mean value of 120 U/l. By contrast, the average Se content of the milk was unchanged in the control and level 1 groups at 10.5 ,g/kg and 10.9 ,g/kg, respectively, and only increased markedly after supplementation at level 2 to a mean value of 15.1 ,g/kg. The diet based on maize silage, while having a similar Se content as the grass and grass silage-based diets, resulted in a slightly improved Se status, which is due to a higher Se intake from soybean meal. [source] Seasonal changes of CLA isomers and other fatty acids of milk fat from grazing dairy herds in the AzoresJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 10 2008Oldemiro A Rego Abstract BACKGROUND: Season of the year associated with dietary changes has been recognized as a factor implicated in milk fat fatty acid (FA) profile in dairy cows. However, a lack of information exists concerning cows grazing all year round as is practiced in the Azores, where cows are supplemented in winter with maize silage plus concentrates, while in spring the higher grass allowance only requires supplementation with concentrate. The main objective of this study was to detect any seasonal variation of FA profile of milk fat from milk sampled in bulk tanks of 12 Azorean dairy herds. RESULTS Compared to winter milk, milk fat from spring presented a higher proportion of CLA cis -9,trans -11 (14.3 versus 9.6 g kg,1 FA), C18:1 trans -11 (32 versus 22 g kg,1 FA), C18:2 trans -11,cis -15 (3.7 versus 2.2 g kg,1 FA), CLA trans -11,cis -13 (0.34 versus 0.23 g kg,1 FA) and C18:3 n-3 (5.7 versus 5.4 g kg,1 FA). The C18:2 n-6/C18:3 n-3 ratio was lower (P < 0.05) in spring. Branched-chain FA, except the anteiso -C15:0, were higher in spring, while odd-chain FA (C15:0) were higher in winter. CONCLUSION: Dairy herd management in the Azores presents a seasonal variation of milk fat FA composition, where the spring milk may present increased potential benefits for human consumers. Copyright © 2008 Society of Chemical Industry [source] Comparison of sheep and red deer rumen fluids for assessing nutritive value of ruminant feedstuffsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 14 2005Gonzalo Hervás Abstract Four forages (alfalfa hay, barley straw, maize silage and beet pulp), four cereal grains (barley, maize, sorghum and wheat) and four shrubs (Calluna vulgaris, Erica australis, Cytisus cantabricus and Genista occidentalis) were incubated using rumen fluids from sheep and red deer, to examine differences in in vitro fermentation rates and ruminal parameters. For the forages and cereal grains, results suggest few differences between species in parameters related to gas production, rates of fermentation, organic matter disappearance and extent of degradation. Deer showed slightly better results for shrubs than sheep did. Disappearance of neutral detergent fibre was generally greater when the rumen fluid was derived from red deer (P < 0.05). On the other hand, ammonia-N concentration and total volatile fatty acids (VFAs) were higher in sheep (P < 0.05). Molar proportions of the major VFAs showed significant differences (P < 0.05) associated with the species of the inoculum donor and suggest that fermentation pathways might have been more efficient in red deer. The estimated amount of methane was higher in sheep, regardless of the substrate incubated (P < 0.001). The results indicate that although the sheep could be valid as a model to assess the nutritive value of good quality feedstuffs for red deer, it would fail to offer reliable information on non-conventional, low-quality feeds such as shrubs. Copyright © 2005 Society of Chemical Industry [source] Effects of maize (Zea mays L.) silage feeding on dry matter intake and milk production of dairy buffalo and cattle in Tarai, NepalANIMAL SCIENCE JOURNAL, Issue 4 2009Yoshiaki HAYASHI ABSTRACT To identify the effects of whole crop maize silage (MS) as a substitute for rice straw (RS) on feed intake and milk production of mid-late lactating buffalo and cattle in Tarai, Nepal, eight Murrah and eight Jersey-Hariana were fed the basal diet, RS (ad libitum) with concentrate (0.68% of bodyweight [BW] on a dry matter [DM] basis). A 4 × 4 Latin square design experiment was conducted in each animal species with graded levels of MS substitution for RS (0%, T1; 33%, T2; 67%, T3 and 100%, T4). The MS had higher digestibility and total digestible nutrient (TDN) than RS. The DM intake per BW of the both species was highest in T3. The substitution of MS for RS increased the crude protein intake and the TDN intake in the both species. Although the buffalo showed the highest milking performance in T4, the cattle showed no significant differences in their milking performance among the treatments. The substitution of MS for RS improved the feed intake and milk production in the buffalo. On the other hand, the milk yield was not raised in the cattle, though the feed intake was increased by the substitution. [source] Starch and fibre distribution in maize silages in relation to particle sizeJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 3 2003I Fernandez Abstract In a first trial the particle size distribution of four maize silages was measured by two different sieving methods, dry and wet sieving. Particle size distribution was quantified by three values, mean particle size, extent of dispersion, and proportion of large particles. Wet sieving separated particles better than dry sieving and was more suitable for measuring maize silage particle size. In a second trial the particle size distribution of 10 maize hybrids grown in the same conditions was measured by wet sieving. Starch proportion in large particles varied widely between maize hybrids, from 39.7 to 82.4%, against a variation from 75.4 to 100% for cell wall proportion in the same class of particles, and these variations might be predicted by dry matter distribution. © 2003 Society of Chemical Industry [source] | ||||||||||