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Magnetic Particles (magnetic + particle)

Distribution by Scientific Domains

Life Sciences	42%
Polymers and Materials Science	30%
Chemistry	18%

Selected Abstracts

PURIFICATION OF AMYLASE FROM TILAPIA BY MAGNETIC PARTICLE

JOURNAL OF FOOD PROCESSING AND PRESERVATION, Issue 1 2010
MING CHANG WU
ABSTRACT Recent development in magnetic carrier technology involves the use of nonmagnetic substrates attached to superparamagnetic particles forming functionally modified magnetic support to isolate a particular enzyme in a controllable magnetic field. In this study, the superparamagnetic particles were modified by epichlorohydrin and other agents to cross-link with starch to form the purification support. This affinity support was able to bind the amylase and was used in the purification of amylase from Taiwan tilapia. After ammonium sulfate precipitation of amylase from Taiwan tilapia, the modified superparamagnetic particles were able to purify the crude amylase by 20.78-fold with recovery of activity of 75.6%. The molecular weight of the amylase was estimated to be 66.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both crude and purified amylase reached an optimum at a pH of 8.0 and temperature of 50C, and the enzyme was stable between 20 and 50C. PRACTICAL APPLICATIONS Because of the rapid development of high technology such as carrier supports for enzyme purification, the development, research and application of magnetic carriers are timely needed. The present study demonstrated that the affinity superparamagnetic particles could be used as a carrier support to absorb and purify the amylase and that technology of affinity purification can be widely used in protein purification. Compared with the traditional chromatography used in the purification of proteins, this novel affinity superparamagnetic particle technology is rapid, has low operation cost, requires simple facilities, and involves easy separation and recovery of the enzymes. [source]

Cover Picture: Programmable Motion and Separation of Single Magnetic Particles on Patterned Magnetic Surfaces (Adv. Mater.

ADVANCED MATERIALS, Issue 14 2005
14/2005)
Abstract Structured magnetic surfaces enabling programmable motion of single micrometer-sized magnetic particles are reported on p.,1730 by Gunnarsson and co-workers. Patterns of thin-film magnetic elements are tailored to form transport lines with junctions for the separation of individual particles. This method has the potential to improve and generate new applications in biotechnology. The cover shows a schematic of the transportation and separation of magnetic particles functionalized with antibodies capable of selectively capturing the corresponding analytes from a sample. [source]

Poly(ethylene glycol) Surface Coated Magnetic Particles

MACROMOLECULAR RAPID COMMUNICATIONS, Issue 18 2005
Christophe Flesch
Abstract Summary: A methacrylate-functionalized poly(ethylene glycol) macromonomer was copolymerized at the surface of methacrylate-derivatized maghemite nanoparticles. After silylation of the magnetic core with methacryloxypropyltrimethoxysilane, two grafting procedures based on either a direct copolymerization reaction in water or an inverse emulsion polymerization were compared. A direct copolymerization led to low polymer surface amounts, whereas an inverse emulsion process allowed nanocomposite particles containing up to 90 wt.-% polymer to be obtained. TEM picture of maghemite-PEG hybrid particles. [source]

A Microchemical System with Continuous Recovery and Recirculation of Catalyst-Immobilized Magnetic Particles,

ANGEWANDTE CHEMIE, Issue 38 2010
Dr. Chan, Pil Park
Alles im Fluss: Ein mikromechanisches System für katalytische Durchflussreaktionen mit einem magnetischen Katalysator wird vorgestellt (siehe Bild). Der Mikroreaktor ermöglicht die automatische Abtrennung und Wiederzuführung von Katalysatorpartikeln und ist auf verschiedenste katalytische Reaktionen anwendbar. [source]

Control of Drug Release through the In Situ Assembly of Stimuli-Responsive Ordered Mesoporous Silica with Magnetic Particles

CHEMPHYSCHEM, Issue 17 2007
Shenmin Zhu Dr.
Abstract A site-selective controlled delivery system for controlled drug release is fabricated through the in situ assembly of stimuli-responsive ordered SBA-15 and magnetic particles. This approach is based on the formation of ordered mesoporous silica with magnetic particles formed from Fe(CO)5 via the surfactant-template sol-gel method and control of transport through polymerization of N-isopropyl acrylamide inside the pores. Hydrophobic Fe(CO)5 acts as a swelling agent as well as being the source of the magnetic particles. The obtained system demonstrates a high pore diameter (7.1 nm) and pore volume (0.41 cm3,g,1), which improves drug storage for relatively large molecules. Controlled drug release through the porous network is demonstrated by measuring the uptake and release of ibuprofen (IBU). The delivery system displays a high IBU storage capacity of 71.5 wt,%, which is almost twice as large as the highest value based on SBA-15 ever reported. In vitro testing of IBU loading and release exhibits a pronounced transition at around 32,°C, indicating a typical thermosensitive controlled release. [source]

Magnetic investigation of heavy metals contamination in urban topsoils around the East Lake, Wuhan, China

GEOPHYSICAL JOURNAL INTERNATIONAL, Issue 2 2007
Tao Yang
SUMMARY Magnetic measurements and heavy metal analyses were performed on 133 samples from the urban soils around the East Lake in Wuhan, China. Samples were collected from four areas with different environmental settings: a heavy industrial area well known for thermal power generation and steel works; villages located in the downwind area of the industrial area; a main road with heavy traffic and roads around the East Lake. Results show that concentrations of magnetic particle and heavy metals in urban topsoils are significantly elevated due to the input of coarser-grained magnetite from industrial (e.g. power generation and steel production) and other anthropogenic activities (e.g. vehicle emissions). Concentration-related magnetic parameters, for example, magnetic susceptibility, saturation isothermal remanent magnetization and anhysteretic remanent magnetization, significantly correlate with the concentration of heavy metals. Moreover, in terms of grain sizes, the magnetic particles of different origins can be efficiently discriminated at the studied region. Therefore, magnetic measurements provide a basis for discrimination and identification of different contamination sources, and can be used as an economic alternative to chemical analysis when mapping heavy metal contamination in urban soil around the East Lake region, Wuhan, China. [source]

Effect of a gas,liquid separator on the hydrodynamics and circulation flow regimes in internal-loop airlift reactors

JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 5 2001
Jaroslav Klein
Abstract The role of the gas,liquid separator on hydrodynamic characteristics in an internal-loop airlift reactor (ALR) was investigated. Both gas holdup and liquid velocity were measured in a 30,dm3 airlift reactor with two different head configurations: with and without an enlarged separator. A magnetic tracer method using a neutrally buoyant magnetic particle as flowfollower was used to measure the liquid velocity in all sections of the internal-loop airlift reactor. Average liquid circulation velocities in the main parts of the ALR were compared for both reactor configurations. At low air flow rates the separator had no influence on gas holdup, circulation velocity and intensity of turbulence in the downcomer and separator. At higher superficial air velocities, however, the separator design had a decisive effect on the hydrodynamic parameters in the downcomer and the separator. On the other hand, the gas holdup in the riser was only slightly influenced by the separator configuration in the whole range of air flow. Circulation flow regimes, characterising the behaviour of bubbles in the downcomer, were identified and the effect of the separator on these regimes was assessed. © 2001 Society of Chemical Industry [source]

Synthesis of Novel Porous Magnetic Silica Microspheres as Adsorbents for Isolation of Genomic DNA

BIOTECHNOLOGY PROGRESS, Issue 2 2006
Zhichao Zhang
An improved procedure is described for preparation of novel mesoporous microspheres consisting of magnetic nanoparticles homogeneously dispersed in a silica matrix. The method is based on a three-step process, involving (i) formation of hematite/silica composite microspheres by urea-formaldehyde polymerization, (ii) calcination of the composite particles to remove the organic constituents, and (iii) in situ transformation of the iron oxide in the composites by hydrogen reductive reaction. The as-synthesized magnetite/silica composite microspheres were nearly monodisperse, mesoporous, and magnetizable, with as typical values an average diameter of 3.5 ,m, a surface area of 250 m2/g, a pore size of 6.03 nm, and a saturation magnetization of 9.82 emu/g. These magnetic particles were tested as adsorbents for isolation of genomic DNA from Saccharomyces cerevisiae cells and maize kernels. The results are quite encouraging as the magnetic particle based protocols lead to the extraction of genomic DNA with satisfactory integrity, yield, and purity. Being hydrophilic in nature, the porous magnetic silica microspheres are considered a good alternative to polystyrene-based magnetic particles for use in biomedical applications where nonspecific adsorption of biomolecules is to be minimized. [source]

Detection of C Reactive Protein (CRP) in Serum by an Electrochemical Aptamer-Based Sandwich Assay

ELECTROANALYSIS, Issue 11 2009
Sonia Centi
Abstract A disposable electrochemical assay involving magnetic particles and carbon-based screen-printed electrodes (SPCEs) was developed for the detection of C Reactive Protein (CRP). CRP is a plasma protein and is among the most expressed proteins in acute phase inflammation cases, being a known biomarker for inflammatory states. The assay was based on a sandwich format in which a RNA aptamer was coupled to a monoclonal antibody and alkaline phosphatase (AP) was used as enzymatic label. After the sandwich assay, the modified magnetic beads were captured by a magnet on the surface of a graphite working electrode and the electrochemical detection was thus achieved through the addition of the AP substrate (,-naphthyl-phosphate) and ,-naphthol produced during the enzymatic reaction was detected using differential pulse voltammetry (DPV). The parameters influencing the different steps of the assay were optimized in order to reach the best sensitivity and specificity. With the optimized conditions, the assay was applied to the analysis of CRP free serum and serum samples. [source]

On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles

ELECTROPHORESIS, Issue 24 2008
Anne Le Nel
Abstract As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles. [source]

Grazing protozoa and magnetosome dissolution in magnetotactic bacteria

ENVIRONMENTAL MICROBIOLOGY, Issue 11 2007
Juliana L. Martins
Summary Magnetotactic bacteria show an ability to navigate along magnetic field lines because of magnetic particles called magnetosomes. All magnetotactic bacteria are unicellular except for the multicellular prokaryote (recently named ,Candidatus Magnetoglobus multicellularis'), which is formed by an orderly assemblage of 17,40 prokaryotic cells that swim as a unit. A ciliate was used in grazing experiments with the M. multicellularis to study the fate of the magnetosomes after ingestion by the protozoa. Ciliates ingested M. multicellularis, which were located in acid vacuoles as demonstrated by confocal laser scanning microscopy. Transmission electron microscopy and X-ray microanalysis of thin-sectioned ciliates showed the presence of M. multicellularis and magnetosomes inside vacuoles in different degrees of degradation. The magnetosomes are dissolved within the acidic vacuoles of the ciliate. Depending on the rate of M. multicellularis consumption by the ciliates the iron from the magnetosomes may be recycled to the environment in a more soluble form. [source]

Field-Structured Chemiresistors: Field-Structured Chemiresistors (Adv. Funct.

ADVANCED FUNCTIONAL MATERIALS, Issue 10 2010
Mater.
D. H. Read and J. E. Martin report on page 1577 the use of field-structured composites as improved chemiresistors. Magnetic fields are used to organize Au-coated magnetic particles into electrically conducting pathways within a polymer matrix. Though many structures are possible, a biaxial field leads to stacked particle sheets, as illustrated on the cover. These sensors undergo a reversible conductor,insulator transition over such a narrow concentration range that they behave like chemical switches. This switching point is tuned by controlling polymer stresses. [source]

Repeated Transfer of Colloidal Patterns by Using Reversible Buckling Process

ADVANCED FUNCTIONAL MATERIALS, Issue 13 2009
Dong Choon Hyun
Abstract The reversible nature of buckling is employed to repeatedly transfer colloids assembled in buckling patterns to flat surfaces. The cycle of colloidal loading,transfer,buckling is repeatedly carried out to fabricate the same colloidal patterns. The key to success is the reduction in the amplitude of the buckling patterns to a few nanometers as well as the recovery of initial buckling patterns after repeated stretching. The reduced buckling amplitude by poststretching or thermal annealing embosses the colloids assembled in the trenches of the buckling patterns, which enables the transfer regardless of the size, species, or layer thickness of the particles. This report demonstrates various transferred patterns composed of colloidal crystals, fluorescence hydrogel colloids, Au nanoparticles, and iron oxide magnetic particles. Since the process does not require surface modification of the colloids, it can be used to fabricate any colloidal patterns. [source]

Magnetic investigation of heavy metals contamination in urban topsoils around the East Lake, Wuhan, China

First-order reversal curve diagrams and thermal relaxation effects in magnetic particles

GEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2001
Christopher R. Pike
Summary We have recently developed a technique for characterizing the magnetic components within natural particle assemblages. This technique is based on the transformation of magnetization data from first-order reversal curves (FORCs) into contour plots of a 2-D distribution function (FORC diagrams). FORC diagrams are useful for obtaining information about switching fields and interactions in magnetic particle systems. Here, we examine experimental data and a theoretical model in order to provide a rigorous framework for interpreting FORC diagrams for samples that contain superparamagnetic particles. We have found four distinct manifestations of thermal relaxation on FORC diagrams. First, thermal relaxation will shift the FORC distribution to lower coercivities. Second, at intermediate temperatures, thermal relaxation can generate a secondary peak about the origin of a FORC diagram. This secondary peak indicates that part of a single-domain particle assemblage has become superparamagnetic. At high enough temperatures, the primary peak of the FORC distribution will be located about the origin of a FORC diagram. Third, thermal relaxation can produce a small, but systematic, upward shift of a FORC distribution. Fourth, thermal relaxation will produce contours that lie near and parallel to the vertical axis in the lower quadrant of a FORC diagram. These manifestations make FORC diagrams a powerful tool for studying the effects of thermal relaxation (superparamagnetism) in bulk natural samples, particularly when the samples contain mixed magnetic particle assemblages. [source]

Magnetically Triggered Reversible Controlled Drug Delivery from Microfabricated Polymeric Multireservoir Devices

ADVANCED MATERIALS, Issue 40 2009
Kaiyong Cai
The pulsed reversible release of dual drugs from biodegradable polymeric multireservoir devices is successfully demonstrated. The controlled release is achieved by incorporating magnetic particles in the devices as switch carriers. It is possible to intentionally switch on/off the drug release at any desired time for a chosen duration. [source]

Microfluidic Assembly of Magnetic Hydrogel Particles with Uniformly Anisotropic Structure

ADVANCED MATERIALS, Issue 31 2009
Chia-Hung Chen
Monodisperse magnetic particles are templated from double emulsions formed using sequential flow-focus drop formation. The microfluidic drop formation allows the particles to be formed with high monodispersity and with consistently anisotropic internal structure. This structural anisotropy gives rise to magnetic anisotropy, allowing the particles to be rotated by a magnetic field. [source]

Cover Picture: Programmable Motion and Separation of Single Magnetic Particles on Patterned Magnetic Surfaces (Adv. Mater.

Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum

MOLECULAR ECOLOGY RESOURCES, Issue 4 2004
THORSTEN B. H. REUSCH
Abstract We present primers for five polymorphic microsatellite loci in the eye fluke Diplostomum pseudospathaceum (Trematoda), a widely distributed parasite with a complex life cycle used as a model for parasitology and fish immunology. The loci were identified using a GA/CT-enriched genomic library by subtractive hybridization with magnetic particles. All five loci were highly polymorphic, displaying 17 to 61 alleles and heterozygosities ranging from 0.53 to 0.92. We isolated populations of parasites within the first (snail) and second (fish) intermediate host and found small but significant genetic differentiation (FST = 0.012) between the two life stages of the parasite. [source]

Isolation of dinucleotide microsatellite loci from red-backed salamander (Plethodon cinereus)

MOLECULAR ECOLOGY RESOURCES, Issue 1 2003
Lisa M. Connors
Abstract We isolated 13 variable dinucleotide microsatellites from red-backed salamanders (Plethodon cinereus). After generating fragments using degenerate oligonucleotide primer-polymerase chain reaction (DOP-PCR), AC repeats were captured using biotinylated probes and streptavidin-coated magnetic particles. Captured fragments were cloned into plasmids, screened for microsatellites with a simple PCR reaction, and select plasmids then sequenced. PCR primers were designed and optimized for robust amplification, and nine primers have been further optimized for multiplex reactions with fluorescent primers. These nine loci are variable with an average of 6.11 alleles per locus and an average heterozygosity of 0.61. [source]

Dinucleotide microsatellite loci isolated from flowering dogwood (Cornus florida L.)

MOLECULAR ECOLOGY RESOURCES, Issue 2 2002
Paul R. Cabe
Abstract We report eight variable dinucleotide microsatellite loci cloned from flowering dogwood (Cornus florida L.) using a biotin enrichment protocol. Degenerate oligonucleotide primer-polymerase chain reaction (DOP-PCR) was used to generate a population of DNA fragments, from which adenine-cytosine dinucleotide (AC) and adenine-guanine dinucleotide (AG) repeats were captured using biotinylated probes and streptavidin coated magnetic particles. The captured fragments were cloned into plasmids, and the plasmid library was screened for microsatellites using a simple PCR technique. Selected plasmids were sequenced, and PCR primers were designed and optimized using a thermal-gradient thermocycler. The loci reported are highly variable with an average of 9.25 allele per locus and an average heterozygosity of 0.84. [source]

Preparation and characterization of crosslinked resins containing ferrite particles

POLYMER ENGINEERING & SCIENCE, Issue 10 2008
Luiz Claudio de Santa Maria
Micrometer-sized magnetic particles hosted on network material were successfully prepared by a simple chemical process (ion exchange followed by co-precipitation) from commercial styrene-divinylbenzene copolymers. Energy dispersive X-ray spectroscopy (EDS) coupled to scanning electron microscopy (SEM) allowed the observation of submicron particles. All the produced spherical beads have presented metallic particles (NiFe2O4, CuFe2O4, CoFe2O4, or MnFe2O4), either as isolated particles or agglomerates, located on their external and internal (within pores) The thermal stability of the composites, evaluated by thermogravimetric techniques, were found to be dependent on the amount of ferrite particles incorporated into them. POLYM. ENG. SCI., 2008. © 2008 Society of Plastics Engineers. [source]

Novel highly elastic magnetic materials for dampers and seals: Part I. Preparation and characterization of the elastic materials

POLYMERS FOR ADVANCED TECHNOLOGIES, Issue 11 2007
S. Abramchuk
Abstract The new generation of magnetic elastomers represents a new type of composites, consisting of small (mainly nano and micron-sized) magnetic particles dispersed in a highly elastic polymeric matrix. The combination of polymers with magnetic materials displays novel and often enhanced properties. Highly elastic magnetic composites are quite new and understanding of the behavior of these materials depending on the composition, external conditions, and the synthesis processes is still missing. Thus, the aim of this work is the study of fundamental principles governing the preparation of these materials as well as their structure and elastic properties. Copyright © 2007 John Wiley & Sons, Ltd. [source]

Novel highly elastic magnetic materials for dampers and seals: part II.

POLYMERS FOR ADVANCED TECHNOLOGIES, Issue 7 2007
Material behavior in a magnetic field
Abstract The combination of polymers with magnetic particles displays novel and often enhanced properties compared to the traditional materials. They can open up possibilities for new technological applications. The magnetic field sensitive elastomers represent a new type of composites consisting of small particles, usually from nanometer range to micron range, dispersed in a highly elastic polymeric matrix. In this paper, we show that in the presence of built-in magnetic particles it is possible to tune the elastic modulus by an external magnetic field. We propose a phenomenological equation to describe the effect of the external magnetic field on the elastic modulus. We demonstrate the engineering potential of new materials on the examples of two devices. The first one is a new type of seals fundamentally different from those used before. In the simplest case, the sealing assembly includes a magnetoelastic strip and a permanent magnet. They attract due to the magnetic forces. This ensures that due to high elasticity of the proposed composites and good adhesion properties, the strip of magnetoelastic will adopt the shape of the surface to be sealed, this fact leading to an excellent sealing. Another straightforward application of the magnetic composites is based on their magnetic field dependent elastic modulus. Namely, we demonstrate in this paper the possible application of these materials as adjustable vibration dampers. Copyright © 2007 John Wiley & Sons, Ltd. [source]

Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing

BIOTECHNOLOGY & BIOENGINEERING, Issue 1 2009
Tomoko Yoshino
Abstract There is a high demand for inexpensive and high-throughput DNA sequencing technologies in molecular biology and applied biosciences. In this study, novel nano-sized magnetic particles displaying enzymes for pyrosequencing, a rather novel bioluminometric DNA sequencing method based on the sequencing-by-synthesis principle by employing a cascade of several enzymatic reactions, was developed. A highly thermostable enzyme, pyruvate phosphate dikinase (PPDK) which converts PPi to ATP was successfully expressed onto bacterial magnetic particles (BacMPs) using a novel protein display system of Magnetospirillum magneticum AMB-1. The enzymatic stability of BacMPs displaying PPDK (PPDK-BacMPs) to pH and temperature was evaluated and its broad range of properties was shown. Subsequently, PPDK-BacMPs were applied in pyrosequencing and a target oligonucleotide was successfully sequenced. The PPDK enzyme displayed on BacMPs was shown to be recyclable in each sequence reaction as they can be manipulated by magnetic force. It was concluded that nano-sized PPDK-BacMPs are useful for the scale down of pyrosequencing reaction volumes, thus, permitting high-throughput. The recycling of enzymes was also shown to be promising and applicable for the development of an inexpensive DNA sequencing at a low running cost. Biotechnol. Bioeng. 2009;103: 130,137. © 2008 Wiley Periodicals, Inc. [source]

Modeling of Product Removal during Enzymatic Conversions by Using Affinity Molecules

BIOTECHNOLOGY PROGRESS, Issue 6 2007
Daniël G. R. Halsema
The feasibility of using magnetic particles for in-line product isolation during enzymatic conversion was studied. A comparison was made between a process based on magnetic particles and a conventional adsorption column. The enzymatic reaction was described by two consecutive first-order reactions (synthesis and subsequent hydrolysis), while the adsorption of substrate and product was described by multicomponent Langmuir isotherms. The yield as well as synthesis/hydrolysis ratio were calculated for various system characteristics. The results show that magnetic particles are very effective when the affinity with the particles is specific and for enzymatic conversions involving low ratios of the rate of synthesis versus the rate of hydrolysis. For slow conversions and for low specific affinity molecules column separations are more appropriate. [source]

Synthesis of Novel Porous Magnetic Silica Microspheres as Adsorbents for Isolation of Genomic DNA

Functionalization of Gold and Glass Surfaces with Magnetic Nanoparticles Using Biomolecular Interactions

BIOTECHNOLOGY PROGRESS, Issue 1 2006
Bala G. Nidumolu
Advances in nanotechnology have enabled the production and characterization of magnetic particles with nanometer-sized features that can be functionalized with biological recognition elements for numerous applications in biotechnology. In the present study, the synthesis of and interactions between self-assembled monolayers (SAMs) on gold and glass surfaces and functionalized magnetic nanoparticles have been characterized. Immobilization of 10,15 nm streptavidin-functionalized nanoparticles to biotinylated gold and glass surfaces was achieved by the strong interactions between biotin and streptavidin. Fluorescent streptavidin-functionalized nanoparticles, biotinylated surfaces, and combinations of the two were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and electron and fluorescent microscopy to confirm that little or no functionalization occurred in nonbiotinylated regions of the gold and glass surfaces compared to the biotinylated sites. Together these techniques have potential use in studying the modification and behavior of functionalized nanoparticles on surfaces in biosensing and other applications. [source]

A Novel Magnetic Affinity Support for Protein Adsorption and Purification

BIOTECHNOLOGY PROGRESS, Issue 1 2001
Xiao-Dong Tong
A novel magnetic support was prepared by an oxidization-precipitation method with poly(vinyl alcohol) (PVA) as the entrapment material. Transmission electron microscopy indicated that the magnetic particles had a core-shell structure, containing many nanometer-sized magnetic cores stabilized by the cross-linked PVA. The particles showed a high magnetic responsiveness in magnetic field, and no aggregation of the particles was observed after the particles had been treated in the magnetic field. These facts indicated that the particles were superparamagnetic. Cibacron blue 3GA (CB) was coupled to the particles to prepare a magnetic affinity support (MAS) for protein adsorption. Lysozyme was used as a model protein to test the adsorption properties of the MAS. The adsorption equilibrium of lysozyme to the MAS was described by the Langmuir-type isotherm. The capacity for lysozyme adsorption was more than 70 mg/g MAS (wet weight) at a relatively low CB coupling density (3,5 ,mol/g). In addition, 1.0 M NaCl solution could be used to dissociate the adsorbed lysozyme. Finally, the MAS was recycled for the purification of alcohol dehydrogenase (ADH) from clarified yeast homogenates. Under proper conditions, the magnetic separation yielded over 5-fold purification of the enzyme with 60% recovery of the enzyme activity. [source]