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M. Domestica (m + domestica)
Selected AbstractsIncreased toxicity to invertebrates associated with a mixture of atrazine and organophosphate insecticidesENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 7 2002Troy D. Anderson Abstract This study examined the joint toxicity of atrazine and three organophosphate (OP) insecticides (chlorpyrifos, methyl parathion, and diazinon) exposed to Hyalella azteca and Musca domestica. A factorial design was used to evaluate the toxicity of binary mixtures in which the lethal concentration/lethal dose (LC1/LD1, LC5/LD5, LC15/LD15, and LC50/LD50) of each OP was combined with atrazine concentrations of 0, 10, 40, 80, and 200 ,g/L for H. azteca and 0, 200, and 2,000 ng/mg for M. domestica. Atrazine concentrations (>40 ,g/L) in combination with each OP caused a significant increase in toxicity to H. azteca compared with the OPs dosed individually. Acetylcholinesterase (AChE) activity also was examined for the individual OPs with and without atrazine treatment. Atrazine in combination with each of the OPs resulted in a significant decrease in AChE activity compared with the OPs dosed individually. In addition, H. azteca that were pretreated with atrazine (>40 ,g/L) were much more sensitive to the OP insecticides compared with H. azteca that were not pretreated with atrazine before being tested. Topical exposure to atrazine concentrations did not significantly increase OP toxicity to M. domestica. The results of this study indicate the potential for increased toxicity in organisms exposed to environmental mixtures. [source] Insecticidal activity of garlic juice in two dipteran pestsAGRICULTURAL AND FOREST ENTOMOLOGY, Issue 1 2006Gareth M. Prowse Abstract 1Botanical products excluding pyrethroids constitute a small, but growing portion of the U.K. pesticides market. With increasing legislative pressure upon chemical pesticides such as organophosphates, interest in this sector is increasing steadily. 2Garlic Allium sativum L. juices and extracts form the basis of several commercially available pest control products, but the performance of these products is variable, possibly due to lack of quality control upon batches of materials in the manufacturing process. 3Some garlic products designed for use in the food industry are subjected to rigorous batch-control to ensure organoleptic consistency. We studied the insecticidal efficacy of a commercially produced food grade garlic juice using two target dipteran pests, Delia radicum (L.) and Musca domestica L. 4Exposure of the two species to different concentrations of garlic juice revealed variability in insecticidal effect across life stages. LC50 values recorded for D. radicum were: eggs (7-day exposure) 0.8%; larvae (24-h exposure) 26.4%; larvae (48-h exposure) 6.8%; and adults (24-h exposure) 0.4%. LC50 values recorded for M. domestica were: eggs (7-day exposure) 1.6%; larvae (24-h exposure) 10.1%; larvae (24-h exposure) 4.5%; and adults (24-h exposure) 2.2%. 5Mortality rates caused by the garlic juice were comparable with those obtained with the organophosphate pesticide Birlane®, indicating parity of effect at various concentrations depending on life stage. 6We conclude that this product may provide an effective, naturally-derived insecticide for use in agricultural systems against dipteran pests. [source] Early ontogeny and placentation of the grey short-tailed opossum, Monodelphis domestica (Didelphidae: Marsupialia): contribution to the reconstruction of the marsupial morphotypeJOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, Issue 3 2001Zeller This study provides new findings on the placenta of Monodelphis domestica and a reconstruction of the marsupial morphotype. To achieve this, early ontogeny and placentation of the grey short-tailed opossum, M. domestica, from 3 h after copulation until birth (day 15), were studied and compared with other mammals. Both the ultrastructure and histochemistry of egg membranes, foetal membranes, oviduct and uterus were examined. The results of this study provide the first detailed ultrastructural description of a trophoblastic syncytium in a marsupial. In addition, this is the first original documentation of an invasive trophectoderm and an inflammatory reaction at parturition in M. domestica. These findings were compared with literature data and included into the reconstruction of the marsupial morphotype. Based on marsupial phylogeny as proposed by Luckett (J. Mammal. Evol. 2, 255,283, 1994), characters that are consistent at least within didelphids and dasyurids were determined to be characters of the marsupial morphotype. These characters are a central yolk separated from the peripheral yolk-poor cytoplasm in the unfertilized oocyte, the presence of a zona pellucida, a mucoid coat and a shell coat, the absence of a corona radiata, oviductal mucoid secretion, no shell secretion distal to the isthmus of the oviduct, uterine shell secretion, a short tubal passage (1 day at maximum), the apposition of blastomeres to the zona pellucida prior to intercellular association, the absence of a morula stage, the polarity of the zygotic yolk, the localized segmentation of deutoplasm (yolk) during the first cleavage and subsequent extrusion of yolk vesicles during the first two cleavage stages. With regard to the marsupial morphotype, the non-polarized yolk distribution in the zygote [Hartman (J. Morphol. 27, 1,84, 1916); McCrady (Am. Anat. Mem. 16, 1,233, 1938)] is a derived character of Didelphis virginiana. Didelphis virginiana [Hartman (J. Morphol. 27, 1,84, 1916); Hartman (J. Morphol. 32, 1,139, 1919); McCrady (Am. Anat. Mem. 16, 1,233, 1938)] and Didelphis marsupialis (Hill, Q. J. Micr. Sci. 63, 91,139, 1918) share the synapomorphous reduction of deutoplasmolysis to a generalized extrusion of vesicles. The absence of separated yolk and consequently a cleavage without yolk extrusion (Renfree and Lewis, Reprod. Fert. Dev. 8, 725,742, 1996) are apomorphies of macropodids. This is possibly correlated with the association of blastomeres in early cleavage stages (Renfree and Lewis, Reprod. Fert. Dev. 8, 725,742, 1996). A yolk sac placenta and a vascularized allantochorion can be assumed for part of the ontogeny in the marsupial morphotype, irrespective of the formation of an allantoic placenta at near term stages. The character polarization of the mode of placentation and parturition needs further investigation. Frühe Ontogenie und Plazentation der grauen Hausspitzmausbeutelratte, Monodelphis domestica (Didelphidae: Marsupialia): Ein Beitrag zur Rekonstruktion des Grundplans der Marsupialia Die vorliegende Arbeit beschreibt die frühe Ontogenese und Plazentation von 3 Stunden nach der Kopulation bis zur Geburt der Beutelratte Monodelphis domestica. Es wird die Ultrastruktur und Histochemie der Eihäute, der Fetalmembranen, des Oviductes und des Uterus beschrieben. Erstmalig wird die Ultrastruktur eines trophoblastischen Syncytiums bei einem Beuteltier beschrieben. Weiterhin wird ein invasives Trophektoderm und eine Entzündungsreaktion zum Zeitpunkt der Geburt bei M. domestica festgestellt. Die Befunde dieser Studie und Literaturdaten werden verglichen und in eine Grundplanrekonstruktion integriert. Merkmale, die mindestens zwischen Vertretern der Didelphidae und Dasyuridae übereinstimmen, werden basierend auf dem phylogenetischen System der Marsupialia nach Luckett, J. Mammal. Evol. 2, 255,283, 1994, für den Grundplan der Marsupialia angenommen. Diese Merkmale sind zentral separierter Dotter und peripheres dotterarmes Zytoplasma in der unbefruchteten Eizelle, das Vorhandensein von Zona pellucida, Mucoidschicht und Schalenhaut, das Fehlen einer Corona radiata, die Mucoidsekretion durch den Oviduct, die Schalensekretion durch den Uterus und nicht distal der Isthmusregion des Oviductes, eine kurze Tubenwanderung (maximal einen Tag), die Anlagerung der Blastomeren an die Zona pellucida vor der interzellulären Verbindung, das Fehlen eines Morulastadiums, die Dotterpolarität in der Zygote, die lokale Dotterabtrennung bei der ersten Teilung und die anschließende Dotterextrusion während der ersten beiden Teilungen. In Bezug auf den Grundplan der Marsupialia ist die unpolare Dotterverteilung in der Zygote ein abgeleitetes Merkmal von Didelphis virginiana. Didelphis virginiana und Didelphis marsupialis teilen als Synapomorphie die Reduktion der Deutoplasmolyse auf eine generelle Vesikelextrusion. Das Fehlen separierten Dotters in der Oocyte und die resultierende Furchung ohne Dotterextrusion [Renfree and Lewis, Reprod. Fert. Dev. 8, 725,742, 1996] ist eine Apomorphie der Macropodidae. Hiermit hängt möglicherweise die frühe Zusammenlagerung der Blastomeren zusammen [Renfree and Lewis, Reprod. Fert. Dev. 8, 725,742, 1996]. Ein vaskularisiertes Allantochorion und eine Dottersackplazenta können für einen Teil der Ontogenese im Grundplan der Marsupialia angenommen werden. Ob das Allantochorion neben der Respiration auch dem Stoffaustausch diente ist unklar. Die Lesrichtung für den Modus der Plazentation und der Geburt bedarf weiterer Untersuchungen. [source] Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditionsMEDICAL AND VETERINARY ENTOMOLOGY, Issue 3 2008D. TRAVERSA Abstract A polymerase chain reaction (PCR)-based assay was used for the specific detection of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) in order to identify the intermediate hosts of both nematode species under field conditions. A total of 1087 netted and 165 laboratory-bred flies were tested. Flies were identified as Musca domestica Linnaeus 1758, Musca autumnalis De Geer 1776, Haematobia irritans (Linnaeus 1758), Haematobia titillans (De Geer 1907) and Stomoxys calcitrans (Linnaeus 1758) (Muscidae). Genomic DNA was extracted from pools of fly heads, thoraces and abdomens, and 703 samples were subjected to a duplex two-step semi-nested PCR assay to specifically detect diagnostic regions within the ribosomal ITS2 sequence of both H. microstoma and H. muscae. Stomoxys calcitrans specimens were positive for H. microstoma DNA and M. domestica specimens were positive for H. muscae DNA. In particular, PCR-positive samples derived from both farm-netted and laboratory-bred flies. The present study represents the first evidence of the vectorial competence of different fly species as intermediate hosts of Habronema stomachworms under field conditions. We discuss the roles of S. calcitrans and M. domestica in transmitting H. microstoma and H. muscae. [source] Development of PDF-immunoreactive cells, possible clock neurons, in the housefly Musca domesticaMICROSCOPY RESEARCH AND TECHNIQUE, Issue 2 2003Elzbieta Pyza Abstract Even though the housefly Musca domestica shows clear circadian rhythms in its behavioural and physiological processes, a circadian pacemaker system controlling these rhythms has not yet been described morphologically in this species. In M. domestica, neurons immunoreactive to pigment-dispersing factor (PDF), a neurotransmitter/neuromodulator of circadian information arising from a circadian clock and transmitted to target cells, are similar in their number and distribution to the PDF neurons of Drosophila melanogaster. In D. melanogaster these neurons co-localize PER protein and have been identified as clock neurons in that species. Here we report PDF-immunoreactive cells in the housefly's brain during postembryonic development in the larval and pupal stages, as well as in the adult fly soon after eclosion. In the housefly's brain, there are three groups of PDF-immunoreactive neurons: two groups with small (sPDFMe) and large (lPDFMe) cell bodies in the proximal medulla of the optic lobe; and one group in the dorsal protocerebrum (PDFD). Three out of four sPDFMe can be detected during the first hour of larval development, but the fourth sPDFMe is observed in the larva only from 48 hours after hatching, along with five lPDFMe neurons, seen first as two subgroups, and three out of four PDFD neurons. During postembryonic development these neurons show changes in their structure and immunoreactivity. New PDF neurons are observed during pupal development but these neurons mostly do not survive into adulthood. In the adult fly's brain, the PDF neurons have also been examined in double-labelled preparations made with a second antibody directed against the product of one of several clock genes: period (per), timeless (tim), or cryptochrome (cry). Among them, only immunoreactivity to CRY-like protein has been detected in the brain of M. domestica and has shown a daily rhythm in its concentration, as examined immunocytochemically. CRY was co-localized with PDF in the sPDFMe of the housefly's brain fixed during the day. The possibility that the sPDFMe neurons are the housefly's clock neurons is discussed. Microsc. Res. Tech. 62:103,113, 2003. © 2003 Wiley-Liss, Inc. [source] Inheritance of beta-cypermethrin resistance in the housefly Musca domestica (Diptera: Muscidae)PEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 2 2008Lan Zhang Abstract BACKGROUND: Beta-cypermethrin, a synthetic pyrethroid insecticide, was applied frequently in the control of health pests including houseflies, Musca domestica L., in China. However, different levels of resistance to beta-cypermethrin were monitored in field strains of houseflies. A strain of M. domestica, 4420-fold resistant to beta-cypermethrin after continuous 25 generations of selection, was used in this paper to determine the mode of inheritance of pyrethroid resistance. RESULTS: The estimated realized heritability (h2) of beta-cypermethrin resistance was 0.30 in this resistant strain. Results of bioassays showed no significant difference in values of LD50 and slope of log dose-probit lines between reciprocal progenies F1 and F,1, and yielded values of , 0.10 (F1) and , 0.11 (F,1) for the degree of dominance (D). Chi-square analysis from responses of self-bred and backcross progenies (F2, BC1 and BC2 respectively) indicated that the null hypothesis, a single gene responsible for resistance, was accepted. The minimum number of independent segregation genes was 0.93 for F1 by Lande's method. CONCLUSION: It was concluded that beta-cypermethrin resistance in the housefly was inherited as a single, major, autosomal and incompletely recessive factor. These results would provide the basic information for pest management programmes. Copyright © 2007 Society of Chemical Industry [source] Compounds from Ageratum conyzoides: isolation, structural elucidation and insecticidal activityPEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 6 2007Márcio D Moreira Abstract This work aimed at identifying plant compounds with insecticidal activity against Diaphania hyalinata (L.) (Lepidoptera: Pyralidae), Musca domestica (L.) (Diptera: Muscidae), Periplaneta americana (L.) (Blattodea: Blattidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetaefolia L.), Jimson weed (Datura stramonium L.), ,baleeira' herb (Cordia verbenaceae L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.) and billy goat weed (Ageratum conyzoides L.). Firstly, the insecticidal activities of hexane and ethanol plant extracts were evaluated against adults of R. dominica. Among them, only the hexane extract of A. conyzoides showed insecticidal activity. The hexane extract of this plant species was therefore fractionated by silica gel column chromatography to isolate and purify its bioactive chemical constituents. Three compounds were identified using IR spectra, 1H NMR, 13C NMR, HMBC and NOE after gel chromatography: 5,6,7,8,3,, 4,, 5,-heptamethoxyflavone, 5,6,7,8,3,-pentamethoxy-4,, 5,-methylenedioxyflavone and coumarin. The complete assignment of 13C NMR to 5,6,7,8,3,-pentamethoxy-4,, 5,-methylenedioxyflavone was successfully made for the first time. 5,6,7,8,3,-Pentamethoxy-4,, 5,-methylenedioxyflavone did not show any insecticidal activity against the four insect species tested. 5,6,7,8,3,, 4,, 5,-Heptamethoxyflavone showed low activity against D. hyalinata and R. dominica and was not toxic to M. domestica or P. americana. In contrast, coumarin showed insecticidal activity against all four insect pest species tested, with the following order of susceptibility: R. dominica < P. americana < D. hyalinata < M. domestica after 24 h exposure. Copyright © 2007 Society of Chemical Industry [source] | ||||||||||