Home  About us  Contact
 

Loci Isolated (locus + isolated)

Distribution by Scientific Domains
Life Sciences100%

Kinds of Loci Isolated

  • microsatellite locus isolated
    		•

    Selected Abstracts

    Twenty-one novel microsatellite DNA loci isolated from the Puget Sound white-crowned sparrow, Zonotrichia leucophrys pugetensis

    MOLECULAR ECOLOGY RESOURCES, Issue 3 2009
    ANGELIKA POESEL
    Abstract The white-crowned sparrow, Zonotrichia leucophrys, has served as a model species for studies of song and reproductive physiology. Here, we describe primers for 21 novel microsatellite loci isolated from the Puget Sound subspecies, Zonotrichia leucophrys pugetensis, which will be useful for parentage and population genetic analyses. Based on genotypes from seven to 22 adult birds from one population, the average number of alleles per locus was 10.9 (four to 21 alleles) and observed heterozygosity varied from 0.50 to 1.00. All loci also amplified products in at least one of three other passerine species tested. [source]

    Characterization of polymorphic microsatellite markers in the Dalmatian wall lizard Podarcis melisellensis (Squamata: Lacertidae)

    MOLECULAR ECOLOGY RESOURCES, Issue 1 2009
    K. HUYGHE
    Abstract We describe polymerase chain reaction primers and amplification conditions for 13 highly polymorphic microsatellite DNA loci isolated from the Dalmatian wall lizard, Podarcis melisellensis. The number of alleles per locus ranged from 12 to 41, with levels of observed heterozygosity between 0.62 and 0.94. Most of these loci were successfully cross-amplified in the closely related species P. sicula, but levels of polymorphism were always lower. [source]

    A large panel of novel microsatellite markers for the bank vole (Myodes glareolus)

    MOLECULAR ECOLOGY RESOURCES, Issue 5 2008
    K. RIKALAINEN
    Abstract We describe a set of 66 highly polymorphic microsatellite loci isolated from the bank vole, Myodes (Clethrionomys) glareolus. These microsatellites were characterized for a long-term study on periodically fluctuating density of the bank vole population in Central Finland. We detected six to 38 alleles per locus in the population sampled at two different density phases, and the levels of observed and expected heterozygosities varied between 0.17 and 1.00, and between 0.72 and 0.95, respectively. This microsatellite panel serves as an informative tool for population and molecular genetic studies. [source]

    Polymorphic microsatellite loci from northern and Mexican corn rootworms (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp.

    MOLECULAR ECOLOGY RESOURCES, Issue 3 2008
    ERIC R. WAITS
    Abstract The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we present 14 polymorphic microsatellite loci isolated from libraries constructed using pooled northern and Mexican corn rootworm genomic DNA. Polymorphism in other Diabrotica, including the banded cucumber beetle, southern corn rootworm and western corn rootworm, is described. [source]

    Isolation and characterization of 17 polymorphic microsatellites in grass carp

    MOLECULAR ECOLOGY RESOURCES, Issue 6 2007
    JIA LE LI
    Abstract Here we report the isolation and characterization of 17 polymorphic loci isolated from a partial genomic DNA library of grass carp (Ctenopharyngodon idellus) enriched for CA repeats. We tested variability of these microsatellites on 24 unrelated individuals collected in China. All microsatellites were polymorphic. The average allele number was 7.9 per locus, ranging from four to 13. The observed heterozygosity was from 0.46 to 0.88 with an average of 0.71, whereas the average expected heterozygosity was 0.78. Sixteen of the 17 microsatellites conformed to Hardy,Weinberg equilibrium, and inherited independently. These microsatellites can be used to study genetic diversity and population structure of wild populations, and facilitate selective breeding of cultured broodstocks. [source]

    Variable microsatellite loci isolated from the azure damselfly, Coenagrion puella (L.) (Zygoptera; Coenagrionidae)

    MOLECULAR ECOLOGY RESOURCES, Issue 5 2007
    CHRIS D. LOWE
    Abstract We isolated and characterized 10 polymorphic microsatellite loci from the azure damselfly Coenagrion puella (Zygoptera; Coenagrionidae) as part of a study assessing reproductive success and genetic structure in an isolated population of this species. Levels of genetic diversity were assessed in 50 individuals collected from Queen Elizabeth Country Park, Hampshire, UK. The number of alleles per microsatellite loci ranged from three to 22 and the observed and expected heterozygosities varied between 0.26 and 0.84 and between 0.23 and 0.91, respectively. Two loci showed significant (P < 0.05) heterozygote deficits, likely because of null (non-amplifying) alleles; one pair of loci was in linkage disequilibrium. [source]

    Isolation and characterization of microsatellite loci in a cooperatively breeding corvid, the American crow (Corvus brachyrhynchos)

    MOLECULAR ECOLOGY RESOURCES, Issue 1 2007
    L. A. SCHOENLE
    Abstract We describe 11 microsatellite loci isolated from the American crow (Corvus brachyrhynchos), the most wide-spread cooperatively breeding bird in North America. Polymorphism at these loci ranged from four to 43 alleles, and observed heterozygosities from 0.53 to 0.92. Genetic parentage analyses using these markers will allow us to describe the mating system of this common but socially complex species, and to interpret their behavioural interactions in light of relatedness. [source]

    Isolation of microsatellite loci and cross-species amplifications in three gobiid fish of the genus Pomatoschistus

    MOLECULAR ECOLOGY RESOURCES, Issue 3 2006
    PATRICK BERREBI
    Abstract Gobiids of the genus Pomatoschistus are increasingly being investigated as models for adaptation to coastal environments and for mating system studies. Among the dozen currently analysed species, microsatellite primers have been characterized only for Pomatoschistus minutus. This paper describes seven new polymorphic loci isolated from Pomatoschistus marmoratus and Pomatoschistus microps, two species that hybridize. Cross-species amplification was tested for these new loci, together with seven already published P. minutus loci. Systematic amplification of samples of each of the three species provided a first indication of their polymorphism. [source]

    Development and characterization of microsatellite markers for a monoecious Ficus species, Ficus insipida, and cross-species amplification among different sections of Ficus

    MOLECULAR ECOLOGY RESOURCES, Issue 3 2006
    H. VIGNES
    Abstract The animal-mediated pollination and seed-dispersal mutualisms of Ficus species give them key roles in tropical ecosystems, but may make them vulnerable to habitat fragmentation. The development of highly polymorphic markers is needed to analyse their genetic diversity and investigate the effects of fragmentation on gene flow. Of thirteen microsatellite loci isolated from Ficus insipida, a monoecious species in French Guiana, eleven were polymorphic (two to six alleles each). High levels of variation were found among loci; expected heterozygosities ranged from 0.151 to 0.715. All markers revealed a broad cross-species affinity when tested in 23 other Ficus species. [source]

    Characterization of microsatellite loci isolated in midget faded rattlesnake (Crotalus viridis concolor)

    MOLECULAR ECOLOGY RESOURCES, Issue 2 2005
    S. J. OYLER-MCCANCE
    Abstract Primers for five polymorphic microsatellite loci were developed for the midget faded rattlesnake (Crotalus viridis concolor), a rare subspecies of western rattlesnake (Crotalus viridus) found only in parts of Wyoming, Colorado, and Utah. Five polymorphic microsatellites were isolated, four of which had relatively high levels of diversity (eight to nine alleles). We found only two departures from Hardy,Weinberg equilibrium and they occurred in different loci, so null alleles are likely not a problem. Moreover, we found that no two loci were linked. These loci will be applicable for population genetic analysis and perhaps analysis of paternity and mating systems. [source]

    Microsatellite loci isolated from the Caribbean coral, Montastraea annularis

    MOLECULAR ECOLOGY RESOURCES, Issue 1 2004
    E. G. Severance
    Abstract We report the isolation and characterization of seven microsatellite loci from the Caribbean reef-building coral, Montastraea annularis. All loci are polymorphic with allele numbers ranging from five to 31 and observed heterozygosities from 0.17 to 0.89. These loci can be used in assessing gene flow patterns and diversity of this stony coral species both for local coral reef management purposes as well as for elucidating population connectivity within the greater Caribbean basin. These markers should also be applicable to other species of Montastraea and for resolving taxonomic relationships within the M. annularis species complex. [source]

    Polymorphic tetranucleotide microsatellite DNA loci from the southern dusky salamander (Desmognathus auriculatus)

    MOLECULAR ECOLOGY RESOURCES, Issue 4 2003
    Dean A. Croshaw
    Abstract We describe polymerase chain reaction (PCR) primers and amplification conditions for seven tetranucleotide microsatellite DNA loci isolated from the southern dusky salamander (Desmognathus auriculatus). Primers were tested on 16 individuals from one population in Aiken County, South Carolina. We detected an average of 6.57 alleles per locus, an observed heterozygosity range of 0.44,0.94, and high polymorphic information contents (mean of 0.68). [source]

    Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin)

    MOLECULAR ECOLOGY RESOURCES, Issue 2 2003
    J. Susanne Hauswaldt
    Abstract We describe polymerase chain recation (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the Diamondback terrapin (Malaclemys terrapin). The PCR primers were tested on 21 terrapins from Cape Romain, SC, USA. The microsatellite primers developed yielded a high number of alleles (8,14) and high observed heterozygosities (0.57,1.0). [source]

    Polymorphic microsatellite loci in Plantago lanceolata

    MOLECULAR ECOLOGY RESOURCES, Issue 1 2003
    Marie L. Hale
    Abstract The genus Plantago is particularly interesting for evolutionary studies because of its wide range of mating systems. We have developed primers for five highly polymorphic microsatellite loci isolated from P. lanceolata. All five loci amplified and were polymorphic in the two populations examined, Lowsteads Beach in the United Kingdom and Duke in the United States. These new markers will allow a comparison of population structure between the outcrossing species P. lanceolata, and the highly selfing species P. major. [source]

    Characterization of 22 microsatellites loci from the endangered Houbara bustard (Chlamydotis undulata undulata)

    MOLECULAR ECOLOGY RESOURCES, Issue 4 2002
    Faiza Chbel
    Abstract Here we present a new set of 22 microsatellite loci isolated from Chlamydotis undulata undulata, an endangered Houbara bustard found across North Africa. The number of alleles per locus ranged from one to nine, and heterozygosities ranged from 0.167 to 0.944. Total exclusionary probabilities using these loci for the first and the second parent were 0.992932 and 0.999915, respectively. Successful cross-amplification was observed in eight other Otididae species (12,22 of the 22 loci). These microsatellite markers are powerful tools for genetic identification, paternity assignment and population genetic studies. [source]

    Dinucleotide microsatellite loci isolated from flowering dogwood (Cornus florida L.)

    MOLECULAR ECOLOGY RESOURCES, Issue 2 2002
    Paul R. Cabe
    Abstract We report eight variable dinucleotide microsatellite loci cloned from flowering dogwood (Cornus florida L.) using a biotin enrichment protocol. Degenerate oligonucleotide primer-polymerase chain reaction (DOP-PCR) was used to generate a population of DNA fragments, from which adenine-cytosine dinucleotide (AC) and adenine-guanine dinucleotide (AG) repeats were captured using biotinylated probes and streptavidin coated magnetic particles. The captured fragments were cloned into plasmids, and the plasmid library was screened for microsatellites using a simple PCR technique. Selected plasmids were sequenced, and PCR primers were designed and optimized using a thermal-gradient thermocycler. The loci reported are highly variable with an average of 9.25 allele per locus and an average heterozygosity of 0.84. [source]

    Polymorphic microsatellite loci for the common marmoset (Callithrix jacchus) designed using a cost- and time-efficient method

    AMERICAN JOURNAL OF PRIMATOLOGY, Issue 9 2008
    M. Raveendran
    Abstract We describe a cost- and time-efficient method for designing new microsatellite markers in any species with substantial genomic DNA sequence data available. Using this technique, we report 14 new polymorphic dinucleotide microsatellite loci isolated from the common marmoset. The relative yield of new polymorphisms was higher with less labor than described in previous marmoset studies. Of 20 loci initially evaluated, 14 were polymorphic and amplified reliably (70% success rate). The number of alleles ranged from 3 to 9 with heterozygosity varying from 0.48 to 0.83. Am. J. Primatol. 70:906,910, 2008. © 2008 Wiley-Liss, Inc. [source]

    Pirate ants (Polyergus breviceps) and sympatric hosts (Formica occulta and Formica sp. cf. argentea): host specificity and coevolutionary dynamics

    BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 4 2007
    JEREMY M. BONO
    The pace and trajectory of coevolutionary arms races between parasites and their hosts are strongly influenced by the number of interacting species. In environments where a parasite has access to more than one host species, the parasite population may become divided in preference for a particular host. In the present study, we show that individual colonies of the pirate ant Polyergus breviceps differ in host preference during raiding, with each colony specializing on only one of two available Formica host species. Moreover, through genetic analyses, we show that the two hosts differ in their colony genetic structure. Formica occulta colonies were monogynous, whereas Formica sp. cf. argentea colonies were polygynous and polydomous (colonies occupy multiple nest sites). This difference has important implications for coevolutionary dynamics in this system because raids against individual nests of polydomous colonies have less impact on overall host colony fitness than do attacks on intact colonies. We also used primers that we designed for four microsatellite loci isolated from P. breviceps to verify that colonies of this species, like other pirate ants, are comprised of simple families headed by one singly mated queen. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society, 2007, 91, 565,572. [source]