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Local Differences (local + difference)
Selected AbstractsFas ligand expressed in colon cancer is not associated with increased apoptosis of tumor cells in vivoINTERNATIONAL JOURNAL OF CANCER, Issue 2 2003Aileen Houston Abstract Expression of Fas ligand (FasL/CD95L) may help to maintain colon cancers in a state of immune privilege by inducing apoptosis of antitumor immune effector cells. Colon tumor-derived cell lines appear to be relatively insensitive to apoptosis mediated by their own or exogenous FasL in vitro, despite expression of cell surface Fas. In our present study, we sought to investigate if FasL upregulated in human colon cancers leads to any increase in apoptosis of the tumor cells in vivo. FasL and Fas receptor (APO-1/CD95) expression by tumor cells were detected immunohistochemically. Apoptotic tumor cell death was detected by immunohistochemistry for caspase-cleaved cytokeratin-18. FasL expression did not correlate with the extent of apoptosis of tumor cells. There was no significant local difference in the frequency of apoptosis of tumor cells between tumor nests that expressed FasL (mean = 2.4%) relative to those that did not (mean = 2.6%) (p = 0.625, n = 10; Wilcoxon signed rank). FasL expressed by the tumor cells appeared to be functional, since FasL expression in tumor nests correlated with diminished infiltration of tumor-infiltrating lymphocytes (TILs). TILs were detected using immunohistochemistry for CD45. Expression of FasL by tumor nests was associated with a mean 4-fold fewer TILs relative to FasL-negative nests (range 2.4,33-fold, n = 10, p < 0.003). Together, our results indicate that colon tumors are insensitive to FasL-mediated apoptosis in vivo. © 2003 Wiley-Liss, Inc. [source] The micro-topography of the wetlands of the Okavango Delta, BotswanaEARTH SURFACE PROCESSES AND LANDFORMS, Issue 1 2005T. Gumbricht Abstract The surface of the 40 000 km2 Okavango alluvial fan is remarkably smooth, and almost everywhere lies within two to three metres of a perfectly smooth theoretical surface. Deviations from this perfect surface give rise to islands in the Okavango wetlands. This micro-topography was mapped by assigning empirical elevations to remotely sensed vegetation community classes, based on the observation that vegetation is very sensitive to small, local differences in elevation. Even though empirical, the method produces fairly accurate results. The technique allows estimation of depths of inundation and therefore will be applicable even when high resolution radar altimetry becomes available. The micro-topography has arisen as a result of clastic sedimentation in distributary channels, which produces local relief of less than two metres, and more importantly as a result of chemical precipitation in island soils, which produces similar local relief. The micro-topography is, therefore, an expression of the non-random sedimentation taking place on the fan. Volume calculations of islands extracted from the micro-topography, combined with estimates of current sediment in,ux, suggest that the land surface of the wetland may only be a few tens of thousands of years old. Constant switching of water distribution, driven by local aggradation, has distributed sediment widely. Mass balance calculations suggest that over a period of c. 150 000 years all of the fan would at one time or other have been inundated, and thus subject to sedimentation. Coalescing of islands over time results in net aggradation of the fan surface. The amount of vertical aggradation on islands and in channels is restricted by the water depth. Restricted vertical relief, in turn, maximizes the distribution of water, limiting its average depth. Aggradation in the permanent swamps occurs predominantly by clastic sedimentation. Rates of aggradation here are very similar to those in the seasonal swamps, maintaining the overall gradient, possibly because of the operation of a feedback loop between the two. The limited amount of local aggradation arising from both clastic and chemical sedimentation, combined with constant changes in water distribution, has resulted in a near-perfect conical surface over the fan. In addition to providing information on sedimentary processes, the micro-topography has several useful hydrological applications. Copyright © 2004 John Wiley & Sons, Ltd. [source] Spatial and temporal analysis of fMRI data on word and sentence readingEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 7 2007Sven Haller Abstract Written language comprehension at the word and the sentence level was analysed by the combination of spatial and temporal analysis of functional magnetic resonance imaging (fMRI). Spatial analysis was performed via general linear modelling (GLM). Concerning the temporal analysis, local differences in neurovascular coupling may confound a direct comparison of blood oxygenation level-dependent (BOLD) response estimates between regions. To avoid this problem, we parametrically varied linguistic task demands and compared only task-induced within-region BOLD response differences across areas. We reasoned that, in a hierarchical processing system, increasing task demands at lower processing levels induce delayed onset of higher-level processes in corresponding areas. The flow of activation is thus reflected in the size of task-induced delay increases. We estimated BOLD response delay and duration for each voxel and each participant by fitting a model function to the event-related average BOLD response. The GLM showed increasing activations with increasing linguistic demands dominantly in the left inferior frontal gyrus (IFG) and the left superior temporal gyrus (STG). The combination of spatial and temporal analysis allowed a functional differentiation of IFG subregions involved in written language comprehension. Ventral IFG region (BA 47) and STG subserve earlier processing stages than two dorsal IFG regions (BA 44 and 45). This is in accordance with the assumed early lexical semantic and late syntactic processing of these regions and illustrates the complementary information provided by spatial and temporal fMRI data analysis of the same data set. [source] Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neuronsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 10 2004Hasna Baloui Abstract The 67-kDa LR protein was originally discovered as a non-integrin laminin receptor. Several more recent in vitro studies demonstrated the function of 67-kDa LR and its related ,precursor' form 37-kDa LRP as receptors of cellular prion protein and their implication in abnormal prion protein propagation in vitro. In addition, expression of both proteins was shown to increase considerably in the brain of scrapie-infected mice and hamsters. While LRP/LR are thus likely to play important roles in neuronal cell adhesion, survival and homeostasis and during pathological disorders, little is known so far about their fine cellular distribution in adult central nervous system. Using immunocytochemistry and western blotting, we show here that the 67-kDa LR is the major receptor form in adult rat brain and spinal cord, expressed within the cytoplasm and at the plasma membrane of most neurons and in a subset of glial cells. The overall distribution of LR correlates well with that reported for laminin-1 but also with brain regions classically associated with prion-related neurodegeneration. In contrast to LR, the 37-kDa LRP form is much less abundant in adult than in postnatal central nervous system. Characterization of a novel antibody allowed us to study the distribution across tissues of cell membrane-associated LRP. Interestingly, this form is almost exclusively found on a subclass of parvalbumin-immunoreactive cortical interneurons known to degenerate during the early stages of Creutzfeldt-Jakob disease. Our demonstration of local differences in the expression of particular LRP/LR isoforms may be a first step towards unraveling their specific molecular interactions. [source] Effects of climate on population fluctuations of ibexGLOBAL CHANGE BIOLOGY, Issue 2 2008VIDAR GRØTAN Abstract Predicting the effects of the expected changes in climate on the dynamics of populations require that critical periods for climate-induced changes in population size are identified. Based on time series analyses of 26 Swiss ibex (Capra ibex) populations, we show that variation in winter climate affected the annual changes in population size of most of the populations after accounting for the effects of density dependence and demographic stochasticity. In addition, precipitation during early summer also influenced the population fluctuations. This suggests that the major influences of climate on ibex population dynamics operated either through loss of individuals during winter or early summer, or through an effect on fecundity. However, spatial covariation in these climate variables was not able to synchronize the population fluctuations of ibex over larger distances, probably due to large spatial heterogeneity in the effects of single climate variables on different populations. Such spatial variation in the influence of the same climate variable on the local population dynamics suggests that predictions of influences of climate change need to account for local differences in population dynamical responses to climatic conditions. [source] The role of the staff MFF in distributing NHS funding: taking account of differences in local labour market conditionsHEALTH ECONOMICS, Issue 5 2010Robert Elliott Abstract The National Health Service (NHS) in England distributes substantial funds to health-care providers in different geographical areas to pay for the health care required by the populations they serve. The formulae that determine this distribution reflect populations' health needs and local differences in the prices of inputs. Labour is the most important input and area differences in the price of labour are measured by the Staff Market Forces Factor (MFF). This Staff MFF has been the subject of much debate. Though the Staff MFF has operated for almost 30 years this is the first academic paper to evaluate and test the theory and method that underpin the MFF. The theory underpinning the Staff MFF is the General Labour Market method. The analysis reported here reveals empirical support for this theory in the case of nursing staff employed by NHS hospitals, but fails to identify similar support for its application to medical staff. The paper demonstrates the extent of spatial variation in private sector and NHS wages, considers the choice of comparators and spatial geography, incorporates vacancy modelling and illustrates the effect of spatial smoothing. Copyright © 2009 John Wiley & Sons, Ltd. [source] Euroethics,a database network on biomedical ethicsHEALTH INFORMATION & LIBRARIES JOURNAL, Issue 3 2006Ylva Gavel Background:,euroethics is a database covering European literature on ethics in medicine. It is produced within Eurethnet, a European information network on ethics in medicine and biotechnology. Objectives:, The aim of Euroethics is to disseminate information on European bioethical literature that may otherwise be difficult to find. Methods:, A collaboration model for pooling data from different centres was developed. The policy was to accomplish data uniformity, while still allowing for local differences in terms of software, indexing practices and resources. Records contributed to the database follow common standards in terms of data fields and indexing terms. The indexing terms derive from two thesauri, Thesaurus Ethics in the Life Sciences (TELS) and Medical Subject Headings (MeSH). Combining elements from search tools developed previously, the developers sought to find a technical solution optimized for this data model. An approach relying on a thesaurus database that is loaded along with the bibliographic database is described. Results and conclusions:, The present case study offers examples of possible approaches to several tasks often encountered in database development, such as: merging data from diverse sources, getting the most out of indexing terms used in a database, and handling more than one thesaurus in the same system. [source] Annual temperature history in Southwest Tibet during the last 400 years recorded by tree ringsINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 7 2010Bao Yang Abstract We present a tree ring-width record from the southern Tibetan Plateau (TP) which spans from 1612,1998 AD (387 years). The series was developed from Tibetan juniper (Juniperus tibetica Kom) growing at sites near the western distribution limit of the species. Two versions of the chronology, a traditionally standardized chronology (TSC) and a regional curve standardization (RCS)chronology were developed. Linear regression models between ring width and mean annual temperature account for 41% (TSC) and 43% (RCS) of the annual (July,June) temperature variance for the period 1957,1998. According to the TSC reconstruction, warm periods occurred during the 1620s, 1650,1675, 1720s, 1740,1790, 1810s, 1850s,1890s, 1935,1950, and 1957,1964 and since 1980. Cold conditions prevailed during the 1630s,1640s, 1680s,1710s, 1730s, 1820,1840s, 1900s,1920s and the 1970s. Within the last 400 years, the late-20th century warming is distinctive but still within the range of natural climatic variability of this region. Comparison of our TSC reconstruction with proxy temperature records from other parts of the TP shows that the cold conditions during the 1730s, 1900s,1920s, and 1970s, and the warm periods during the 1770,1800, 1850s,1890s, 1935,1950, and 1957,1964 and since 1980 were synchronously occurring broad-scale climate anomalies on the whole TP. Differences between the reconstructions are found during the 17th century and around 1760, which were probably caused by local differences in temperature change and different sensitivity in seasonality. The RCS series portrays low-frequency variations such as warm periods during 1620,1640, 1650,1690, 1715,1790, and 1845,1875, and cold conditions during 1640,1650, 1690,1715, and 1875,1995. These long-term trends need to be verified by developing other proxy records that target to capture low-frequency signals in the future. Copyright © 2009 Royal Meteorological Society [source] Very high resolution interpolated climate surfaces for global land areasINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 15 2005Robert J. Hijmans Abstract We developed interpolated climate surfaces for global land areas (excluding Antarctica) at a spatial resolution of 30 arc s (often referred to as 1-km spatial resolution). The climate elements considered were monthly precipitation and mean, minimum, and maximum temperature. Input data were gathered from a variety of sources and, where possible, were restricted to records from the 1950,2000 period. We used the thin-plate smoothing spline algorithm implemented in the ANUSPLIN package for interpolation, using latitude, longitude, and elevation as independent variables. We quantified uncertainty arising from the input data and the interpolation by mapping weather station density, elevation bias in the weather stations, and elevation variation within grid cells and through data partitioning and cross validation. Elevation bias tended to be negative (stations lower than expected) at high latitudes but positive in the tropics. Uncertainty is highest in mountainous and in poorly sampled areas. Data partitioning showed high uncertainty of the surfaces on isolated islands, e.g. in the Pacific. Aggregating the elevation and climate data to 10 arc min resolution showed an enormous variation within grid cells, illustrating the value of high-resolution surfaces. A comparison with an existing data set at 10 arc min resolution showed overall agreement, but with significant variation in some regions. A comparison with two high-resolution data sets for the United States also identified areas with large local differences, particularly in mountainous areas. Compared to previous global climatologies, ours has the following advantages: the data are at a higher spatial resolution (400 times greater or more); more weather station records were used; improved elevation data were used; and more information about spatial patterns of uncertainty in the data is available. Owing to the overall low density of available climate stations, our surfaces do not capture of all variation that may occur at a resolution of 1 km, particularly of precipitation in mountainous areas. In future work, such variation might be captured through knowledge-based methods and inclusion of additional co-variates, particularly layers obtained through remote sensing. Copyright © 2005 Royal Meteorological Society. [source] Gender, social capital and location: understanding the interactionsINTERNATIONAL JOURNAL OF SOCIAL WELFARE, Issue 2 2007Karen Healy In recent years, governments in many post-industrial nations have re-discovered ,community'. Social capital and neighbourhood renewal are key concepts underpinning policies aimed at building the capacities of communities to respond to the problems facing them. Despite the apparent consensus amongst Anglo-American governments about the use of social capital, and related concepts, as a guide for policy formulation these ideas remain hotly contested amongst social policy commentators. Against a general backdrop of disquiet about the application of social capital initiatives to communities facing significant social and economic challenges, some commentators highlight the curious inattention to gender in debates about social capital. This is all the more troubling given the apparent reliance of social capital initiatives on the informal network-building activities often undertaken by women. In this article we present findings from an empirical study of social capital creation across four geographically diverse communities. We show that the relationship between gender and social capital is complex. We argue that the ,one-size-fits-all' approach to social capital formulation fails to recognise gendered patterns of social capital formulation, but so, too, do analyses of gender that neglect the complex interactions between gender and other factors, particularly location, age and income. We argue for approaches to policy formulation that recognise local differences, including differences between and amongst men and women in social capital creation. [source] Long-standing environmental conditions, geographic isolation and host,symbiont specificity influence the relative ecological dominance and genetic diversification of coral endosymbionts in the genus SymbiodiniumJOURNAL OF BIOGEOGRAPHY, Issue 5 2010Todd C. LaJeunesse Abstract Aim, This study examines the importance of geographic proximity, host life history and regional and local differences in environment (temperature and water clarity) in driving the ecological and evolutionary processes underpinning the global patterns of diversity and distribution of symbiotic dinoflagellates. By comparing and contrasting coral,algal symbioses from isolated regions with differing environmental conditions, we may assess the potential of coral communities to respond to significant changes in climate. Location, Indian Ocean. Methods, Community assemblages of obligate symbiotic invertebrates were sampled at numerous sites from two regions, the north-eastern Indian Ocean (Andaman Sea, western Thailand) and the western Indian Ocean (Zanzibar, Tanzania). Molecular genetic methods, including denaturing gradient gel electrophoresis analysis of the ribosomal internal transcribed spacers, DNA sequencing and microsatellite genotyping, were used to characterize the ,species' diversity and evolutionary relationships of symbiotic dinoflagellates (genus Symbiodinium). Host,symbiont specificity, geographic isolation and local and regional environmental factors were evaluated in terms of their importance in governing the distribution and prevalence of certain symbiont taxa. Results, Host-generalist symbionts (C3u and D1-4, formerly D1a now designated Symbiodinium trenchi) frequently occurred alone and sometimes together in hosts with horizontal modes of symbiont acquisition. However, the majority of Symbiodinium diversity consisted of apparently host-specific ,species'. Clade C Symbiodinium were diverse and dominated host assemblages from sites sampled in the western Indian Ocean, a pattern analogous to symbiont communities on the Great Barrier Reef with similar environmental conditions. Clade D Symbiodinium were diverse and occurred frequently in hosts from the north-eastern Indian Ocean, especially at inshore locations, where temperatures are warmer, water turbidity is high and large tidal exchanges commonly expose coral populations to aerial desiccation. Main conclusions, Regional and local differences in cnidarian,algal combinations indicate that these symbioses are ecologically and evolutionarily responsive and can thrive under various environmental conditions. The high temperatures and turbid conditions of the north-eastern Indian Ocean partly explain the ecological success of Clade D Symbiodinium relative to Clade C. Phylogenetic, ecological and population genetic data further indicate that Clade D has undergone an adaptive radiation, especially in regions around Southeast Asia, during the Pleistocene. [source] Prediction of human pharmacokinetics,gut-wall metabolismJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 10 2007Urban Fagerholm Intestinal mucosal cells operate with different metabolic and transport activity, and not all of them are involved in drug absorption and metabolism. The fraction of these cells involved is dependent on the absorption characteristics of compounds and is difficult to predict (it is probably small). The cells also appear comparably impermeable. This shows a limited applicability of microsome intrinsic clearance (CLint)-data for prediction of gut-wall metabolism, and the difficulty to predict the gut-wall CL (CLGW) and extraction ratio (EGW). The objectives of this review were to evaluate determinants and methods for prediction of first-pass and systemic EGW and CLGW in man, and if required and possible, develop new simple prediction methodology. Animal gut-wall metabolism data do not appear reliable for scaling to man. In general, the systemic CLGW is low compared with the hepatic CL. For a moderately extracted CYP3A4-substrate with high permeability, midazolam, the gut-wall/hepatic CL-ratio is only 1/35. This suggests (as a general rule) that systemic CLGW can be neglected when predicting the total CL. First-pass EGW could be of importance, especially for substrates of CYP3A4 and conjugating enzymes. For several reasons, including those presented above and that blood flow based models are not applicable in the absorptive direction, it seems poorly predicted with available methodology. Prediction errors are large (several-fold on average; maximum-15-fold). A new simple first-pass EGW -prediction method that compensates for regional and local differences in absorption and metabolic activity has been developed. It has been based on human cell in-vitro CLint and fractional absorption from the small intestine for reference (including verapamil) and test substances, and in-vivo first-pass EGW -data for reference substances. First-pass EGW -values for CYP3A4-substrates with various degrees of gastrointestinal uptake and CLint and a CYP2D6-substrate were well-predicted (negligible errors). More high quality in-vitro CLint - and in-vivo EGW -data are required for further validation of the method. [source] FOG AND ACIDIFICATION IMPACTS ON ION BUDGETS OF BASINS IN NOVA SCOTIA, CANADA,JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 3 2000Shelagh Yanni ABSTRACT: We examined hydrogeochemical records for a dozen watersheds in and near Kejimkujik National Park in southwestern Nova Scotia by relating stream ion concentrations and fluxes to atmospheric deposition, stream type (lake inlet versus outlet; brown versus clear water), and watershed type (catchment area, topography, soils, and dominant forest cover type). We found that fog and dry deposition make important contributions to S, N, Cl, H, Ca, Mg, K, and Na inputs into these watersheds. Seasalt chloride deposition from rain, snow, fog, and dry deposition equal total stream outputs on a region-wide basis. Chloride outputs, however, differ among watersheds by a factor of about two, likely due to local differences in air flow and vegetational fog interception. We found that most of the incoming N is absorbed by the vegetation, as stream water NO3 - and NH4+ are very low. Our results also show that the vegetation and the soils absorb about half of the incoming SO42. In comparison with other North American watersheds with similar forest vegetation, Ca outputs are low, while Mg and K outputs are similar to other regions. Soil exchangeable Ca and soil cation exchange capacity are also very low. We found that first-order forest streams with no upstream lakes have a distinct seasonal pattern that neither corresponds with the seasonal pattern of atmospheric deposition, nor with the seasonal pattern of downstream lake outlets. [source] A 13C solid-state NMR analysis of steroid compoundsMAGNETIC RESONANCE IN CHEMISTRY, Issue 8 2008Jen-Hsien Yang Abstract 13C CP/MAS solid-state NMR spectroscopy has been utilized to analyze six steroid compounds, namely testosterone (Tes), hydrocortisone (Cor), trans -dehydroandrosterone (Adr), prednisolone (Prd), prednisone (Pre) and estradiol (Est). Among them, Tes displays a doublet pattern for all residues, whereas Prd, Pre and Est, exhibit exclusively singlets. For Cor and Adr, the 13C spectra contain both doublet and singlet patterns. The 13C doublet signal, with splittings of 0.2,1.5 ppm, are ascribed to local differences in the ring conformations associated with polymorphism. We have assigned all of the 13C resonances to the different residues in these steroid compounds on the basis of solution NMR data. The C-7, C-8, C-10, C-15 and C-16 residues of Tes, Cor and Adr consistently give rise to singlets or doublets with splittings of less than 0.5 ppm, indicating similar local conformations. Accompanying hydration and dehydration processes, a reversible phase transformation between ,- and ,-crystal forms has been observed in Tes, corresponding to singlet and doublet13C patterns, respectively. To further characterize the ring conformations in the ,-form, we have successfully extracted chemical shift tensor elements for the 13C doublets. It is demonstrated that 13C solid-state NMR spectroscopy provides a reliable and sensitive means of characterizing polymorphism in steroids. Copyright © 2008 John Wiley & Sons, Ltd. [source] BANKING MARKET STRUCTURE, CREATION AND ACTIVITY OF FIRMS: EARLY EVIDENCE FOR COOPERATIVES IN THE ITALIAN CASEANNALS OF PUBLIC AND COOPERATIVE ECONOMICS, Issue 4 2009Francesca Gagliardi ABSTRACT,:,This paper investigates whether local differences in banking competition impact on the creation and activity of firms, with a special focus on cooperatives. The empirical analysis, implemented on a sample of Italian firms, reveals non-monotonic effects of bank market power on firm creation and activity. In regard to the former, a bell-shaped relationship is found for both cooperative and non-cooperative firms, suggesting that a moderately concentrated banking market favours firms' creation. A less homogeneous pattern characterizes firms' activity: a bell-shaped parabola is still found for non-cooperative firms, while a U-shaped relationship emerges for cooperatives, showing that active coops benefit from relatively more intense banking competition. [source] Vegetation and climate history in the Westeifel Volcanic Field (Germany) during the past 11 000 years based on annually laminated lacustrine maar sedimentsBOREAS, Issue 4 2009THOMAS LITT Lakes Holzmaar and Meerfelder Maar are located in the Westeifel Volcanic Field less than 10 km apart. Both maar lakes are well known for their annually laminated (varved) sediments covering the past 15 000 years. Here, we focus on reconstructing the history of Holocene vegetation, human impact and climate using high-resolution pollen data. Detailed correlation between the two records using palynologically defined tie-points provides for the first time a test of the precision of the individual varve chronologies. The high-resolution pollen records of both Holzmaar and Meerfelder Maar show continuous natural successions of vegetation during the early and mid-Holocene controlled by the development of soil, climate, immigration and competitive expansion of thermophilous tree species. From 6500 varve yr BP onwards, human impact became an increasingly more important factor. Given the high chronological precision of both records, regional similarities, but also local differences of anthropogenically influenced vegetation changes, can be recognized. The reconstructed July temperature between 8500 and 5000 varve yr BP is around 1 °C higher than today, most likely in response to higher summer insolation related to orbital forcing. High variability of reconstructed July and January temperatures as well as annual precipitation around 5000 varve yr BP is a prominent climatic signal. This is consistent with other records in several areas of Europe which also identify this period as climatically highly variable. [source] | |||||||||||||||||||