			Home About us Contact

Lipid Supplements (lipid + supplement)

Distribution by Scientific Domains

Earth and Environmental Science	80%

Selected Abstracts

Efficacy of various lipid supplements in formulated pellet diets for juvenile Scylla serrata

AQUACULTURE RESEARCH, Issue 10 2010
Unniyampurath Unnikrishnan
Abstract Efficacy of sunflower oil (diet SF) and soybean oil (diet SB) alone and in combination with cod liver oil (diets M1-2.80:1.40:1.40, M2-2.80:2.24:0.56 and M3-2.80:0.56:2.24; cod liver oil:sunflower oil:soybean oil) as lipid supplements (5.6%) in formulated diets (crude fat ,9.79%) for juvenile Scylla serrata (weight=0.28±0.07 g, carapace width=9.7±0.1 mm) were compared with diet CL, containing cod liver oil alone as the lipid supplement (6 diets × 24 crabs stocked individually, randomized block design). Growth performance, nutrient (protein and lipid) intake and gain of crabs fed M1, M2 and M3 were higher (P,0.05) than the crabs fed SF and SB, but were not significantly different (P,0.05) from crabs fed CL. Dietary fatty acids (FAs) are found to influence the FA profile of test crabs. Higher tissue levels of 16:1n-7, 18:1n-9 and 18:1n-7 reflected the essential FA deficiency in crabs fed diets supplemented only with vegetable oils. Results confirmed that S. serrata could utilize vegetable oil supplements in the formulated diets as a partial replacement (50%) of cod liver oil without compromising growth and survival. Partial substitution of marine fish oil with suitable vegetable oils can reduce the feed cost considerably, in the context of rising fish oil prices. [source]

Influence of forage fish and dietary lipid supplements on egg quality and fry production in channel catfish (Ictalurus punctatus) × blue catfish (Ictalurus furcatus) hybridization

AQUACULTURE NUTRITION, Issue 2 2010
E.R. DURLAND
Abstract Hybrid catfish (channel catfish Ictalurus punctatus × blue catfish Ictalurus furcatus) display characteristics that are favourable to aquaculture production. Low hatch percentages are a principal reason this hybrid is not used widely in the catfish industry. This study was conducted to determine whether additional food source rich in lipids may lead to a higher quality egg production. A 10-week feed trial was conducted in ponds in Auburn, AL. A total of 219 female Kansas Select channel catfish were stocked into nine ponds, 0.04 ha in size. Three dietary treatments were randomly allocated to the ponds. Diet-1 was a standard 60 g kg,1 lipid floating catfish feed. Diet-2 was the same feed supplemented with forage fish at ,28 kg ha,1. The third diet was the aforementioned catfish feed topcoated with 20 g kg,1 lipid [10 g kg,1 menhaden fish oil, 5 g kg,1 high docosahexaenoic acid (DHA) oil and 5 g kg,1 high arachidonic acid oil]. Results indicate that brood fish fed the high lipid diet spawned larger egg masses and had larger eggs both in weight and in diameter, with increased complements of fatty acids such as DHA, eicosapentaenoic acid and total n-3 fatty acids. The neutral and polar lipid fractions are also presented. [source]

Efficacy of various lipid supplements in formulated pellet diets for juvenile Scylla serrata

Growth of NS0 Cells in Protein-Free, Chemically Defined Medium

BIOTECHNOLOGY PROGRESS, Issue 5 2000
Stephen Gorfien
Many hybridoma and recombinant myeloma cell lines have been successfully adapted to growth in protein-free media. Compared with serum-supplemented media, use of protein-free media promotes superior cell growth and protein expression and facilitates downstream purification of the expressed product. Owing to its sterol auxotrophy, the NS0 myeloma is normally grown in either a serum-supplemented medium or a serum-free medium supplemented with an animal-derived lipoprotein. CD Hybridoma Medium (a protein-free, chemically defined formulation) grows many cell lines that do not exhibit lipid dependence, but this medium does not support growth of NS0 cells without further lipid supplementation. We tested several commercially available lipid supplements in CD Hybridoma Medium, including bovine EX-CYTE VLE. None of the tested supplements supported long-term growth of NS0 cells in CD Hybridoma Medium. Sustained long-term growth of NS0 cells was achieved in CD Hybridoma Medium supplemented with various animal- or plant-derived lipids complexed with cyclodextrin. NS0 cells adapted to CD Hybridoma Medium supplemented with cyclodextrin-lipid complex reached peak cell densities that were more than double those observed in serum-supplemented medium and were cultured for more than 15 passages. These cultures were also successfully cryopreserved and recovered in this defined medium. Through the use of cyclodextrin-based additives to CD Hybridoma Medium, it is possible to solubilize significant quantities of sterols and other lipids and to maintain a protein-free, chemically defined cultivation environment for NS0 cells. The culture system can be kept entirely free of animal-derived components if the supplement is made with plant-derived or synthetic lipids. [source]