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Leghorn Hens (leghorn + hen)

Distribution by Scientific Domains
Chemistry80%

Kinds of Leghorn Hens

  • white leghorn hen
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    Selected Abstracts

    Isolation and characterization of antimicrobial proteins and peptide from chicken liver

    JOURNAL OF PEPTIDE SCIENCE, Issue 6 2007
    Guan-Hong Li
    Abstract Endogenous antimicrobial peptides and proteins are crucial components of the innate immune system and play an essential role in the defense against infection. Antimicrobial activity was detected in the acid extract of livers harvested from healthy adult White Leghorn hens, Gallus gallus. Two antimicrobial proteins and one antimicrobial polypeptide were isolated from the liver extract by cation-exchange and gel filtration chromatography, followed by two-step reverse-phase high-performance liquid chromatography (RP-HPLC). These antimicrobial components were identified as histones H2A and H2B.V, and histone H2B C -terminal fragment using peptide mass fingerprinting and partial sequencing by tandem nanoelectrospray mass spectrometry. The proteins and the peptide identified in the present study, which exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria, were thermostable and showed salt-resistant activity. The antimicrobial properties of histones and histone fragment in chicken provide further evidence that histones, in addition to their role in nucleosome formation, may play an important role in innate host defense against intracellular or extracellular microbe invasion in a wide range of animal species. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd. [source]

    Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens

    PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 7 2006
    San-Yuan Huang
    Abstract Serum is believed to harbor thousands of distinct proteins that are either actively secreted or leak from various blood cells or tissues. Exploring protein composition in serum may accelerate the discovery of novel protein biomarkers for specific economic traits in livestock species. This study analyzed serum protein composition to establish a 2-DE reference map, and monitored protein dynamics of single-comb White Leghorn hens at 8, 19 and 23,weeks after hatching. A total of 119,CBB-stained and 315,silver-stained serum protein spots were analyzed by MALDI-TOF,MS. Of these, 98,CBB-stained and 94,silver-stained protein spots were significantly matched to existing chicken proteins. The identified spots represented 30,distinctive proteins in the serum of laying hens. To compare protein expression during development, expression levels of 47,protein spots were quantified by relative spot volume with Melanie,3 software. Ten protein spots increased and 3,protein spots decreased as hen age increased. Previous research has suggested that some of these proteins play critical roles in egg production. The differentially expressed proteins with unknown identities will be valuable candidates for further explorations of their roles in egg production of laying hens. [source]

    Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens

    PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 7 2006
    San-Yuan Huang
    No abstracts. [source]

    Identification of quantitative trait loci associated with egg quality, egg production, and body weight in an F2 resource population of chickens,

    ANIMAL GENETICS, Issue 2 2006
    M. A. Schreiweis
    Summary Egg production and egg quality are complex sex-limited traits that may benefit from the implementation of marker-assisted selection. The primary objective of the current study was to identify quantitative trait loci (QTL) associated with egg traits, egg production, and body weight in a chicken resource population. Layer (White Leghorn hens) and broiler (Cobb-Cobb roosters) lines were crossed to generate an F2 population of 508 hens over seven hatches. Phenotypes for 29 traits (weekly body weight from hatch to 6 weeks, egg traits including egg, albumen, yolk, and shell weight, shell thickness, shell puncture score, percentage of shell, and egg shell colour at 35 and 55 weeks of age, as well as egg production between 16 and 55 weeks of age) were measured in hens of the resource population. Genotypes of 120 microsatellite markers on 28 autosomal groups were determined, and interval mapping was conducted to identify putative QTL. Eleven QTL tests representing two regions on chromosomes 2 and 4 surpassed the 5% genome-wise significance threshold. These QTL influenced egg colour, egg and albumen weight, percent shell, body weight, and egg production. The chromosome 4 QTL region is consistent with multiple QTL studies that define chromosome 4 as a critical region significantly associated with a variety of traits across multiple resource populations. An additional 64 QTL tests surpassed the 5% chromosome-wise significance threshold. [source]