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Another Step (another + step)
Selected AbstractsAnother step toward global convergenceJOURNAL OF CORPORATE ACCOUNTING & FINANCE, Issue 6 2008Kang Cheng The Financial Accounting Standards Board and the International Accounting Standards Board have worked together to develop a single set of accounting standards for business combinations. It will make things easier for corporate finance and accounting professionals when dealing with domestic versus international acquisitions. However, at first glance, the new FASB standard looks like a total revision of SFAS No. 141. What are the latest changes? © 2008 Wiley Periodicals, Inc. [source] Making good research useful: Another step on the journeyAUSTRALIAN JOURNAL OF RURAL HEALTH, Issue 2 2009David Perkins No abstract is available for this article. [source] The paradox of the alcohol-paradox , another step towards the resolution of the ,alcohol energy wastage' controversyEUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 9 2000Suter No abstract is available for this article. [source] Another vaccine, another step forward?JOURNAL OF PAEDIATRICS AND CHILD HEALTH, Issue 11 2005K Grimwood No abstract is available for this article. [source] Investigation into the protein composition of human tear fluid using centrifugal filters and drop coating deposition Raman spectroscopyJOURNAL OF RAMAN SPECTROSCOPY, Issue 2 2009Jacob Filik Abstract Drop coating deposition Raman spectroscopy (DCDRS) is a simple method of analysing weak protein solutions. This study is another step in evaluating the analysis of tear fluid by DCDRS as a future medical diagnostic technique. The main aims of this study are to determine whether the DCDR spectra from tear samples contain signals from more than one protein (so relative levels can be measured) and, if so, are the proteins homogeneously distributed in the dried ring of the deposited material. Tear samples were collected from four healthy volunteers and pooled prior to analysis. Proteins were separated by mass into three groups using centrifugal filters. These groups contained proteins with (1) masses greater than 100 kDa, (2) masses between 100 and 50 kDa and (3) masses between 50 and 3 kDa. DCDR spectra from each of these protein group solutions displayed significant differences, confirming that the mass separation had been successful. When used as basis vectors for least-squares fitting, these spectra (and that of urea) produced excellent fits to the normal tear spectra. Least-squares fitting of spectra from the same point on a single sample and from several drops of the same sample showed that the tear DCDR spectra were highly reproducible. Raman point mapping of the tear ring showed significant radial ring variation, especially towards the outer edge of the ring. The specific peak changes in the protein signal across the ring suggested that the difference in the outer edge was due to protein desiccation as opposed to inhomogeneous protein deposition. Copyright © 2008 John Wiley & Sons, Ltd. [source] Polar body biopsy and aneuploidy testing by simultaneous detection of six chromosomesPRENATAL DIAGNOSIS, Issue 10 2005Markus Montag Abstract Objectives To simultaneously detect six chromosomes in a single round of fluorescence in situ hybridization (FISH) during polar body diagnosis and aneuploidy testing in human in vitro fertilization (IVF) treatment. Methods A commercially available five-color FISH probe was modified by an additional chromosome probe. This kit was first tested on lymphocyte spreads and then used for polar body diagnosis (PBD) in patients with advanced maternal age and repeated implantation failure. The outcome of IVF treatment was compared with a control group. Results All six chromosomes could be simultaneously detected and easily distinguished by FISH analysis. PBD and aneuploidy testing were performed in 75 treatment cycles and compared with 126 controls. The biochemical pregnancy rate was significantly higher in the PBD group (37.1% vs 22.9%, p < 0.05) and a trend was observed for higher clinical pregnancy and implantation rates (24.22% and 14.4% vs 18.62% and 10.8%, respectively) and lower abortion rates (20% vs 31.8%) following PBD. Conclusions The simultaneous detection of six chromosomes in a single FISH round is possible and can be applied to PBD. This approach may present another step towards increasing the number of chromosomes for aneuploidy testing. Copyright © 2005 John Wiley & Sons, Ltd. [source] Identification of transcripts modulated by ETV6 expressionBRITISH JOURNAL OF HAEMATOLOGY, Issue 1 2007Gino Boily Summary Deletions at chromosome 12p12-13 are observed in 26,47% of childhood pre-B acute lymphoblastic leukaemia (ALL) cases, suggesting the presence of a tumour suppressor gene (TSG). Accumulating genetic and functional evidence points to ETV6 as being the most probable TSG targeted by the deletions. ETV6 is a ubiquitously expressed transcription factor of the ETS family with very few known targets. To understand its function and to elucidate the impact of its absence in leukaemia, we conducted a study to identify targeted genes. Following the induction of ETV6 expression, global expression was evaluated at different time points. We identified 87 modulated genes, of which 10 (AKR1C1, AKR1C3, IL18, LUM, PHLDA1, PTGER4, PTGS2, SPHK1, TP53 and VEGF) were validated by real-time quantitative reverse transcription-polymerase chain reaction. To assess the significance of the validated candidate genes in leukaemia, their expression patterns were determined, as well as that of ETV6, in pre-B ALL patients. The expression of IL18, LUM, PTGER4, SPHK1 and TP53 was significantly correlated with that of ETV6, further suggesting that ETV6 could regulate the expression of these genes in leukaemia. This work constitutes another step towards the understanding of the functions of ETV6 and the impact of its inactivation in childhood leukaemia. [source] | ||||||||||