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Isolated Only (isolated + only)
Selected AbstractsCentral nervous system is a sanctuary site for chronic myelogenous leukaemia treated with imatinib mesylateINTERNAL MEDICINE JOURNAL, Issue 6 2009Y. Isobe Abstract Imatinib mesylate (IM) is currently used as the first therapeutic choice against chronic myelogenous leukaemia (CML). Because IM poorly penetrates the blood-brain barrier, IM-treated CML patients may have a potential risk of central nervous system (CNS) involvement. Here we report a case with lymphoid blast crisis isolated only in CNS after bacterial meningitis, although the patient achieved and maintained complete cytogenetic response by IM therapy. It is important to consider isolated CNS blast crisis as a possible event in IM-treated CML patients. [source] Transmission of cotton seed and boll rotting bacteria by the southern green stink bug (Nezara viridula L.)JOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2007E.G. Medrano Abstract Aims:, To determine the ability of the southern green stink bug (SGSB) (Nezara viridula L.) to transmit Pantoea agglomerans into cotton (Gossypium hirsutum) bolls. Methods and Results:, An SGSB laboratory colony was kept on fresh green beans. A P. agglomerans variant resistant to rifampicin (Rif) (strain Sc 1-R) was used as the opportunistic cotton pathogen. Adult insects were individually provided green beans that were sterilized and then soaked in either sterile water or in a suspension of strain Sc 1-R. Insects were individually caged with an unopened greenhouse-grown cotton boll. After 2 days, live SGSB were collected, surfaced sterilized, ground, serially diluted, and then plated on nonselective media and media amended with Rif. Exterior and interior evidence of feeding on bolls was recorded 2 weeks after exposure to insects. Seed and lint tissue were harvested, ground, serially diluted, and then plated on media with and without Rif. Bacteria were recovered on nonselective media from all insects, and from seed and lint with signs of insect feeding at concentrations ranging from 102 to 109 CFU g,1 tissue. The Sc 1-R strain was isolated only from insects exposed to the marked strain and from seed and lint of respective bolls showing signs of insect feeding. Evidence of insect feeding on the exterior wall of the carpel was not always apparent (47%), whereas feeding was always observed (100%) on the interior wall in association with bacterial infections of seed and lint. Conclusions:,Nezara viridula readily ingested the opportunistic P. agglomerans strain Sc 1-R and transmitted it into unopened cotton bolls. Infections by the transmitted Sc 1-R strain caused rotting of the entire locule that masked internal carpel wounds incurred by insect feeding. Bacteria were recovered from penetration points by insects not exposed to the pathogen, but locule damage was limited to the area surrounding the feeding site (c. 3 mm). Significance and Impact of the Study:, This is the first study that demonstrates the ability of SGSB to acquire and transmit plant pathogenic bacteria into cotton bolls. [source] A comparison of two pre-enrichment media prior to immunomagnetic separation for the isolation of E. coli O157 from bovine faecesJOURNAL OF APPLIED MICROBIOLOGY, Issue 1 2003G. Foster Abstract Aims: To compare the sensitivity of two pre-enrichment broth media prior to immunomagnetic separation for the isolation of Escherichia coli O157 from cattle faeces. Methods and Results: One-gram portions of 721 cattle faeces collected from 43 farms were pre-enriched in buffered peptone water containing vancomycin, cefixime and cefsulodin (BPW-VCC) and buffered peptone water without additives (BPW-WOA), respectively. A total of 137 samples were positive for E. coli O157: 127 pre-enriched with BPW-WOA and 89 pre-enriched in BPW-VCC. Representative isolates were tested for phage type, verotoxin and eae (E. coli attaching and effacing) gene sequences, resulting in the recognition of eight different types. All the E. coli O157 types recognized were isolated by both methods except for three different strains, each of which were isolated only on a single occasion: two by BPW-WOA and another by BPW-VCC. Conclusions: The results clearly demonstrate, under the conditions of this study, that BPW without antibiotics was the superior pre-enrichment medium for the isolation of E. coli O157 from cattle faeces. Significance and Impact of the Study: The use of BPW-WOA in preference to BPW-VCC for the isolation of E. coli O157 from cattle faeces in future research and outbreak studies should lead to a higher number of positive isolates. [source] Cefixime,tellurite rhamnose MacConkey agar for isolation of Vero cytotoxin-producing Escherichia coli serogroup O26 from Scottish cattle and sheep faecesLETTERS IN APPLIED MICROBIOLOGY, Issue 3 2008J. Evans Abstract Aims:, To compare rhamnose MacConkey agar supplemented with cefixime and tellurite (CT-RMac) and tryptone bile X-glucuronide (TBX) agars as isolation media for Vero cytotoxin-producing Escherichia coli (VTEC) serogroup O26 from animal faeces. Methods and Results:, Nine VTEC O26 were isolated from sheep faeces; out of which six were isolated only on CT-RMac and one was isolated only on TBX. One hundred and twelve VTEC O26 were isolated from calf faeces; out of which 97% were from CT-RMac and 52% were from TBX. In a study of E. coli O26 strains, 84% of VT-positive O26 did not ferment rhamnose when compared with 16% of VT-negative O26. VT-positive (19%) and VT-negative (39%) E. coli O26 strains did not grow on CT-RMac agar. Conclusions:, It is important to consider that VTEC O26 strains either may ferment rhamnose or may be sensitive to the CT supplement of CT-RMac agar. Significance and Impact of the Study:, This work compares CT-RMac and TBX agars as isolation medium for VTEC O26 from Scottish animal faeces and highlights that VTEC O26 may be missed if only CT-RMac agar is used. [source] Identification of genes encoding N -glycan processing ,- N -acetylglucosaminidases in Trichoplusia ni and Bombyx mori: Implications for glycoengineering of baculovirus expression systemsBIOTECHNOLOGY PROGRESS, Issue 1 2010Christoph Geisler Abstract Glycoproteins produced by non-engineered insects or insect cell lines characteristically bear truncated, paucimannose N -glycans in place of the complex N -glycans produced by mammalian cells. A key reason for this difference is the presence of a highly specific N -glycan processing ,- N -acetylglucosaminidase in insect, but not in mammalian systems. Thus, reducing or abolishing this enzyme could enhance the ability of glycoengineered insects or insect cell lines to produce complex N -glycans. Of the three insect species routinely used for recombinant glycoprotein production, the processing ,- N -acetylglucosaminidase gene has been isolated only from Spodoptera frugiperda. Thus, the purpose of this study was to isolate and characterize the genes encoding this important processing enzyme from the other two species, Bombyx mori and Trichoplusia ni. Bioinformatic analyses of putative processing ,- N -acetylglucosaminidase genes isolated from these two species indicated that each encoded a product that was, indeed, more similar to processing ,- N -acetylglucosaminidases than degradative or chitinolytic ,- N -acetylglucosaminidases. In addition, over-expression of each of these genes induced an enzyme activity with the substrate specificity characteristic of processing, but not degradative or chitinolytic enzymes. Together, these results demonstrated that the processing ,- N -acetylglucosaminidase genes had been successfully isolated from Trichoplusia ni and Bombyx mori. The identification of these genes has the potential to facilitate further glycoengineering of baculovirus-insect cell expression systems for the production of glycosylated proteins. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] Synthesis and Characterisation of ,3 -Octahedral [Ni36Pd8(CO)48]6, and [Ni35Pt9(CO)48]6, Clusters Displaying Unexpected Surface Segregation of Pt Atoms and Molecular and/or Crystal Substitutional Ni/Pd and Ni/Pt DisorderCHEMISTRY - A EUROPEAN JOURNAL, Issue 9 2004Cristina Femoni Dr. Abstract The synthesis and structure, as well as the chemical and electrochemical characterisation of two new ,3 -octahedral bimetallic clusters with the general [Ni44,xMx(CO)48]6, (M = Pd, x = 8; M = Pt, x = 9) formula is reported. The [Ni35Pt9(CO)48]6, cluster was obtained in reasonable yields (56,% based on Pt) by reaction of [Ni6(CO)12]2, with 1.1 equivalents of PtII complexes, in ethyl acetate or THF as the solvent. The [Ni36Pd8(CO)48]6, cluster was obtained from the related reaction with PdII salts in THF, and was isolated only in low yields (5,10,% based on Pd), mainly because of insufficient differential solubility of its salts. The unit cell of the [NBu4]6[Ni35Pt9(CO)48] salt contains a substitutionally Ni,Pt disordered [Ni24(Ni14,xPtx)Pt6(CO)48]6, (x = 3) hexaanion. A combination of crystal and molecular disorder is necessary to explain the disordering observed for the Ni/Pt sites. The unit cell of the corresponding [Ni36Pd8(CO)48]6, salt contains two independent [Ni30(Ni8,xPdx)Pd6(CO)48]6, (x = 2) hexaanions. The two display similar substitutional Ni,Pd disorder, which probably arises only from crystal disorder. The structure of [Ni36Pd8(CO)48]6, establishes the first similarity between the chemistry of Ni-Pd and Ni-Pt carbonyl clusters. A comparison of the chemical and electrochemical properties of [Ni35Pt9(CO)48]6, with those of the related [Ni38Pt6(CO)48]6, cluster shows that surface colouring of the latter with Pt atoms decreases redox as well as protonation propensity of the cluster. In contrast, substitution of all internal Pt and two surface Ni with Pd atoms preserves the protonation behaviour and is only detrimental with respect to its redox aptitude. A qualitative rationalisation of the different surface-site selectivity of Pt and Pd, based on distinctive interplays of MM and MCO bond energies, is suggested. [source] | ||||||||||