Home About us Contact
|
Home About us Contact | ||
|
|
||
Intensity Decrease (intensity + decrease)
Selected AbstractsExpression of RNAs encoding for , and , integrin subunits in periodontitis and in cyclosporin A gingival overgrowthJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 11 2003A.-L. Bolcato-Bellemin Abstract Background: Variation of integrin expression in healthy and diseased gingiva revealed a potential biological role for these cell matrix receptors during gingival remodeling. Aim: Here we determined the level of RNA and tissue localization of different integrin subunits in periodontitis and cyclosporin A-induced gingival overgrowth. Methods: The level of expression was determined by Reverse Transcriptase Polymerase Chain Reaction in 12 periodontitis-affected patients, four patients exhibiting severe cyclosporin A-induced gingival overgrowth and seven healthy patients as controls. Results: The RNA encoding for ,1, ,2 and ,5 integrin subunits were reduced in periodontitis gingiva. The reduction observed was stronger in cyclosporin A-treated patients as compared to the healthy controls, while RNA encoding for ,1 subunit was increased. The RNA encoding for ,6 integrin was only reduced in cyclosporin A-treated gingiva. Immunohistochemistry showed that i) integrin ,2 expression is restricted to the gingival epithelium of cyclosporin A-treated patients, ii) the reduction of ,6 integrin expression in cyclosporin A-treated gingiva is due to loss of expression at focal contacts and iii) ,1 integrin is evenly distributed in the three populations with an intensity decrease in periodontitis and cyclosporin A-treated gingiva. Conclusion: Taken together these results showed a role for the integrin receptors in periodontal diseases and cyclosporin A-induced gingival overgrowth. Zusammenfassung Die Variation der Integrin Expression bei gesunder und erkrankter Gingiva zeigt eine potentielle biologische Rolle für diese Zellmatrixrezeptoren während der gingivalen Erneuerung. Wir bestimmten hier die Level von RNA und die Gewebelokalisation von unterschiedlichen Integrin Untereinheiten bei Parodontitis und Cyclosporin A induzierter gingivaler Wucherung. Die Level der Expression wurden mit der reversen Transscriptase Polymerase Kettenreaktion bei 12 Parodontitis-Patienten, 4 Patienten mit schwerer Cyclosporin A induzierter gingivaler Wucherung und sieben gesunden Kontrollpatienten bestimmt. Die kodierende RNA für ,1, ,2 und ,5 Integrin Untereinheiten waren in der Gingiva mit Parodontitis reduziert. Die beobachtete Reduktion war stärker bei den mit Cyclosporin A behandelten Patienten verglichen mit den gesunden Kontrollen, während kodierende RNA für ,1 Untereinheiten erhöht war. Die kodierende RNA für ,6 Integrin war nur bei der Cyclosporin A behandelten Gingiva reduziert. Die Immunhistochemie zeigte (i) die Integrin ,2 Expression ist auf das gingivale Epithel von Cyclosporin A behandelten Patienten beschränkt, (ii) die Reduktion von ,6 Integrin Expression bei Cyclosporin A behandelter Gingiva ist die Folge von fokalen Expressionverlusten und (iii) ,1 Integrin ist gleichmäßig verteilt in den drei Populationen mit einer Intensitätsabnahme bei Parodontitis und Cyclosporin A behandelter Gingiva. Zusammenfassend zeigen die Ergebnisse eine Rolle für die Integrin Rezeptoren bei den parodontalen Erkrankungen und Cyclosporin A induzierter gingivalen Wucherung. Résumé La variation d'expression des intégrines dans les tissus gingivaux sain et pathologique a démontré le rôle biologique potentiel de ces récepteurs de la matrice extracellulaire au cours du remodelage tissulaire gingival. La quantité d'ARN et la localisation tissulaire de certaines sous-unités d'intégrines dans la parodontite et l'hyperplasie gingivale induite par la ciclosporine A ont été déterminées. Le niveau d'expression a étéévalué par transcription inverse des ARN et réaction de polymérisation en chaine chez douze patients atteints de parodontite, quatre patients présentant une hyperplasie gingivale sévère induite par la ciclosporine A et sept patients sains ayant servi de témoins. L'expression des ARN codant pour les sous-unités ,1, ,2 et ,5était diminuée dans le tissu gingival atteint de parodontite. La diminution observée était plus importante chez les patients traités par la ciclosporine A, comparée aux témoins sains alors que l'expression de l'ARN codant pour la sous-unité,1était augmentée. L'expression de l'ARN codant pour la sous-unité,6était diminuée uniquement dans le tissu gingival traité par la ciclosporine A. L'immohistochimie a montré que (1) l'expression de la sous-unité,2 est limitée à l'épithélium gingival des patients traités par la ciclosporine A, (2) la diminution de l'expression de la sous-unité,6 dans le tissu gingival traité par la ciclosporine A est due à une perte des contacts focaux et (3) la sous-unité,1 est répartie de manière uniforme dans les trois groupes avec une diminution de l'intensité dans les cas de parodontite et d'hyperplasie gingivale induite par la ciclosporine A. Ces résultats montrent un rôle des récepteurs de type intégrine dans la pathologie parodontale et l'hyperplasie gingivale induite par la ciclosporine A. [source] Phonon properties of nanosized bismuth layered ferroelectric material,Bi2WO6JOURNAL OF RAMAN SPECTROSCOPY, Issue 9 2010M. Ma Abstract Nanosized Bi2WO6 was synthesized by a mild hydrothermal crystallization process. This method allowed obtaining plate-like crystallites of very small thickness (down to 3 nm). The effect of particle size on the structure and properties of Bi2WO6 was studied by X-ray diffraction (XRD), transmission electron microscopy, and Raman and infrared spectroscopies. It has been shown that the orthorhombic distortion decreases with decreasing particle size, but the structure of the smallest crystallites is still orthorhombic. Raman studies have also revealed a very strong intensity decrease for those modes that appear mainly for incident and scattered light polarized perpendicular to the layers. This behavior has been attributed to a decrease in the orthorhombic distortion and a plate-like shape of the nanocrystallites. Copyright © 2009 John Wiley & Sons, Ltd. [source] Photoinduced electron transfer in glucose oxidase: a picosecond time-resolved ultraviolet resonance Raman studyJOURNAL OF RAMAN SPECTROSCOPY, Issue 11 2008Akiko Fujiwara Abstract Picosecond time-resolved ultraviolet resonance Raman (UVRR) spectroscopy has been applied to photoinduced electron transfer (ET) of glucose oxidase (GOD). In this study, we succeeded in directly observing changes in the aromatic amino acid residues in the photoinduced ET of GOD for the first time. UVRR spectra excited at 226 nm showed bands from Trp and Tyr residues. An intensity decrease of the Trp UVRR bands and the appearance of the UVRR bands attributable to Trp,+ were observed in the time-resolved spectra. In the time-resolved UVRR spectra excited at 240 nm, the intensity decrease of the flavin adenine dinucleotide (FAD) bands was also observed on the same time scale. These results showed that the Trp residue(s) serves as an electron donor to excited-state FAD in the photoinduced ET of GOD. The comparison of the temporal changes of the Trp and FAD band intensities suggested that the ET from the Trp residue(s) to the FAD occurs with a time constant of ,1.5 ps. Copyright © 2008 John Wiley & Sons, Ltd. [source] Synthesis, characterization and ESR measurements of CoNiO nanoparticlesPHYSICA STATUS SOLIDI (B) BASIC SOLID STATE PHYSICS, Issue 8 2005Y. Köseo Abstract Powders of cobalt core with a nickel (II) oxide shell (CoNiO) have been studied by Electron Spin Resonance (ESR) technique in the temperature range of 10,300 K. A strong and broad (1300 G) single ESR peak in X-, K- and Q-band spectra has been observed at all the temperature ranges. While the resonance field remains almost constant, both the ESR line intensity and the line width were seen to increase first slowly down to Tc = 160 K. And then both the resonance field and the ESR signal intensity decrease and the line width increase with decreasing temperature. Below Tc the intensity smoothly decrease down to 10 K. This kind of behavior is attributed to a typical spin-glass like behavior. Some small and relatively smooth changes at about 60 K, 90 K, 210 K, and 250 K are appeared as well. A linear dependence of resonance field to microwave frequency is observed at room temperatures and the effective g-value and internal field are theoretically found as 2,17 and 90 G, respectively. The experimental data indicate a very strong spin disorder (spin frustration) due to antiferromagnetic exchange interactions among the spins. This is attributed to the D-M anisotropy on the particle surfaces that it is expected to enhance due to increment of surface-to-volume ratio. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] The rate of transport through a phosphate translocator affects delayed luminescence induction: an experiment and a theoretical modelANNALS OF APPLIED BIOLOGY, Issue 1 2001S KHUZNETSOVA Summary Delayed luminescence (DL) induction curves were studied in leaves from a mutant pea line containing mutations at both the r and rb loci, compared with leaves from wild type plants. Genes at the r and rb loci encode starch branching enzyme and ADP - glucose pyrophosphorylase, respectively. The presence of mutations at both loci, previously known to reduce the starch content in the dry mature seed by 75%, have been shown to lower the starch level in leaves by at least 20%. During induction, the half-time for the DL intensity decrease from maximum to steady state in the mutant was 1.5 ± 0.2 times longer than for the wild type. It is proposed that the prolongation of the induction period in leaves from the mutant plants is caused by a lack of inorganic phosphate (Pi) restricting the rate of ATP synthesis at the beginning of induction. The reduced Pi would be compensated by triose flow from the chloroplast, via the triose phosphate translocator, being exchanged for Pi from the cytosol. Analysis of our theoretical photosynthesis model confirmed that a decrease in the rate of Pi released from the Calvin cycle could lead to a prolongation of the induction period. [source] Side-chain supramolecular polymers with induced supramolecular chirality through H-bonding interactionsJOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 16 2008Francisco Vera Abstract Side-chain supramolecular polymers that show columnar mesomorphism have been prepared through H-bonding interactions between a polyvinylpyridine polymer as H-acceptor and different H-donors derived from benzoic acid. These compounds have been designed according to a promesogenic structure, that is, either disk-like or banana-like, to promote stacking and therefore the formation of columnar arrangements. IR studies confirmed the formation of H-bonds and demonstrated that the H-bond intensity decreases upon increasing temperature. The mesophase organizations were studied by polarized optical microscopy, differential scanning calorimetry, and X-ray diffraction. Associations containing poly-3-methyl-4-vinylpyridine showed supramolecular optical activity, as evidenced by circular dichroism studies on thin films. It is proposed that these supramolecular polymers adopt a helical structure that can be biased toward a given handedness by virtue of the configuration of the stereogenic centers in the peripheral tails of the acids. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 5528,5541, 2008 [source] Heating rate effects on the TL characteristics of hot filament CVD diamond filmPHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 9 2010E. Cruz-Zaragoza Abstract The thermoluminescence (TL) glow curve of polycrystalline chemically vapor deposited (CVD) diamond is strongly dependent on the heating rate used. In the present work, we report results about the heating rate effects on a 500,µm hot filament (HF) CVD diamond film grown on silicon substrate using a precursor gas mixture composition of CH4(5,sccm),CO(10,sccm),H2(400,sccm). The irradiations were performed with 60Co source. The TL measurements were carried out at different heating rates in the 2,30,K/s range. As the heating rate increases, the maximum TL peak intensity decreases and shifts to higher temperature. The shift was from 578 to 669,K for heating rates of 2 and 30,K/s, respectively. The kinetics parameter and its dependence on the heating rate were evaluated using the Chen's peak shape method and a computerized glow curve deconvolution (CGCD) method. [source] Analyzing experiments with degradation data for improving reliability and for achieving robust reliabilityQUALITY AND RELIABILITY ENGINEERING INTERNATIONAL, Issue 5 2001Chih-Hua Chiao Abstract Statistically designed experiments provide a proactive means for improving reliability; moreover, they can be used to design products that are robust to noise factors which are hard or impossible to control. Traditionally, failure-time data have been collected; for high-reliability products, it is unlikely that failures will occur in a reasonable testing period, so the experiment will be uninformative. An alternative, however, is to collect degradation data. Take, for example, fluorescent lamps whose light intensity decreases over time. Observation of light-intensity degradation paths, given that they are smooth, provides information about the reliability of the lamp, and does not require the lamps to fail. This paper considers experiments with such data for ,reliability improvement', as well as for ,robust reliability achievement' using Taguchi's robust design paradigm. A two-stage maximum-likelihood analysis based on a nonlinear random-effects model is proposed and illustrated with data from two experiments. One experiment considers the reliability improvement of fluorescent lamps. The other experiment focuses on robust reliability improvement of light-emitting diodes. Copyright © 2001 John Wiley & Sons, Ltd. [source] | ||||||||||