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Integrated Understanding (integrate + understanding)
Selected AbstractsSocial Identity, Organizational Identity and Corporate Identity: Towards an Integrated Understanding of Processes, Patternings and ProductsBRITISH JOURNAL OF MANAGEMENT, Issue 2007Joep P. Cornelissen This paper provides an overview of previous work that has explored issues of social, organizational and corporate identity. Differences in the form and focus of research into these three topics are noted. Social identity work generally examines issues of cognitive process and structure; organizational identity research tends to address the patterning of shared meanings; studies of corporate identity tend to focus on products that communicate a specific image. Nonetheless, across these areas there is general consensus that collective identities are (a) made viable by their positivity and distinctiveness, (b) fluid, (c) a basis for shared perceptions and action, (d) strategically created and managed, (e) qualitatively different from individual identities and (f) the basis for material outcomes and products. This paper calls for greater cross-fertilization of the three identity literatures and discusses requirements for the integration of micro- and macro-level analyses. [source] Single-cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its "index sorting" function for stem cell researchDEVELOPMENT GROWTH & DIFFERENTIATION, Issue 1 2010Tetsutaro Hayashi To achieve an integrated understanding of the stem cell system of planarians at both the cellular and molecular levels, we developed a new method by combining "fluorescent activated cell sorting (FACS) index sorting" analysis and single-cell reverse transcription,polymerase chain reaction (RT,PCR) to detect the gene expression and cell cycle state of stem cells simultaneously. Single cells were collected using FACS, and cDNAs of each cell were used for semi-quantitative RT,PCR. The results were plotted on the FACS sorting profile using the "index sorting" function, which enabled us to analyze the gene expression in combination with cell biological data (such as cell cycle phase) for each cell. Here we investigated the adult stem cells of planarians using this method and obtained findings suggesting that the stem cells might undergo commitment during S to G2/M phase. This method could be a powerful and straightforward tool for examining the stem cell biology of not only planarians but also other organisms, including vertebrates. [source] Integrated regulation in response to aromatic compounds: from signal sensing to attractive behaviourENVIRONMENTAL MICROBIOLOGY, Issue 12 2003Victoria Shingler Summary Deciphering the complex interconnecting bacterial responses to the presence of aromatic compounds is required to gain an integrated understanding of how aromatic catabolic processes function in relation to their genome and environmental context. In addition to the properties of the catabolic enzymes themselves, regulatory responses on at least three different levels are important. At a primary level, aromatic compounds control the activity of specific members of many families of transcriptional regulators to direct the expression of the specialized enzymes for their own catabolism. At a second level, dominant global regulation in response to environmental and physiological cues is incorporated to subvert and couple transcription levels to the energy status of the bacteria. Mediators of these global regulatory responses include the alarmone (p)ppGpp, the DNA-bending protein IHF and less well-defined systems that probably sense the energy status through the activity of the electron transport chain. At a third level, aromatic compounds can also impact on catabolic performance by provoking behavioural responses that allow the bacteria to seek out aromatic growth substrates in their environment. [source] Linking microbial activity and soil organic matter transformations in forest soils under elevated CO2GLOBAL CHANGE BIOLOGY, Issue 2 2005S. A. Billings Abstract Soil organic matter (SOM) dynamics ultimately govern the ability of soil to provide long-term C sequestration and the nutrients required for ecosystem productivity. Predicting belowground responses to elevated CO2 requires an integrated understanding of SOM transformations and the microbial activity that governs them. It remains unclear how the microorganisms upon which these transformations depend will function in an elevated CO2 world. This study examines SOM transformations and microbial metabolism in soils from the Duke Free Air Carbon Enrichment site in North Carolina, USA. We assessed microbial respiration and net nitrogen (N) mineralization in soils with and without elevated CO2 exposure during a 100-day incubation. We also traced the depleted C isotopic signature of the supplemental CO2 into SOM and the soils' phospholipid fatty acids (PLFA), which serve as biomarkers for living cells. Cumulative net N mineralization in elevated CO2 soils was 50% that in control soils after a 100-day incubation. Respiration was not altered with elevated CO2. C : N ratios of bulk SOM did not change with elevated CO2, but incubation data suggest that the C : N ratios of mineralized organic matter increased with elevated CO2. Values of SOM ,13C were depleted with elevated CO2 (,26.7±0.2 vs. ,30.2±0.3,), reflecting the depleted signature of the supplemental CO2. We compared ,13C of individual PLFA with the ,13C of SOM to discern incorporation of the depleted C isotopic signature into soil microbial groups in elevated CO2 plots. PLFA i15:0, a15:0, and 10Met18:0 reflected significant incorporation of recently produced photosynthate, suggesting that the bacterial groups defined by these biomarkers are active metabolizers in elevated CO2 soils. At least one of these groups (actinomycetes, 10Met18:0) specializes in metabolizing less labile substrates. Because control plots did not receive an equivalent 13C tracer, we cannot determine from these data whether this group of organisms was stimulated by elevated CO2 compared with these organisms in control soils. Stimulation of this group, if it occurred in the elevated CO2 plot, would be consistent with a decline in the availability of mineralizable organic matter with elevated CO2, which incubation data suggest may be the case in these soils. [source] Living with uncertainty: concept advancementJOURNAL OF ADVANCED NURSING, Issue 6 2007Janice Penrod Abstract Aim., This paper reports a study to demonstrate how the scientific understanding of the concept of uncertainty was advanced through a phenomenological study of living with uncertainty. Background., Techniques for concept analysis have evolved to subsume strategies for advancing a concept towards greater clarity and utility for research and practice. Recently, it has been argued that a clear delineation of techniques for concept analysis as separate and distinct from techniques of concept advancement is warranted. This article applies such delineated processes to demonstrate the advancement of the concept of uncertainty. Method., Concept analysis was used to establish an integrated understanding of the state of the science. Gaps in understanding were carefully analysed, resulting in the research question guiding the next phase of concept advancement: what is the nature of the lived experience of uncertainty? A phenomenological investigation of the experience of uncertainty among family caregivers was conducted. Then, using methods of template comparison, the conceptual attributes identified through the phenomenological study were compared and contrasted with the theoretical definition derived through concept analysis. Finally, a new conceptual definition of higher order abstraction, with greater pragmatic utility, was derived. Findings., Uncertainty is rooted in the individual's perception of outcomes or meaning of a situation. Such perceptions challenge one's sense of confidence and/or control to yield varied types and modes of uncertainty. Uncertainty is present oriented. Both cognitive and precognitive ways of knowing are influential in ascribing meaning, anticipating outcomes and adapting strategies. Conclusions., One's sense of confidence and sense of control are primary essences that determine the nature of the experience of uncertainty. The experience of living with uncertainty is dynamic, with fluctuations in the types and modes of uncertainty in response to precognitive and cognitive ways of knowing. Probabilistic paradigms preclude existential and situational modes of uncertainty for which probabilities cannot be appreciated. [source] Origins and Applications of London Dispersion Forces and Hamaker Constants in CeramicsJOURNAL OF THE AMERICAN CERAMIC SOCIETY, Issue 9 2000Roger H. French The London dispersion forces, along with the Debye and Keesom forces, constitute the long-range van der Waals forces. London's and Hamaker's work on the point-to-point dispersion interaction and Lifshitz's development of the continuum theory of dispersion are the foundations of our understanding of dispersion forces. Dispersion forces are present for all materials and are intrinsically related to the optical properties and the underlying interband electronic structures of materials. The force law scaling constant of the dispersion force, known as the Hamaker constant, can be determined from spectral or parametric optical properties of materials, combined with knowledge of the configuration of the materials. With recent access to new experimental and ab initio tools for determination of optical properties of materials, dispersion force research has new opportunities for detailed studies. Opportunities include development of improved index approximations and parametric representations of the optical properties for estimation of Hamaker constants. Expanded databases of London dispersion spectra of materials will permit accurate estimation of both nonretarded and retarded dispersion forces in complex configurations. Development of solutions for generalized multilayer configurations of materials are needed for the treatment of more-complex problems, such as graded interfaces. Dispersion forces can play a critical role in materials applications. Typically, they are a component with other forces in a force balance, and it is this balance that dictates the resulting behavior. The ubiquitous nature of the London dispersion forces makes them a factor in a wide spectrum of problems; they have been in evidence since the pioneering work of Young and Laplace on wetting, contact angles, and surface energies. Additional applications include the interparticle forces that can be measured by direct techniques, such as atomic force microscopy. London dispersion forces are important in both adhesion and in sintering, where the detailed shape at the crack tip and at the sintering neck can be controlled by the dispersion forces. Dispersion forces have an important role in the properties of numerous ceramics that contain intergranular films, and here the opportunity exists for the development of an integrated understanding of intergranular films that encompasses dispersion forces, segregation, multilayer adsorption, and structure. The intrinsic length scale at which there is a transition from the continuum perspective (dispersion forces) to the atomistic perspective (encompassing interatomic bonds) is critical in many materials problems, and the relationship of dispersion forces and intergranular films may represent an important opportunity to probe this topic. The London dispersion force is retarded at large separations, where the transit time of the electromagnetic interaction must be considered explicitly. Novel phenomena, such as equilibrium surficial films and bimodal wetting/dewetting, can result in materials systems when the characteristic wavelengths of the interatomic bonds and the physical interlayer thicknesses lead to a change in the sign of the dispersion force. Use of these novel phenomena in future materials applications provides interesting opportunities in materials design. [source] Regulation of In Situ Skeletal Muscle Arteriolar Tone: Interactions Between Two ParametersMICROCIRCULATION, Issue 6 2002JEFFERSON C. FRISBEEArticle first published online: 26 JAN 2010 ABSTRACT Objective: The growing understanding of the complexity of mechanisms regulating arteriolar tone demands that a systematic determination of how these processes interact to alter diameter be undertaken. This study examined how five mediators of skeletal muscle distal arteriolar tone [adenosine concentration, oxygen content, ,-adrenergic activation (norepinephrine), intravascular pressure and wall shear rate], taken two parameters at a time, interact to regulate vessel diameter. Methods: The reactivity of distal arterioles of in situ rat cremaster muscle after alterations in each of the above mediators was assessed. In addition, arteriolar responses to all two-parameter combinations were evaluated to determine the effect of altered environment on vascular reactivity to stimuli. Results: Arteriolar dilation to adenosine was unaltered by changes in other parameters. In contrast, wall shear rate-induced arteriolar dilation was impaired by 60,88% after increases in the other parameters. Myogenic reactivity was reduced by 28% with elevated O2 and by 65% with norepinephrine (because of vessel closure) and was impaired by 89% with elevated adenosine. O2 -induced arteriolar reactivity was impaired by 56% with increased adenosine and by 44% with increased norepinephrine concentration but was largely unaffected by elevated intravascular pressure. Adrenergic reactivity was attenuated with elevated intravascular pressure (by 69%) and O2 (by 54%) because of vessel closure but was unaltered with elevated adenosine. Conclusions: These data suggest that (1) individual mediators contributing to the regulation of arteriolar tone exist within a hierarchy of importance and (2) mechanisms regulating arteriolar tone can be impacted by unidentified alterations in other processes. Ongoing investigation into interactions between multiple processes regulating arteriolar tone will allow for a more integrated understanding of how microvessels regulate their diameter. [source] Making sense of accountability: Conceptual perspectives for northern and southern nonprofitsNONPROFIT MANAGEMENT & LEADERSHIP, Issue 2 2003Alnoor Ebrahim This article examines the concept of accountability from various disciplinary lenses in order to develop an integrated understanding of the term. Special attention is devoted to principal,agent perspectives from political science and economics. An integrated framework is developed, based on four central observations. (1) Accountability is relational in nature and is constructed through inter- and intraorganizational relationships. (2) Accountability is complicated by the dual role of nonprofits as both principals and agents in their relationships with other actors. (3) Characteristics of accountability necessarily vary with the type of nonprofit organization being examined. (4) Accountability operates through external as well as internal processes, such that an emphasis on external oversight and control misses other dimensions of accountability essential to nonprofit organizations. The analysis draws from the experiences of both Northern and Southern nonprofits, that is, organizations based in wealthy industrialized regions of the world (the global North) and those in economically poorer areas (the South). [source] Seeking red herrings in the wood: tending the shared spaces of environmental and feminist geographiesTHE CANADIAN GEOGRAPHER/LE GEOGRAPHE CANADIEN, Issue 1 2007MAUREEN G. REED In this article I argue the need for feminist and environmental geographers to work more diligently to find, mind and tend the intersections of their research agendas to enrich scholarship and deepen impacts on public policy. Such a project requires us to move beyond an obvious call to acknowledge one another's work and towards the boundaries of our respective fields in order to co-create ,boundary objects' that provide opportunities for mutual exchange, collaboration and learning. Rather than being ,red herrings' or diversions from our main research foci, boundary objects bring new insights to taken-for-granted concepts. I focus on one example to argue that social sustainability of rural places is better understood by an integrated understanding of what constitutes a ,worker' in a forestry community. A redefinition of the worker that draws on insights and interests from both environmental and feminist geographers reveals an underlying gender bias in environmental decision-making processes and illustrates how the concept of social sustainability has been artificially restricted in practice. Nevertheless, collaborations are never easy. I draw attention to potential challenges of such collaborations that include the need to establish mutually agreeable protocols, joint commitment to constructive, respectful debate and strategies to ensure that research provides meaningful contributions to theory and public policy. Dans cet article, je vais tenter de montrer que les géographes féministes et les géographes de l'environnement auront à travailler avec plus d'acharnement pour reconnaître, étudier et entretenir les points communs de leurs agendas de recherche en vue d'augmenter la valeur scientifique des études et d'accroître les retombées sur les politiques publiques. Un tel projet exige que nous puissions dépasser le stade de la reconnaissance de nos travaux et se tourner ainsi vers les frontières de nos disciplines afin de co-créer des ,objets frontaliers' qui offrent des possibilités d'échanges d'idées, de collaboration et d'apprentissage. Plutôt qu'être des ,fausses pistes' ou des déviations de nos principaux thèmes de recherche, ces objets frontaliers pourraient apporter un éclairage nouveau sur des concepts tenus pour acquis. À l'aide d'un exemple, j'avance que pour mieux comprendre la durabilité sociale en milieux ruraux, il faut acquérir une compréhension intégrée de l'ensemble des dimensions d'un ,ouvrier' membre d'une communauté forestière. L'ouvrier est redéfini en mettant à contribution les connaissances et intérêts des géographes environnementaux et féministes. Cette définition met au jour un parti pris fondé sur le sexe dans les processus décisionnels en matière d'environnement et démontre de quelle façon le concept de la durabilité sociale s'en trouve artificiellement restreint dans la pratique. Les collaborations ne sont cependant jamais faciles àétablir. J'attire l'attention sur les difficultés potentielles de ces collaborations concernant notamment la mise en place de protocoles acceptables pour les deux parties, la promesse de tenir des débats constructifs et respectueux, et les stratégies visant à garantir que la recherche participe de manière vitale autant au développement de la théorie que des politiques publiques. [source] Soil fertility, heterogeneity, and microbes: towards an integrated understanding of grassland structure and dynamicsAPPLIED VEGETATION SCIENCE, Issue 1 2009Heather L. Reynolds Abstract Objective: To highlight the need and the potential for an integrated understanding of three key soil-based drivers of plant community structure and dynamics , soil fertility, soil heterogeneity, and microbes. Location: European and North American grasslands. Methods: Review and discussion of conceptual models and empirical literature, including examples of observational and manipulative studies from both natural and restored grassland communities. Results and Conclusions: In general, the results of empirical studies on soil fertility, soil heterogeneity, and soil microbes in grassland communities do not support expectations of common conceptual models. Ecological theory assumes a unimodal relationship between soil fertility and plant community diversity, yet empirical relationships from grassland communities are variable, the mechanisms underlying these variable patterns are not yet well understood, and there is mixed success at manipulating soil fertility to facilitate restorations. While theory predicts that increased soil heterogeneity will lead to increased plant community diversity, results of experimental manipulations of soil heterogeneity often show the opposite. Of two major conceptual models proposed for how microbes structure plant communities, there is little support for the hypothesis of microbially mediated niche partitioning. Plant-microbe feedbacks do have significant empirical support to date and there is increasing application of positive feedback dynamics in restoration, yet field tests of feedback dynamics remain limited. We suggest that an understanding of interactions between these soil drivers may help to resolve discrepancies between conceptual models and empirical results, improving our understanding of grasslands and our ability to restore them. [source] Structure of the adenylation domain of NAD+ -dependent DNA ligase from Staphylococcus aureusACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 11 2009Seungil Han DNA ligase catalyzes phosphodiester-bond formation between immediately adjacent 5,-phosphate and 3,-hydroxyl groups in double-stranded DNA and plays a central role in many cellular and biochemical processes, including DNA replication, repair and recombination. Bacterial NAD+ -dependent DNA ligases have been extensively characterized as potential antibacterial targets because of their essentiality and their structural distinction from human ATP-dependent DNA ligases. The high-resolution structure of the adenylation domain of Staphylococcus aureus NAD+ -dependent DNA ligase establishes the conserved domain architecture with other bacterial adenylation domains. Two apo crystal structures revealed that the active site possesses the preformed NAD+ -binding pocket and the `C2 tunnel' lined with hydrophobic residues: Leu80, Phe224, Leu287, Phe295 and Trp302. The C2 tunnel is unique to bacterial DNA ligases and the Leu80 side chain at the mouth of the tunnel points inside the tunnel and forms a narrow funnel in the S. aureus DNA ligase structure. Taken together with other DNA ligase structures, the S. aureus DNA ligase structure provides a basis for a more integrated understanding of substrate recognition and catalysis and will be also be of help in the development of small-molecule inhibitors. [source] | ||||||||||||||||