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Selected AbstractsInfluence of therapy on the antioxidant status in patients with melanomaJOURNAL OF CLINICAL PHARMACY & THERAPEUTICS, Issue 2 2008V. Gadjeva DSc Summary Background and objective:, Some anticancer drugs can result in increased production of reactive oxygen species (ROS). Alkylating agents are the most frequently used drugs in chemotherapeutic regimens for the treatment of malignant melanoma. It is known that triazenes exhibit in vivo activity by alkylation of nucleic acids and proteins, but there is no data about ROS formation during oxidative metabolism. Single agents of most interest for treatment of malignant melanomas include 5-(3,3-dimethyltriazene-1-yl)-imidazole-4-carboxamide (DTIC) and nitrosoureas such as 1-(2-chloroethyl) -3-cyclohexyl-1-nitrosourea (CCNU), but complete response to these drugs is rare. The present study aimed to determine whether an oxidative stress occurs during the clinical course of melanoma and the influence of therapy on the antioxidant status of patients with melanoma. For this purpose, we investigated plasma concentrations of MDA as indices of the levels of lipid peroxidation products. In addition, we studied the activities of the antioxidant enzymes superoxide dismutases (SOD) and catalase (CAT) in patients with melanoma before any treatment, after surgical removal of melanoma, and after chemotherapy with DTIC or in combination with CCNU of the operated patients. Methods:, Twenty one patients with melanoma were studied. Patients were operated prior to chemotherapy. After recovery for 10,20 days postoperatively, they were studied again for MDA, SOD and CAT activity. The patients were divided into two groups according to the chemotherapy (3,7 treatment cycles): with DTIC , given orally daily for 5 days, every 3 weeks as a single 2200 mg/kg dose and with the combination , DTIC (the same dose) + CCNU , administered orally at a dosage of 120 mg/m2 once every 40 days in accordance with protocols, approved by the Bulgarian Ministry of Health. The total amount of lipid peroxidation products in plasma was assayed. Results and discussion:, Plasma levels of MDA and CAT activity were significantly higher, and erythrocyte SOD activity significantly lower, in patients with melanoma, than in control healthy volunteers (P < 0·0001). Ten to twenty days after surgery, oxidative stress decreased but levels of MDA increased as a result of therapy. Important sources of increased ROS production may be the monocytes, phagocytosis of tumour cells and the cancer tissues. Plasma MDA in patients treated with DTIC + CCNU were significantly higher (P < 0·001), but erythrocyte SOD statistically lower (P < 0·00001), compared with patients treated with DTIC only. However, a combination of DTIC + CCNU did not attenuate oxidative stress, or reduced antioxidant status. Patients treated with this combination are at bigger risk of oxidative injury. Therefore, this disturbance might be due to augmented generation of toxic ROS, possibly from the metabolism of CCNU. Conclusion:, Increased oxidative stress follows an imbalance in antioxidant defence in non-treated patients with melanoma. The impaired antioxidant system favours accumulation of ROS, which may promote the cancer process. After complete removal of melanoma tissues, oxidative stress decreased. The antioxidant status of melanoma patients operated on was influenced by the different chemotherapeutic regimens used and may play an important role in the response. Patients on DTIC + CCNU are at higher risk of oxidative injury. This drug combination probably exerts its toxic activity by ROS, which could be products of the metabolism of CCNU. [source] Biological Sustainability of Live Shearing of Vicuña in PeruCONSERVATION BIOLOGY, Issue 1 2007CATHERINE TERESA SAHLEY Andes; conservación basada en comunidades; Vicugna vicugna Abstract:,The vicuña's (Vicugna vicugna) fiber is highly valued as an export product that is made into luxury fabric and clothing. The price of fiber in 2004 was $566/kg, which makes the fiber a potentially important source of income for Andean agropastoral communities and serves as an incentive to allow vicuña grazing on high-elevation Andean landscapes. It is presumed that a shorn vicuña has little value for poachers, so shearing vicuñas could serve as a disincentive to poaching. Thus, the supply of vicuña fiber may be sustainable if it is procured through live shearing, which should serve as a powerful conservation tool. We evaluated the effects of capture and shearing on the demography of vicuña in one site located in the Salinas Aguada Blanca Reserve, Arequipa, Peru, where vicuñas were captured and shorn in spring and then returned to the wild. We conducted fixed-width line-transect censuses from 1997 to 2003 of this population. We compared the proportion of young born to females that were shorn versus females that were unshorn for the 3 years in which shearing occurred. We evaluated the effect of capture and shearing on proportion of young born to shorn and unshorn females at a second site, Picotani, Puno. The wild population in Arequipa that underwent capture and shearing showed a steady increase in total population and average density between 1997 and 2003. No significant difference was found between the proportion of young per female for shorn and unshorn females at either site. We conclude that in spring, capture and live shearing of vicuñas can be biologically sustainable. Further research is needed to determine whether shearing during winter months is biologically sustainable. Resumen:,La fibra de vicuña (Vicugna vicugna) tiene gran valor como un producto de exportación que es transformado en tela y ropa de lujo. El precio de la fibra en 2004 era de $566/kg, lo que hace que la fibra sea una fuente de ingreso potencialmente importante para comunidades agropastoriles Andinas y servir como un incentivo para permitir el pastoreo de vicuñas en paisajes Andinos elevados. Se presume que una vicuña trasquilada tiene poco valor para cazadores furtivos, por lo que el trasquilado de vicuñas pudiera servir como un desincentivo para la caza furtiva. Por lo tanto, el abastecimiento de fibra de vicuña puede ser sustentable si se obtiene del trasquilado de animales vivos, y el trasquilado de animales vivos debería ser una poderosa herramienta de conservación. Evaluamos los efectos de la captura y trasquilado sobre la demografía de vicuñas en un sitio localizado en la Reserva Salinas Aguada Blanca, Arequipa, Perú, donde las vicuñas fueron capturadas y trasquiladas en primavera y liberadas. Realizamos censos de esta población en transectos lineales de ancho fijo de 1997 a 2003. Comparamos la proporción de crías de hembras trasquiladas con las de hembras no trasquiladas durante los 3 años en que ocurrió el trasquilado. Evaluamos el efecto de la captura y trasquilado sobre la proporción de crías de hembras trasquiladas y no trasquiladas en un segundo sitio, Picotani, Puno. La población silvestre en Arequipa que fue capturada y trasquilada mostró un incremento constante en la población total y la densidad promedio entre 1997 y 2003. No se encontró diferencia significativa entre la proporción de crías por hembra para hembras trasquiladas y no trasquiladas en ningún sitio. Concluimos que en la primavera, la captura y trasquilado de vicuñas vivas puede ser biológicamente sostenible. Se requiere más investigación para determinar si el trasquilado durante el invierno es biológicamente sostenible. [source] Quantitative aspects of contact allergy to chromium and exposure to chrome-tanned leatherCONTACT DERMATITIS, Issue 3 2002Malene Barré Hansen The potential of trivalent and hexavalent chromium to induce and elicit allergic contact dermatitis and the degree of chromium exposure from leather products are reviewed. Chromium dermatitis is often due to exposure in the occupational environment, with cement being one of the most common chromium sources. However, consumer products such as chromium(III)-tanned leather products are also an important source of chromium exposure. Apart from Cr(III), which is used for tanning, leather often also contains trace amounts of Cr(VI), which is formed by oxidation of Cr(III) during the tanning process. In a recent study of the Cr(VI) content of leather products bought on the Danish market, 35% of such articles had a Cr(VI) content above the detection limit of 3 p.p.m., ranging from 3.6 p.p.m. to 14.7 p.p.m. Leachable Cr(III) was detected at levels of 430,980 p.p.m. An examination of available dose,response studies showed that exposure to occluded patch test concentrations of 7,45 p.p.m. Cr(VI) elicits a reaction in 10% of the chromium-sensitive patients. When reviewing repeated open exposure studies, it is seen that either exposure to 5 p.p.m. Cr(VI) in the presence of 1% sodium lauryl sulfate (SLS) or exposure to 10 p.p.m. Cr(VI) alone both elicit eczema in chromium-sensitive patients. The eliciting capacity of Cr(III) has not been systematically investigated but, compared to Cr(VI), much higher concentrations are needed to elicit eczema. [source] Benefit,Cost Appraisals of Export Processing Zones: A Survey of the LiteratureDEVELOPMENT POLICY REVIEW, Issue 1 2003Kankesu Jayanthakumaran This article surveys research on the performance of Export Processing Zones (EPZs) using a benefit,cost analytical framework. Results suggest that zones in South Korea, Malaysia, Sri Lanka, China and Indonesia are economically efficient and generate returns well above estimated opportunity costs. On the other hand, the heavy infrastructure costs involved in setting up the zone in the Philippines resulted in a negative net present value. The zones have been an important source of employment in all cases and have promoted local entrepreneurs in some. However, as industrial development proceeds, the gap between the market and opportunity costs of labour narrows and the interest in EPZs tends to disappear. It may hold only if the zones generate private profit to domestic shareholders. [source] The concentrations of short-chain fatty acids and other microflora-associated characteristics in faeces from children with newly diagnosed Type 1 diabetes and control children and their family membersDIABETIC MEDICINE, Issue 1 2004U. Samuelsson Abstract Aims The gut flora is quantitatively the most important source of microbial stimulation and may provide a primary signal in the maturation of the immune system. We compared the microflora-associated characteristics (MACs) in 22 children with newly diagnosed diabetes, 27 healthy controls, and their family members to see if there were differences between the children and if there was a familial pattern. Methods The MACs were assessed by determining the concentrations of eight short-chain fatty acids (SCFA), mucin, urobilin, b-aspartylglycine, coprastanol and faecal tryptic activity (FTA). Results There were no statistically significant differences between the concentrations of SCFA in the diabetes and control children. Members of families with a diabetic child had a higher concentration of acetic acid (P < 0.02) and lower concentrations of several other SCFAs than control families (P < 0.05,0.02). The other MACs showed no differences between the children or between the two family groups. Conclusion In this pilot study we saw no differences in the MACs between children with diabetes and their controls. There were, however, some differences between the family members of diabetic children and controls that may indicate a familial pattern regarding the production of SCFAs by the gut flora. The role of the gut flora in relation to the risk of developing Type 1 diabetes needs to be analysed in larger and/or prospective studies. [source] Processes controlling rapid temperature variations on rock surfaces,EARTH SURFACE PROCESSES AND LANDFORMS, Issue 5 2010Jamie L. Molaro Abstract In arid environments, thermal oscillations are an important source of rock weathering. Measurements of temperature have been made on the surface of rocks in a desert environment at a sampling interval of 0·375,s, with simultaneous measurements of wind speed, air temperature, and incoming shortwave radiation. Over timescales of hours, the temperature of the rock surface was determined primarily by shortwave radiation and air temperature, while rapid temperature variations, high dT/dt, at intervals of seconds or less, were determined by wind speed. The maximum values of temperature change and time spent above 2°C,min,1 increased at high measurement rates and were much higher than previously reported. The maximum recorded value of dT/dt was 137°C,min,1 and the average percentage time spent above 2°C,min,1 was ,70 ± 13%. Maximum values of dT/dt did not correlate with the maximum values of time spent above 2°C,min,1. Simultaneous measurements of two thermocouples 5·5,cm apart on a single rock surface had similar temperature and dT/dt values, but were not correlated at sampling intervals of less than 10,s. It is suggested that this is resulting from rapid fluctuations due to small spatial and timescale wind effects that are averaged out when data is taken at longer sampling intervals, ,10,s or greater. Published in 2010 by John Wiley & Sons, Ltd. [source] Sources and sediment yield from a rural catchment in humid temperate environment, northwest SpainEARTH SURFACE PROCESSES AND LANDFORMS, Issue 3 2010M. L. Rodríguez-Blanco Abstract A study was carried out on a rural catchment located in northwest Spain to examine the sediment yield from the catchment by measuring suspended sediments during rainfall events. Within the catchment regular surveys were conducted to obtain data on the suspended sediment sources. Important variations in sediment load were detected at event scale (0·3,21·0,Mg); some of these can be explained in terms of event size, antecedent conditions, rainfall distribution and soil surface erosion. To study the variables controlling suspended sediment yield during the events in the catchment, several event and pre-event variables were calculated for all events. The sediment load is strongly influenced by discharge variables. During the events discharge,suspended sediments were also analysed. When the soil surface was unprotected, the formation of rills and ephemeral gullies on agricultural land at the catchment head was an important source of suspended sediments in the catchment. Copyright © 2010 John Wiley & Sons, Ltd. [source] The implications of data selection for regional erosion and sediment yield modellingEARTH SURFACE PROCESSES AND LANDFORMS, Issue 15 2009Joris de Vente Abstract Regional environmental models often require detailed data on topography, land cover, soil, and climate. Remote sensing derived data form an increasingly important source of information for these models. Yet, it is often not easy to decide what the most feasible source of information is and how different input data affect model outcomes. This paper compares the quality and performance of remote sensing derived data for regional soil erosion and sediment yield modelling with the WATEM-SEDEM model in south-east Spain. An ASTER-derived digital elevation model (DEM) was compared with the DEM obtained from the Shuttle Radar Topography Mission (SRTM), and land cover information from the CORINE database (CLC2000) was compared with classified ASTER satellite images. The SRTM DEM provided more accurate estimates of slope gradient and upslope drainage area than the ASTER DEM. The classified ASTER images provided a high accuracy (90%) land cover map, and due to its higher resolution, it showed a more fragmented landscape than the CORINE land cover data. Notwithstanding the differences in quality and level of detail, CORINE and ASTER land cover data in combination with the SRTM DEM or ASTER DEM allowed accurate predictions of sediment yield at the catchment scale. Although the absolute values of erosion and sediment deposition were different, the qualitative spatial pattern of the major sources and sinks of sediments was comparable, irrespective of the DEM and land cover data used. However, due to its lower accuracy, the quantitative spatial pattern of predictions with the ASTER DEM will be worse than with the SRTM DEM. Therefore, the SRTM DEM in combination with ASTER-derived land cover data presumably provide most accurate spatially distributed estimates of soil erosion and sediment yield. Nevertheless, model calibration is required for each data set and resolution and validation of the spatial pattern of predictions is urgently needed. Copyright © 2009 John Wiley & Sons, Ltd. [source] Vegetation and topographic controls on sediment deposition and storage on gully beds in a degraded mountain areaEARTH SURFACE PROCESSES AND LANDFORMS, Issue 6 2009Armando Molina Abstract Active gully systems developed on highly weathered or loose parent material are an important source of runoff and sediment production in degraded areas. However, a decrease of land pressure may lead to a return of a partial vegetation cover, whereby gully beds are preferred recolonization spots. Although the current knowledge on the role of vegetation on reducing sediment production on slopes is well developed, few studies exist on the significance of restoring sediment transport pathways on the total sediment budget of degraded mountainous catchments. This study in the Ecuadorian Andes evaluates the potential of vegetation to stabilize active gully systems by trapping and retaining eroded sediment in the gully bed, and analyses the significance of vegetation restoration in the gully bed in reducing sediment export from degraded catchments. Field measurements on 138 gully segments located in 13 ephemeral steep gullies with different ground vegetation cover indicate that gully bed vegetation is the most important factor in promoting short-term (1,15 years) sediment deposition and gully stabilization. In well-vegetated gully systems ( , 30% of ground vegetation cover), 0.035 m3 m,1 of sediment is deposited yearly in the gully bed. Almost 50 per cent of the observed variance in sediment deposition volumes can be explained by the mean ground vegetation cover of the gully bed. The presence of vegetation in gully beds gives rise to the formation of vegetated buffer zones, which enhance short-term sediment trapping even in active gully systems in mountainous environments. Vegetation buffer zones are shown to modify the connectivity of sediment fluxes, as they reduce the transport efficiency of gully systems. First calculations on data on sediment deposition patterns in our study area show that gully bed deposition in response to gully bed revegetation can represent more than 25 per cent of the volume of sediment generated within the catchment. Our findings indicate that relatively small changes in landscape connectivity have the potential to create strong (positive) feedback loops between erosion and vegetation dynamics. Copyright © 2009 John Wiley & Sons, Ltd. [source] Evaluation of the PESERA model in two contrasting environmentsEARTH SURFACE PROCESSES AND LANDFORMS, Issue 5 2009F. Licciardello Abstract The performance of the Pan-European Soil Erosion Risk Assessment (PESERA) model was evaluated by comparison with existing soil erosion data collected in plots under different land uses and climate conditions in Europe. In order to identify the most important sources of error, the PESERA model was evaluated by comparing model output with measured values as well as by assessing the effect of the various model components on prediction accuracy through a multistep approach. First, the performance of the hydrological and erosion components of PESERA was evaluated separately by comparing both runoff and soil loss predictions with measured values. In order to assess the performance of the vegetation growth component of PESERA, the predictions of the model based on observed values of vegetation ground cover were also compared with predictions based on the simulated vegetation cover values. Finally, in order to evaluate the sediment transport model, predicted monthly erosion rates were also calculated using observed values of runoff and vegetation cover instead of simulated values. Moreover, in order to investigate the capability of PESERA to reproduce seasonal trends, the observed and simulated monthly runoff and erosion values were aggregated at different temporal scale and we investigated at what extend the model prediction error could be reduced by output aggregation. PESERA showed promise to predict annual average spatial variability quite well. In its present form, short-term temporal variations are not well captured probably due to various reasons. The multistep approach showed that this is not only due to unrealistic simulation of cover and runoff, being erosion prediction also an important source of error. Although variability between the investigated land uses and climate conditions is well captured, absolute rates are strongly underestimated. A calibration procedure, focused on a soil erodibility factor, is proposed to reduce the significant underestimation of soil erosion rates. Copyright © 2009 John Wiley & Sons, Ltd. [source] A sediment budget for a cultivated floodplain in tropical North Queensland, AustraliaEARTH SURFACE PROCESSES AND LANDFORMS, Issue 10 2007Fleur Visser Abstract Sugarcane is grown on the floodplains of northern Queensland adjacent to the Great Barrier Reef lagoon. Sediment and nutrient loss from these sugarcane areas is considered a potential threat to coastal and marine ecosystems. To enable sugarcane cultivation, farmers have structured the landscape into different elements, comprising fields, water furrows, ,headlands' and drains. In order to apply appropriate management of the landscape and reduce export of sediment, it is important to identify which of these elements act as sediment sources or sinks. In this study erosion and deposition rates were measured for the different landscape elements in a subcatchment of the Herbert River and used to create a sediment budget. Despite large uncertainties, the budget shows that the floodplain area is a net source of sediment. Estimated sediment export varies between 2 and 5 t ha,1 y,1. The relative importance of the landscape elements as sediment sources could also be determined. Plant cane is identified as the most important sediment source. Water furrows generate most sediment, but are a less important source of exported sediment due to their low connectivity. Headlands and minor drains act as sediment traps. Copyright © 2007 John Wiley & Sons, Ltd. [source] Taxation, warfare, and the early fourteenth century ,crisis' in the north: Cumberland lay subsidies, 1332,13481ECONOMIC HISTORY REVIEW, Issue 4 2005CHRIS BRIGGS Recent research into the impact of Anglo-Scottish conflict on northern England's economy has become increasingly sophisticated, using local estate accounts to enhance understanding of the role of war in the 'crisis' of the early fourteenth century. Yet taxation data also remains an important source on these issues, not least because of its wide geographical coverage. Using a rich series of lay subsidy documents for Cumberland, this article concludes that the direct impact of Scottish raids was only one of several determinants of economic fortunes. More significantly, reconstructing the process of taxation shows that non-violent resistance to state levies was as responsible as war damage for a decline in revenue from the county. [source] The Entrepreneurial Propensity of WomenENTREPRENEURSHIP THEORY AND PRACTICE, Issue 3 2007Nan Langowitz Entrepreneurship is becoming an increasingly important source of employment for women across many countries. The level of female involvement in entrepreneurial activity, however, is still significantly lower than that of men. We take a behavioral economics approach and, using a large sample of individuals in 17 countries, we investigate what variables influence the entrepreneurial propensity of women and whether those variables have a significant correlation with differences across genders. In addition to demographic and economic variables, we include a number of perceptual variables. Our results show that subjective perceptual variables have a crucial influence on the entrepreneurial propensity of women and account for much of the difference in entrepreneurial activity between the sexes. Specifically, we find that women tend to perceive themselves and the entrepreneurial environment in a less favorable light than men across all countries in our sample and regardless of entrepreneurial motivation. Our results suggest that perceptual variables may be significant universal factors influencing entrepreneurial behavior. [source] Role of menaquinone biosynthesis genes in selenate reduction by Enterobacter cloacae SLD1a-1 and Escherichia coli K12ENVIRONMENTAL MICROBIOLOGY, Issue 1 2009Jincai Ma Summary In this study, we investigated the role of menaquinone biosynthesis genes in selenate reduction by Enterobacter cloacae SLD1a-1 and Escherichia coli K12. A mini-Tn5 transposon mutant of E. cloacae SLD1a-1, designated as 4E6, was isolated that had lost the ability to reduce Se(VI) to Se(0). Genetic analysis of mutant strain 4E6 showed that the transposon was inserted within a menD gene among a menFDHBCE gene cluster that encodes for proteins required for menaquinone biosynthesis. A group of E. coli K12 strains with single mutations in the menF, menD, menC and menE genes were tested for loss of selenate reduction activity. The results showed that E. coli K12 carrying a deletion of either the menD, menC or menE gene was unable to reduce selenate. Complementation using wild-type sequences of the E. cloacae SLD1a-1 menFDHBCE sequence successfully restored the selenate reduction activity in mutant strain 4E6, and E. coli K12 menD and menE mutants. Selenate reduction activity in 4E6 was also restored by chemical complementation using the menaquinone precursor compound 1,4-dihydroxy-2-nathphoic acid. The results of this work suggest that menaquinones are an important source of electrons for the selenate reductase, and are required for selenate reduction activity in E. cloacae SLD1a-1 and E. coli K12. [source] Diversity and expression of nitrogen fixation genes in bacterial symbionts of marine spongesENVIRONMENTAL MICROBIOLOGY, Issue 11 2008Naglaa M. Mohamed Summary Marine sponges contain complex assemblages of bacterial symbionts, the roles of which remain largely unknown. We identified diverse bacterial nifH genes within sponges and found that nifH genes are expressed in sponges. This is the first demonstration of the expression of any protein-coding bacterial gene within a sponge. Two sponges Ircinia strobilina and Mycale laxissima were collected from Key Largo, Florida and had ,15N values of c. 0,1, and 3,4, respectively. The potential for nitrogen fixation by symbionts was assessed by amplification of nifH genes. Diverse nifH genes affiliated with Proteobacteria and Cyanobacteria were detected, and expression of nifH genes affiliated with those from cyanobacteria was detected. The nifH genes from surrounding seawater were similar to those of Trichodesmium and clearly different from the cyanobacterial nifH genes detected in the two sponges. This study advances understanding of the role of bacterial symbionts in sponges and suggests that provision of fixed nitrogen is a means whereby symbionts benefit sponges in nutrient-limited reef environments. Nitrogen fixation by sponge symbionts is possibly an important source of new nitrogen to the reef environment that heretofore has been neglected and warrants further investigation. [source] Nitrogenase gene diversity and microbial community structure: a cross-system comparisonENVIRONMENTAL MICROBIOLOGY, Issue 7 2003Jonathan P. Zehr Summary Biological nitrogen fixation is an important source of fixed nitrogen for the biosphere. Microorganisms catalyse biological nitrogen fixation with the enzyme nitrogenase, which has been highly conserved through evolution. Cloning and sequencing of one of the nitrogenase structural genes, nifH, has provided a large, rapidly expanding database of sequences from diverse terrestrial and aquatic environments. Comparison of nifH phylogenies to ribosomal RNA phylogenies from cultivated microorganisms shows little conclusive evidence of lateral gene transfer. Sequence diversity far outstrips representation by cultivated representatives. The phylogeny of nitrogenase includes branches that represent phylotypic groupings based on ribosomal RNA phylogeny, but also includes paralogous clades including the alternative, non-molybdenum, non-vanadium containing nitrogenases. Only a few alternative or archaeal nitrogenase sequences have as yet been obtained from the environment. Extensive analysis of the distribution of nifH phylotypes among habitats indicates that there are characteristic patterns of nitrogen fixing microorganisms in termite guts, sediment and soil environments, estuaries and salt marshes, and oligotrophic oceans. The distribution of nitrogen-fixing microorganisms, although not entirely dictated by the nitrogen availability in the environment, is non-random and can be predicted on the basis of habitat characteristics. The ability to assay for gene expression and investigate genome arrangements provides the promise of new tools for interrogating natural populations of diazotrophs. The broad analysis of nitrogenase genes provides a basis for developing molecular assays and bioinformatics approaches for the study of nitrogen fixation in the environment. [source] Mechanical stretch induces TGF-, synthesis in hepatic stellate cellsEUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 2 2004R. Sakata Abstract Background, It is known that mechanical stress induces extracellular matrix via transforming growth factor-, (TGF-,) synthesis in vascular smooth muscle cells. Activated hepatic stellate cells (HSCs) are an important source of TGF-, in the liver. However, it remains unclear whether mechanical stress induces TGF-, in HSCs. The Rho small GTP-binding protein (Rho) has recently emerged as an important regulator of actin and cytoskeleton. We examined whether TGF-, is expressed in stretched HSCs and whether Rho is involved in stretch-induced TGF-, synthesis. Materials and methods, A cultured human HSC cell line, LI90, was used for this study. Hepatic stellate cells were cyclically stretched using the Flexercell® strain unit. Concentration of TGF-, in the conditioned medium was estimated by a bioassay using mink lung epithelial cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Transforming growth factor-, mRNA expression of HSCs was estimated by a reverse-transcription polymerase chain reaction. Replication-defective adenoviral vectors expressing a dominant negative type of Rho was utilized to suppress its effect on HSCs. Results, Transforming growth factor-, concentration of the conditioned media of stretched HSCs showed time-dependent increases as compared to nonstretched HSCs from 2 h to 24 h. Transforming growth factor-, mRNA expression in stretched HSCs was increased compared with that in nonstretched HSCs. Transfection of dominant negative Rho inhibited the stretch-induced TGF-, synthesis. Conclusions, Mechanical stretch enhanced TGF-, expression on mRNA and protein level in HSCs. Rho was closely related to stretch-induced TGF-, synthesis in HSCs. [source] Presynaptic source of quantal size variability at GABAergic synapses in rat hippocampal neurons in cultureEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 7 2004Andrea Barberis Abstract The variability of quantal size depends on both presynaptic (profile of the neurotransmitter concentration in the cleft) and postsynaptic (number and gating properties of postsynaptic receptors) factors. Here we have examined the possibility that at nonsaturated synapses in cultured hippocampal neurons, changes in both the transmitter concentration peak and its clearance from the synaptic cleft may influence the variability of spontaneous miniature synaptic GABAergic currents (mIPSCs). We found that, in contrast to the slow-off GABAA receptor antagonist bicuculline, fast-off competitive antagonists such as SR-95103 and TPMPA differentially blocked small and large mIPSCs. In the presence of flurazepam, a drug believed to increase the affinity of GABA for GABAAR, small mIPSCs were enhanced more efficiently than large events. Moreover, the addition of dextran, which increases the viscosity of the extracellular fluid, preferentially increased small mIPSCs with respect to large ones. These observations suggest that changes in the concentration peak and the speed of GABA clearance in the cleft may be an important source of synaptic variability. The study of the correlation between peak amplitude and kinetics of mIPSCs allowed determination of the relative contribution of transmitter peak concentration vs. time of GABA clearance. Small synaptic responses were associated with fast onset and decay kinetics while large amplitude currents were asociated with slow kinetics, indicating a crucial role for GABA synaptic clearance in variability of mIPSCs. By using model simulations we were able to estimate the range of variability of both the concentration and the speed of clearance of the GABA transient in the synaptic cleft. [source] Political participation and procedural utility: An empirical studyEUROPEAN JOURNAL OF POLITICAL RESEARCH, Issue 3 2006ALOIS STUTZER In this article, it is argued that people have preferences about both aspects and that they derive utility from the processes involved in decision making over and above the utility gained from outcomes. The authors study political participation possibilities as an important source of procedural utility. To distinguish between outcome and process utility, they take advantage of the fact that nationals can participate in political decision making, while foreigners are excluded and thus cannot enjoy the respective procedural utility. Utility is assumed to be measurable by individually reported subjective well-being. As an additional indicator for procedural utility, reported belief in political influence is analyzed. [source] HERITABILITY OF AND EARLY ENVIRONMENT EFFECTS ON VARIATION IN MATING PREFERENCESEVOLUTION, Issue 4 2010Holger Schielzeth Many species show substantial between-individual variation in mating preferences, but studying the causes of such variation remains a challenge. For example, the relative importance of heritable variation versus shared early environment effects (like sexual imprinting) on mating preferences has never been quantified in a population of animals. Here, we estimate the heritability of and early rearing effects on mate choice decisions in zebra finches based on the similarity of choices between pairs of genetic sisters raised apart and pairs of unrelated foster sisters. We found a low and nonsignificant heritability of preferences and no significant shared early rearing effects. A literature review shows that a low heritability of preferences is rather typical, whereas empirical tests for the relevance of sexual imprinting within populations are currently limited to very few studies. Although effects on preference functions (i.e., which male to prefer) were weak, we found strong individual consistency in choice behavior and part of this variation was heritable. It seems likely that variation in choice behavior (choosiness, responsiveness, sampling behavior) would produce patterns of nonrandom mating and this might be the more important source of between-individual differences in mating patterns. [source] RELATIVE CONTRIBUTION OF ADDITIVE, DOMINANCE, AND IMPRINTING EFFECTS TO PHENOTYPIC VARIATION IN BODY SIZE AND GROWTH BETWEEN DIVERGENT SELECTION LINES OF MICEEVOLUTION, Issue 5 2009Reinmar Hager Epigenetic effects attributed to genomic imprinting are increasingly recognized as an important source of variation in quantitative traits. However, little is known about their relative contribution to phenotypic variation compared to those of additive and dominance effects, and almost nothing about their role in phenotypic evolution. Here we address these questions by investigating the relative contribution of additive, dominance, and imprinting effects of quantitative trait loci (QTL) to variation in "early" and "late" body weight in an intercross of mice selected for divergent adult body weight. We identified 18 loci on 13 chromosomes; additive effects accounted for most of the phenotypic variation throughout development, and imprinting effects were always small. Genetic effects on early weight showed more dominance, less additive, and, surprisingly, less imprinting variation than that of late weight. The predominance of additivity of QTL effects on body weight follows the expectation that additive effects account for the evolutionary divergence between selection lines. We hypothesize that the appearance of more imprinting effects on late body weight may be a consequence of divergent selection on adult body weight, which may have indirectly selected for alleles showing partial imprinting effects due to their associated additive effects, highlighting a potential role of genomic imprinting in the response to selection. [source] YOUNG ADULTS' PERSPECTIVES ON DIVORCE Living ArrangementsFAMILY COURT REVIEW, Issue 4 2000William V. Fabricius There is increasing consensus that the perspectives of children need to be taken into account in decisions made by divorcing parents and the courts and that young adults who have lived through their parents' divorces can be an important source of information about children's perspectives. In this study, the authors assessed the perspectives of 820 college adults from divorced families on the issue of children's living arrangements after divorce. Respondents wanted to have spent more time with their fathers as they were growing up, and the living arrangement they believed was best was living equal time with each parent. The living arrangements they had as children gave them generally little time with their fathers. Respondents reported that their fathers wanted more time with them but that their mothers generally did not want them to spend more time with their fathers. [source] Molecular epidemiology of Yersinia enterocolitica infectionsFEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 3 2006Maria Fredriksson-Ahomaa Abstract Yersinia enterocolitica is an important food-borne pathogen that can cause yersiniosis in humans and animals. The epidemiology of Y. enterocolitica infections is complex and remains poorly understood. Most cases of yersiniosis occur sporadically without an apparent source. The main sources of human infection are assumed to be pork and pork products, as pigs are a major reservoir of pathogenic Y. enterocolitica. However, no clear evidence shows that such a transmission route exists. Using PCR, the detection rate of pathogenic Y. enterocolitica in raw pork products is high, which reinforces the assumption that these products are a transmission link between pigs and humans. Several different DNA-based methods have been used to characterize Y. enterocolitica strains. However, the high genetic similarity between strains and the predominating genotypes within the bio- and serotype have limited the benefit of these methods in epidemiological studies. Similar DNA patterns have been obtained among human and pig strains of pathogenic Y. enterocolitica, corroborating the view that pigs are an important source of human yersiniosis. Indistinguishable genotypes have also been found between human strains and dog, cat, sheep and wild rodent strains, indicating that these animals are other possible infection sources for humans. [source] The role of cysteine and cysteine,S conjugates as odour precursors in the flavour and fragrance industry ,FLAVOUR AND FRAGRANCE JOURNAL, Issue 6 2008Christian Starkenmann Abstract Volatile sulphur compounds are important odorants in the flavour and fragrance industries. Recent developments in the field have reinvigorated sulphur compound research, particularly with regard to the precursor compounds of volatile thiols. The present review concentrates on the role of cysteine and cysteine,S conjugates as precursor compounds for a variety of aromas and examines the chemical and enzymatic pathways of degradation and biotransformation. Cysteine is an important source of sulphur in flavour chemistry and may be degraded into hydrogen sulphide, which can further participate in other reactions. Direct reactions of cysteine and the pathways particular to various flavours and fragrances are discussed, with an emphasis on the precursor compounds cysteine,S conjugates. The importance of cysteine precursors in plants, including onion, wine, passion fruit, bitter orange, asparagus and bell pepper and the formation of natural scents in cats and humans are discussed. Copyright © 2008 John Wiley & Sons, Ltd. [source] Particulate fatty acids in two small Siberian reservoirs dominated by different groups of phytoplanktonFRESHWATER BIOLOGY, Issue 3 2003Nadezhda N. Sushchik SUMMARY 1. We studied the composition of fatty acids (FAs) in the seston from two small freshwater reservoirs (Bugach and Lesnoi) with distinct periodicity of domination by cyanobacteria and eukaryotic algae during the growth season. 2. The diatoms in the both reservoirs were characterised by a high content of 14:0 and C16 unsaturated acids, whereas that of the essential FA 20:5,3 [eicosapentanoic acid (EPA)] was low. The correlation between this polyunsaturated FA (PUFA) and diatom biomass was not significant in either reservoir. The percentage of 20:5,3 in seston significantly correlated with the biomass of euglenophyta in Bugach and dinophyta in Lesnoi. Hence the diatoms, usually referred as a valuable food for zooplankton, were not an important source of the essential PUFA in these systems. 3. The dominant cyanobacteria in Bugach, and the green algae in Lesnoi, both contained the same marker acids: 18:3,3 and 18:2,6. Hence, a discrimination between these two phytoplanktonic groups on the basis of FA biomarkers may be difficult in some cases. 4. We found no significant correlation between the content of 20:5,3 in seston and the biomass of the dominant daphniids in either reservoir. This is contrary to expectations, based on the literature, that EPA is generally important. Rather, the biomass of the two dominant Daphnia species in Bugach correlated strongly with the content of 18:3,3 in the seston. The cyanobacteria were a probable source of this ,3 FA for Daphnia. We conclude that EPA is not always important for Daphnia populations although, in such cases, some other PUFA (e.g. 18:3,3) might be related to their growth. [source] Parasites can cause selection against migrants following dispersal between environmentsFUNCTIONAL ECOLOGY, Issue 4 2010Andrew D. C. MacColl Summary 1.,The potential for selection against migrants to promote population divergence and speciation is well established in theory, yet there has been relatively little empirical work that has explicitly considered selection against migrants as a form of reproductive barrier, and its importance in the accumulation of reproductive isolation between populations has been overlooked until recently. 2.,Parasites often differ between environments and can be an important source of selection on hosts, yet their contribution to population divergence in general, and selection against migrants in particular, is poorly understood. 3.,Selection against migrants might be reduced if organisms escape parasitism when they disperse (natural enemy release). Alternatively, parasites could increase selection against migrants if, when they disperse, organisms encounter parasites to which they are poorly adapted. 4.,Here we test experimentally the contribution that parasites could make to selection against migrants in the adaptive radiation of three-spined sticklebacks. These fish have repeatedly colonized freshwater environments from marine ones, and this has repeatedly lead to rapid speciation. 5.,We use transplant experiments of lab-raised fish to simulate dispersal, and antihelminthic treatment to show that ancestral-type marine sticklebacks contract higher burdens of novel parasites when introduced to freshwater, than in saltwater, and suffer a growth cost as a direct result. 6.,Susceptibility to parasites and their detrimental effect is less in derived, freshwater fish from evolutionarily young populations, possibly as a result of selection for resistance. 7.,Our results support a role for parasites in selection against migrants and population diversification. They do not support the hypothesis of ,natural enemy release'. [source] Effects of low calcium concentrations on two common freshwater crustaceans, Gammarus lacustris and Astacus astacusFUNCTIONAL ECOLOGY, Issue 3 2002N. A. Rukke Summary 1.,Field studies indicate that low Ca concentrations limit the distribution and success of Ca-demanding freshwater crustaceans in soft-water localities. A long-lasting depletion of Ca caused by reversed acidification could thus be detrimental to such species. 2.,Experimental studies of Gammarus lacustris and Astacus astacus revealed decreased survival and retarded growth at ambient Ca concentrations <5 mg l,1, compared with animals reared at 10 mg l,1. 3.,A significant proportion of the total body Ca content was lost during the moult, but moulting frequencies were not affected by ambient Ca concentration. Complete postmoult calcification of the carapace could not be achieved at ambient Ca concentrations <5 mg l,1, and individuals reared at such conditions had a lower specific Ca content than individuals reared at sufficient Ca levels. 4.,A juvenile bottleneck regarding the susceptibility to Ca deficiency was shown in G. lacustris, as neonates had a relatively higher mass-specific Ca content and a higher Ca threshold for survival than adults. 5.,Because of a short postmoult calcification period of less than 2 days, G. lacustris had limited ability to compensate for low water Ca levels by obtaining Ca from food. However, food might be an important source of Ca for A. astacus living at low ambient Ca, because eating could be resumed during the relatively long calcification period (>15 days). [source] Enzymatic deconstruction of xylan for biofuel productionGCB BIOENERGY, Issue 1 2009DYLAN DODD Abstract The combustion of fossil-derived fuels has a significant impact on atmospheric carbon dioxide (CO2) levels and correspondingly is an important contributor to anthropogenic global climate change. Plants have evolved photosynthetic mechanisms in which solar energy is used to fix CO2 into carbohydrates. Thus, combustion of biofuels, derived from plant biomass, can be considered a potentially carbon neutral process. One of the major limitations for efficient conversion of plant biomass to biofuels is the recalcitrant nature of the plant cell wall, which is composed mostly of lignocellulosic materials (lignin, cellulose, and hemicellulose). The heteropolymer xylan represents the most abundant hemicellulosic polysaccharide and is composed primarily of xylose, arabinose, and glucuronic acid. Microbes have evolved a plethora of enzymatic strategies for hydrolyzing xylan into its constituent sugars for subsequent fermentation to biofuels. Therefore, microorganisms are considered an important source of biocatalysts in the emerging biofuel industry. To produce an optimized enzymatic cocktail for xylan deconstruction, it will be valuable to gain insight at the molecular level of the chemical linkages and the mechanisms by which these enzymes recognize their substrates and catalyze their reactions. Recent advances in genomics, proteomics, and structural biology have revolutionized our understanding of the microbial xylanolytic enzymes. This review focuses on current understanding of the molecular basis for substrate specificity and catalysis by enzymes involved in xylan deconstruction. [source] Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression of a protein containing 17 selenocysteine residuesGENES TO CELLS, Issue 12 2000Gregory V. Kryukov Fish are an important source of selenium in human nutrition and the zebrafish is a potentially useful model organism for the study of selenium metabolism and its role in biology and medicine. Selenium is present in vertebrate proteins in the form of selenocysteine (Sec), the 21st natural amino acid in protein which is encoded by UGA. We report here the detection of 18 zebrafish genes for Sec-containing proteins. We found two zebrafish orthologs of human SelT, glutathione peroxidase 1 and glutathione peroxidase 4, and single orthologs of several other selenoproteins. In addition, new zebrafish selenoproteins were identified that were distant homologues of SelP, SelT and SelW, but their direct orthologs in other species are not known. This multiplicity of selenoprotein genes appeared to result from gene and genome duplications, followed by the retention of new selenoprotein genes. We found a zebrafish selenoprotein P gene (designated zSelPa) that contained two Sec insertion sequence (SECIS) elements and encoded a protein containing 17 Sec residues, the largest number of Sec residues found in any known protein. In contrast, a second SelP gene (designated zSelPb) was also identified that contained one SECIS element and encoded a protein with a single Sec. We found that zSelPa could be expressed and secreted by mammalian cells. The occurrence of zSelPa and zSelPb suggested that the function of the N-terminal domain of mammalian SelP proteins may be separated from that of the C-terminal Sec-rich sequence: the N-terminal domain containing the UxxC motif is likely involved in oxidoreduction, whereas the C-terminal portion of the protein may function in selenium transport or storage. Our data also suggest that the utilization of Sec is more common in zebrafish than in previously characterized species, including mammals. [source] DNA repair pathways involved in anaphase bridge formationGENES, CHROMOSOMES AND CANCER, Issue 6 2007Ceyda Acilan Cancer cells frequently exhibit gross chromosomal alterations such as translocations, deletions, or gene amplifications an important source of chromosomal instability in malignant cells. One of the better-documented examples is the formation of anaphase bridges,chromosomes pulled in opposite directions by the spindle apparatus. Anaphase bridges are associated with DNA double strand breaks (DSBs). While the majority of DSBs are repaired correctly, to restore the original chromosome structure, incorrect fusion events also occur leading to bridging. To identify the cellular repair pathways used to form these aberrant structures, we tested a requirement for either of the two major DSB repair pathways in mammalian cells: homologous recombination (HR) and nonhomologous end joining (NHEJ). Our observations show that neither pathway is essential, but NHEJ helps prevent bridges. When NHEJ is compromised, the cell appears to use HR to repair the break, resulting in increased anaphase bridge formation. Moreover, intrinsic NHEJ activity of different cell lines appears to have a positive trend with induction of bridges from DNA damage. © 2007 Wiley-Liss, Inc. [source] | ||||||||||||||||||||||||||||||||||||||||||||||