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I Band (i + band)
Kinds of I Band Selected AbstractsPolarized Raman microspectroscopy on intact human hairJOURNAL OF BIOPHOTONICS, Issue 5 2008K. R. Ackermann Abstract Polarization-resolved Raman microspectroscopy with near-infrared laser excitation was applied to intact human hair in order to non-invasively investigate the conformation and orientation of the polypeptide chains. By varying the orientation of the hair shaft relative to the polarization directions of the laser/analyzer, a set of four polarized Raman spectra is obtained; this allows to simultaneously determine both the secondary structure of hair proteins and the orientation of the polypeptide strands relative to the axis of the hair shaft. For the amide I band, results from a quantitative analysis of the polarized Raman spectra are compared with theoretically expected values for fibers with uniaxial symmetry. Based on the polarization behavior of the amide I band and further vibrational bands, a partial ordering of ,-helical polypeptide strands parallel to the hair shaft can be concluded. We suggest that this microspectroscopic approach may be used for human hair diagnostics by detecting structural or orientational alterations of keratins. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] The SAURON project , IV.MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2006The mass-to-light ratio, lenticular galaxies, the Fundamental Plane of elliptical, the virial mass estimator ABSTRACT We investigate the well-known correlations between the dynamical mass-to-light ratio (M/L) and other global observables of elliptical (E) and lenticular (S0) galaxies. We construct two-integral Jeans and three-integral Schwarzschild dynamical models for a sample of 25 E/S0 galaxies with SAURON integral-field stellar kinematics to about one effective (half-light) radius Re. They have well-calibrated I -band Hubble Space Telescope WFPC2 and large-field ground-based photometry, accurate surface brightness fluctuation distances, and their observed kinematics is consistent with an axisymmetric intrinsic shape. All these factors result in an unprecedented accuracy in the M/L measurements. We find a tight correlation of the form (M/L) = (3.80 ± 0.14) × (,e/200 km s,1)0.84±0.07 between the M/L (in the I band) measured from the dynamical models and the luminosity-weighted second moment ,e of the LOSVD within Re. The observed rms scatter in M/L for our sample is 18 per cent, while the inferred intrinsic scatter is ,13 per cent. The (M/L),,e relation can be included in the remarkable series of tight correlations between ,e and other galaxy global observables. The comparison of the observed correlations with the predictions of the Fundamental Plane (FP), and with simple virial estimates, shows that the ,tilt' of the FP of early-type galaxies, describing the deviation of the FP from the virial relation, is almost exclusively due to a real M/L variation, while structural and orbital non-homology have a negligible effect. When the photometric parameters are determined in the ,classic' way, using growth curves, and the ,e is measured in a large aperture, the virial mass appears to be a reliable estimator of the mass in the central regions of galaxies, and can be safely used where more ,expensive' models are not feasible (e.g. in high-redshift studies). In this case the best-fitting virial relation has the form (M/L)vir= (5.0 ± 0.1) ×Re,2e/(LG), in reasonable agreement with simple theoretical predictions. We find no difference between the M/L of the galaxies in clusters and in the field. The comparison of the dynamical M/L with the (M/L)pop inferred from the analysis of the stellar population, indicates a median dark matter fraction in early-type galaxies of ,30 per cent of the total mass inside one Re, in broad agreement with previous studies, and it also shows that the stellar initial mass function varies little among different galaxies. Our results suggest a variation in M/L at constant (M/L)pop, which seems to be linked to the galaxy dynamics. We speculate that fast-rotating galaxies have lower dark matter fractions than the slow-rotating and generally more-massive ones. If correct, this would suggest a connection between the galaxy assembly history and the dark matter halo structure. The tightness of our correlation provides some evidence against cuspy nuclear dark matter profiles in galaxies. [source] Stellar populations and surface brightness fluctuations: new observations and modelsMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2001John P. Blakeslee We investigate the use of surface brightness fluctuations (SBF) measurements in optical and near-IR bandpasses for both stellar population and distance studies. New V -band SBF data are reported for five galaxies in the Fornax cluster and combined with literature data to define a V -band SBF distance indicator, calibrated against Cepheid distances to the Leo group and the Virgo and Fornax clusters. The colour dependence of the V -band SBF indicator is only ,15 per cent steeper than that found for the I band, and the mean ,fluctuation colour' of the galaxies is We use new stellar population models, based on the latest Padua isochrones transformed empirically to the observational plane, to predict optical and near-IR SBF magnitudes and integrated colours for a wide range of population ages and metallicities. We examine the sensitivity of the predicted SBF,colour relations to changes in the isochrones, stellar transformations, and initial mass function. The new models reproduce fairly well the weak dependence of V and I SBF in globular clusters on metallicity, especially if the more metal-rich globulars are younger. Below solar metallicity, the near-IR SBF magnitudes depend mainly on age, while the integrated colours depend mainly on metallicity. This could prove a powerful new approach to the age,metallicity degeneracy problem; near-IR SBF observations of globular clusters would be an important test of the models. The models also help in understanding the and fluctuation colours of elliptical galaxies, with much less need for composite stellar populations than in previous models. However, in order to obtain theoretical calibrations of the SBF distance indicators, we combine the homogeneous population models into composite models and select out those ones with fluctuation colours consistent with observations. We are able to reproduce the observed range of elliptical galaxy colours, the slopes of the V and I SBF distance indicators against (fainter SBF in redder populations), and the flattening of the I -band relation for The models also match the observed slope of I -band SBF against the Mg2 absorption index and explain the steep colour dependence found by Ajhar et al. for the HST/WFPC2 F814W-band SBF measurements. In contrast to previous models, ours predict that the near-IR SBF magnitudes will also continue to grow fainter for redder populations. The theoretical V -band SBF zero-point predicted by these models agrees well with the Cepheid-calibrated V -band empirical zero-point. However, the model zero-point is 0.15,0.27 mag too faint in the I band and 0.24,0.36 mag too faint in K. The zero-points for the I band (empirically the best determined) would come into close agreement if the Cepheid distance scale were revised to agree with the recent dynamical distance measured to NGC 4258. We note that the theoretical SBF calibrations are sensitive to the uncertain details of stellar evolution, and conclude that the empirical calibrations remain more secure. However, the sensitivity of SBF to these finer details potentially makes it a powerful, relatively unexploited, constraint for stellar evolution and population synthesis. [source] Changes in the Room-temperature Emission Spectrum of Chlorophyll During Fast and Slow Phases of the Kautsky Effect in Intact Leaves,PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 2 2005Fabrice Franck ABSTRACT Changes in the room-temperature emission spectrum of chlorophyll (Chl) were analyzed using fast diode-array recordings during the Kautsky effect in mature and in greening barley leaves. In mature leaves, the comparison of Fo (basal level of fluorescence yield at transient O) and FM (maximum level of fluorescence yield at transient M) spectra showed that the relative amplitude of total variable fluorescence was maximal for the 684 nm Photosystem II (PSII) band and minimal for the 725 nm Photosystem I band. During the increase from Fo to FM a progressive redshift of the spectrum of variable fluorescence occurred. This shift reflected the different fluorescence rise kinetics of different layers of chloroplasts inside the leaf. This was verified by simulating the effect of screening on the emission spectrum of isolated chloroplasts and by experiments on greening leaves with low Chl content. In addition, experiments performed at different greening stages showed that the presence of uncoupled Chl at early-greening stages and lightharvesting complex II (LHCII) at later stages have detectable but minor effects on the shape of room-temperature emission spectra. When strong actinic light was applied to mature green leaves, the slow fluorescence yield, which declined from FM to FT (steady-state level of fluorescence yield at transient T), was accompanied by a slight redshift of the 684 nm PSII band because of nonphotochemical quenching of short-wavelengthemitting Chl ascribed to LHCII. [source] Identifications of FIRST radio sources in the NOAO Deep Wide-Field SurveyASTRONOMISCHE NACHRICHTEN, Issue 6 2007K. El Bouchefry Abstract In this paper we present the results of an optical and near infrared identification of 514 radio sources from the FIRST survey (Faint Images of the Radio Sky Survey at Twenty centimetres) with a flux-density limit of 1 mJy in the NOAO Deep Wide-Field Survey (NDWFS) Boötes field. Using optical (Bw, R, I) and K band data with approximate limits of Bw , 25.5 mag, R , 25.8 mag, I , 25.5 mag and K , 19.4 mag, optical counterparts have been identified for 378 of 514 FIRST radio sources. This corresponds to an identification rate of 34% in four bands (BwRIK), 60% in optical bands (BwRI) and 74% in the I band. Photometric redshifts for these sources have been computed using the hyperz code. The inclusion of quasar template spectra in hyperz is investigated. We note that the photometric data are, in many cases, best matched to templates with very short star-formation timescales and the inferred ages of identified galaxies depend strongly on the assumptions about the star-formation timescale. The redshifts obtained are fairly consistent with those expected from the K - z relation for brighter radio sources but there is more scatter in the K - z diagram at z < 1. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] A rapid method for assessing lipid:protein and detergent:protein ratios in membrane-protein crystallizationACTA CRYSTALLOGRAPHICA SECTION D, Issue 1 2003Corrie J. B. DaCosta A simple procedure for rapidly measuring lipid:protein ratios and detergent concentrations at different stages of the solubilization, purification and crystallization of membrane proteins has been developed. Fourier-transform infrared spectra recorded from 10,µl aliquots of solution using a single-bounce diamond-attenuated total reflectance apparatus exhibit characteristic bands arising from the vibrations of lipid, protein and detergent. Lipid:protein molar ratios as low as 5:1 (for a protein with a molecular weight of 300,kDa) are determined by comparing the ratio of the integrated intensity of the lipid ester carbonyl band near 1740,cm,1 with the protein amide I band near 1650,cm,1. Detergent concentrations at levels well below the critical micellar concentration of most detergents are determined by comparing the integrated intensities of the detergent vibrations, particularly in the 1200,1000,cm,1 region, with a standard curve. Protein amide I band-shape analysis provides insight into the effects of detergents on protein secondary structure. The importance of monitoring detergent concentration changes during simple procedures, such as the concentration of a membrane protein by ultrafiltration, is demonstrated. This analytical tool has been used to rapidly establish protocols for minimizing lipid and detergent levels in solubilized membrane-protein samples. [source] Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopyBIOELECTROMAGNETICS, Issue 7 2003Toshitaka Ikehara Abstract The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorbance of the amide II band, and increased absorbance at around 1600 cm,1. These results suggest that exposure to the ELF magnetic field has reversible effects on the N,H inplane bending and C,N stretching vibrations of peptide linkages, and changes the secondary structures of ,-helix and ,-sheet in cell membrane proteins. Bioelectromagnetics 24:457,464, 2003. © 2003 Wiley-Liss, Inc. [source] Spectroscopic study on structure of horseradish peroxidase in water and dimethyl sulfoxide mixtureBIOPOLYMERS, Issue 2 2002Yasushi Maeda Abstract The structure of horseradish peroxidase (HRP) in phosphate buffered saline (PBS)/dimethyl sulfoxide (DMSO) mixed solvents at different compositions is investigated by IR, electronic absorption, and fluorescence spectroscopies. The fluorescence spectra and the amide I spectra of ferric HRP [HRP(Fe3+)] show that overall structural changes are relatively small up to 60% DMSO. Although the amide I band of HRP(Fe3+) shows a gradual change in the secondary structure and a decrease in the contents of , helices, its fluorescence spectra indicate that the distance between the heme and Trp173 is almost constant. In contrast, the changes in the positions of the Soret bands for resting HRP(Fe3+) and catalytic intermediates (compounds I and II) and the IR spectra at the CO stretching vibration mode of carbonyl ferrous HRP [HRP(Fe2+)-CO] show that the microenvironment in the distal heme pocket is altered, even with low DMSO contents. The large reduction of the catalytic activity of HRP even at low DMSO contents can be attributed to the structural transition in the distal heme pocket. In PBS/DMSO mixtures containing more than 70 vol % DMSO, HRP undergoes large structural changes, including a large loss of the secondary structure and a dissociation of the heme from the apoprotein. The presence of the components of the amide I band that can be assigned to strongly hydrogen bonding amide CO groups at 1616 and 1684 cm,1 suggests that the denatured HRP may aggregate through strong hydrogen bonds. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 107,112, 2002 [source] New Fourier transform infrared based computational method for peptide secondary structure determination.BIOPOLYMERS, Issue 2 2001Abstract Fourier transform infrared (FTIR) experiments in dimethylsulfoxide, a solvent incapable of H donation, demonstrate that H , D isotopic replacement on the amide side of peptide bonds involves modifications of both the position and intensity of the amide I band. The effect of the isotopic substitution is particularly significant in the 1710,1670 and 1670,1650 cm,1 regions, which are generally associated with ,-turns and ,-helices. This behavior, attributed to the existence of intramolecular H-bonds in the polypeptide chain, is directly correlated to the presence of different secondary structures. Utilizing the effects induced by isotopic substitution, a method for the quantitative determination of the percentage of intramolecular H-bonds and the correlated secondary structures is proposed. The method consists of three principal steps: resolution of the fine structure of the amide I band with the determination of the number and position of the different components; reconstruction of the experimentally measured amide I band as a combination of Gaussian and Lorentzian functions, centered on the wave numbers set by band-narrowing methods, through a curve-fitting program; and quantitative determination of the population of the H-bonded carbonyls and the correlated secondary structures by comparison of the integrated intensities pertaining to the components with homologous wave numbers before and after isotopic exchange. The method is tested on a synthetic fragment of proocytocin that was previously analyzed by NMR techniques using the same solvent systems. © 2001 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 62: 95,108, 2001 [source] Rapid monitoring of recombinant antibody production by mammalian cell cultures using fourier transform infrared spectroscopy and chemometricsBIOTECHNOLOGY & BIOENGINEERING, Issue 3 2010Christopher A. Sellick Abstract Fourier transform infrared (FT-IR) spectroscopy combined with multivariate statistical analyses was investigated as a physicochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) and murine myeloma non-secreting 0 (NS0) cell lines. Medium samples were taken during culture of CHO and NS0 cells lines, which included both antibody-producing and non-producing cell lines, and analyzed by FT-IR spectroscopy. Principal components analysis (PCA) alone, and combined with discriminant function analysis (PC-DFA), were applied to normalized FT-IR spectroscopy datasets and showed a linear trend with respect to recombinant protein production. Loadings plots of the most significant spectral components showed a decrease in the C,O stretch from polysaccharides and an increase in the amide I band during culture, respectively, indicating a decrease in sugar concentration and an increase in protein concentration in the medium. Partial least squares regression (PLSR) analysis was used to predict antibody titers, and these regression models were able to predict antibody titers accurately with low error when compared to ELISA data. PLSR was also able to predict glucose and lactate amounts in the medium samples accurately. This work demonstrates that FT-IR spectroscopy has great potential as a tool for monitoring cell cultures for recombinant protein production and offers a starting point for the application of spectroscopic techniques for the on-line measurement of antibody production in industrial scale bioreactors. Biotechnol. Bioeng. 2010; 106: 432,442. © 2010 Wiley Periodicals, Inc. [source] | ||||||||||||||||