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Hybridization Properties (hybridization + property)
Selected AbstractsC5-Functionalized LNA: Unparalleled Hybridization Properties and Enzymatic StabilityCHEMBIOCHEM, Issue 17 2009Michael E. Østergaard The best of both worlds: C5-functionalized locked nucleic acids (LNAs) synergistically combine the beneficial features of C5-alkynyl DNAs and conventional antisense LNA,they are straightforward to make and yet exhibit unparalleled thermal affinity toward RNA targets, extraordinary discrimination of singly mismatched RNA strands, and superb stability against degradation by 3,-exonucleases. [source] 4-Guanidino-2-pyrimidinone Nucleobases: Synthesis and Hybridization PropertiesCHEMINFORM, Issue 6 2004J. Robles Abstract For Abstract see ChemInform Abstract in Full Text. [source] Synthesis and Hybridization Properties of Modified Oligodeoxynucleotides Carrying Non-Natural BasesCHEMISTRY & BIODIVERSITY, Issue 2 2009Anna Aviñó Abstract The impact of the presence of nonnatural bases on the properties of oligodeoxynucleotides has been studied. First, oligodeoxynucleotides carrying 2,-deoxyzebularine were prepared, and the stability of duplexes carrying this analogue was determined by DNA melting experiments. Melting temperatures and thermodynamic data indicated the preference of 2,-deoxyzebularine for 2,-deoxyguanosine, which behaves as a 2,-deoxycytidine analogue, forming a less stable base pair due to the absence of the amino group at position 4. Moreover, the duplex,hairpin equilibrium of a self-complementary oligodeoxynucleotide carrying several natural and nonnatural bases including 2,-deoxyzebularine as a central mispair, was studied. Depending on the base present in the middle of the sequence, it is possible to affect the stability of the bimolecular duplex modulating the duplex,hairpin equilibrium. Magnesium ions were shown to stabilize preferentially the bimolecular duplex form. The results indicate the importance of the modifications and the role of cations in shifting the structural equilibrium. [source] Synthesis, Functionalization, and Bioconjugation of Monodisperse, Silica-Coated Gold Nanoparticles: Robust Bioprobes,ADVANCED FUNCTIONAL MATERIALS, Issue 6 2005S. H. Liu Abstract Herein, we report an efficient process for preparing monodisperse Au@SiO2 nanoparticles using homogeneous shaking and without the use of surface-coupling silane agents or large stabilizers. The resulting pure-silica surface of the Au@SiO2 nanoparticles is very important for straightforward surface functionalization with different functional groups via well-established silica surface chemistry. Subsequent covalent bioconjugation of the aldehyde-functionalized Au@SiO2 nanoparticles with various biomolecules is successfully employed to make robust nanoprobes for fast, colorimetric DNA and protein detection based on the sequence-specific hybridization properties of DNA and the specific binding affinity between proteins, respectively. [source] Structural compatibility of novel nucleotide modifications with shortened linkages designed for antigene/antisense therapyJOURNAL OF RAMAN SPECTROSCOPY, Issue 5 2004J. Hanu Abstract The impact of isopolar shortened internucleotide linkage modification on the hybridization properties of potential antisense or antigene oligonucleotides was studied by using a model molecular system consisting of polyuridylic acid (PolyU) and analogs of diadenosine monophosphate with the modified linkage. Raman spectra of mixed aqueous solutions were measured at various temperatures and compositions of the mixtures for four types of modified linkage and natural ApA (3,,5,) as a reference. Analysis of the spectral sets provided amounts of formed complexes and their Raman spectra. It has been found that as in the case of ApA (3,,5,), the studied ApA analogs form with PolyU triplex-like complexes containing a central pseudo-chain of closely arranged adenosine dimers. In the case of S - and R -configured 2,,5, and 3,,5, ApCOH A analogs, respectively, the amounts of complexes formed even exceed the ApA. This hybridization effectiveness is reached, however, by a more feasible prolongation of the pseudo-chain, while the stability constant for the initiation step is significantly lower. Raman spectra of the complexes showed that for both of the above analogs the structural compatibility with the natural nucleic acid chain is decreased, because of the distorted position of one adenine residue. This distortion influences the Watson,Crick hydrogen bonds. The two types of linkages may be suitable just for construction of longer antigene or antisense oligonucleotides with alternating lengthened and shortened linkages. There is, however, a warning of possible decreased binding specificity. Copyright © 2004 John Wiley & Sons, Ltd. [source] | ||||||||||