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Hybrid Cells (hybrid + cell)

Distribution by Scientific Domains
Medical Sciences45%
Life Sciences18%

Terms modified by Hybrid Cells

  • hybrid cell line
    		•

    Selected Abstracts

    Photovoltaics literature survey (No. 70)

    PROGRESS IN PHOTOVOLTAICS: RESEARCH & APPLICATIONS, Issue 4 2009
    Avi Shalav
    A single-source reference to the latest solar PV literature, each issue of Progress in Photovoltaics captures the most recently published relevant articles from a wide range of engineering, physics and materials science journals, presented in the following broad categories: 1. Fundamentals, new approaches, and reviews 2. General characterisation techniques and modelling 3. Crystalline silicon-bulk cells and technology 4. Thin film silicon, amorphous and micro/nano-crystalline silicon, heterojunction cells 5. Organic and Hybrid cells 6. Photoelectrochemical cells 7. CIS, CIGS, CdTe and II-VI cells 8. III-V, quantum well, space, concentrator and thermophotovoltaic cells 9. Terrestrial modules, BOS components, building integrated, systems and applications 10. Policy, economics, education, health, environment and the solar resource. [source]

    Photovoltaics literature survey (No. 69)

    PROGRESS IN PHOTOVOLTAICS: RESEARCH & APPLICATIONS, Issue 3 2009
    Avi Shalav
    A single-source reference to the latest solar PV literature, each issue of Progress in Photovoltaics captures the most recently published relevant articles from a wide range of engineering, physics and materials science journals, presented in the following broad categories: 1. Fundamentals, new approaches, and reviews 2. General characterisation techniques and modelling 3. Crystalline silicon-bulk cells and technology 4. Thin film silicon, amorphous and micro/nano-crystalline silicon, heterojunction cells 5. Organic and Hybrid cells 6. Photoelectrochemical cells 7. CIS, CIGS, CdTe and II-VI cells 8. III-V, quantum well, space, concentrator and thermophotovoltaic cells 9. Terrestrial modules, BOS components, building integrated, systems and applications 10. Policy, economics, education, health, environment and the solar resource. [source]

    Photovoltaics literature survey (No. 68)

    PROGRESS IN PHOTOVOLTAICS: RESEARCH & APPLICATIONS, Issue 2 2009
    Avi Shalav
    A single-source reference to the latest solar PV literature, each issue of Progress in Photovoltaics captures the most recently published relevant articles from a wide range of engineering, physics and materials science journals, presented in the following broad categories: 1. Fundamentals, new approaches, and reviews 2. General characterisation techniques and modelling 3. Crystalline silicon-bulk cells and technology 4. Thin film silicon, amorphous and micro/nano-crystalline silicon, heterojunction cells 5. Organic and Hybrid cells 6. Photoelectrochemical cells 7. CIS, CIGS, CdTe and II-VI cells 8. III-V, quantum well, space, concentrator and thermophotovoltaic cells 9. Terrestrial modules, BOS components, building integrated, systems and applications 10. Policy, economics, education, health, environment and the solar resource. [source]

    Preclinical development of hybrid cell vaccines for multiple myeloma

    EUROPEAN JOURNAL OF HAEMATOLOGY, Issue 1 2007
    Renata Walewska
    Abstract Immunotherapy may provide alternative or supplementary treatment of multiple myeloma (MM). We propose that hybrid cells, formed by fusing professional antigen-presenting cells with malignant plasma cells, would induce immune responses capable of mediating tumour regression. The human B-lymphoblastoid cell line, HMy2, was fused in vitro with CD138+ bead-separated myeloma plasma cells from five patients with MM. The hybrid cell lines generated in these studies grew stably in tissue culture, and maintained their phenotypic and functional characteristics, providing self-renewing cell lines with potential for therapeutic vaccination. The hybrid cells stimulated allogeneic and autologous T-cell proliferative responses in vitro to a considerably greater degree than their respective parent myeloma plasma cells, and directly activated both CD4+ and CD8+ T-cell responses. The enhanced T-cell stimulation correlated with expression of CD80 on the hybrid cells, and was inhibited by CTLA4-Ig fusion protein. The hybrid cell lines expressed several tumour-associated antigens known to be expressed in myeloma. These data show that self-replicating cell lines with enhanced immunostimulatory properties and potential for therapeutic vaccination can be generated by in vitro fusion of ex vivo myeloma cells and B-lymphoblastoid cell lines. [source]

    Characterization and gene expression profiling in glioma cell lines with deletion of chromosome 19 before and after microcell-mediated restoration of normal human chromosome 19

    GENES, CHROMOSOMES AND CANCER, Issue 10 2009
    Kristen L. Drucker
    Nearly 10% of human gliomas are oligodendrogliomas. Deletion of chromosome arm 19q, often in conjunction with deletion of 1p, has been observed in 65,80% of these tumors. This has suggested the presence of a tumor suppressor gene located on the 19q arm. Chromosome 19 deletion is also of interest due to the better prognosis of patients with deletion, including longer survival and better response to chemotherapy, compared with patients without deletion. Two glioma cell lines with deletion of 19q were used for chromosome 19 microcell-mediated transfer, to assess the effect of replacing the deleted segment. Complementation with chromosome 19 significantly reduced the growth rate of the hybrid cells compared with the parental cell lines. Affymetrix U133 Plus 2.0 Gene Chip analysis was performed to measure and compare the expression of the chromosome 19 genes in the chromosome 19 hybrid cell lines to the parental cell line. Probes were considered significantly different when a P value <0.01 was seen in all of the cell line comparisons. Of 345 probes within the commonly deleted 19q region, seven genes (APOE, RCN3, FLJ10781, SAE1, STRN4, CCDC8, and BCL2L12) were identified as potential candidate genes. RT-PCR analysis of primary tumor specimens showed that several genes had significant differences when stratified by tumor morphology or deletion status. This suggests that one or more of these candidates may play a role in glioma formation or progression. © 2009 Wiley-Liss, Inc. [source]

    Homozygous deletions within the 11q13 cervical cancer tumor-suppressor locus in radiation-induced, neoplastically transformed human hybrid cells

    GENES, CHROMOSOMES AND CANCER, Issue 4 2004
    Marc S. Mendonca
    Studies on nontumorigenic and tumorigenic human cell hybrids derived from the fusion of HeLa (a cervical cancer cell line) with GM00077 (a normal skin fibroblast cell line) have demonstrated "functional" tumor-suppressor activity on chromosome 11. It has been shown that several of the neoplastically transformed radiation-induced hybrid cells called GIMs (gamma ray induced mutants), isolated from the nontumorigenic CGL1 cells, have lost one copy of the fibroblast chromosome 11. We hypothesized, therefore, that the remaining copy of the gene might be mutated in the cytogenetically intact copy of fibroblast chromosome 11. Because a cervical cancer tumor suppressor locus has been localized to chromosome band 11q13, we performed deletion-mapping analysis of eight different GIMs using a total of 32 different polymorphic and microsatellite markers on the long arm (q arm) of chromosome 11. Four irradiated, nontumorigenic hybrid cell lines, called CONs, were also analyzed. Allelic deletion was ascertained by the loss of a fibroblast allele in the hybrid cell lines. The analysis confirmed the loss of a fibroblast chromosome 11 in five of the GIMs. Further, homozygous deletion (complete loss) of chromosome band 11q13 band sequences, including that of D11S913, was observed in two of the GIMs. Detailed mapping with genomic sequences localized the homozygous deletion to a 5.7-kb interval between EST AW167735 and EST F05086. Southern blot hybridization using genomic DNA probes from the D11S913 locus confirmed the existence of homozygous deletion in the two GIM cell lines. Additionally, PCR analysis showed a reduction in signal intensity for a marker mapped 31 kb centromeric of D11S913 in four other GIMs. Finally, Northern blot hybridization with the genomic probes revealed the presence of a novel >15-kb transcript in six of the GIMs. These transcripts were not observed in the nontumorigenic hybrid cell lines. Because the chromosome 11q13 band deletions in the tumorigenic hybrid cell lines overlapped with the minimal deletion in cervical cancer, the data suggest that the same gene may be involved in the development of cervical cancer and in radiation-induced carcinogenesis. We propose that a gene localized in proximity to the homozygous deletion is the candidate tumor-suppressor gene. © 2004 Wiley-Liss, Inc. [source]

    Endotoxin-stimulated macrophages decrease bile acid uptake in WIF-B cells, a rat hepatoma hybrid cell line

    HEPATOLOGY, Issue 1 2000
    Ekkehard Sturm
    Endotoxemia leads to cytokine-mediated alterations of the hepatocellular sodium-taurocholate-cotransporting polypeptide (ntcp). We hypothesized that stimulated macrophages are essential transducers for down-regulating hepatocellular bile salt uptake in response to endotoxin (lipopolysaccharide [LPS]) exposure. Using an in vitro model, we exposed mouse macrophages (IC-21 cell line) to LPS for 24 hours. Concentrations of cytokines tumor necrosis factor-, (TNF-,), interleukin (IL)-1,, and IL-6 increased 10.6-fold, 12.5-fold, and 444-fold, respectively, in LPS-conditioned IC-21 medium (CM) versus unconditioned IC-21 medium (UM). WIF-B rat hepatoma hybrid cells were incubated with either CM or UM or treated directly with medium containing recombinant TNF-,, IL-1,, and IL-6. [3H]Taurocholate ([3H]TC) uptake decreased in WIF-B cells exposed to either TNF-, (54% of control), IL-1, (78%), IL-6 (55%) as single additives, or in triple combination (TCC) (43%). A virtually identical decrease was observed after exposing WIF-B cells to CM (52%, P < .001). LPS had no direct effect on [3H]TC uptake. CM treatment did not decrease L-alanine transport in WIF-B cells. Blocking antibodies against TNF-,, IL-1,, and IL-6 restored the diminished [3H]TC uptake in cells exposed to TCC and CM to 87% and 107% of controls, respectively. Northern blotting revealed that ntcp messenger RNA (mRNA) expression was significantly reduced in WIF-B cells after exposure to CM, and in primary rat hepatocytes exposed to CM or TNF-, (68%, 14%, and 29% of control, respectively). We conclude that macrophages and their ability to secrete the cytokines TNF-,, IL-1,, and IL-6 may be essential in mediating the endotoxin-induced cholestatic effect of decreased hepatocellular bile salt uptake. [source]

    Improved Photovoltaic Performance of Heterostructured Tetrapod-Shaped CdSe/CdTe Nanocrystals Using C60 Interlayer

    ADVANCED MATERIALS, Issue 44 2009
    Yanqin Li
    Photovoltaic (PV) devices based on CdSe/CdTe-C60 active layers, in which the nanocrystal and fullerene (C60) layers work as electron-donor and electron-acceptor/transport layers, respectively, were fabricated. Efficiencies up to 0.62% were reached in the hybrid cells. The PV performance was greatly improved with respect to that of CdSe/CdTe-P3HT- and CdSe/CdTe-based devices, fabricated as reference. [source]

    Binucleate trophoblast giant cells in the water buffalo (Bubalus bubalis) placenta

    JOURNAL OF MORPHOLOGY, Issue 1 2006
    A.F. Carvalho
    Abstract The binucleate trophoblast giant cells (BNC) of the water buffalo, Bubalus bubalis, placenta were studied, with emphasis on the synthesis of BNC-specific proteins. Placentomal tissues of 27 water buffalos (2,10 months of pregnancy) were processed for light and electron microscopy. The frequency of BNCs was 20% of the trophoblastic cells in 2,3-month placentas and increased to 27% in the later stages. Ultrastructurally, binucleate cells displayed a prominent granular endoplasmic reticulum and Golgi apparatus, typical of cells involved with protein synthesis and exportation. The buffalo BNCs contained periodic acid-Schiff (PAS)-positive granules and reacted with antisera against bovine placental lactogen, prolactin-related protein-I, and pregnancy-associated glycoproteins. Lectin histochemistry with Dolichos biflorus agglutinin, Vicia villosa agglutinin, and Phaseolus vulgaris leucoagglutinin showed specific staining of BNCs. Different stages of BNC migration and fusion with uterine epithelial cells were observed. Trinucleate feto-maternal hybrid cells were the typical outcome of cell fusions. These cells underwent degeneration, with typical morphological features of apoptosis. The results revealed a strong homology between water buffalo and cattle BNCs concerning cell morphology, protein expression, glycosylation pattern, and characteristics of cell migration and fusion. J. Morphol. © 2005 Wiley-Liss, Inc. [source]

    Hybrid Lethality in Interspecific F1 Hybrid Nicotiana gossei×N. tabacum Involves a MAP-Kinases Signalling Cascade

    PLANT BIOLOGY, Issue 3 2007
    M. Mino
    Abstract: A cultured cell line, GTH4 (Nicotiana gossei Domin ×N. tabacum L.), which exhibits hybrid lethality, died at 26 °C, but not at 37 °C. Pharmacological experiments using inhibitors of protein phosphatases and protein kinases indicated the involvement of a protein kinase signalling pathway in the cell death process. Immunoblot analysis revealed that salicylic acid-induced protein kinase (SIPK) was phosphorylated soon after the shift in temperature from 37 °C to 26 °C. Cultured cells of the hybrid of N. gossei× transgenic N. tabacum harboring a steroid (dexamethasone; DEX)-inducible NtMEK2DD or NtMEK2KR, constitutively active and inactive forms of NtMEK2, respectively, were established. Induction of NtMEK2DD by DEX in the hybrid cells induced the activation of SIPK, the generation of hydrogen peroxide (H2O2), and cell death at 37 °C. The activation of SIPK, generation of H2O2, and cell death at 26 °C were compromised by DEX treatment in hybrid cells harbouring NtMEK2KR. This study provides evidence for the involvement of MAPK signalling in the regulation of cell death in hybrids. [source]