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Human Faeces (human + faeces)
Selected AbstractsDesulfotomaculum genus- and subgenus-specific 16S rRNA hybridization probes for environmental studiesENVIRONMENTAL MICROBIOLOGY, Issue 2 2000Krassimira R. Hristova Based on comparative analysis of 16S rRNA sequences and the recently established phylogeny of the genus Desulfotomaculum, a set of phylogenetically nested hybridization probes was developed and characterized. A genus-specific probe targets all known Desulfotomaculum species (with the exception of Desulfotomaculum acetoxidans,), and five specific probes target subclusters within the Desulfotomaculum genus. The dissociation temperature of each probe was determined experimentally. Probe specificities were verified through hybridizations with pure culture rRNA isolated from a wide variety of target and non-target organisms and through an evaluation of probe ,nesting' using samples obtained from four different environments. Fixation and hybridization conditions for fluorescence in situ hybridizations were also optimized. The probes were used in quantitative membrane hybridizations to determine the abundance of Desulfotomaculum species in thermophilic anaerobic digesters, in soil, in human faeces and in pig colon samples. Desulfotomaculum rRNA accounted for 0.3,2.1% of the total rRNA in the digesters, 2.6,6.6% in soil, 1.5,3.3% in human faeces and 2.5,6.2% in pig colon samples. [source] Helicobacter equorum: prevalence and significance for horses and humansFEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 1 2009Hilde Moyaert Abstract Helicobacter equorum colonizes the caecum, colon and rectum of horses. The agent is highly prevalent in <6-month-old foals. In adult horses, the prevalence of H. equorum seems to be rather low, but these animals may harbour low, subdetectable numbers of this microorganism in their intestines. So far, no association could be made between the presence of H. equorum and clinical disease or intestinal lesions in adult horses. Further research is necessary to elucidate the pathogenic potential of this bacterial species towards young foals. Helicobacter equorum DNA was not detected in human faeces, indicating that this microorganism does not commonly spread from horses towards humans. [source] The amount of secreted IgA may not determine the secretory IgA coating ratio of gastrointestinal bacteriaFEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 2 2009Takeshi Tsuruta Abstract It is reported that some, but not all, bacteria in human faeces are coated with secretory immunoglobulin A (S-IgA). We evaluated the proportion of S-IgA-coated bacteria to total intestinal bacteria (S-IgA coating ratio) in the gastrointestinal tract of two different strains of mice supplied by two different suppliers. The S-IgA coating ratio was significantly different in each gastrointestinal segment and between mouse suppliers. The amount of non-bacteria-bound IgA (free IgA) in each gastrointestinal segment indicated that this difference in the S-IgA coating ratio might not be due to the amount of secreted IgA. Furthermore, immunoblotting analysis revealed that only a small amount of IgA (<5% to free-IgA) was used for the coating. This indicates that, although sufficient S-IgA was secreted to coat the entire intestinal population of bacteria, only some part of the bacteria were coated with S-IgA. This study suggests that the amount of luminal S-IgA may not determine the S-IgA coating ratio, and that the amount of IgA coating intestinal commensal bacteria is very small. [source] Effect of bile on the lipid composition and surface properties of bifidobacteriaJOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2002A. Gómez Zavaglia Aim: The changes produced on the bacterial surface of Bifidobacteria cells when they are grown in bile were compared with those provoked by bile added to bacteria grown in the absence of bile. Methods and Results: The adhesive properties, the zeta potential and the lipid composition of Bifidobacterial strains, isolated from human faeces and grown in MRS medium, were determined. Bacteria grown in MRS with bile showed a loss of adherence and autoaggregation in correlation with a decrease in the surface hydrophobicity in comparison to those grown in MRS without bile, concomitant with the absence of two glycolipids, the increase of sugar content and minor changes in fatty acid composition. The surface changes caused by bile shock on bacteria grown in bile-free medium were much less pronounced and, in addition, no effect on the lipid composition was apparent. Conclusions: The comparison of the results indicates that bile action on surface properties is related to metabolic changes. Significance and Impact of the Study: Long-term exposure of bacteria to bile may cause metabolic changes affecting their adhesive properties irreversibly. This may be taken as a criterion to define the probiotic properties of different strains. [source] A colony immunoblotting method for quantitative detection of a Bifidobacterium animalis probiotic strain in human faecesJOURNAL OF APPLIED MICROBIOLOGY, Issue 6 2000H. Duez A colony immunoblotting method has been developed to allow detection of the probiotic Bifidobacterium animalis strain DN-173 010 in human faecal samples. Rabbits were immunized with heat-killed DN-173 010 bacteria resulting in the production of an antiserum highly specific for bacteria belonging to Bif. animalis species. Of the 89 strains representative of 29 different bifidobacterial species tested, only the 15 strains of the Bif. animalis species could be detected with the antiserum. In Western immunoblotting the serum reacts with a protein of 45-kDa apparent molecular weight. None of the bacteria classically encountered in human faecal samples and able to grow on non-selective Columbia blood agar (enterobacteria, Bacteroides or Lactobacillus for instance) reacted with the antiserum. Taking advantage of the high specificity of the antiserum and of the absence of Bif. animalis bacteria in faeces samples of five human volunteers, we demonstrated that strain DN-173 010 survives the intestinal transit. Being based on a combination of semiselective cultivation and colony immunoblotting techniques, the method allowed detection of the Bif. animalis strain even when it represented only one thousandth of the total bifidobacterial population. [source] Competition between domestic dogs and Ethiopian wolf (Canis simensis) in the Bale Mountains National Park, EthiopiaAFRICAN JOURNAL OF ECOLOGY, Issue 2 2010A. Atickem Abstract The potential effects of the domestic dogs (Canis familiaris) on the Endangered Ethiopian wolf (Canis simensis) through exploitative and interference competition were studied in the Web Valley of Bale Mountains national park between November 2001 and February 2003. All dogs were owned in the study area and no feral dogs were reported or observed during the research period. The diet of domestic dogs was dominated by barley husks and human faeces which contributed 45% and 20.7% of the total 382 meals observed during focal watch observations. Analysis of dog faeces provided similar results with barley husks, human faeces and animal carcasses occurring in 86.8%, 21.4% and 19.4% of the 1200 faecal samples analysed. Both focal watch and faecal analyses revealed that rodents contributed only a very small proportion of the diet of dogs accounting for only 4.2% of the focal watch and 2.8% of the faecal analysis of roaming dogs. As Ethiopian wolves fed almost exclusively on rodent year round, no significant exploitative competition between dogs and wolves were assessed. Only small proportion of the domestic dogs roamed in the Ethiopian wolf range and interference competition did not appear to be a serious threat for the Ethiopian wolf. Résumé De novembre 2001 à février 2003, on a étudié les effets potentiels des chiens domestiques (Canis familiaris) sur le loup d'Ethiopie (Canis simensis) qui est en danger, en raison d'une compétition par exploitation alimentaire ou par interférences, dans la Vallée de Web, dans le Parc National des montagnes de Bale. Tous les chiens de l'étude avaient un propriétaire, et nous n'avons ni rapporté ni observé de chien féral pendant cette période. Le régime alimentaire des chiens comprenait principalement de la balle d'orge et des excréments humains qui composaient respectivement 45% et 20,7% du total des 382 repas observés pendant des observations focalisées. L'analyse des excréments de chiens donne des résultats semblables : la balle d'orge, les excréments humains et les carcasses d'animaux sont présents dans 86,8%, 21,4% et 19,4% des 1 200 échantillons fécaux analysés. Et les observations focalisées et les analyses des crottes ont révélé que les rongeurs ne composent qu'une toute petite proportion du régime des chiens, avec 4,2% des observations et 2,8% des analyses fécales des chiens errant en liberté. Comme les loups d'Ethiopie se nourrissent toute l'année presque exclusivement de rongeurs, nous avons estimé qu'il n'y avait aucune compétition par exploitation significative entre les chiens et les loups. Seule une petite proportion de chiens circulent dans l'aire de distribution du loup d'Ethiopie, et la compétition par interférence a semblé ne pas constituer une menace sérieuse pour le loup d'Ethiopie. [source] The potential reuse of biodegradable municipal solid wastes (MSW) as feedstocks in vermicompostingJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 13 2010Edwin Yih Shyang Sim Abstract There is an urgent need globally to find alternative sustainable steps to treat municipal solid wastes (MSW) originated from mismanagement of urban wastes with increasing disposal cost. Furthermore, a conglomeration of ever-increasing population and consumerist lifestyle is contributing towards the generation of more MSW. In this context, vermicomposting offers excellent potential to promote safe, hygienic and sustainable management of biodegradable MSW. It has been demonstrated that, through vermicomposting, MSW such as city garbage, household and kitchen wastes, vegetable wastes, paper wastes, human faeces and others could be sustainably transformed into organic fertiliser or vermicompost that provides great benefits to agricultural soil and plants. Generally, earthworms are sensitive to their environment and require temperature, moisture content, pH and sometimes ventilation at proper levels for the optimum vermicomposting process. Apart from setting the optimum operational conditions for the vermicomposting process, other approaches such as pre-composting, inoculating micro-organisms into MSW and redesigning the conventional vermireactor could be introduced to further enhance the vermicomposting of MSW. Thus the present mini-review discusses the potential of introducing vermicomposting in MSW management, the benefits of vermicomposted MSW to plants, suggestions on how to enhance the vermicomposting of MSW as well as risk management in the vermicomposting of MSW. Copyright © 2010 Society of Chemical Industry [source] Human and other faeces as breeding media of the trachoma vector Musca sorbensMEDICAL AND VETERINARY ENTOMOLOGY, Issue 3 2001P. M. Emerson Abstract. The fly Musca sorbens Wiedemann (Diptera: Muscidae) apparently transmits Chlamydia trachomatis, causing human trachoma. The literature indicates that M. sorbens breeds predominantly in isolated human faeces on the soil surface, but not in covered pit latrines. We sought to identify breeding media of M. sorbens in a rural Gambian village endemic for trachoma. Test breeding media were presented for oviposition on soil-filled buckets and monitored for adult emergence. Musca sorbens emerged from human (6/9 trials), calf (3/9), cow (3/9), dog (2/9) and goat (1/9) faeces, but not from horse faeces, composting kitchen scraps or a soil control (0/9 of each). After adjusting for mass of medium, the greatest number of flies emerged from human faeces (1426 flies/kg). Median time for emergence was 9 (inter quartile range = 8,9.75) days post-oviposition. Of all flies emerging from faeces 81% were M. sorbens. Male and female flies emerging from human faeces were significantly larger than those from other media, suggesting that they would be more fecund and live longer than smaller flies from other sources. Female flies caught from children's eyes were of a similar size to those from human faeces, but significantly larger than those from other media. We consider that human faeces are the best larval medium for M. sorbens, although some breeding also occurs in animal faeces. Removal of human faeces from the environment, through the provision of basic sanitation, is likely to greatly reduce fly density, eye contact and hence trachoma transmission, but if faeces of other animals are present M. sorbens will persist. [source] | ||||||||||