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Human Decidua (human + decidua)

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Medical Sciences100%

Selected Abstracts

The Presence and Role of the Dopamine DA-2 Receptor in the Human Decidua

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (ELECTRONIC), Issue 6 2000
Fujimi Arai
Abstract Objectives: Our objectives were to identify the presence of the dopamine DA-2 receptor in human decidua and to study its function in human parturition. Methods: Human term decidual tissues were obtained during vaginal delivery and then homogenized. The P3 fraction was prepared for a radiolabeled receptor assay with [3H] spiperone as the ligand. Human decidual tissues obtained at cesarean section before the onset of labor were incubated in Krebs-Ringer buffer at 37°C for 30 minutes in the presence of dopamine with or without (,)-sulpiride. The level of prostaglandin (PG) F in the medium was measured with a RIA kit. Differences were assessed with the Wilcoxon non-parametric test. Results: Scatchard analysis showed a single class of binding sites having an equilibrium dissociation constant (Kd) of 2.25 + 0.59 nm (mean + SD) and a maximum binding capacity (Bmax) value of 166.5 + 77.7 fmol/mg protein (n = 3). Dopamine significantly increased the production of PGF. This stimulatory effect of dopamine was suppressed by (,)-sulpiride (p < 0.05; n = 7). Conclusion: The DA-2 receptor was demonstrated in the human decidua. Dopamine can stimulate PGF production via this receptor. [source]

Complete Compilation of Matrix Metalloproteinase Expression in Human Decidua during Pregnancy

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 3 2007
J Stojic
No abstract is available for this article. [source]

A Th2 Chemokine, TARC, Produced by Trophoblasts and Endometrial Gland Cells, Regulates the Infiltration of CCR4+ T Lymphocytes into Human Decidua at Early Pregnancy

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 1 2002
HIROSHI TSUDA
PROBLEM:,A chemokine receptor, CCR4 preferentially expressed on type 2 helper T (Th2-type) cells, and its ligand, thymus and activation regulated chemokine -(TARC/CCL)- play important roles in the recruitment of Th2-type cells. We examined the distribution of CCR4 expressing CD4+ and CD8+ -T cells in human decidua at early pregnancy, and localized TARC in the decidual tissue and chorionic tissue. METHOD OF STUDY:,Decidual tissue was obtained by legal abortion. The percentages of CCR4 expressing CD4+ and CD8+ -T cells were analyzed by flow cytometry. Localization of TARC protein was evaluated by immunofluorescence staining. The expression of TARC mRNA in the choriocarcinoma cell line and endometrial cell line was analyzed by reverse transcriptase polymerase chain reaction (RT,PCR). RESULT:,The percentages of CCR4+ cells in CD4+ -T cells and CD8+ -T cells were significantly increased in human early pregnancy decidua compared with those in peripheral blood. An another marker of human Th2 and Tc2 cells, CRTH2 molecules was also expressed on CCR4+CD4+ -T cells and CCR4+CD8+ -T cells. In addition, we found that trophoblasts, uterine epithelial cells and endometrial gland cells produce TARC by immunohistochemical staining and the RT-PCR method. CONCLUSION:,Our findings imply that TARC secreted in decidua mediates the infiltration of CCR4+ T-cell migration into the fetomaternal interface, decidua, resulting in the maintenance of pregnancy. [source]

The Presence and Role of the Dopamine DA-2 Receptor in the Human Decidua

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (ELECTRONIC), Issue 6 2000
Fujimi Arai
Abstract Objectives: Our objectives were to identify the presence of the dopamine DA-2 receptor in human decidua and to study its function in human parturition. Methods: Human term decidual tissues were obtained during vaginal delivery and then homogenized. The P3 fraction was prepared for a radiolabeled receptor assay with [3H] spiperone as the ligand. Human decidual tissues obtained at cesarean section before the onset of labor were incubated in Krebs-Ringer buffer at 37°C for 30 minutes in the presence of dopamine with or without (,)-sulpiride. The level of prostaglandin (PG) F in the medium was measured with a RIA kit. Differences were assessed with the Wilcoxon non-parametric test. Results: Scatchard analysis showed a single class of binding sites having an equilibrium dissociation constant (Kd) of 2.25 + 0.59 nm (mean + SD) and a maximum binding capacity (Bmax) value of 166.5 + 77.7 fmol/mg protein (n = 3). Dopamine significantly increased the production of PGF. This stimulatory effect of dopamine was suppressed by (,)-sulpiride (p < 0.05; n = 7). Conclusion: The DA-2 receptor was demonstrated in the human decidua. Dopamine can stimulate PGF production via this receptor. [source]

A Th2 Chemokine, TARC, Produced by Trophoblasts and Endometrial Gland Cells, Regulates the Infiltration of CCR4+ T Lymphocytes into Human Decidua at Early Pregnancy

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 1 2002
HIROSHI TSUDA
PROBLEM:,A chemokine receptor, CCR4 preferentially expressed on type 2 helper T (Th2-type) cells, and its ligand, thymus and activation regulated chemokine -(TARC/CCL)- play important roles in the recruitment of Th2-type cells. We examined the distribution of CCR4 expressing CD4+ and CD8+ -T cells in human decidua at early pregnancy, and localized TARC in the decidual tissue and chorionic tissue. METHOD OF STUDY:,Decidual tissue was obtained by legal abortion. The percentages of CCR4 expressing CD4+ and CD8+ -T cells were analyzed by flow cytometry. Localization of TARC protein was evaluated by immunofluorescence staining. The expression of TARC mRNA in the choriocarcinoma cell line and endometrial cell line was analyzed by reverse transcriptase polymerase chain reaction (RT,PCR). RESULT:,The percentages of CCR4+ cells in CD4+ -T cells and CD8+ -T cells were significantly increased in human early pregnancy decidua compared with those in peripheral blood. An another marker of human Th2 and Tc2 cells, CRTH2 molecules was also expressed on CCR4+CD4+ -T cells and CCR4+CD8+ -T cells. In addition, we found that trophoblasts, uterine epithelial cells and endometrial gland cells produce TARC by immunohistochemical staining and the RT-PCR method. CONCLUSION:,Our findings imply that TARC secreted in decidua mediates the infiltration of CCR4+ T-cell migration into the fetomaternal interface, decidua, resulting in the maintenance of pregnancy. [source]

Human First-Trimester Decidua Vascular Density: An Immunohistochemical Study Using VE-Cadherin and Endoglin as Endothelial Cell Markers

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 1 2000
BRUNO VAILHÉ
PROBLEM: Angiogenesis and vasculogenesis appear to be of critical importance for the success of pregnancy. Recent data have emphasized that pregnancy complications, such as abortion or pre-eclampsia, are linked with vascular pathologies. The aim of this study was to quantify human first-trimester decidua microvascular density, using two novel, highly specific endothelial cell markers, VE-cadherin and endoglin. METHOD OF STUDY: We collected decidua from women undergoing termination of normal pregnancies. VE-cadherin and endoglin were localized by immunohistochemistry. The blood vessel densities detected by VE-cadherin or endoglin-stainings were microscopically quantified per mm2. RESULTS: Endothelial cells in first-trimester human decidua both express VE-cadherin and endoglin. The microvascular density detected by VE-cadherin-staining varied from 32.2±1.7 in decidua basalis, to 30±0.6 in decidua parietalis. For the endoglin-staining, the values varied from 37.5±3 in decidua basalis, and 26.7±1.2 in decidua parietalis. CONCLUSIONS: Our data shows that both VE-cadherin and endoglin are good candidates to highlight the decidual endothelial cells, and to quantify the blood vessels density of endometrium. [source]

Inhibition of term decidual NK cell cytotoxicity by soluble HLA-G1

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 5-6 2006
Tobias G. Poehlmann
Objectives, Soluble (s)HLA-G1 is produced by trophoblast cells. Aim was to analyze the capacities and mechanisms of sHLA-G1 to regulate interleukin (IL)-2-induced cytotoxicity of natural killer (NK) cells from human deciduas. Methods, Natural killer cells were isolated from decidual layers of term placentae, stimulated or not with IL-2 and supplemented with various concentrations of recombinant soluble HLA-G1 (sHLA-G1). For NK cell cytotoxicity assays, K562 cells were used as targets. Expression of signal transducer and activator of transcription 3 (STAT3) and perforin was analyzed by Western blotting. Apoptosis was examined by assessment of poly(ADP-ribose) polymerase cleavage. NK cells were analyzed by flow cytometry for IL-2receptor- , (IL-2R,; CD25) and transferrin receptor CD71 expression. Results, Interleukin-2 increases CD71, STAT3, perforin expression and cytotoxic potential of NK cells. Expression of CD71, STAT3 and perforin decreased simultaneously with cytotoxicity and dose-dependently when sHLA-G1 (1.6 ,g/mL,1.6 ng/mL) was added to IL-2 stimulated cultures. sHLA-G1 did not induce apoptosis and CD25 expression was not affected. Conclusion, Interleukin-2R, expression is not controlled by sHLA-G1, but its signal transducer STAT3 as well as several downstream effects, such as perforin expression, proliferation and cytotoxicity. The control of STAT3 bioavailability through sHLA-G1 may be a key regulator of the mentioned effects. [source]