Home About us Contact
|
Home About us Contact | ||
|
|
||
Humulus Lupulus L. (humulus + lupulu_l)
Selected AbstractsDetermination of xanthohumol in hops (Humulus lupulus L.) by nonaqueous CEELECTROPHORESIS, Issue 6 2007Javor Kac Dr. Abstract Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5,cm long fused-silica capillary with 50,,m id at 25°C; 30,kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75,mM NaOH and 50,mM boric acid in methanol; hydrodynamical injection with 10,mbar for 40,s; and detection at 440,nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n -lupulone were well resolved on the electropherogram. The LOD for XN was 0.05,mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42%. [source] Isolation and characterization of polymorphic microsatellites for assessment of genetic variation of hops (Humulus lupulus L.)MOLECULAR ECOLOGY RESOURCES, Issue 2 2004A. M. Hadonou Abstract Hop (Humulus lupulus L.) cultivars are vegetatively propagated and it is difficult to differentiate them during the process of propagation. Fingerprinting with molecular markers based on DNA could be a useful means of identifying different cultivars. Simple sequence repeats, or microsatellite markers, are the most suitable marker for genetic fingerprinting because they are multi-allelic and co-dominant. For this purpose, we have developed primers for 10 new polymorphic microsatellite loci that are suitable for genetic fingerprinting in hop. [source] Eleven new microsatellites for hop (Humulus lupulus L.)MOLECULAR ECOLOGY RESOURCES, Issue 4 2002J. Jak Abstract We present a new set of 11 polymorphic microsatellite primer sequences for use with Humulus lupulus. Microsatellite-enriched libraries for GAn and GTn types of repeats were produced. Sequencing of 72 clones revealed 42 unique inserts containing microsatellites, out of which 19 primer pairs were designed and microsatellite amplification was tested on 39 wild hops and cultivars. Eleven primer pairs showed single locus amplification with 2,13 alleles, average 7.2, of which 17 unique alleles were discovered. One primer pair amplified too strong stutter bands, one locus was monomorphic and multilocus amplification was obtained with the remaining six primer pairs. [source] | ||||||||||