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Home Laboratory (home + laboratory)
Selected AbstractsA simple method for motorized alignment of Osmic confocal opticsJOURNAL OF APPLIED CRYSTALLOGRAPHY, Issue 6 2006John R. Ruble A system for the remote alignment of X-ray optics has been designed to address safety considerations related to manual optic adjustment. The described system also addresses some of the common problems associated with the alignment process, such as binding of components and the effects of external forces due to user contact that lead to irreproducibility of the process. The system as described should be applicable to most home laboratories. [source] Submicron resolution X-ray diffraction from periodically patterned GaAs nanorods grown onto Ge[111]PHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 8 2009Anton Davydok Abstract We present high-resolution X-ray diffraction pattern of periodic GaAs nanorods (NRs) ensembles and individual GaAs NRs grown catalyst-free throughout a pre-patterned amorphous SiNx mask onto Ge[111]B surfaces by selective-area MOVPE method. To the best of our knowledge this is the first report about nano-structure X-ray characterization growth on non-polar substrate. The experiment has been performed at home laboratory and using synchrotron radiation using a micro-sized beam prepared by compound refractive lenses. Due to the non-polar character of the substrate the shapes of NRs appear not uniform and vary between deformed hexagonal and trigonal in symmetry. Because the average diameter of NRs equals the experimental resolution certain cuts through slightly inclined edges or corners of individual NRs with lateral size of about 225,nm could be selected using spatially resolved reciprocal space mapping. [source] Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5ACTA CRYSTALLOGRAPHICA SECTION D, Issue 3 2004Tarja Parkkinen Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 86.3, b = 137.5, c = 196.1,Å, , = , = , = 90°. Assuming a molecular weight of 50.3,kDa, the VM values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8,Å resolution has been collected at a home laboratory and a data set to 2.2,Å resolution has been collected using synchrotron radiation. [source] Cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (BIV2-Helix) in the fusion core of bovine immunodeficiency-like virus (BIV) gp40ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 2 2005Xiaodong Zhao The fusion core of bovine immunodeficiency virus (BIV) gp40 is proposed to be involved in membrane fusion. However, no crystal structures are yet available. A predicted protein module BIV2-Helix of BIVgp40 has been expressed in Escherichia coli and purified by chromatography. Recombinant BIV2-Helix was crystallized using the hanging-drop vapour-diffusion technique at 291,K. The crystals were grown in MPD and belonged to the primitive rhombohedral space group R3, with unit-cell parameters a = 39.17, b = 39.17, c = 295.05,Å and two molecules per asymmetric unit. X-ray diffraction data were collected to 1.76,Å in the home laboratory from a single crystal. [source] | ||||||||||