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Selected AbstractsEcological aspects of seed desiccation sensitivityJOURNAL OF ECOLOGY, Issue 2 2003John C. Tweddle Summary 1The ability of seeds to survive desiccation is an important functional trait and is an integral part of plant regeneration ecology. Despite this, the topic has received relatively little attention from ecologists. In this study, we examine the relationships between seed desiccation tolerance and two important aspects of plant regeneration ecology: habitat and dormancy. This is done by comparative analysis of a data set of 886 tree and shrub species from 93 families. 2The proportion of species displaying desiccation sensitive seeds declines as the habitat becomes drier, and possibly also cooler, although the latter observation requires cautious interpretation. Desiccation sensitivity is most common in moist, relatively aseasonal vegetation zones, but is infrequent in, though not absent from arid and highly seasonal habitats. 3The highest frequency of desiccation sensitivity occurs in non-pioneer evergreen rain forest trees, although 48% of the species examined have desiccation tolerant seeds. In contrast, all pioneer taxa within the data set have drying tolerant seeds. 4Desiccation sensitivity is more frequent in seeds that are non-dormant on shedding (c. 31%), than dormant (c. 9%). Highest frequencies of drying tolerance occur in seeds with physical or combinational dormancy, at 99% and 100%, respectively. 5Although there is an association between non-dormancy and desiccation sensitivity in both tropical and temperate zones, the relationship does not appear to be causal. 6Working from the hypothesis that seed desiccation sensitivity represents a derived state in extant species, we use the results to investigate and discuss possible ecological trade-offs and associated fitness advantages. These may explain the hypothesized repeated loss of this trait. The frequent association between large seed size and desiccation sensitivity is also considered. [source] Enigmatic polychaete jaws from the Silurian of Gotland, SwedenLETHAIA, Issue 3 2000Mats Eriksson The jaws of the new polychaetaspid polychaete, Oenonites? honki, from the Silurian of Gotland, Sweden, differ from most Palaeozoic polychaete jaws. They exhibit enigmatic microstructural features in that they appear rough and give a corroded, or weathered impression. The altered microstructure of the jaws suggests a jaw chemistry and/or composition differing in some way from that of the co-occurring polychaete taxa. The jaws appear to have limited preservational potential and/or were particularly susceptible to secondary processes, resulting in microstructural alteration. Commonly, a row of distinct pits occurs on the outer face, especially of the first right maxillae (MIr). Because these pits are interpreted as associated with the dentary, the term ,denticle marks' is suggested. The pits may be the result of primary or secondary physical wear, or, more probably, secondary chemical alteration of localized mineral deposits. The primary function of such mineral deposits was to harden those parts of the surface that were exposed to great stress. The restricted occurrence of O.? honki, coupled with occasional increases in abundance (especially in the Halla Formation, unit b), indicates a preference for shallow marine, high-energy environments, particularly in reefal pockets with calcilutitic sediments. Highest frequency coincides with faunas characteristically containing a few labidognath species also displaying high frequencies. [source] Control of Environmental Lighting and Its Effects on Behaviors of the Alzheimer's TypeJOURNAL OF INTERIOR DESIGN, Issue 2 2002Melinda La Garce M.F.A. ABSTRACT The study investigates environmental lighting interventions designed to control the natural daylight effects of the setting sun and resultant behavior change. The purpose of this study was to determine if the frequency of disruptive behaviors of the Alzheimer's type that are defined across the literature to include wandering, anxiousness, combativeness, negative verbalizations, pilfering/hoarding, inappropriate sexual behavior, inappropriate emotional behavior, attention seeking, repetitive statements, and behaviors that are apparently precipitated and/or intensified by the effects of the setting sun i.e., changes in color, angles, and intensity of daylight, can be altered by environmental lighting interventions designed to control the daylight effects of the setting sun. This learning/practice partnership brought together the diverse expertise of research team members and provided new ways of examining research questions. Subjects were evaluated by medical practitioners to determine the probable presence of Alzheimer's disease. Disruptive behaviors were identified by trained observers reviewing 100 hours of videotaped observation, and videotaped observations of the subjects continued as subjects rotated monthly for four months between two apparently identical environments,one controlled and one experimental using environmental lighting interventions. Trained observers made double blind observation of subjects and recorded the frequency of disruptive behaviors on behavior observation checksheets. Tabulations of the disruptive behaviors were made, and percentage of change was calculated. A drop of 41% in the disruptive behaviors of subjects, while in the experimental environment, was demonstrated in the first rotation cycle, and an 11 % drop in disruptive behaviors was found in a second cycle. Inter-rater reliability across all tapes was 70%. Individuals exhibiting the highest frequencies of disruptive behaviors also demonstrated the most dramatic decreases in these behaviors while in the experimental environment. Environmental lighting interventions designed for this study appear to lessen the detrimental behavioral| effects of the setting sun on the behaviors of individuals with Alzheimer's disease. [source] Spectral index of the H2O-maser-emitting planetary nebula IRAS 17347 , 3139MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2005J. F. Gómez ABSTRACT We present radio-continuum observations of the planetary nebula (PN) IRAS 17347 , 3139 (one of the only two known to harbour water maser emission), made to derive its spectral index and the turnover frequency of the emission. The spectrum of the source rises in the whole frequency range sampled, from 2.4 to 24.9 GHz, although the spectral index seems to decrease at the highest frequencies (0.79 ± 0.04 between 4.3 and 8.9 GHz, and 0.64 ± 0.06 between 16.1 and 24.9 GHz). This suggests a turnover frequency of around 20 GHz (which is unusual among PNe, whose radio emission usually becomes optically thin at frequencies <10 GHz), and a relatively high emission measure (1.5 × 109 cm,6 pc). The radio-continuum emission has increased by a factor of ,1.26 at 8.4 GHz in 13 yr, which can be explained as expansion of the ionized region by a factor of ,1.12 in radius with a dynamical age of ,120 yr and at an expansion velocity of ,5,40 km s ,1. These radio-continuum characteristics, together with the presence of water maser emission and a strong optical extinction, suggest that IRAS 17347 , 3139 is one of the youngest PNe known, with a relatively massive progenitor star. [source] Genetic heritage and native identity of the Seaconke Wampanoag tribe of massachusettsAMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 4 2010Sergey I. Zhadanov Abstract The name "Wampanoag" means "Eastern People" or "People of the First Light" in the local dialect of the Algonquian language. Once extensively populating the coastal lands and neighboring islands of the eastern United States, the Wampanoag people now consist of two federally recognized tribes, the Aquinnah and Mashpee, the state-recognized Seaconke Wampanoag tribe, and a number of bands and clans in present-day southern Massachusetts. Because of repeated epidemics and conflicts with English colonists, including King Philip's War of 1675,76, and subsequent colonial laws forbidding tribal identification, the Wampanoag population was largely decimated, decreasing in size from as many as 12,000 individuals in the 16th century to less than 400, as recorded in 1677. To investigate the influence of the historical past on its biological ancestry and native cultural identity, we analyzed genetic variation in the Seaconke Wampanoag tribe. Our results indicate that the majority of their mtDNA haplotypes belongs to West Eurasian and African lineages, thus reflecting the extent of their contacts and interactions with people of European and African descent. On the paternal side, Y-chromosome analysis identified a range of Native American, West Eurasian, and African haplogroups in the population, and also surprisingly revealed the presence of a paternal lineage that appears at its highest frequencies in New Guinea and Melanesia. Comparison of the genetic data with genealogical and historical information allows us to reconstruct the tribal history of the Seaconke Wampanoag back to at least the early 18th century. Am J Phys Anthropol 142:579,589, 2010. © 2010 Wiley-Liss, Inc. [source] A genetic analysis of the Sakishima islanders reveals no relationship with Taiwan aborigines but shared ancestry with Ainu and main-island JapaneseAMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 2 2010Hirotaka Matsukusa Abstract The Sakishima islands are members of the Ryukyu island chain, stretching from the southwestern tip of the Japanese archipelago to Taiwan in the East China Sea. Archaeological data indicate cultural similarities between inhabitants of prehistoric Sakishima and Neolithic Taiwan. Recent studies based on tooth crown traits show remarkably high inter-island diversity among Ryukyu islanders, suggesting that the Sakishima islanders might have genetic backgrounds distinct from main-island Okinawa people. To investigate the genetic diversity of the Ryukyu islanders, we analyzed mtDNA, Y chromosome, and autosomal short tandem repeat loci in a sample of main-island Okinawa people and Sakishima (Miyako and Ishigaki) islanders whose participated in a previous study of tooth crown morphology. Our phylogenetic analysis of maternal (mtDNA) and paternal (Y chromosome) lineages shows that the Sakishima islanders are more closely related to people from the Japanese archipelago than to Taiwan aborigines. Miyako islanders and the Hokkaido Ainu have the first and second highest frequencies (respectively) of the Y-chromosomal Alu-insertion polymorphism, which is a presumable Jomon marker. Genetic diversity statistics show no evidence of demographic reduction or of extreme isolation in each island's population. Thus, we conclude that 1) Neolithic expansion from Taiwan did not contribute to the gene pool of modern Sakishima islanders, 2) male-lineage of the Ryukyu islanders likely shares a common ancestor with the Hokkaido Ainu who are presumably direct descendants of the Jomon people, and 3) frequent reciprocal gene flow among islands has masked the trace of common ancestry in the Ryukyu island chain. Am J Phys Anthropol, 2010. © 2010 Wiley-Liss, Inc. [source] Brief communication: Restricted geographic distribution for Y-Q* paragroup in South AmericaAMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 3 2009Graciela Bailliet Abstract We analyzed 21 paragroup Q* Y chromosomes from South American aboriginal and urban populations. Our aims were to evaluate the phylogenetic status, geographic distribution, and genetic diversity in these groups of chromosomes and compare the degree of genetic variation in relation to Q1a3a haplotypes. All Q* chromosomes from our series and five samples from North American Q* presented the derivate state for M346, that is present upstream to M3, and determined Q1a3* paragroup. We found a restrictive geographic distribution and low frequency of Q1a3* in South America. We assumed that this low frequency could be reflecting extreme drift effects. However, several estimates of gene diversity do not support the existence of a severe bottleneck. The mean haplotype diversity expected was similar to that for South American Q1a3* and Q1a3a (0.478 and 0.501, respectively). The analysis of previous reports from other research groups and this study shows the highest frequencies of Q* for the West Corner and the Grand Chaco regions of South America. At present, there is no information on whether the phylogenetic status of Q* paragoup described in previous reports is similar to that of Q1a3* paragroup though our results support this possibility. Am J Phys Anthropol, 2009. © 2009 Wiley-Liss, Inc. [source] Positive inotropic effect of coenzyme Q10, omega-3 fatty acids and propionyl-L-carnitine on papillary muscle force-frequency responses of BIO TO-2 cardiomyopathic Syrian hamstersBIOFACTORS, Issue 1-4 2008Romina Vargiu Abstract The inability of heart muscle to generate ventricular pressure to adequately propel blood through the cardiovascular system is a primary defect associated with congestive heart failure (CHF). Force-frequency relationship (FFR) is one of the main cardiac defects associated with congestive heart failure. Thus FFR is a convenient methodological tool for evaluating the severity of muscle contractile dysfunction and the effectiveness of therapeutic agents. Papillary muscle isolated from BIO TO-2 cardiomyopathic Syrian hamsters (CMSHs), show a depressed FFR and represents an animal model of human idiopathic dilated cardiomyopathy. In the present study we investigated the effect of CoQ10, omega-3 fatty acids, propionyl-L-carnitine (PLC) and a combination of these 3 agents (formulation HS12607) on FFR in 8 month old BIO TO-2 CMSHs. Papillary muscles isolated from the anesthetized animals were placed in an incubation bath and attached to an isometric force transducer. A digital computer with an analog/digital interface allowed control of both muscle developed force and electrical stimulus parameters. Force-frequency response was evaluated, at Lmax, with increasing frequencies: 0.06, 0.12, 0.25, 0.5, 1, 2 and 4 Hz. HS12607-treatment produced a positive inotropic effect resulting in a significant enhancement (p < 0.05) of the peak force at the highest frequencies (1,4 Hz). In the range of frequency of 1,4 Hz also CoQ10 and omega-3 significantly(p < 0.05) attenuated the fractional decline in developed force. The significant improvement (p < 0.05) of the timing parameter peak rate of tension rise (+T') and peak rate of tension fall (,T') indicating a faster rate of muscle contraction and relaxation respectively, found in CoQ10, omega-3 and PLC-treated CMSHs, may be due to the positive effects of these substances on sarcoplasmic reticulum functions. These findings suggest that naturally occurring CoQ10, omega-3 and PLC, particularly when administered together in a coformulation, might be a valid adjuvant to conventional therapy in dilated cardiomyopathy especially when considering that they are natural substances, devoid of side effects. [source] Cluster analysis of immunohistochemical markers in leiomyosarcoma delineates specific anatomic and gender subgroupsCANCER, Issue 18 2009Jason C. Carvalho MD Abstract BACKGROUND: Leiomyosarcoma (LMS) can be categorized into uterine, retroperitoneal, nonretroperitoneal soft tissue, cutaneous, visceral, and osseous anatomic subtypes. The differential expression of smooth muscle markers, estrogen receptor (ER), progesterone receptor (PR), and Wilms tumor-1 protein (WT1) by anatomic subtype and gender was explored. METHODS: A total of 78 LMS comprised of 30 uterine and 48 nonuterine tumors were studied. Nonuterine tumors were comprised of 17 soft tissue, 16 retroperitoneal, 7 cutaneous, 5 visceral, and 3 osseous subtypes. Immunohistochemical staining intensity on tissue microarray slides was scored as 0, 1+, or 2+, and cluster analysis was performed on the data. RESULTS: Smooth muscle actin was the most sensitive antibody (95%), followed by muscle-specific actin (91%), calponin (88%), desmin (73%), caldesmon (66%), and myosin (64%). Caldesmon and myosin were usually coexpressed, and were highest in retroperitoneal tumors (94%). There was no discernable correlation noted between histologic differentiation and smooth muscle marker expression. ER was much more common in women, with the highest frequencies noted in female retroperitoneal (86%) and uterine (63%) tumors. Nuclear WT1 was expressed in 11% of all tumors, and was limited to ER-positive uterine and female retroperitoneal tumors. Cluster analysis segregated 4 groups, most notably 1 driven by ER and PR, with the vast majority being uterine and female retroperitoneal tumors. CONCLUSIONS: Smooth muscle markers demonstrated variable sensitivities in LMS, with a tendency for anatomic subtypes to segregate based on expression patterns of these markers. ER defined a subgroup of uterine and female retroperitoneal tumors, and WT1 was limited to such tumors, suggesting a common line of differentiation as well as potential therapeutic targets. Cancer 2009. © 2009 American Cancer Society. [source] Soil erosion assessment using geomorphological remote sensing techniques: an example from southern ItalyEARTH SURFACE PROCESSES AND LANDFORMS, Issue 3 2010Sergio Lo Curzio Abstract The aim of this study is to assess of the distribution and map the geomorphological effects of soil erosion at the basin scale identifying newly-formed erosional landsurfaces (NeFELs), by means of an integration of Landsat ETM 7+ remotely sensed data and field-surveyed geomorphological data. The study was performed on a 228·6,km2 -wide area, located in southern Italy. The study area was first characterized from a lithological, pedological, land-use and morpho-topographic point of view and thematic maps were created. Then, the georeferenced Landsat ETM 7+ satellite imagery was processed using the RSI ENVI 4.0 software. The processing consisted of contrast stretching, principal component analysis (PCA), decorrelation stretching and RGB false colour compositing. A field survey was conducted to characterize the features detected on the imagery. Particular attention was given to the NeFELs, which were located using a global positioning system (GPS). We then delimited the Regions of Interest (ROI) on the Landsat ETM 7+ imagery, i.e. polygons representing the ,ground-truth', discriminating the NeFELs from the other features occurring in the imagery. A simple statistical analysis was conducted on the digital number (DN) values of the pixels enclosed in the ROI of the NeFELs, with the aim to determine the spectral response pattern of such landsurfaces. The NeFELs were then classified in the entire image using a maximum likelihood classification algorithm. The results of the classification process were checked in the field. Finally, a spatial analysis was performed by converting the detected landsurfaces into vectorial format and importing them into the ESRI ArcViewGIS 9.0 software. Application of these procedures, together with the results of the field survey, highlighted that some ,objects' in the classified imagery, even if displaying the same spectral response of NeFELs, were not landsurfaces subject to intense soil erosion, thus confirming the strategic importance of the field-checking for the automatically produced data. During the production of the map of the NeFELs, which is the final result of the study, these ,objects' were eliminated by means of simple, geomorphologically-coherent intersection procedures in a geographic information system (GIS) environment. The overall surface of the NeFELs had an area of 22·9,km2, which was 10% of the total. The spatial analysis showed that the highest frequency of the NeFELs occurred on both south-facing and southwest-facing slopes, cut on clayey-marly deposits, on which fine-textured and carbonate-rich Inceptisols were present and displaying slope angle values ranging from 12° to 20°. The comparison of two satellite imageries of different periods highlighted that the NeFELs were most clearly evident immediately after summer tillage operations and not so evident before them, suggesting that these practices could have played an important role in inducing the erosional processes. Copyright © 2009 John Wiley & Sons, Ltd. [source] Bauxite manufacturing residues from Gardanne (France) and Portovesme (Italy) exert different patterns of pollution and toxicity to sea urchin embryosENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 6 2002Giovanni Pagano Abstract This study was designed to investigate the composition and toxicity of solid residues from bauxite manufacturing plants. Soil and dust samples were collected in the proximity of two bauxite plants (Gardanne, France, and Portovesme, Italy). Samples were analyzed for their content of some selected inorganic contaminants by means of inductively coupled plasma optical emission spectroscopy (ICP-OES) either following acid digestion procedures or by seawater release of soluble components. Toxicity was tested by sea urchin bioassays to evaluate a set of toxicity endpoints including acute embryotoxicity, developmental defects, changes in sperm fertilization success, transmissible damage from sperm to the offspring, and cytogenetic abnormalities. Inorganic analysis showed two distinct sets of inorganic contaminants in Gardanne versus Portovesme, including Al, Cr, Cu, Fe, Mn, Pb, Ti, and Zn; sample composition (seawater-soluble cotaminants) and toxicity showed a noteworthy association. The most severe toxicity to embryogenesis and to sperm fertilization success was exerted by some Portovesme samples (0.03,0.5% w/v), with a significant association between toxicity and dose-related seawater release of Zn, Pb, and Mn. Seawater extraction of a toxic dust sample (G20) from the Gardanne factory showed increasing seawater release of Al, Fe, and Mn; the G20 sample, at the level of 0.5%, affected both developing sea urchin embryos and sperm (offspring quality). Soil samples around the Gardanne factory showed the highest frequency of toxic soil sites eastward from the factory. The present data point to solid deposition from bauxite plants as a potential subject of environmental health concern. The results suggest that extraction methods for evaluating the toxicity of complex mixtures should be based on the environmental availability of mixture components. The differences in sample toxicity among the tested sites, however, suggest possible site-to-site variability in geochemical and/or technological parameters. [source] Temporal dynamics of dissolved oxygen in a floating,leaved macrophyte bedFRESHWATER BIOLOGY, Issue 8 2008KARA GOODWIN Summary 1. Oxygen concentrations in shallow vegetated areas of aquatic systems can be extremely dynamic. In these waters, characterizing the average oxygen content or frequency of low oxygen events (hypoxia) may require high frequency measurements that span seasons and even years. In this study, moored sondes were used to collect 15-min interval dissolved oxygen (DO) readings in an embayment of the tidal Hudson River with dense coverage by an invasive floating leaved plant (Trapa natans) and in adjacent open waters. Measurements were made from late spring to summer over a 2-year period (2005, 2006). 2. Oxygen concentrations were far more dynamic in the vegetated embayment than in the adjacent open waters and while hypoxic conditions never occurred in the open waters, they occurred frequently in the vegetated site. Overall the vegetated site was hypoxic (DO < 2.5 mg L,1) 30% of the time and had an average oxygen concentration of 5.1 mg L,1. Oxygen concentration was significantly (P < 0.0001, anova) related to season, year and tide. Low tide periods during summer of 2006 had the lowest average oxygen concentration and the highest frequency of hypoxia. 3. The greater hypoxia in summer than spring is related to changes in plant morphology. In the spring and early summer when plants are submersed hypoxia occurs at lower frequency and duration than in the summer when dense floating vegetation covers the water. The tidal pattern in oxygen is related to hydrologic exchange with the non-vegetated open waters. Year-to-year variation may be related to relatively small changes in plant biomass between years. 4. Oxygen concentrations in aquatic systems can be critical to habitat quality and can have cascading impacts on redox sensitive nutrient and metal cycling. For some systems with dynamic oxygen patterns neither weekly spot sampling nor short-duration, high-frequency measurements may be sufficient to characterize oxygen conditions of the system. [source] ORIGINAL ARTICLE: Pro-hepcidin and iron metabolism parameters in multi-time blood donorsINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 5 2010J. BOINSKA Summary A high number of blood donations may cause iron depletion. The pathophysiology behind this process may involve hepcidin, a recently discovered peptide that acts by inhibiting iron absorption and promoting iron retention in reticuloendothelial macrophages. The aim of this study was to determine serum pro-hepcidin levels and iron metabolism parameters in multi-time blood donors. The study group consisted of 132 multi-time male blood donors and 25 healthy male volunteers (nondonors). Complete blood cell count and iron status including serum iron, ferritin, soluble transferrin receptor (sTfR), total iron binding capacity (TIBC), unsaturated iron binding capacity (UIBC), erythropoietin and pro-hepcidin (ELISA) were assessed. In blood donors, ferritin level drops markedly in relation to donation frequency (P < 0.001). In contrast, TIBC and UIBC levels increase progressively corresponding to annual donation frequency. Pro-hepcidin concentration increases significantly with the number of donations per year (P = 0.0290). In blood donors having donated blood with the highest frequency per year, pro-hepcidin levels were positively correlated with haemoglobin (R = 0.31, P < 0.05) and negatively with sTfR (R = ,0.31, P < 0.05). Pro-hepcidin levels increase in relation to blood donation frequency per year. Longitudinal studies focusing on changes in serum hepcidin levels are required to address the question whether hepcidin may contribute to iron metabolism disturbances in multi-times blood donors. [source] Glutamate dehydrogenase activity in lactobacilli and the use of glutamate dehydrogenase-producing adjunct Lactobacillus spp. cultures in the manufacture of cheddar cheeseJOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2006A.G. Williams Abstract Aims:, The study was undertaken to investigate the occurrence of glutamate dehydrogenase activity in different species of lactobacilli, and to determine, in a series of cheese-making trials, the effects of glutamate dehydrogenase-producing adjunct cultures on sensory attribute development during the maturation of cheddar cheese. Methods and Results:, The presence of dehydrogenase activity with glutamate as substrate was monitored in cell lysates of >100 strains from 30 different species of lactobacilli using a qualitative colorimetric plate screening assay. Activity was detectable in 25 of the 29 representative species obtained from culture collections and in 12 of the 13 non-starter species isolated from cheese. There were pronounced interspecies and strain differences in the occurrence, level and pyridine nucleotide specificity of the glutamate dehydrogenase activity detected. Among the non-starter lactobacilli the highest frequency of enzyme occurrence and activity was detected in the Lactobacillus plantarum isolates. The establishment of glutamate dehydrogenase-producing adjunct strains in the predominant population of lactobacilli in the cheese curd affected the formation of a number of volatile compounds in ripening cheddar cheese, while the presence of Lact. plantarum strains, in particular, was associated with an intensification and acceleration of aroma and flavour development during the maturation period. Conclusions:, Glutamate dehydrogenase formation by lactobacilli is a strain-dependent metabolic attribute, and adjunct cultures expressing the activity that are able to proliferate during cheese ripening have a positive impact on the rate of development and the intensity of cheddar cheese aroma and flavour development. Significance and Impact of the Study:, It has been demonstrated that some strains of glutamate dehydrogenase-producing lactobacilli have potential use as adjunct cultures to accelerate and intensify aroma and flavour formation during the manufacture of cheddar and, by analogy, other similar varieties of cheese. The importance of phenotypic discriminative monitoring of the dominant lactobacilli present during ripening to confirm adjunct establishment and population complexity was highlighted as was the requirement to establish the metabolic attributes of the non-starter population in uninoculated control cheeses in comparative trials. [source] Comparison of plain ice and flavoured ice for preventing oral mucositis associated with the use of 5 fluorouracilJOURNAL OF CLINICAL NURSING, Issue 6 2005Sue Nikoletti RN Aims and objectives., The study aimed to compare the use of plain ice, flavoured ice and standard care, to evaluate the effect on mucositis and to determine patients' perceptions of the two forms of oral cryotherapy. Background., Despite evidence that oral cryotherapy is useful in preventing mucositis in patients receiving 5-fluorouracil, concerns have been expressed about its clinical utility, due to potential side effects and negative perceptions. Design., A randomized, controlled, crossover trial was conducted in the outpatient chemotherapy department of an acute care teaching hospital in Perth, Western Australia. Patients were randomized to receive each of three interventions across three cycles of chemotherapy: standard care alone; standard care plus plain ice; and standard care plus flavoured ice. Methods., Oral mucositis was assessed by nurses prior to each of the three chemotherapy cycles and 15 days after each intervention. Two assessment tools were used, the Oral Assessment Guide, and the Western Consortium Cancer Nursing Research Scale. Participants completed a questionnaire to determine their comfort and satisfaction with oral cryotherapy, as well as factors affecting compliance. Results., Findings from 67 patients revealed that when participants used standard care alone, they were significantly more likely to experience symptoms of mucositis than when they used either plain or flavoured ice. Odds ratios were at least threefold higher for standard care alone, varying according to the instrument used. The two main concerns reported were the taste of flavoured ice and the time required to complete the cryotherapy interventions. Side effects such as nausea, sensitivity and headache were reported more frequently for flavoured ice (n = 11) compared with plain ice (n = 5) and standard care (n = 1). Conclusions., Both forms of oral cryotherapy were effective in reducing the severity of oral mucositis after chemotherapy and were more effective than standard care alone. Flavoured ice was associated with the highest frequency of side effects. Relevance to clinical practice., The benefits of cryotherapy appear to outweigh the problems in this sample of patients. The intervention should be tailored to individual patients, based on preferences for plain versus flavoured ice and small chips vs. larger blocks. Unsweetened frozen fruit juices should be evaluated. Time constraints could be addressed by providing transportable containers of ice. [source] Malaysian Indians are genetically similar to Caucasians: CYP2C9 polymorphismJOURNAL OF CLINICAL PHARMACY & THERAPEUTICS, Issue 2 2006Z. Zainuddin MSc Summary Background:,CYP2C9 is one of the major drug metabolizing enzymes for many drugs including warfarin, NSAIDs and losartan. It is polymorphic in many populations. Data on the distribution of CYP2C9 and the implication of CYP2C9 polymorphism in the Malaysian population is lacking. Our objectives were therefore to investigate the prevalence of CYP2C9 variants among unrelated healthy volunteers of Malays, Chinese and Indians in Malaysia. Method:, Deoxyribonucleic acid was extracted using standard lysis methods. Allele specific polymerase chain reaction was performed for determination of CYP2C9*1, *2, *3, *4 and *5 variants according to Z. Zainuddin, L.K. Teh, A.W.M. Suhaimi, M.Z. Salleh, R. Ismail (2003, Clinica Chimica Acta, 336, 97). Result:, The Chinese had the highest frequency of CYP2C9*1 (321/330, 97·27%), followed by the Malays and the Indians (402 of 420, 95·71% and 291 of 330, 88·18%, respectively). CYP2C9*2 was not found in the Chinese. CYP2C9*3 were detected in all the three races with the Indians having the highest frequency of CYP2C9*3 (9·7%). The Indians had a frequency of CYP2C9*2 and *3 similar to Tamilians and Caucasians. Two of the Indians had *2/*3 and one had *3/*3 genotypes and are likely to be slow metabolizers. No subject with CYP2C9*4 and *5 were detected in our populations. Conclusion:,CYP2C9*2 and *3 were identified in our population. Indians are similar to Caucasians in terms of CYP2C9 genotypes and thus may respond to CYP2C9 substrates differently when compared with the Malays and Chinese in Malaysia. [source] Ecological aspects of seed desiccation sensitivityJOURNAL OF ECOLOGY, Issue 2 2003John C. Tweddle Summary 1The ability of seeds to survive desiccation is an important functional trait and is an integral part of plant regeneration ecology. Despite this, the topic has received relatively little attention from ecologists. In this study, we examine the relationships between seed desiccation tolerance and two important aspects of plant regeneration ecology: habitat and dormancy. This is done by comparative analysis of a data set of 886 tree and shrub species from 93 families. 2The proportion of species displaying desiccation sensitive seeds declines as the habitat becomes drier, and possibly also cooler, although the latter observation requires cautious interpretation. Desiccation sensitivity is most common in moist, relatively aseasonal vegetation zones, but is infrequent in, though not absent from arid and highly seasonal habitats. 3The highest frequency of desiccation sensitivity occurs in non-pioneer evergreen rain forest trees, although 48% of the species examined have desiccation tolerant seeds. In contrast, all pioneer taxa within the data set have drying tolerant seeds. 4Desiccation sensitivity is more frequent in seeds that are non-dormant on shedding (c. 31%), than dormant (c. 9%). Highest frequencies of drying tolerance occur in seeds with physical or combinational dormancy, at 99% and 100%, respectively. 5Although there is an association between non-dormancy and desiccation sensitivity in both tropical and temperate zones, the relationship does not appear to be causal. 6Working from the hypothesis that seed desiccation sensitivity represents a derived state in extant species, we use the results to investigate and discuss possible ecological trade-offs and associated fitness advantages. These may explain the hypothesized repeated loss of this trait. The frequent association between large seed size and desiccation sensitivity is also considered. [source] The Factor Structure of Chinese Personality TermsJOURNAL OF PERSONALITY, Issue 2 2009Xinyue Zhou ABSTRACT From the Contemporary Chinese Dictionary, 3,159 personality descriptors were selected and then ranked by the frequency of use. Among those, the top 413 terms with the highest frequency were administered to two independent large samples in China for self-ratings and peer ratings to explore the emic Chinese personality structure as well as to test the universality of other models. One- and two-factor structures found in previous studies of other languages were well replicated. Previous structures with more than two factors were not well replicated, but six- and seven-factor models were at least as well supported as the Big Five. Emic analysis indicated that a seven-factor structure was the most informative structure relatively salient across subsamples of self-ratings and peer ratings, across original and ipsatized data, and across differences in variable selections. These factors can be called Extraversion, Conscientiousness/Diligence, Unselfishness, Negative Valence, Emotional Volatility, Intellect/Positive Valence, and Dependency/Fragility. [source] A method of identifying and locating sea-breeze fronts in north-eastern Brazil by remote sensingMETEOROLOGICAL APPLICATIONS, Issue 3 2006O. Planchon Abstract A method of identifying sea-breeze fronts by remote sensing (visible and infrared data from the GOES-8 satellite) was applied to north-eastern Brazil. The aim of the study was to estimate the frequency of observed sea-breeze fronts and the average distance they penetrate inland at 1800 UTC. During the dry season (between September and December) in 2000, the highest frequency of sea-breeze fronts was recorded on the northern fringe of the study area. The sea-breeze fronts penetrated furthest inland between September and November, with a maximum distance from the coast at Ceará of 100 km. Copyright © 2006 John Wiley & Sons, Ltd. [source] Types of article published in physiotherapy journals: a quantitative analysisPHYSIOTHERAPY RESEARCH INTERNATIONAL, Issue 4 2009Matteo Paci Abstract Background and Purpose.,The aim of this study was to quantify and assess the types of research and review articles published in physiotherapy professional journals with international circulations.,Method.,Selected articles from nine physiotherapy journals in a period of five years were classified according to their study design.,Results.,A total number of 1627 articles were included in this study of which 205 (12.60%) were randomized controlled trial (RCT). Articles published with the highest frequency were ,observational/descriptive studies' (n = 305, 18.75%), whereas ,meta-analyses' were the rarest ones (n = 21, 1.29%). Many articles were validation studies (n = 216, 13.28%) and articles classified as ,others' (n = 217, 13.34%). High heterogeneity was found in terms of distribution over years and journals with an improvement of the number of RCTs among years.,Conclusion.,The distribution of different types of article in physiotherapy journals is similar to other disciplines. The improvement of RCTs represents an important move in the right direction for evidence-based physiotherapy research. Copyright © 2009 John Wiley & Sons, Ltd. [source] Changes in the highest frequency of breath sounds without wheezing during methacholine inhalation challenge in childrenRESPIROLOGY, Issue 3 2010Chizu HABUKAWA ABSTRACT A breath sound analyser was used to detect bronchoconstriction without wheezing during methacholine inhalation challenge in children. The highest frequency of inspiratory breath sounds increased significantly during bronchoconstriction and decreased after inhalation of a bronchodilator. The highest frequency of inspiratory breaths sounds was correlated with bronchial reactivity. Background and objective: It is difficult for clinicians to identify changes in breath sounds caused by bronchoconstriction when wheezing is not audible. A breath sound analyser can identify changes in the frequency of breath sounds caused by bronchoconstriction. The present study aimed to identify the changes in the frequency of breath sounds during bronchoconstriction and bronchodilatation using a breath sound analyser. Methods: Thirty-six children (8.2 ± 3.7 years; males : females, 22 : 14) underwent spirometry, methacholine inhalation challenge and breath sound analysis. Methacholine inhalation challenge was performed and baseline respiratory resistance, minimum dose of methacholine (bronchial sensitivity) and speed of bronchoconstriction in response to methacholine (Sm: bronchial reactivity) were calculated. The highest frequency of inspiratory breath sounds (HFI), the highest frequency of expiratory breath sounds (HFE) and the percentage change in HFI and HFE were determined. The HFI and HFE were compared before methacholine inhalation (pre-HFI and pre-HFE), when respiratory resistance reached double the baseline value (max HFI and max HFE), and after bronchodilator inhalation (post-HFI and post-HFE). Results: Breath sounds increased during methacholine-induced bronchoconstriction. Max HFI was significantly greater than pre-HFI (P < 0.001), and decreased to the basal level after bronchodilator inhalation. Post-HFI was significantly lower than max HFI (P < 0.001). HFI and HFE were also significantly changed (P < 0.001). The percentage change in HFI showed a significant correlation with the speed of bronchoconstriction in response to methacholine (P = 0.007). Conclusions: Methacholine-induced bronchoconstriction significantly increased HFI, and the increase in HFI was correlated with bronchial reactivity. [source] Key tree species for the golden-headed lion tamarin and implications for shade-cocoa management in southern Bahia, BrazilANIMAL CONSERVATION, Issue 1 2010L. C. Oliveira Abstract The golden-headed lion tamarin Leontopithecus chrysomelas occurs in the Atlantic forest of southern Bahia, Brazil, where shade-cocoa agroforestry (known as cabruca) predominates. The economic decline of the cocoa industry has caused many landowners to convert cabruca into cattle pasture or diversify their plantations with other crops. These and prior anthropogenic disturbances such as habitat fragmentation are threatening lion tamarin persistence. For some lion tamarin groups, cabruca comprises a large part of their home range. Considering these factors, the maintenance of the biological diversity in cabruca favorable to golden-headed lion tamarins is of considerable interest to their long-term survival. Here we identify plant species that provide food and sleeping sites for the lion tamarins and examine their occurrence in cabruca plantations, in order to investigate alternatives for conservation management practices that benefit both lion tamarins and cabruca. We determined the total number of trees and the frequency of individuals and species used for food and sleeping sites by lion tamarins in Una Biological Reserve, Bahia, from 1998 to 2006. We used this information to compare the richness and frequency of use across habitats (cabruca, mature and secondary) and to create a ranking index considering various components of a tree species' utility to the lion tamarins. Lion tamarins used 155 tree species, 93 for food and 93 for sleeping sites. Fifty-five species were ranked as ,Extremely Valuable,' eight as ,Valuable' and 92 as ,Of Interest.' Of 48 families, Myrtaceae and Sapotaceae were used the most. Cabruca contained fewer individual trees used by lion tamarins, but the highest frequency of use per tree compared with other habitats, indicating the large influence of single trees in these plantations. Using the key tree species identified in our study in the management of cabruca would be of considerable benefit to the long-term survival of lion tamarins [source] Refined Geographic Distribution of the Oriental ALDH2*504Lys (nee 487Lys) VariantANNALS OF HUMAN GENETICS, Issue 3 2009Hui Li Summary Mitochondrial aldehyde dehydrogenase (ALDH2) is one of the most important enzymes in human alcohol metabolism. The oriental ALDH2*504Lys variant functions as a dominant negative, greatly reducing activity in heterozygotes and abolishing activity in homozygotes. This allele is associated with serious disorders such as alcohol liver disease, late onset Alzheimer disease, colorectal cancer, and esophageal cancer, and is best known for protection against alcoholism. Many hundreds of papers in various languages have been published on this variant, providing allele frequency data for many different populations. To develop a highly refined global geographic distribution of ALDH2*504Lys, we have collected new data on 4,091 individuals from 86 population samples and assembled published data on a total of 80,691 individuals from 366 population samples. The allele is essentially absent in all parts of the world except East Asia. The ALDH2*504Lys allele has its highest frequency in Southeast China, and occurs in most areas of China, Japan, Korea, Mongolia, and Indochina with frequencies gradually declining radially from Southeast China. As the indigenous populations in South China have much lower frequencies than the southern Han migrants from Central China, we conclude that ALDH2*504Lys was carried by Han Chinese as they spread throughout East Asia. Esophageal cancer, with its highest incidence in East Asia, may be associated with ALDH2*504Lys because of a toxic effect of increased acetaldehyde in the tissue where ingested ethanol has its highest concentration. While the distributions of esophageal cancer and ALDH2*504Lys do not precisely correlate, that does not disprove the hypothesis. In general the study of fine scale geographic distributions of ALDH2*504Lys and diseases may help in understanding the multiple relationships among genes, diseases, environments, and cultures. [source] Age of SERPINA1 Gene PI Z Mutation: Swedish and Latvian Population AnalysisANNALS OF HUMAN GENETICS, Issue 3 2008B. Lace Summary Alpha 1-antitrypsin (A1AT) deficiency, one of the most common inborn errors of metabolism in Caucasians, is characterized by a low serum concentration of A1AT and a high risk of pulmonary emphysema and liver disease. The allelic frequency for the most common protease inhibitor (PI) Z mutation in the SERPINA1 gene is 2,5% in Caucasians of European descent. The objective of our study was to estimate the PI Z mutation age using molecular analysis in Latvian and Swedish populations, which have the highest frequency of PI Z mutation. DNA samples of heterozygous and homozygous PI Z allele carriers from Latvia (n = 21) and Sweden (n = 65) were analysed; 113 unrelated healthy donors from Latvia were used as a control group. MALDI-TOF analysis was performed on all samples. Pairwise Fst was computed to compare the PI Z mutation ages between the two populations and controls. A p value less than 0.05 was considered significant. Analysis of non-recombinant SNPs revealed that the PI Z mutation age was 2902 years in Latvia (SD 1983) and 2362 years in Sweden (SD 1614) which correlates with previous studies based on microsatellite analysis. [source] Polymorphic Alu Insertions and their Associations with MHC Class I Alleles and Haplotypes in the Northeastern ThaisANNALS OF HUMAN GENETICS, Issue 4 2005D. S. Dunn Summary Polymorphic Alu insertions (POALINs) are known to contribute to the strong polymorphic nature of the Major Histocompatibility Complex (MHC). Previous population studies on MHC POALINs were limited to only Australian Caucasians and Japanese. Here, we report on the individual insertion frequency of the five POALINs within the MHC class I region, their HLA-A and -B associations, and the three and four locus alpha block POALIN haplotype frequencies in the Northeastern (NE) Thai population. Of the five POALINs, the lowest frequency was 0.018 for AluyHF and the highest frequency was 0.292 for AluyHJ and AluyHG. The strongest positive associations between the POALINs and HLA class I alleles was between AluyMICB and HLA-B*57, AluyHJ and HLA-A*24 and HLA-A*01, and AluyHG and HLA-A*02, supporting previous findings in Caucasians and Japanese. Single POALIN haplotypes were found more frequently than multiple POALIN haplotypes. However, of the seven different POALIN haplotypes within the MHC alpha block, there were only two significant differences between the NE Thais, Caucasians and Japanese. This study confirms that the MHC POALINs are in linkage disequilibrium with HLA-A and ,B alleles and that there are significant frequency differences for some of the POALINs when compared between NE Thai, Caucasians and Japanese. [source] Psychiatric disturbances in CADASIL: a brief reviewACTA NEUROLOGICA SCANDINAVICA, Issue 5 2008R. Valenti Background,,, Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is an inherited cerebrovascular disease, clinically characterized by a variable combination of migraine, recurrent transient ischemic attack (TIA) or lacunar strokes, cognitive decline, and mood disturbances. However, the assessment of psychiatric disturbances in this disease has never been carried out systematically. Methods,,, This paper presents a brief review of the literature regarding the occurrence of psychiatric disorders in CADASIL patients. Results,,, The prevalence of psychiatric disorders in CADASIL patients is reported to range from 20% to 41%. The psychiatric disturbances reported with the highest frequency are mood disturbances (9,41%). Pooling together the studies and considering a total of 454 CADASIL patients reported in the literature, 106 of these were affected by mood disturbances (24%). The majority of studies however did not use any defined criteria to assess the presence of psychiatric disorders and diagnoses were mainly based on history or review of clinical records. Conclusions,,, The review of CADASIL literature suggests the need for a more structured approach to the investigation of these disturbances that are highly prevalent and may greatly impact quality of life in these patients. [source] The impact of cytomorphology, cytogenetics, molecular genetics, and immunophenotyping in a comprehensive diagnostic workup of myelodysplastic syndromesCANCER, Issue 19 2009Ulrike Bacher MD Abstract BACKGROUND: Because of limited reproducibility of morphologic features, the morphological categorization of initial myelodysplastic syndromes (MDS) cases remains a major task in a diagnostic setting. METHODS: To further evaluate the role of additional diagnostic methods for suspected early MDS, the authors analyzed 1965 cases with unclear cytopenia where at least cytomorphology and immunophenotyping were performed in parallel, combined with cytogenetics and molecular genetics. RESULTS: In 353 patients, both methods diagnosed malignant/nonmalignant disease other than MDS, and 557 patients had MDS-refractory anemia with excess of blasts/chronic myelomonocytic leukemia. The remaining 1055 patients (53.7%), where early MDS/reactive cytopenia had to be assumed, were categorized into 6 groups depending on cytomorphology/immunophenotyping results for or against MDS. In 659 of 1055 cases (62.4%) with suspected initial MDS, cytomorphology and immunophenotyping were concordant in the categorization of MDS/non-MDS. Cytogenetics, available in 951 of 1055 patients, revealed the highest frequency of aberrant karyotypes when both cytomorphology and immunophenotyping proposed MDS (63 of 227; 27.8%). But also in the groups where either cytomorphology or immunophenotyping showed evidence of MDS, aberrant karyotypes were found in 6% to 14% of patients. Even when both morphology and immunophenotyping showed no MDS, 11 of 208 (5.3%) had cytogenetic aberrations. RUNX1/AML1 mutation screening was positive in 15% in the latter group. NRAS, MLL -PTD, NPM1, and JAK2V617F were detected in low frequencies, confirming MDS diagnosis in the respective cases. CONCLUSIONS: This report outlines the power of a combined diagnostic approach for suspected initial cases of MDS including immunophenotyping, cytogenetics, and molecular genetics with selected markers in addition to cytomorphology. Diagnostic algorithms should be developed, and immunophenotyping should be further validated for this specific indication. Cancer 2009. © 2009 American Cancer Society. [source] High-dose exposure to cat is associated with clinical tolerance , a modified Th2 immune response?CLINICAL & EXPERIMENTAL ALLERGY, Issue 12 2003B. Hesselmar Summary Background Allergen-specific IgG4 antibodies, it is suggested, may be protecting against allergy development by blocking responses. Levels are proposed as a marker of modified Th2 response. Objectives To assess the levels of IgE, IgG1 and IgG4 antibodies to cat in relation to cat exposure, asthma and allergic diseases. Methods We studied a population-based sample of 412 schoolchildren of 12,13 years of age. Parents of 402 children completed a questionnaire covering their child's medical history, the keeping of cats and other background data. Skin prick tests (SPTs) to common aeroallergens were performed in 371 of the children. Blood samples for the analyses of IgE, IgG1 and IgG4 antibodies were obtained from 309 of them. Results All children had an immune response to cat, predominantly of the IgG1 subclass. The levels of cat-specific IgG1 and IgG4, but not IgE, were high in children currently keeping a cat. Children with asthma had increased levels of cat-specific IgE and IgG1, and children with a positive SPT to cat also had increased IgG4. The presence of IgG4 was not associated with asthma or sensitization, unless there was a simultaneous production of IgE. Twenty-five percent of the children had an immune response with only IgG4, and no IgE antibodies to cat. This group of children had the highest frequency of cat-keeping, but a similar prevalence of asthma and allergy as those with neither IgE nor IgG4 antibodies to cat. Conclusion Cat-keeping was associated with a modified Th2 response, producing IgG4 but not IgE antibodies. This immune response was not associated with an increased risk of asthma or allergy. However, the IgG4 antibodies did not directly mediate any protective effect. 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