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High Protein (high + protein)
Terms modified by High Protein Selected AbstractsPrey protein influences growth and decoration building in the orb web spider Argiope keyserlingiECOLOGICAL ENTOMOLOGY, Issue 5 2009SEAN J. BLAMIRES Abstract. 1. Protein is important for a foraging animal to consume, as it promotes growth and enhances survival, particularly in web-building spiders, which need to invest considerable protein into web building and may trade-off growth for web investment. 2. The influence of dietary protein uptake on growth and web investment was tested in the orb web spider Argiope keyserlingi, by feeding them flies reared on three different media: (1) high protein, (2) low protein, and (3) standard (control) media. There was a negative correlation between protein and energy content of the flies across treatments; flies reared on the high protein media had the highest protein, but lowest energy, while flies reared on the low protein media had the lowest protein but highest energy. 3. It was found that silk investment and web architecture in A. keyserlingi was not affected by diet. Growth and decoration building were both enhanced when spiders were fed a high protein diet. 4. It was concluded that protein intake, rather than energy, influenced both growth and decoration building because: (1) protein intake enhances growth in other animals, (2) protein is essential for silk synthesis, especially aciniform silk, and (3) protein is a limiting factor actively sought by foraging animals in natural environments. [source] Effect of protein : energy ratio in milk replacers on growth performance of goat kidsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 5-6 2002K-H. YEOM Two separate experiments were carried out to establish the effects of the protein : energy ratio in milk replacers on growth performance, plasma lipid concentrations and fatty acid composition in adipose tissue of male goat kids. In the first experiment there were 211 3-day- old goat kids and in the second experiment there were 121 kids aged 3,7 days. The animals were fed ad libitum for a period of 4 weeks on milk replacers containing either 11.5 or 9.5 g crude protein/MJ metabolizable energy. In essence, protein was exchanged with fat on a weight basis. Milk concentrations were increased from 160 to 190 g/l in experiment 1, from 150 to 180 g/l in experiment 2. There were significant increases in body weight and feed intake when the milk replacer with high protein : energy ratio was fed. Group mean average daily weight gain was 168 and 203 g for the groups with low and high dietary protein : energy ratio in experiment 1; for experiment 2 the values were 139 and 160 g. Average dry matter intake was 18 and 14% higher for the diet with high protein : energy ratio in experiments 1 and 2, respectively. There was no change in either feed conversion (feed : gain ratio) or energy conversion (weight gain : energy intake ratio). There were no consistent diet effects on plasma lipid concentrations. Dietary fatty acid composition was reflected by that of adipose tissue. The milk replacer with high protein : energy ratio produced a small increase in the contents of myristic and palmitic acid in adipose tissue. [source] Effect of feeding complete feed block containing Prosopis cineraria leaves and polyethylene glycol (PEG)-6000 on nutrient intake, its utilization, rumen fermentation pattern and rumen enzyme profile in kidsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 11 2005Raghavendra Bhatta Abstract A study was carried out to determine the effect of replacing the cake portion of concentrate mixture with 5 parts polyethylene glycol (PEG)-6000 in a complete feed block (CFB) containing Prosopis cineraria leaves on the performance, rumen fermentation pattern and rumen enzyme profile of kids under an intensive system of rearing. Eighteen weaners of Sirohi goat of similar body weight (16.0 ± 0.5 kg) and age (90 ± 5 days) were divided into three equal groups. They were housed in individual cages in a side open asbestos roof shed with mud floor. All the kids received CFBs containing 50 parts P cineraria leaves and 50 parts concentrate mixture. CFB offered to the first group had high protein (183.8 g kg,1; HP), that offered to the second group had low protein (131.3 g kg,1; LP) and that offered to the third group had low protein (124.7 g kg,1) but contained PEG-6000 (LP-PEG). The concentrate mixtures in LP and LP-PEG were without groundnut cake, whereas in LP-PEG, groundnut cake was replaced by barley and 5 parts PEG-6000 were incorporated. CFBs were similar in their nutritive value except for crude protein (CP). Prosopis leaves utilized in the CFB contained (g kg,1 dry matter) 129.1 CP, 535.5 neutral detergent fibre, 395.8 acid detergent fibre and 222.8 acid detergent lignin. There were significant differences in dry matter intake (g day,1) between HP (1102), LP (1108) and LP-PEG (1194); the trend in Metabolizable energy intake was similar. During the growth trial, LP kids consumed maximum amount of feed (76.91 kg) followed by HP (75.73 kg) and LP-PEG (73.12 kg). However, maximum feed efficiency (feed consumed kg,1 live weight gain) was recorded in LP-PEG kids (9.59) followed by HP (10.64) and LP (11.60). These differences were statistically significant (p < 0.05). Although there was no significant difference in the digestibility of dry matter among the groups, there was significant difference in the digestibility of CP, neutral detergent fibre and acid detergent fibre. The digestibility of CP was 0.591, 0.484 and 0.645, that of neutral detergent fibre was 0.397, 0.308 and 0.499 and that of acid detergent fibre was 0.168, 0.154 and 0.282 in HP, LP and LP-PEG, respectively. Rumen metabolites studied 6 h after feeding revealed that there were significant (p < 0.05) differences in the concentrations of ammonia N, tri-chloro acetic acid precipitable N and total volatile fatty acids among the three groups, but not pH. The rumen enzyme concentrations showed significant (p < 0.05) differences for ,-amylase, whereas the differences were non-significant for CMcase and protease. After 90 days of feeding trial, the maximum weight gain was recorded in LP-PEG (7.62 kg) followed by HP (7.23 kg) and LP (6.53 kg). It can be concluded that, when kids are reared under an intensive system on complete feed blocks containing Prosopis leaves, high protein concentrate can be replaced with a low protein concentrate containing 5 parts PEG, which would not only alleviate the negative effects of tannin but would also spare expensive groundnut protein. Copyright © 2005 Society of Chemical Industry [source] Proximate composition, fatty acid analysis and protein digestibility-corrected amino acid score of three Mediterranean cephalopodsMOLECULAR NUTRITION & FOOD RESEARCH (FORMERLY NAHRUNG/FOOD), Issue 10 2006Spiros Zlatanos Abstract Proximate composition, fatty acid analysis and protein digestibility-corrected amino acid score (PDCAAS) in three commercially important cephalopods of the Mediterranean sea (cuttlefish, octopus and squid) were determined. The results of the proximate analysis showed that these species had very high protein:fat ratios similar to lean beef. Docosahexaenoic, palmitic and eicosipentaenoic acid were the most abundant fatty acids among analyzed species. The amount of n- 3 fatty acids was higher than that of saturated, monounsaturated and n- 6 fatty acids. Despite the fact that cephalopods contain small amounts of fat they were found quite rich in n- 3 fatty acids. Finally, PDCAAS indicated that these organisms had a very good protein quality. [source] Nutritional evaluation of some Nigerian wild seedsMOLECULAR NUTRITION & FOOD RESEARCH (FORMERLY NAHRUNG/FOOD), Issue 2 2004Ganiyu Oboh Abstract Some wild seeds, namely Parkia biglobosa, Tetracarpidum conophorum, Pentaclethra macrophylla, Irvingia gabonensis, Afzelia africana, Prosporis africana and Monodora myristica, were randomly collected from various parts of Nigeria and analyzed with regard to their proximate, mineral, antinutrient composition and zinc bioavailability. The results revealed that the seeds had high protein (6.5,24.2%), fat (19.0,58.5%), mineral (Mg, Fe, Zn, Mn, Ca, Na, K, P) and phytate (1043.6,2905.2 mg/100 g) contents, while the cyanide content was low (3.7,6.4 mg/kg). However, Co, Pb and Ni were not detected in all the samples. The calculated [Ca] [phytate]/[Zn] molar ratios (which is the best index for predicting Zn bioavailability) for all the seeds revealed that Parkia biglobosa, Irvingia gabonensis and Prosporis africana had a calculated molar ratio above 0.50 mol/kg (critical level), thus indicating reduced bioavailability of Zn to a critical level. In view of the high fat, protein, mineral and low cyanide contents, the high phytate content would not be expected to reduce bioavailability of Zn in some of the wild seeds (Afzelia africana, Pentaclethra macrophylla and Monodora myristica). These wild seeds could be good nutrient sources if integrated fully into human and animal nutrition. However, further studies will be carried out on the protein quality and toxicological potentials of these wild seeds. [source] Effects of dietary protein and lipid levels on growth, feed conversion and body composition in rohu, Labeo rohita (Hamilton), fingerlingsAQUACULTURE NUTRITION, Issue 1 2003B.B. Satpathy Abstract Twelve experimental diets (D-1 to D-12) in a 4 × 3 factorial design (four protein levels: 250, 350, 400 and 450 g kg,1 and three lipid levels: 50, 100 and 150 g kg,1) were formulated. Carbohydrate level was constant at 250 g kg,1. Rohu fingerlings (average wt. 4.3 ± 0.02 g) were fed the experimental diets for 60 days in three replicates at 2% BW day,1. Weight gain (%), specific growth rate (% day,1) and feed gain ratio (FGR) indicated that diets containing 450 g kg,1 protein and 100 or 150 g kg,1 lipid (diets D-11 and D-12) resulted in best performance, although results were not significantly different from those of diet D-9 (400 g kg,1 protein and 150 g kg,1 lipid). Protein efficiency ratio was highest with diets D-6 (350 g kg,1 protein and 150 g kg,1 lipid) and D-9 (400 g kg,1 protein and 150 g kg,1 lipid) (P > 0.05) and declined with higher and lower protein diets at all levels of lipid tested. Elevated lipid level (50, 100 or 150 g kg,1) did not produce better FGR in diets containing 400 and 450 g kg,1 dietary protein (P > 0.05). The combined effects of protein and lipid were evident up to 400 g kg,1 protein. Growth and FGR showed consistent improvement with increased lipid levels from 50 to 150 g kg,1 at each protein level tested except with diets containing 450 g kg,1 protein. Apparent nutrient digestibility (for protein, lipid and energy) did not show significant variation among different dietary groups (P > 0.05). Whole body protein and lipid contents increased significantly (P > 0.05) with dietary protein level. The results of this study indicate that rohu fingerlings are adapted to utilize high protein in diets with varying efficiency. The fish could utilize lipid to spare protein but there is no significant advantage from this beyond the dietary protein level of 350,400 g kg,1 in terms of growth and body composition. [source] Effects of dietary protein and lipid levels on growth performance and body composition of black sea bass Centropristis striata (Linnaeus 1758) during grow-out in a pilot-scale marine recirculating systemAQUACULTURE RESEARCH, Issue 4 2009Md Shah Alam Abstract The influence of four formulated practical diets, with different protein and lipid levels, on the growth and body composition of black sea bass (Centropristis striata L.) pre-adults was evaluated in a pilot-scale marine recirculating system. Four test diets were prepared with a combination of two protein levels (44% and 54%) and two lipid levels (10% and 15%). The diets were as follows: low protein and low lipid (LP:LL; 44:10), low protein and high lipid (LP:HL; 44:15), high protein and low lipid (HP:LL; 54:10) and high protein and high lipid (HP:HL; 54:15). Fish (mean weight=75.5 g) were fed the respective diets for 90 days. For fish fed LP:HL, body weight gain was significantly (P<0.05) higher than fish fed LP:LL. Increasing the protein level from 44% to 54% did not produce a significant effect on weight gain at high lipid level. A significant (P<0.01) interactive effect between dietary protein and lipid levels on the growth and feed utilization was observed. Total lipid content in the whole body, muscle and liver was significantly affected by the dietary lipid levels. The results suggested that a combination of 44% dietary protein and 15% lipid was optimal for the growth of black sea bass. [source] Effect of mixed feeding schedules with varying dietary protein levels on the growth of sutchi catfish, Pangasius hypophthalmus (Sauvage) with silver carp, Hypophthalmichthys molitrix (Valenciennes) in pondsAQUACULTURE RESEARCH, Issue 7 2005Md Zulfikar Ali Abstract A 6-month feeding trial was conducted in field condition using 10 farm ponds (400,600 m2) to investigate the effect of mixed feeding schedules on the growth of sutchi catfish, Pangasius hypophthalmus with silver carp, Hypophthalmichthys molitrix. Fish were stocked at a ratio of 80:20 (sutchi catfish, 4.9±0.5 g: silver carp, 12.0±0.8 g) at the total rate of 25 000 ha,1. Two diets of high protein (30%, HP) and low protein (16%, LP) were prepared using locally available feed ingredients. Five different feeding schedules of high-protein diet continuously (HP), low-protein diet continuously (LP), 1-day low,protein/1-day high-protein diet (1LP/1HP), 7 days low,protein/7 days high-protein diet (7LP/7HP) and 14 days low,protein/14 days high-protein diet (14LP/14HP) were tested. The fish were fed twice daily at the rate of 15%, 10%, 8% and 5% of their body weight for first, second, third month and rest of experimental period respectively. Feeding rate was calculated only on the basis of sutchi catfish weight only and was adjusted every 2 weeks according to weight gain. Fish fed LP and HP on alternate day (1LP/1HP) resulted in significantly (P<0.05) higher growth rate, feed utilization and production among the treatments. However, there were no significant differences (P>0.05) between the growth rates and production of fish fed HP regularly and fish fed 7 days LP followed by 7 days HP (7LP/7HP). Fish maintained on LP grew the least. The feed conversion ratio (FCR) values for sutchi catfish ranged between 2.04 and 2.79 with feeding schedule 1LP/1HP showing the best FCR. The total production of fish (including silver carp) ranged between 8310 and 12 422 kg ha,1 6 months,1 with 1LP/1HP feeding schedule resulting in the highest production and net profit. The study demonstrated that feeding fish continuously with HP is less economical. Thus, for profitable sutchi catfish culture with silver carp, farmers can use the mixed feeding schedule of alternate day feeding of LP and HP as a means of reducing feed costs. [source] Affinity Ligand Selection from a Library of Small Molecules: Assay Development, Screening, and ApplicationBIOTECHNOLOGY PROGRESS, Issue 1 2005Lakshmi D. Saraswat A facile and cost-effective process for screening synthetic libraries for an affinity ligand is described. A high throughput 96-well plate filtration method was designed to screen both discrete compounds and mixtures of compounds attached to a solid support. Human serum albumin (HSA) was used as a target protein to demonstrate the proof of concept. Detection and quantitation by fluorescence was accomplished with the use of fluorescamine to conjugate the protein in the filtrate. It is found that mixtures demonstrating low average binding reflect an overall lower hit rate of the components, whereas deconvolution of mixtures with high protein binding consistently provides a high hit rate. This differs from many of the previous experiences screening solid-phase mixtures in which high false positive rates are noted to occur. A total of 100K compounds were tested: 25K as discrete samples and 75K as mixtures. An overall hit rate of 8% was observed. Secondary screening of compounds measured specificity, recovery, and dynamic binding capacity. The effectiveness of the method is illustrated using an affinity column made with a representative lead compound. A similar purity was achieved in a single-step purification of HSA from serum as compared to that obtained by two steps of ion-exchange chromatography. The process for primary screening of a large number of compounds is simple, inexpensive, and applicable to any soluble target protein of known or unknown function from crude mixtures and may have additional utility as a generic chemical affinity tool for the functional characterization of novel proteins emerging from proteomics work. [source] | |||||||||||||