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High L (high + l)
Selected AbstractsChanges in amino acid composition in the tissues of African catfish (Clarias gariepinus) as a consequence of dietary L-carnitine supplementsJOURNAL OF APPLIED ICHTHYOLOGY, Issue 3 2002R. O. A. Ozório A study was undertaken to examine the effect of different amounts of dietary lysine (13 and 21 g kg,1 diet), lipid (80 and 160 g kg,1 diet) and L -carnitine (0.2 and 1.0 g kg,1 diet) on growth performance, proximate composition and amino acid metabolism of the African catfish (Clarias gariepinus). Juvenile African catfish (23 ± 1.5 g/fish) were stocked into 70-L aquaria (16 aquaria, 28 fish/aquarium) connected to a recirculation system during a maximum period of 74 days. All groups were fed at a level of 24 g kg,0.8 day,1 in an experiment run at pair feeding. Animals receiving 1.0 g carnitine accumulated up to six times more carnitine in their tissues than animals receiving 0.2 g (P < 0.05). Acyl-carnitine and free L -carnitine levels increased in the whole body and in tissues. Dietary L -carnitine supplements increased protein-to-fat ratios in the body, but did not affect growth rate. Protein-to-fat ratios were only affected when the biosynthesis capacity of L -carnitine was restricted due to low lysine levels and when there was a shortage of dietary fat. When lysine was offered at 21 g kg,1 feed, dietary L -carnitine supplements did not affect the amino acid concentrations of body tissues. Dietary L -carnitine supplements raised the concentration of glutamic acid,>,aspartic acid,>,glycine > alanine > arginine > serine > threonine in skeletal muscle tissue (P < 0.05). Total amino acid concentration in muscle and liver tissues (dry-matter basis) increased from 506 to 564 and from 138 to 166 mg g,1, respectively, when diets were offered with high L -carnitine, low lysine and low fat levels. These data suggest that dietary L -carnitine supplementation may increase fatty acid oxidation and possibly decrease amino acid combustion for energy. [source] What controls the C iv line profile in active galactic nuclei?MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2005Alexei Baskin ABSTRACT The high-ionization lines in active galactic nuclei (AGN), such as C iv, tend to be blueshifted with respect to the lower-ionization lines, such as H,, and often show a strong blue excess asymmetry not seen in the low-ionization lines. There is accumulating evidence that the H, profile is dominated by gravity, and thus provides a useful estimate of the black hole mass in AGN. The shift and asymmetry commonly seen in C iv suggest that non-gravitational effects, such as obscuration and radiation pressure, may affect the line profile. We explore the relation between the H, and C iv profiles using the ultraviolet (UV) spectra available for 81 of the 87 z, 0.5 PG quasars in the Boroson & Green sample. We find the following. (1) Narrow C iv lines (full width at half-maximum, FWHM < 2000 km s,1) are rare (,2 per cent occurrence rate) compared with narrow H, lines (,20 per cent). (2) In most objects where the H, FWHM < 4000 km s,1 the C iv line is broader than H,, but the reverse is true when the H, FWHM > 4000 km s,1. This argues against the view that C iv generally originates closer to the centre, compared with H,. (3) C iv appears to provide a significantly less accurate, and possibly biased estimate of the black hole mass in AGN, compared with H,. (4) All objects where C iv is strongly blueshifted and asymmetric have a high L/LEdd, but the reverse is not true. This suggests that a high L/LEdd is a necessary but not sufficient condition for generating a blueshifted asymmetric C iv emission. (5) We also find indications for dust reddening and scattering in ,normal' AGN. In particular, PG quasars with a redder optical,UV continuum slope show weaker C iv emission, stronger C iv absorption and a higher optical continuum polarization. [source] Simple enzymatic procedure for l -carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recyclingMICROBIAL BIOTECHNOLOGY, Issue 1 2010Jan Heyland Summary , -Peptides and their derivates are usually stable to proteolysis and have an increased half-life compared with , -peptides. Recently, , -aminopeptidases were described as a new enzyme class that enabled the enzymatic degradation and formation of , -peptides. As an alternative to the existing chemical synthesis routes, the aim of the present work was to develop a whole-cell biocatalyst for the synthesis and production of , -peptides using this enzymatic activity. For the optimization of the reaction system we chose the commercially relevant ,,, -dipeptide l -carnosine (, -alanine- l -histidine) as model product. We were able to show that different recombinant yeast and bacteria strains, which overexpress a , -peptidase, could be used directly as whole-cell biocatalysts for the synthesis of l -carnosine. By optimizing relevant reaction conditions for the best-performing recombinant Escherichia coli strain, such as pH and substrate concentrations, we obtained high l -carnosine yields of up to 71%. Long-time as well as biocatalyst recycling experiments indicated a high stability of the developed biocatalyst for at least five repeated batches. Application of the recombinant E. coli in a fed-batch process enabled the accumulation of l -carnosine to a concentration of 3.7 g l,1. [source] Melanin Offers Protection Against Induction of Cyclobutane Pyrimidine Dimers and 6,4 Photoproducts by UVB in Cultured Human Melanocytes,PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 3 2001Nico P. M. Smit ABSTRACT The goal of this investigation was to correlate the melanin content in human pigmentary cells with the generation of UVB-induced photoproducts and to examine the relationship between the melanin content and the removal of the photoproducts. Cultured melanocytes from light-skinned individuals synthesized less melanin and produced more cyclobutane pyrimidine dimers and 6,4 photoproducts upon UVB exposure than did melanocytes from black skin. Tyrosine-stimulated melanogenesis provided protection against DNA damage in both cell types. In another set of pigmented cell lines a ratio between eumelanin and pheomelanin was determined. The assessment of association between DNA damage induction and the quantity and quality of melanin revealed that eumelanin concentration correlated better with DNA protection than pheomelanin. Skin type,I and skin type,VI melanocytes, congenital nevus (CN)-derived cells and skin type,II melanocytes from a multiple-melanoma patient were grown in media with low or high l -tyrosine concentration. The cells were irradiated with 200 J/m2 UVB, and the levels of the photoproducts were determined immediately and after 6 and 24 h. Once again the induction of the photoproducts was mitigated by increased melanogenesis, and it was inversely correlated with the skin type. No significant differences were found for the removal of photoproducts in the cultures of skin types I and VI and CN cells. No indications of a delay in the removal of photoproducts in the melanocytes from the multiple-melanoma patient were found either. [source] Wastewater treatment plants as a pathway for aquatic contamination by pharmaceuticals in the Ebro river basin (Northeast Spain)ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2007Meritxell Gros Abstract The occurrence of 28 pharmaceuticals of major human consumption in Spain, including analgesics and anti-inflam-matories, lipid regulators, psychiatric drugs, antibiotics, antihistamines, and ,-blockers, was assessed along the Ebro river basin, one of the biggest irrigated lands in that country. Target compounds were simultaneously analyzed by off-line solid-phase extraction, followed by liquid chromatography-tandem mass spectrometry. The loads of detected pharmaceuticals and their removal rates were studied in seven wastewater treatment plants (WWTPs) located in the main cities along the basin. Total loads ranged from 2 to 5 and from 0.5 to 1.5 g/d/1,000 inhabitants in influent and effluent wastewaters, respectively. High removal rates (60,90%) were achieved mainly for analgesics and anti-inflammatories. The other groups showed lower rates, ranging from 20 to 60%, and in most cases, the antiepileptic carbamazepine, macrolide antibiotics, and trimethoprim were not eliminated at all. Finally, the contribution of WWTP effluents to the presence of pharmaceuticals in receiving river waters was surveyed. In receiving surface water, the most ubiquitous compounds were the analgesics and anti-inflammatories ibuprofen, diclofenac, and naproxen; the lipid regulators bezafibrate and gemfibrozil; the antibiotics erythromycin, azithromycin, sulfamethoxazole, trimethoprim, and less frequently, ofloxacin; the antiepileptic carbamazepine; the antihistamine ranitidine; and the ,-blockers atenolol and sotalol. Although levels found in WWTP effluents ranged from low ,g/L to high ng/L, pharmaceuticals in river waters occurred at levels at least one order of magnitude lower (low ng/L range) because of dilution effect. From the results obtained, it was proved that WWTP are hot spots of aquatic contamination concerning pharmaceuticals of human consumption. [source] | ||||||||||