Home About us Contact
|
Home About us Contact | ||
|
|
||
High Importance (high + importance)
Selected AbstractsAssessing cytotoxicity of (iron oxide-based) nanoparticles: an overview of different methods exemplified with cationic magnetoliposomesCONTRAST MEDIA & MOLECULAR IMAGING, Issue 5 2009Stefaan J. H. Soenen Abstract Iron oxide nanoparticles are the most widely used T2/T2* contrast agents and for biomedical research purposes, one of the main applications is the in vitro labeling of stem or therapeutic cells, allowing them to be subsequently tracked in vivo upon transplantation. To allow this, the nanoparticles used should not show any sign of cytotoxicity and not affect cellular physiology as this could impede normal cell functionality in vivo or lead to undesired side-effects. Assessing the biocompatibility of the nanoparticles has proven to be quite a difficult task. In the present work, a small overview of commonly used assays is presented in order to assess several aspects, such as cell viability, induction of reactive oxygen species, nanoparticle uptake, cellular morphology, cellular proliferation, actin cytoskeleton architecture and differentiation of stem cells. The main focus is on comparing the advantages and disadvantages of the different assays, highlighting several common problems and presenting possible solutions to these problems as well as pointing out the high importance of the relationship between intracellular nanoparticle concentration and cytotoxicity. Copyright © 2009 John Wiley & Sons, Ltd. [source] Self-rated importance of religion predicts one-year outcome of patients with panic disorderDEPRESSION AND ANXIETY, Issue 5 2006F.R.C.P.(C.), Rudy Bowen M.D.C.M. Abstract Cognitive-behavioral therapy and medication are efficacious treatments for panic disorder, but individual attributes such as coping and motivation are important determinants of treatment response. A sample of 56 patients with panic disorder, treated with group cognitive-behavioral therapy, were reassessed 6 months and 12 months after initial assessment. We studied the effect of self-rated importance of religion, perceived stress, self-esteem, mastery, and interpersonal alienation on outcome as measured by the General Severity Index of the Brief Symptom Inventory (BSI.GSI). Importance of religion was a predictor of BSI.GSI symptom improvement at 1 year. Over time, improvement was seen for the religion is very important subgroup in the BSI.GSI and Perceived Stress Scales. This study suggests that one mechanism by which high importance of religion reduces psychiatric symptoms is through reducing perceived stress. Depression and Anxiety 23:266,273, 2006. © 2006 Wiley-Liss, Inc. [source] Public attitudes towards the environmental impact of salmon aquaculture in ScotlandENVIRONMENTAL POLICY AND GOVERNANCE, Issue 2 2006David Whitmarsh Abstract This paper reports the results of a survey of public attitudes towards aquaculture, using salmon farming in Scotland as a case study. The aims have been to identify the priority that people attach to the environmental performance of the salmon aquaculture industry, relative to other objectives, and to measure the economic benefits to society from salmon farmed using methods that cause less organic pollution. The results indicate that the public attach a relatively high importance to minimizing environmental damage from aquaculture, and this has its parallel in the finding that people are willing to pay a price premium for salmon produced in a more environmentally benign way. Copyright © 2006 John Wiley & Sons, Ltd and ERP Environment. [source] Perovskite BaHfO3 Dielectric Layers for Dynamic Random Access Memory Storage Capacitor Applications,ADVANCED ENGINEERING MATERIALS, Issue 4 2009Grzegorz Lupina Very thin layers of dielectric materials are of high importance for many advanced microelectronic applications. In this work, properties of very thin (<50,nm) BaHfO3 layers prepared using molecular beam deposition and chemical vapor deposition are examined. We conclude that polycrystalline cubic BaHfO3 films appear to be promising candidates for future memory capacitor applications. [source] Effects of high-magnitude/low-frequency fluvial events generated by intense snowmelt or heavy rainfall in arctic periglacial environments in northern Swedish Lapland and northern SiberiaGEOGRAFISKA ANNALER SERIES A: PHYSICAL GEOGRAPHY, Issue 1 2004A. A. Beylich Abstract In the Latnjavagge drainage basin (68°21,N, 18°29,E), an arctic-oceanic periglacial environment in northernmost Swedish Lapland, the fluvial sediment transport and the characteristics and importance of high-magnitude/low-frequency fluvial events generated by intense snowmelt or heavy rainfall have been investigated and compared with snowmelt- and rainfall-induced discharge peaks in the Levinson-Lessing Lake basin (Krasnaya river system) on the Taimyr Peninsula, an arctic periglacial environment in northern Siberia (74°32,N, 98°35,E). In Latnjavagge (9 km2) the intensity of fluvial sediment transport is very low. Most of the total annual sediment load is transported in a few days during snowmelt generated runoff peaks. Due to the continuous and very stable vegetation covering most areas below 1300 m a.s.l. in the Latnjavagge catchment, larger rainfall events are of limited importance for sediment transport in this environment. Compared to that, in the c. 40 times larger Krasnaya riversystem rainfall-generated runoff peaks cause significant sediment transport. The main sediment sources in the Latnjavagge drainage basin are permanent ice patches, channel debris pavements mobilized during peak discharges and exposing fines, and material mobilized by slush-flows. In the Krasnaya river system river bank erosion is the main sediment source. In both periglacial environments more than 90% of the annual sediment yield is transported during runoff peaks. The results from both arctic periglacial environments underline the high importance of high-magnitude/low-frequency fluvial events for the total fluvial sediment budgets of periglacial fluvial systems. Restricted sediment availability is in both arctic environments the major controlling factor for this behaviour. [source] The amazing universe of hepatic microstructure,HEPATOLOGY, Issue 2 2009Valeer J. Desmet An informal review is presented by the author of his 50 years of involvement in practice and research in hepatopathology. Some background for the author's attitude and meandering pathway into his professional career serves as introduction to a short discussion of the main topics of his interest and expertise. Histogenesis of liver cancer was the theme of early work for a Ph.D. thesis, the results of which were lost into oblivion due to local rules and circumstances, but were rescued three decades later. His conclusions about the cells of origin of liver cancer remain concordant with the newer concepts in the field after nearly half a century. Studies in the field of chronic hepatitis became a long saga, involving the first classification of this syndrome by "the Gnomes" in 1968, histochemical investigations of viral antigens, lymphocyte subsets and adhesion molecules, and a quarter century later, the creation of a new classification presently in use. Cholestasis was a broadening field in diagnostic entities and involved the study of liver lesions, comprising pathways of bile regurgitation (including reversed secretory polarity of hepatocytes) and so-called ductular reaction. The latter topic has a high importance for the various roles it plays in modulating liver tissue of chronic cholestasis into biliary cirrhosis, and as the territory of hepatic progenitor cells, crucial for liver regeneration in adverse conditions and in development of liver cancer. Study of the embryology of intrahepatic bile ducts helped to clarify the strange appearance of the ducts in "ductal plate configuration" in several conditions, including some forms of biliary atresia with poor prognosis and all varieties of fibrocystic bile duct diseases with "ductal plate malformation" as the basic morphologic lesion. (HEPATOLOGY 2009;50:333,344.) [source] Patient expectation and satisfaction with nursing care in Turkey: a literature reviewINTERNATIONAL NURSING REVIEW, Issue 3 2007S. A. Özsoy phd Background:, Patient satisfaction is used as an important indicator of care quality and is frequently included in healthcare planning and evaluation. This study presents a review of research on patient expectation and satisfaction with nursing care in Turkey. Aim:, To review the national literature on patient expectations and satisfaction with nursing care. Methods:, The researchers reviewed 3089 articles of which 27 were discussed in detail. Of these 3089 articles, 1812 were from all issues of 14 Turkish nursing journals which have been published in the last 50 years, and 1277 articles were from 24 nursing congress and symposium books. Results:, The results of this study are divided into two categories: expectations concerning ,nursing care', and ,satisfaction with nursing care'. The findings show that there exist conceptual and philosophic deficiencies in the approaches to patient satisfaction and that there is a need to use standardized instruments to study and assess patient satisfaction in the future. Conclusion:, Over the last 20 years, studies of patient expectation and satisfaction with nursing care have been gaining high importance, but it is also noticed that in a national and philosophical approach the studies are not based on clearly defined concepts. Moreover, it has been concluded that there is a need for valid and reliable tools in the evaluation of satisfaction. [source] The health promotion model as assessed by ageing workersJOURNAL OF CLINICAL NURSING, Issue 2 2006Paula Naumanen PhD Aims., This study describes qualitatively ageing workers and their health promotion. It also describes quantitatively the importance of individual, workplace and occupational health promoting factors, the impact of health promotion and the contributions of the other co-partners promoting the health of ageing workers. These form the foundation for a model of health promotion. Background., Very little is known about the health promotion factors exclusively focused at ageing workers. Methods., A pretested questionnaire with structured and some open-ended questions were devised to form the basis of qualitative data and sent to 150 ageing workers, of whom 93 (62%) returned it. Qualitative data were analysed by content analysis. Statistical analyses were performed using frequencies and means. Results., Over 90% of respondents stated that health habits, individual arrangements at the workplace, a good workplace spirit, health checks, counselling and access to nursing care were important factors for health promotion. Better health (99%), work satisfaction (95%) and motivation of employees (96%) were the factors impacting on health promotion. Ageing workers stated that health and safety organization (94%) and rehabilitation institutes (93%) were the most important co-partners. Conclusions., This study confirms extremely high importance of different health promotion factors, their impact and some co-partners. Health problems, early retirement and work absenteeism are rather common in ageing workers. If we are to avoid these problems, it is important to support their work ability effectively and systematically to allow these workers to stay employed until the normal retirement age. Relevance to clinical practice., It is necessary, that management appreciate the benefits of ageing workers; they have to take a positive attitude toward these workers and participate more effectively in their health promotion. Occupational health professionals play a key role in training workers and management to undertake the required measures. [source] Performance comparisons and attachment: An investigation of competitive responses in close relationshipsPERSONAL RELATIONSHIPS, Issue 3 2005ANTHONY SCINTA Two studies investigated whether affective responses to competitive performance situations are moderated by attachment style. In Study 1, participants (n= 115) imagined their reactions to a superior or inferior performance against their romantic partner or an acquaintance. Results showed that participants low in attachment avoidance, relative to those high in avoidance, indicated more positivity after an inferior performance (empathy effect) to their partners, and this finding held only in domains of high importance to the partner. In Study 2, participants (n= 53) imagined comparisons with their partner or a close friend. Low-avoidance participants, relative to high-avoidance participants, exhibited sympathy and empathy effects in comparisons involving their romantic partner but not those involving a friend. The findings are discussed in terms of one's model of other and perceived self,other separation, which are defined by avoidance but not anxiety. [source] Was Ökonomen lesen und schätzen: Ergebnisse einer UmfragePERSPEKTIVEN DER WIRTSCHAFTSPOLITIK, Issue 2 2001Michael Bräninger This paper summarizes the main results of a survey, which we conducted in May 2000 among German-speaking economists about their views on economics publications. Our results indicate that the world market for economics publications is not fully integrated across countries, but it appears to be at least partially segmented. Economics journals published in German-speaking countries appear to be relatively important for the work of German-speaking economists even though other journals have a better reputation among them. And although internationally reputed journals enjoy the highest reputation among German-speaking economists, domestic economic journals enjoy a higher reputation than would be justified according to international citation-based rankings. Especially older German-speaking economists tend to attach relatively high importance and reputation to domestic journals, while younger economists tend to favour international journals that focus on certain subfields of economics. [source] Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian LakesPHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 4 2010Beatriz Modenutti Through laboratory experiments, we tested whether UV radiation (UVR) induces filamentation in natural bacteria assemblages from North Andean Patagonian lakes. We incubated water from three different lakes for 72 h in four separate treatments: (1) UVR + PAR (photosynthetically active radiation), (2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. The irradiance levels used in the experiments were equivalent to those registered at the epilimnion of the lakes. In the UVR treatments filamentation was induced after the first 24 h and the proportion continued to increase for the next 48,72 h. A comparison of the gross composition and diversity of the entire community (cells >0.2 ,m) with bacterial filaments alone (>5.0 ,m) showed that UVR-induced filamentation is not a feature of any particular cluster. By sequencing part of the 16S rRNA gene of the taxonomic units obtained using denaturing gels, we observed that strains in the ,-Proteobacteria group were of relatively high importance in filament formation, followed by Cytophaga,Flavobacterium,Bacteroides, ,-Proteobacteria and ,-Proteobacteria, whereas Actinobacteria were almost nonexistent in the filaments. We propose that UVR doses equivalent to those of Andean lakes produce bacterial morphological changes, and that all bacterial groups except Actinobacteria can potentially form filaments. [source] Flammability and fire resistance of composites reinforced by natural fibersPOLYMERS FOR ADVANCED TECHNOLOGIES, Issue 6 2008Ryszard Koz, owski Abstract Natural fiber-reinforced composites are more and more frequently applied to building industry and transportation. Therefore, the knowledge of their behavior during fire is of high importance. Flammability is one of very important parameters that often limits the application of composites to a given area. It is well-known that addition of lignocellulosic fibers to polymer changes mechanical properties of the product obtained. However, little information is available on their fire performance. The purpose of this review was to obtain fire performance data for several types of composites reinforced by lignocellulosic fibers. Copyright © 2008 John Wiley & Sons, Ltd. [source] A multiphase finite element simulation of biological conversion processes in landfillsPROCEEDINGS IN APPLIED MATHEMATICS & MECHANICS, Issue 1 2009Tim Ricken Worldwide, landfills are the most common way to dispose of waste, but have an impact on the environment as a result of harmful gas and leachate production. Estimating the long-term behaviour of a landfill in regard to this gas production and organic degrading, as well as to settlement and waste water production, is of high importance. Therefore, a model has been developed to simulate these processes. This constitutive model is based on the multiphase Theory of Porous Media. The body under investigation consists of an organic and an inorganic phase as well as a liquid and a gas phase. The equations of the model are developed on the basis of a consistent thermo-mechanical approach including the momentum balance for the solid phase and the mixture, the energy balance for the mixture and the mass balance for the gas phase. All interactions between the constituents such as mass transfers, interaction forces and energy fluxes are taken into consideration. The strongly coupled set of partial differential equations is implemented in the finite element code FEAP. The theoretical framework and the results of meantime successfully performed simulation of a real landfill body will be shown. (© 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Reevaluation of the Phylogenetic Relationship between Mobilid and Sessilid Peritrichs (Ciliophora, Oligohymenophorea) Based on Small Subunit rRNA Genes SequencesTHE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 5 2006YING-CHUN GONG ABSTRACT. Based on morphological characters, peritrich ciliates (Class Olygohymenophorea, Subclass Peritrichia) have been subdivided into the Orders Sessilida and Mobilida. Molecular phylogenetic studies on peritrichs have been restricted to members of the Order Sessilida. In order to shed more light into the evolutionary relationships within peritrichs, the complete small subunit rRNA (SSU rRNA) sequences of four mobilid species, Trichodina nobilis, Trichodina heterodentata, Trichodina reticulata, and Trichodinella myakkae were used to construct phylogenetic trees using maximum parsimony, neighbor joining, and Bayesian analyses. Whatever phylogenetic method used, the peritrichs did not constitute a monophyletic group: mobilid and sessilid species did not cluster together. Similarity in morphology but difference in molecular data led us to suggest that the oral structures of peritrichs are the result of evolutionary convergence. In addition, Trichodina reticulata, a Trichodina species with granules in the center of the adhesive disc, branched separately from its congeners, Trichodina nobilis and Trichodina heterodentata, trichodinids without such granules. This indicates that granules in the adhesive disc might be a phylogenetic character of high importance within the Family Trichodinidae. [source] Implications of recreational fishing for elasmobranch conservation in the Great Barrier Reef Marine ParkAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 3 2010Ann-Maree J. Lynch Abstract 1.309 Great Barrier Reef Marine Park recreational fishers were surveyed to examine recreational catch and harvest of elasmobranchs and to explore recreational fishers' handling behaviour and attitudes. 2.Elasmobranchs represented 6% of fishers' total catch of all fish (including released individuals), and 0.8% of fishers' total harvest (i.e. retained individuals) across all survey days. The majority of elasmobranchs caught by fishers were released, primarily because they were perceived as being inedible. 3.Recreational fishers' self-reported handling and release behaviour for elasmobranchs is largely consistent with ,best practice' guidelines except that fishers had low use of circle hooks and barbless hooks, and a significant proportion (33%) reported using stainless steel hooks. 4.Most fishers had positive attitudes towards elasmobranchs, placing high importance on releasing sharks and rays in good condition (86%), high value on their existence (84%), and low value on catching them (63%). 5.Results indicate that post-release mortality is probably the largest source of recreational fishing mortality of elasmobranchs in the Great Barrier Reef. Future research should be targeted at obtaining better estimates of species-specific post-release mortality levels, understanding how post-release survival can be increased by changing fishing techniques or fisher behaviour, and developing more effective methods of engaging fishers in elasmobranch conservation. Copyright © 2009 John Wiley & Sons, Ltd. [source] HI , the window to the early universe in X-raysASTRONOMISCHE NACHRICHTEN, Issue 1-2 2003J. Kerp Abstract A detailed understanding of the soft X-ray background (SXRB) is of high importance for the next generation of X-ray telescopes, which will focus on early universe objects. Because of their high redshift the characteristic X-ray emission of the early universe objects will be observable in the soft X-ray energy domain below E = 1 keV. In this energy regime the photoelectric absorption of the galactic interstellar medium attenuates the X-ray emission most strongly. The confusion with the spatially highly variable galactic soft X-ray emission might be an additional severe problem to disentangle the emission of the early universe object and the SXRB. We present the cross correlation of the Leiden/Dwingeloo HI 21-cm line survey with the ROSAT all-sky survey. The analyses disclose the existence of a single temperature plasma within the Milky Way halo. The strength of the photoelectric absorption is quantitatively traced by the distribution of the HI emission across the whole sky. Both findings in combination open the window to the highly redshift early universe objects. [source] Bauten in deutschen Erdbebengebieten , zur Einführung der DIN 4149: 2005BAUTECHNIK, Issue 8 2005Jochen Schwarz Dr.-Ing. Die Einführung der DIN 4149: 2005 bildet eine wichtige Voraussetzung, um im Kontext der Harmonisierung europäischer Baubestimmungen ein erdbebensicheres und wirtschaftliches Bauen in den Erdbebengebieten Deutschlands zu ermöglichen. Verschiedene Phasen der Erarbeitung werden in einer chronologischen Form beleuchtet und mit einem Ausblick auf die weitere Normenentwicklung abgeschlossen. Es werden interdisziplinäre Forschungsarbeiten gewürdigt, die wesentlich dazu beigetragen haben, das Regelwerk der seismischen Lastannahmen für Bauwerke neu zu konzipieren. Insbesondere durch die Festlegung geologie- und untergrundbezogener Bemessungsspektren wird eine differenzierte Beschreibung seismischer Einwirkungen und auf die regionalen Besonderheiten deutscher Erdbebengebiete ausgerichtete Bauwerksbemessung gewährleistet. Der erreichte Grad der Harmonisierung europäischer Erdbebenbaunormen wird anhand aktueller Zonenkarten sowie der Festlegungen zu Bemessungsbeschleunigungen entlang der nationalen Grenzen beispielhaft nachvollzogen. Da die neue Gefährdungszonenkarte eine veränderte regionale Verteilung der Erdbebenzonen bedingt, wird auf die Notwendigkeit einer Bewertung der Erdbebentauglichkeit der vorherrschenden Bauweisen und die Identifikation der im Katastrophenfall bedeutenden Anlagen und Einrichtungen hingewiesen. Buildings in German seismic regions , to the introduction of the revised German Seismic Code DIN 4149: 2005. The introduction of the revised Seismic Code DIN 4149: 2005 can be regarded as an important step towards earthquake resistant and economic building design in German earthquake regions. Different stages of its elaboration are described chronologically, concluding in an outlook on the ongoing development in European code standardization. Interdisciplinary research projects, which influenced the redrafting of the general rules and the implementation of new approaches, substantially, are recognized for their contributions. In particular, the concept of geology- and subsoil-dependent response spectra enables a refined description of seismic action and building design while reflecting the existing site conditions realistically. The attained state of harmonisation of national regulations is discussed exemplary by comparing peak ground and derived design accelerations alongside the borders with neighbouring countries like Belgium, France and Switzerland. With respect to the new zoning map and the modifications of affected areas, the need of practical implementation is directed towards two tasks: evaluation of the existing building stock and the predominant building types and identification of those structures and facilities which are of high importance in cases of catastrophic events. [source] What constitutes optical warning signals of ladybirds (Coleoptera: Coccinellidae) towards bird predators: colour, pattern or general look?BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 1 2009MICHAELA DOLENSKÁ Most ladybirds (Coleoptera: Coccinellidae) possess chemical protection against predators and signal its presence by less or more conspicuous coloration, which can be considered as a warning. Most ladybirds possess a dotted pattern, althougn the number, shape, and size of the spots, as well as their colour, varies considerably. Almost all ladybirds have a characteristic general appearance (body shape). We considered these traits to be used in ladybird recognition by avian predators. In the present study, we compared the reactions of avian predators (Parus major) caught in the wild, to four differently coloured ladybird beetles (Coccinella septempunctata, Exochomus quadripustulatus, Subcoccinella vigintiquatuorpunctata, and Cynegetis impunctata) and two artificial modifications of C. septempunctata; the first was deprived of their elytral spotted pattern by painting it brown, the other had their elytra removed (i.e. altering their general ladybird appearance). Ladybirds with a spotted pattern were attacked less frequently than unspotted ones. Ladybirds with removed elytra were attacked much more often than any ladybird with a preserved general appearance. The results obtained in the present study suggest the high importance of the spotted pattern as well as general appearance in the ladybird recognition process. Additional experiments with naïve birds (hand-reared P. major) demonstrated the innateness of the aversion to two differently spotted ladybird species (C. septempunctata and Scymnus frontalis). © 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 98, 234,242. [source] A delay in radical nephroureterectomy can lead to upstagingBJU INTERNATIONAL, Issue 6 2010Matthias Waldert Study Type , Prognosis (case series) Level of Evidence 4 OBJECTIVE To examine the association between the delay from diagnosis of upper-tract urothelial carcinoma (UTUC) to radical nephroureterectomy (RNU), and the pathological features and outcomes, as the decision to proceed to RNU for an individual patient is complex. PATIENTS AND METHODS The records of 187 patients who had RNU were reviewed; the interval from diagnosis to RNU was analysed as both a continuous (months) and categorical variable (<3 vs ,3 months). Logistic regression and survival analyses were used to evaluate the association between time from diagnosis to RNU with pathological characteristics and clinical outcomes. RESULTS The median time from diagnosis to RNU was 45 days (interquartile range 68). A delay from diagnosis to RNU analysed as a continuous variable was associated with advanced stage, higher grade, previous endoscopic procedure, tumour necrosis, infiltrative tumour architecture, and lymphovascular invasion (P = 0.034), but not disease recurrence or cancer-specific mortality. In the subgroup of patients (90, 48.1%) who had muscle-invasive disease (,pT2) a longer delay from diagnosis to RNU as a continuous variable was associated with advanced stage (P = 0.030), higher grade (P = 0.014), infiltrative tumour architecture (P = 0.044), lymphovascular invasion (P = 0.034), disease recurrence (P = 0.02), and cancer-specific mortality (P = 0.03). CONCLUSIONS Our data suggest that a delay in the interval from diagnosis to RNU is associated with more advanced disease stage. These findings might have important implications for trial design in the ongoing evaluation of neoadjuvant regimens. Timely consideration of definitive treatment for patients with high-risk UTUC is of high importance. Further studies are necessary to validate these hypothesis-generating findings. [source] 1362: Infectious posterior uveitisACTA OPHTHALMOLOGICA, Issue 2010N MARKOMICHELAKIS Several infectious agents (parasites, bacteria, fungi, and viruses) can invade the eye and lead to ocular inflammation. Infectious causes should always be considered and ruled out in all patients with posterior uveitis. Toxoplasma gondii is by far the most common cause of infectious posterior uveitis in all ages, while Toxocara canis infects typically children. Onchocerciasis and other parasitic diseases may also cause posterior uveitis, more commonly in developing countries. Nowadays, old bacterial diseases, tuberculosis and syphilis, emerged as common causes of posterior uveitis. Bartonella henselae and Borrelia burgdorferi are other bacteria that can also cause posterior uveitis. Fungal posterior uveitis usually occurs in immunosuppressed patients or in intravenous drug users. Herpes viruses (HSV, VZV, CMV) are also associated with infectious retinitis, in immunocompromised as well as immunocompetent patients, with quite destructive clinical course. Recently, more viruses (such as West Nile virus, Rift valley fever, dengue fever, and chikungunya) have been recognized as etiologic factors of posterior infectious uveitis. A rapid and accurate diagnosis is of high importance for the successful treatment and visual outcome of infectious uveitis. Techniques and methods, such PCR and detection of specific antibodies in the intraocular fluids (Goldmann-Witmer coefficient), play a key role towards the detection of the pathogen of posterior infectious uveitis. [source] Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single-Silver-Nanoparticle CountingCHEMISTRY - A EUROPEAN JOURNAL, Issue 3 2010Fagong Xu Dr. Abstract DNA and RNA analysis is of high importance for clinical diagnoses, forensic analysis, and basic studies in the biological and biomedical fields. In this paper, we report the ultrahighly sensitive homogeneous detection of DNA and microRNA by using a novel single-silver-nanoparticle counting (SSNPC) technique. The principle of SSNPC is based on the photon-burst counting of single silver nanoparticles (Ag NPs) in a highly focused laser beam (about 0.5,fL detection volume) due to Brownian motion and the strong resonance Rayleigh scattering of single Ag NPs. We first investigated the performance of the SSNPC system and then developed an ultrasensitive homogeneous detection method for DNA and microRNA based on this single-nanoparticle technique. Sandwich nucleic acid hybridization models were utilized in the assays. In the hybridization process, when two Ag-NP,oligonucleotide conjugates were mixed in a sample containing DNA (or microRNA) targets, the binding of the targets caused the Ag NPs to form dimers (or oligomers), which led to a reduction in the photon-burst counts. The SSNPC method was used to measure the change in the photon-burst counts. The relationship between the change of the photon-burst counts and the target concentration showed a good linearity. This method was used for the assay of sequence-specific DNA fragments and microRNAs. The detection limits were at about the 1,fM level, which is 2,5 orders of magnitude more sensitive than current homogeneous methods. [source] Unmet education and training needs in adolescent health of health professionals in a UK children's hospitalACTA PAEDIATRICA, Issue 6 2006Janet E. McDonagh Abstract Aim: To determine the perceived education and training needs in adolescent health of health professionals. Design: Cross-sectional survey Setting and subjects: Hospital staff in a UK children's hospital. Outcome measures: perceived barriers, confidence, knowledge, skill and prior teaching in key adolescent health subject areas. Results: The hospital survey was completed by 159/1400 professionals representing a completion rate of 11%. Doctors and staff from ,Professions allied to medicine' rated ,lack of training', ,lack of teaching materials' and ,lack of community resources' as the main barriers to providing developmentally appropriate care. Sixty per cent of hospital respondents had received no prior specific training in adolescent health. All but four topics were perceived to be of very high or high importance by the majority of respondents (54,90%). Low scores in perceived knowledge, confidence and/or skill were reported in nine key subject areas (including adolescent mental health and substance use). Differences between doctors and professions allied to medicine were observed in a minority of areas. Conclusion: Unmet education and training needs of a range of professionals working in a paediatric setting were identified in key areas of adolescent health and they provide useful directions for the development of future multidisciplinary training programmes. [source] | |||||||||||||||||||||||||