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Histamine Release Test (histamine + release_test)
Selected AbstractsHistamine release test and measurement of antigen‐specific IgE antibody in the diagnosis of allergic conjunctival diseasesJOURNAL OF CLINICAL LABORATORY ANALYSIS, Issue 2 2001Eiichi Uchio Abstract Although systemic allergic laboratory tests for the quantification of allergen‐specific serum IgE antibody have been widely used, in these tests a high titer of serum specific IgE does not necessarily indicate evidence of allergy. We evaluated the diagnostic value of the glass microfiber‐based histamine release test (HRT) using small amounts of whole blood, in 36 cases of allergic conjunctival diseases: 17 cases of allergic conjunctivitis and 19 of atopic keratoconjunctivitis. The patients were evaluated by HRT, capsulated hydrolic carrier polymer (CAP)‐RAST, and conjunctival provocation test (CPT) against ten allergens. The positive rates for all allergens were higher in CAP‐RAST than in HRT. The mean concordance of HRT with CAP‐RAST results was 0.789. The mean concordance of HRT with CPT was 0.892 and that of CAP‐RAST with CPT was 0.693. A significantly higher concordance was observed in HRT than CAP‐RAST for Japanese cedar and mite antigen. The mean sensitivity, specificity, and efficiency of HRT were higher than those of CAP‐RAST. These results indicate that CAP‐RAST is good for the screening of allergens and that HRT has an advantage in the confirmation of clinical allergens in allergic conjunctival diseases because of its high sensitivity, specificity, efficiency, and higher concordance with CPT. J. Clin. Lab. Anal. 15:71–75, 2001. © 2001 Wiley‐Liss, Inc. [source] Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venomCYTOMETRY, Issue 1 2003C. Lambert Abstract Background Flow cytometry (FCM) has been proposed for specific allergy in vitro testing. We investigated its biological significance for allergy to Hymenoptera venoms and compared it with the routinely performed basophil histamine release test (HRT). Methods Blood samples from 26 allergic and 8 nonallergic donors were incubated with venom at serial concentrations. Basophils were analyzed with anti-CD45-PE-Cyanin 5, Anti-IgE-FITC, and Anti-CD63-Phycoerythrine. HRT was measured by radioimmunoassay. Results FCM was as convenient as HRT for measuring basophil reactivity in at least 87% of allergic and 75% of nonallergic subjects. CD63 outer expression was specifically induced in 91% of releaser subjects (86% on HRT) and in 1 of 10 tests in nonallergic donors, or one of six tests (16% on HRT) in allergic patients tested with an irrelevant allergen. Both methods were concordant in 85.7% of the tests. The three discordant patients had low-grade reactions and borderline biological responses on FCM (n = 2) or HRT (n = 1). Conclusions The dynamic, physiologic significance of CD63, the dose,response curve, and dependency on ethylene-diaminetetra acetic acid suggested that both tests reflect the same mechanism. Cytometry Part B (Clin. Cytometry) 52B:13,19, 2003. © 2003 Wiley-Liss, Inc. [source] Semi-purification of the immunoglobulin E-sweat antigen acting on mast cells and basophils in atopic dermatitisEXPERIMENTAL DERMATOLOGY, Issue 4 2006A. Tanaka Background:, Sweating aggravates the symptoms of atopic dermatitis (AD). We have recently reported positive skin reactions and histamine release from basophils in response to autologous sweat in patients with AD. Objective:, To characterize the biochemical and immunological properties of the substance in sweat that evokes histamine release and to study the usability of the basophil-histamine release test with the sweat antigen for AD. Methods:, Sweat collected from healthy volunteers was purified using chromatographies. Serum immunoglobulin (Ig)E of four patients with AD were purified using an affinity-chromatography column with anti-IgE antibodies. The amount of semi-purified sweat antigen (138 ng protein/ml) that induced a half-maximum reaction of basophils of a patient with AD was utilized for the basophil histamine release test. The involvement of specific IgE and high-affinity IgE receptor (Fc,RI) in the reactions was examined using basophils of healthy volunteers, a human mast cell line (LAD2), and a rat basophilic leukemia cell line transfected with human ,-subunit of Fc,RI (RBL-48). Results:, The semi-purified sweat antigen induced histamine release from the basophils of 47 of 61 (74.6%) patients with AD and four of 46 (8.7%) healthy controls. Both basophils and mast cells sensitized with the patient-derived IgE showed degranulation upon stimulation with the sweat antigen. However, no reaction was observed when cells were sensitized with myeloma IgE or the antigen was treated with proteases. Conclusion:, The semi-purified standardized sweat antigen consists of a protein that induces degranulation of basophils and mast cells via antigen-specific IgE and Fc,RI in patients with AD. [source] Histamine release test and measurement of antigen‐specific IgE antibody in the diagnosis of allergic conjunctival diseasesJOURNAL OF CLINICAL LABORATORY ANALYSIS, Issue 2 2001Eiichi Uchio Abstract Although systemic allergic laboratory tests for the quantification of allergen‐specific serum IgE antibody have been widely used, in these tests a high titer of serum specific IgE does not necessarily indicate evidence of allergy. We evaluated the diagnostic value of the glass microfiber‐based histamine release test (HRT) using small amounts of whole blood, in 36 cases of allergic conjunctival diseases: 17 cases of allergic conjunctivitis and 19 of atopic keratoconjunctivitis. The patients were evaluated by HRT, capsulated hydrolic carrier polymer (CAP)‐RAST, and conjunctival provocation test (CPT) against ten allergens. The positive rates for all allergens were higher in CAP‐RAST than in HRT. The mean concordance of HRT with CAP‐RAST results was 0.789. The mean concordance of HRT with CPT was 0.892 and that of CAP‐RAST with CPT was 0.693. A significantly higher concordance was observed in HRT than CAP‐RAST for Japanese cedar and mite antigen. The mean sensitivity, specificity, and efficiency of HRT were higher than those of CAP‐RAST. These results indicate that CAP‐RAST is good for the screening of allergens and that HRT has an advantage in the confirmation of clinical allergens in allergic conjunctival diseases because of its high sensitivity, specificity, efficiency, and higher concordance with CPT. J. Clin. Lab. Anal. 15:71–75, 2001. © 2001 Wiley‐Liss, Inc. [source] Sweat antigen induces histamine release from basophils of patients with cholinergic urticaria associated with atopic diathesisBRITISH JOURNAL OF DERMATOLOGY, Issue 2 2009S. Takahagi Summary Background, We previously demonstrated that the semipurified human sweat antigen causes skin reactions and histamine release from basophils via specific IgE in patients with atopic dermatitis (AD). Patients with cholinergic urticaria (ChU) also develop skin reactions and histamine release of basophils in response to autologous sweat. Objectives, To study whether or not patients with ChU share sensitivity for the sweat antigen with patients with AD and to study the clinical characteristics among patients with ChU and the relationship with histamine-release activity of basophils. Methods, The sweat antigen that induces histamine release from basophils of patients with AD was prepared by Con-A, anion-exchange and reverse-phase chromatography. Relationships between histamine-release activity against the sweat antigen and clinical features of patients with ChU were analysed. Results, Twenty-three of 35 patients with ChU showed > 5% net histamine release in response to the semipurified sweat antigen, whereas none of healthy controls did so. In patients with ChU, histamine release in response to semipurified sweat antigen significantly correlated with the level of serum IgE and eosinophil numbers in peripheral blood. Incidence of each atopic disease in patients with ChU tended to be higher than in the general Japanese population. When the patients were categorized according to their responses in the histamine release test, the positive group tended to show a higher incidence of AD and bronchial asthma compared with the negative group. Conclusions, ChU and AD may share hypersensitivity to common antigens in sweat. The sweat allergy and atopic diathesis are associated with each other. [source] Biological characterization of glutaraldehyde-modified Parietaria judaica pollen extractsCLINICAL & EXPERIMENTAL ALLERGY, Issue 2 2004I. Ibarrola Summary Background Allergoids are widely used in specific immunotherapy (SIT) for the treatment of IgE-mediated allergic diseases, but all techniques for standardization of conventional allergic extracts may not be appropriate for standardization of a glutaraldehyde (GA)-modified extract because of the unique characteristics of these extracts. Objective To assess an accurate methodology for standardization of chemically modified extracts. Methods GA-modified extracts from Parietaria judaica pollen were purified by diafiltration. Biochemical properties were investigated by determination of amino groups, chromatography, and SDS-PAGE. The IgE-binding activity was determined by skin prick test, enzyme allergosorbent test inhibition, basophil activation, and histamine release tests. Peripheral blood mononuclear cells (PBMCs) from P. judaica pollen-allergic subjects were stimulated with either native or allergoid extracts, and proliferation was measured. Results Biochemical data indicated a high degree of allergen polymerization resulting in extract components higher than 100 kDa. IgE-binding activity, both in vivo and in vitro, was reduced by more than 99.8%. Both allergen and allergoid induced PBMC proliferation and synthesis of blocking IgG antibodies at similar rates. Moreover, no evidence of introduction of new determinants by chemical modification was found. Conclusions The preparation of GA-modified extracts by diafiltration is faster and more reliable than previous chromatographic methods. These modified extracts have drastically reduced their allergenicity while maintaining their immunogenicity, and therefore they can be used in safer and shortened schedules of SIT. [source] Basophil histamine release tests in the diagnosis of allergy and asthmaCLINICAL & EXPERIMENTAL ALLERGY, Issue 3 2001A. D. Crockard [source] | ||||||||||