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Amylase Inhibitory Activity (amylase + inhibitory_activity)

Distribution by Scientific Domains
Chemistry75%

Selected Abstracts

In Vitro,Potential of,Ascophyllum nodosum,Phenolic Antioxidant-Mediated ,-Glucosidase and ,-Amylase Inhibition

JOURNAL OF FOOD SCIENCE, Issue 3 2010
E. Apostolidis
ABSTRACT:,Ascophyllum nodosum,is a brown seaweed that grows abundantly in the Northeast coastal region. In this study, the potential of,A. nodosum,for type 2 diabetes management through antioxidant-mediated ,-glucosidase and ,-amylase inhibition was investigated. After the initial screening of 4 locally harvested seaweeds,,A. nodosum,was chosen for its highest phenolic content and was subjected to water extraction. Among extraction ratios of 50 g to 100 to 1000 mL at room temperature, 50 g/400 mL yielded the highest phenolic content of 4.5 mg/g wet weight. For evaluation of extraction temperature ranging from 20 to 80 °C, 50 g/400 mL was chosen as a minimum amount of extractant. Among temperatures studied, extraction at 80 °C resulted in the highest total phenolic contents (4.2 mg/g wet weight). All extracts had similar levels of antioxidant activity in the range of 60% to 70% in terms of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The 80 °C extract had the highest ,-glucosidase and ,-amylase inhibitory activity with IC50 of 0.24 and 1.34 ,g phenolics, respectively, compared to the IC50 of acarbose, reference inhibitor, being 0.37 and 0.68 ,g. The results show that fresh,A. nodosum,has strong ,-glucosidase and mild ,-amylase inhibitory activities that correlated with phenolic contents. This study suggests a nutraceutical potential of,A. nodosum,based on phytochemical antioxidant and antihyperglycemia activities. [source]

INHIBITORY POTENTIAL OF WINE AND TEA AGAINST ,-AMYLASE AND ,-GLUCOSIDASE FOR MANAGEMENT OF HYPERGLYCEMIA LINKED TO TYPE 2 DIABETES

JOURNAL OF FOOD BIOCHEMISTRY, Issue 1 2008
YOUNG-IN KWON
ABSTRACT Natural ,-amylase and ,-glucosidase inhibitors from food-grade plants offer an attractive strategy to manage postprandial hyperglycemia for type 2 diabetes management via control of starch breakdown and intestinal glucose absorption. In this study, four random sources of red and white wines as well as four types of teas were investigated for ,-amylase and ,-glucosidase inhibitory potential. Water extracts of black tea had the highest ,-glucosidase inhibitory activity, followed by white tea and oolong tea. All the randomly selected red wines had significant ,-glucosidase inhibitory activity compared to white wine. The ,-glucosidase inhibitory activity of the tea and wines correlated to the phenolic content, antioxidant activity and phenolic profile of the extracts. Further, these extracts had less or no ,-amylase inhibitory activity, indicating potential to overcome the side effects of undigested starch. This research has relevance for managing hyperglycemia and related oxidation-linked dysfunction and concurrently reducing problems of undigested starch. PRACTICAL APPLICATIONS In this study anti-diabetic-relevant potential of wines and teas were confirmed in four types of red and white wines as well as four types of commonly available teas using in vitro enzyme assays for alpha-glucosidase and alpha-amylase inhibitory activities. In vitro inhibitory activities of these enzymes provide a strong biochemical rationale for further in vivo studies and dietary management strategy for type 2 diabetes through the control of glucose absorption. Further this phenolic antioxidant-enriched dietary strategy using specific beverage combinations can generate a whole food profile that has the potential to reduce hyperglycemia-induced pathogenesis and also associated complications linked to cellular oxidation stress. [source]

In Vitro,Potential of,Ascophyllum nodosum,Phenolic Antioxidant-Mediated ,-Glucosidase and ,-Amylase Inhibition

JOURNAL OF FOOD SCIENCE, Issue 3 2010
E. Apostolidis
ABSTRACT:,Ascophyllum nodosum,is a brown seaweed that grows abundantly in the Northeast coastal region. In this study, the potential of,A. nodosum,for type 2 diabetes management through antioxidant-mediated ,-glucosidase and ,-amylase inhibition was investigated. After the initial screening of 4 locally harvested seaweeds,,A. nodosum,was chosen for its highest phenolic content and was subjected to water extraction. Among extraction ratios of 50 g to 100 to 1000 mL at room temperature, 50 g/400 mL yielded the highest phenolic content of 4.5 mg/g wet weight. For evaluation of extraction temperature ranging from 20 to 80 °C, 50 g/400 mL was chosen as a minimum amount of extractant. Among temperatures studied, extraction at 80 °C resulted in the highest total phenolic contents (4.2 mg/g wet weight). All extracts had similar levels of antioxidant activity in the range of 60% to 70% in terms of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The 80 °C extract had the highest ,-glucosidase and ,-amylase inhibitory activity with IC50 of 0.24 and 1.34 ,g phenolics, respectively, compared to the IC50 of acarbose, reference inhibitor, being 0.37 and 0.68 ,g. The results show that fresh,A. nodosum,has strong ,-glucosidase and mild ,-amylase inhibitory activities that correlated with phenolic contents. This study suggests a nutraceutical potential of,A. nodosum,based on phytochemical antioxidant and antihyperglycemia activities. [source]

Inhibition of , -glucosidase and amylase by bartogenic acid isolated from Barringtonia racemosa Roxb. seeds

PHYTOTHERAPY RESEARCH, Issue 8 2007
P. Mangala Gowri
Abstract Barringtonia racemosa presents a wide range of therapeutic applications. In the course of identifying bioactives from Indian medicinal plants it was observed that the hexane, ethanol and methanol extracts of B. racemosa seeds displayed potent yeast and intestinal , -glucosidase inhibitory activities. The methanol extract was found to be superior among them. However, none of the extracts exhibited pancreatic , -amylase inhibitory activity, rather the ethanol and methanol extracts accelerated the , -amylase enzyme activity. Interestingly, however, bartogenic acid isolated from the methanol extract inhibited , -amylase also. This is the first report identifying , -glucosidase inhibitory activity in B. racemosa seed extracts and assigning to bartogenic acid an , -glucosidase and amylase inhibitory property. The presence of bartogenic acid in B. racemosa seeds as a major compound is also reported for the first time in this communication. Copyright © 2007 John Wiley & Sons, Ltd. [source]