Home About us Contact
|
Home About us Contact | ||
|
|
||
Harvesting Time (harvesting + time)
Selected AbstractsEffects of Whey Permeate-Based Medium on the Proximate Composition of Lentinus edodes in the Submerged CultureJOURNAL OF FOOD SCIENCE, Issue 6 2006Xiaojun Jeffrey Wu ABSTRACT:, Biomass production, crude water-soluble polysaccharide (WSP), ash content, mineral profile, and crude protein content were determined for Lentinus edodes mycelia grown on whey permeate (WP)-based medium with lactose content of 4.5% or defined synthetic medium, and harvested after 5, 10, 15, or 20 d of fermentation at 25 °C. Harvesting time and the type of media interact to alter the chemical content of mycelia. Mycelia grown in WP had greater (P < 0.05) WSP and ash than mycelia grown in the synthetic media. A maximum production of WSP was obtained on the 10th day (4.1 × 102± 71 mg WSP/g dried mycelia) from mycelia grown on the WP-based media. Mycelia grown on WP harvested on the 20th day had the highest value in ash content (18 ± 3%). Potassium was found to be the main constituent in the ash of mushroom mycelia, which was followed by phosphorus, sodium, calcium, and magnesium. A steady increase of ash content was only noted in mycelia grown on WP. The calcium content of WP-grown mycelia was at least 10 times higher compared to mycelia grown in the control media regardless the harvesting time. Data in this research suggested that WP was more favorable than the synthetic media in the production of WSP, which is traditionally known for their medicinal value in L. edodes. [source] The effect of temperature and daylength on heading in tetraploid cultivars of rhodesgrass (Chloris gayana Kunth)GRASSLAND SCIENCE, Issue 4 2005Isao Tarumoto Abstract The present study was conducted to clarify the effect of temperature and daylength on heading in tetraploid cultivars of rhodesgrass (Chloris gayana Kunth). Five cultivars, Tochiraku-kei (2,), Fords Katambora (2,), Callide (4,), Masaba (4,) and Pokot (4,), were planted on three dates at 1-month intervals (4 May, 4 June and 4 July) in three of the Ministry of Agriculture, Forestry and Fisheries research institutions in Ishigaki (Tropical Agriculture Research Center), Nishigoshi (Kyushu National Agricultural Experiment Station) and Nishinasuno (National Grassland Research Institute) in 1978. Three tetraploid cultivars were planted on three dates at 1-month intervals (4 May, 4 June and 4 July) under four daylength-treatments of natural daylength (ND), 12 h daylength [12 h], ND/12 h and 12 h/ND, and changed daylength at the eighth leaf stage, in Nishinasuno in 1978. Under natural daylength, although heading was not observed for Masaba and Pokot, heading was observed in all plots for Tochiraku-kei, Fords Katambora and Callide, and their days to heading (DH) linearly decreased with increases in daily mean temperature (DMT). The result indicated that, under natural daylength in summer, temperature was a major factor in determining DH in tetraploid Callide, which was the same as in diploid rhodesgrass cultivars. Callide reached heading under all four daylength treatments. However, Masaba and Pokot reached heading only under 12 h and ND/12 h treatments, suggesting that Masaba and Pokot have qualitative sensitivity to photoperiods that operate after the ninth leaf stage. These findings would be useful in tetraploid rhodesgrass for estimating foliage harvesting time in the field and for conducting cross-pollination in a greenhouse during winter. [source] Determination of peripheral blood stem cells by the Sysmex SE-9500INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 4 2001Liming Peng The Sysmex SE-9500 automated haematology analyser provides an estimate of immature cells, referred to as ,haematopoietic progenitor cells' (HPC). The aim of this study was to evaluate the reliability and usefulness of the SE-9500 HPC parameter as compared with the CD34 + cell count and to determine whether the HPC count was of value in predicting the optimal harvesting time for peripheral blood stem cells (PBSC). Studies were performed on 112 samples from 21 patients with haematological malignancies and 13 healthy donors undergoing progenitor cell mobilisation. Coefficients of variation for the HPC count were 30%, 23.8%, 12.4% and 8.3% respectively for samples with low (4 × 106/l), medium (13 × 106/l), high (250 × 106/l) and very high (2413 × 106/l) counts. There was good linearity for HPC measurement in both peripheral blood (PB) and purified CD34 + cell suspensions (r > 0.995), and no detectable carryover was observed. There was an acceptable correlation between HPC and CD34 + cell counts for PB samples (r=0.669) and for CD34 + cell suspensions (r=0.859). Analysis of purified CD34 + cells using the SE-9500 HPC mode revealed that they appear both in the blast cell area and the immature granulocyte area of the analyser cell display. Quantitation of CD34 + cells and HPC during PBSC mobilisation showed good agreement between these parameters with regard to the optimal time for PBSC harvesting. These findings suggest that HPC counting with the Sysmex SE-9500 may be clinically useful for optimising the timing of PBSC collection. [source] Harvesting of the Radial Artery for Coronary Artery Bypass Grafting: Comparison of Ultrasonic Harmonic Scalpel Dissector with the Conventional TechniqueJOURNAL OF CARDIAC SURGERY, Issue 3 2009Hosam F. Fawzy M.D. We started routine use of the ultrasonic dissecting scalpel in harvesting radial arteries aiming to minimize harvesting time, improve graft quality, and reduce wound complications. Methods: Radial artery harvesting technique using harmonic scalpel (HS; 43 patients) was compared with the conventional technique (Hemostatic clips and scissors; 53 patients). To avoid spasm, the radial artery was not skeletonized and papaverine was used to irrigate radial artery routinely in all patients. Results: Compared to the conventional technique, radial artery harvesting using the HS has a significantly shorter harvesting time (25 minutes vs. 50 minutes, p < 0.001) and required a significantly smaller number of hemostatic clips (3 vs. 40, p < 0.001). In situ free blood flow was significantly higher in HS group (80 mL/min vs. 40 mL/min, p < 0.001). There was no forearm wound infection in the HS group. There was no graft failure, reoperation for bleeding, or hand ischemia with the use of either technique. Conclusion: Harvesting the radial artery using the HS is less time consuming and decreased the use of hemostatic clips rather atraumatic with good quality graft. [source] THE COMPOSITION OF TWO SPANISH PEPPER VARIETIES (FRESNO DE LA VEGA AND BENAVENTE-LOS VALLES) IN DIFFERENT RIPENING STAGESJOURNAL OF FOOD QUALITY, Issue 6 2008ANA BERNARDO ABSTRACT The chemical composition of two traditional varieties of Spanish peppers, "Fresno de la Vega" and "Benavente-Los Valles" harvested in the provinces of León and Zamora, respectively, at different ripening stages (green mature, breaker and red) were evaluated. Herein we report the global composition, mineral and trace element contents, and the most relevant physicochemical parameters of each variety of pepper collected in 2 consecutive years. Both pepper varieties showed a similar chemical composition, except in vitamin C content, which resulted to be about 70% higher in Fresno de la Vega peppers. Red ripe fruits were high in total carbohydrates, fructose, glucose, sucrose, fat (64.5, 29.14, 25.57, 2.94, 1.79/100 g dry weight, respectively) and total soluble solids (6.83°Brix). Ascorbic acid content also increased progressively during ripening, reaching a mean value of 1.81 mg/100 g dry weight for Fresno de la Vega peppers. Potassium was the most abundant of the analyzed elements in the all samples, followed by phosphorus, magnesium, calcium and sodium. The order of the levels of the trace elements was iron > manganese > zinc > copper. PRACTICAL APPLICATIONS This report underscores the potential value of two traditional varieties of Spanish peppers and provides information on their composition during ripening to decide the proper harvesting time, which can be useful in the food industry. [source] Effects of Whey Permeate-Based Medium on the Proximate Composition of Lentinus edodes in the Submerged CultureJOURNAL OF FOOD SCIENCE, Issue 6 2006Xiaojun Jeffrey Wu ABSTRACT:, Biomass production, crude water-soluble polysaccharide (WSP), ash content, mineral profile, and crude protein content were determined for Lentinus edodes mycelia grown on whey permeate (WP)-based medium with lactose content of 4.5% or defined synthetic medium, and harvested after 5, 10, 15, or 20 d of fermentation at 25 °C. Harvesting time and the type of media interact to alter the chemical content of mycelia. Mycelia grown in WP had greater (P < 0.05) WSP and ash than mycelia grown in the synthetic media. A maximum production of WSP was obtained on the 10th day (4.1 × 102± 71 mg WSP/g dried mycelia) from mycelia grown on the WP-based media. Mycelia grown on WP harvested on the 20th day had the highest value in ash content (18 ± 3%). Potassium was found to be the main constituent in the ash of mushroom mycelia, which was followed by phosphorus, sodium, calcium, and magnesium. A steady increase of ash content was only noted in mycelia grown on WP. The calcium content of WP-grown mycelia was at least 10 times higher compared to mycelia grown in the control media regardless the harvesting time. Data in this research suggested that WP was more favorable than the synthetic media in the production of WSP, which is traditionally known for their medicinal value in L. edodes. [source] DNA Depletion by Precipitation in the Purification of Cell Culture-Derived Influenza VaccinesCHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 6 2010T. Kröber Abstract A pilot study for the purification of cell culture-derived human influenza virus is presented, which focuses on the early removal of DNA by precipitation. Strains of influenza virus were propagated using Madin Darby canine kidney cells as a host. A harvesting time of about 72 h postinfection was chosen to minimize the level of impurities (host cell DNA and protein). Cell culture supernatant was clarified by centrifugation and the performance of this operation was characterized on the basis of Sigma theory. An average clarification efficiency of 93,% (based on turbidity) and a product yield of 85,% (based on hemagglutination activity) were obtained at a load of 1.6,·,10,8 m s,1. Furthermore, the applicability of Sigma theory for scale-up studies using two different laboratory centrifuges was verified. Selective precipitation of DNA was achieved by the addition of polyethyleneimine (PEI). Full factorial design was applied to optimize selectivity considering pH, ionic strength, and the concentration and molecular weight of PEI. Under optimized conditions, treatment with PEI resulted in a reduction of DNA to 15,% of the initial amount, while 86,% of virions (based on neuraminidase activity) were recovered. The subsequent concentration of virions was realized by tangential-flow ultrafiltration. Recovery based on hemagglutination activity was determined to 63,% on average. Including the previous precipitation step, overall reduction in DNA after tangential-flow ultrafiltration was 500-fold. These results indicate that the suggested unit operations are suited for the early depletion of DNA in cell culture-derived influenza vaccine production. [source] Trends in the start of the wet season over AfricaINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 9 2009D. R. Kniveton Abstract A quarter of a century of daily rainfall data from the Global Telecommunications System are used to define the temporal and spatial variability of the start of the wet season over Africa and surrounding extreme south of Europe and parts of the Middle East. From 1978 to 2002, the start of the wet season arrived later in the year for the majority of the region, as time progressed. In some parts of the continent, there was an annual increase in the start date of up to 4 days per year. On average, the start of the wet season arrived 9,21 days later from 1978 to 2002, depending on the threshold used to define the start of the rains (varying from 10,30 mm over 2 days, with no dry period in the following 10 days). It is noted that the inter-annual variability of the start of the wet season is high with the range of start dates varying on average from 116 to 142 days dependent on the threshold used to determine the start date. These results may have important implications for agriculturists on all levels (from the individual farmer to those responsible for regional food supply), as knowledge of potential future climate changes starts to play an increasingly important role in the agricultural decision-making process, such as sowing and harvesting times. Copyright © 2008 Royal Meteorological Society [source] | ||||||||||||||||