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HPTLC Method (hptlc + method)
Selected AbstractsEstimation of Individual Sennosides in Plant Materials and Marketed Formulations by an HPTLC MethodJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 4 2000SHAILESH A. SHAH Senna is a well-known drug, used in the Ayurvedic and Allopathic systems of medicine, and is a treatment for constipation. The purgative action of senna and its formulations is due to the presence of sennosides A and B. An HPTLC method has been developed for the determination of individual sennosides (A, B, C, D) without any derivatization in marketed formulations (three tablet formulations, two granule formulations and one liquid formulation) and plant materials (senna leaf and pod). The methanolic solution of a sample was applied on a pre-coated silica gel G60 F254 TLC plate (E. Merck.) and was developed using n-propanol: ethyl acetate: water: glacial acetic acid (3:3:2:0.1 v/v) as the mobile phase. The relative band speeds (Rf values) obtained were 0.35, 0.25, 0.61, 0.46 for sennosides A, B, C and D, respectively. The densitometric response was monitored at 366 nm. Calibration curves were found to be linear in the concentration ranges 193,1356, 402,2817, 71,497 and 132,927 ng per spot for sennosides A, B, C, and D, respectively. The correlation coefficients were found to be 0.9978, 0.9987, 0.9939 and 0.9983 respectively for sennosides A, B, C and D. The result obtained with the HPTLC method for total sennoside content was compared with the results using the pharmacopoeial methods (spectrophotometric (British Pharmacopoeia) and spectrofluorimetric (United States Pharmacopeia) using the ,F' test). The results revealed no significant difference in the three different methods for estimation of total sennoside. The proposed HPTLC method was found to be simple, specific, precise, accurate and rapid. It can be used for routine quality control of sennosides or senna-containing formulations for individual sennosides. [source] A rapid densitometric method for simultaneous quantification of gallic acid and ellagic acid in herbal raw materials using HPTLCJOURNAL OF SEPARATION SCIENCE, JSS, Issue 6 2005Milind Bagul Abstract Gallic acid and ellagic acid are two widely occurring phenolic compounds of plant origin, to which many biological activities including anticancer and antiviral activity have been attributed. A simple HPTLC method has been developed for the simultaneous quantification of gallic acid and ellagic acid. The method was validated for precision, repeatability, and accuracy. Instrumental precision was found to be 0.083 and 0.78, and the repeatability of the method was found to be 1.07 and 1.50 (% CV) for gallic acid and ellagic acid, respectively. The accuracy of the method was checked by a recovery study conducted at two different levels and the average percentage recovery was found to be 101.02% for gallic acid and 102.42% for ellagic acid. The above method was used for the quantification of gallic acid and ellagic acid content in seeds of Abrus precatorius Linn., whole plant of Phyllanthus maderaspatensis Linn., and flowers of Nymphaea alba Linn. The proposed HPTLC method for the simultaneous quantification of gallic acid and ellagic acid was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of herbal raw materials and for the quantification of these compounds in plant materials. [source] Densitometric determination of zinc bacitracin and nystatin in animal feedJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 9 2008Rade Injac Abstract BACKGROUND: The European Union has forbidden the use of antibiotics as additives in animal feed. Zn-bacitracin (Zn-BC) and nystatin (NYS) were frequently used for their growth-promoting effects and for feed conversion in poultry, pigs and cattle. An HPTLC method has been developed for separating Zn-BC and NYS in the mixture, for routine quality control. RESULTS: The separation was obtained using RP-18 F254S coated HPTLC plates with acetonitrile/methanol (equal volumes):toluene:KH2PO4/KOH (buffer, pH 6.8) = 57:3:40 (v/v/v), adjusted with HCl to pH 8.2, as a mobile phase. The densitograms were monitored at 192, 215 and 305 nm and both antibiotics were assayed at 215 nm. The method was shown to be specific, accurate (recoveries were 98.7 ± 0.5% and 104.8 ± 0.7% for Zn-BC and NYS, respectively), linear over the tested range (correlation coefficients, 0.9982 and 0.9884), and precise (intermediate precision RSD below 2.2% for both analytes) with efficient separation (Rs = 3.5). CONCLUSION: The method was applied for determining Zn-BC and NYS as additives in spiked matrices of commercial animal feedstuffs. According to LOD values for each antibiotic, the minimum detectable amount in feed is 4.5 and 5.5 ppm of Zn-BC and NYS, respectively. Copyright © 2008 Society of Chemical Industry [source] | ||||||||||