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H. Pylori Activity (h + pylori_activity)
Selected AbstractsINHIBITION OF HELICOBACTER PYLORI BY PHENOLIC EXTRACTS OF SPROUTED PEAS (PISUM SATIVUM L.)JOURNAL OF FOOD BIOCHEMISTRY, Issue 1 2006CHIA-YU HO ABSTRACT Infection by Helicobacter pylori is associated with gastric and duodenal ulcers. Conventional treatments to eradicate it have side-effects such as diarrhea and dizziness. The excessive use of antibiotics could also lead to antibiotic-resistant bacteria. The use of plant phenolic phytochemicals can be an alternative because of their health benefits due to both antioxidant activity and antimicrobial activity. The pea (Pisum sativum), the world's second most important pulse crop, produces phenolic phytochemicals with antimicrobial potential. Because the synthesis of phenolic compounds increases with stress, we investigated the anti- H. pylori effectiveness of extracts from pea sprouts, germinated in the dark condition following treatment with either distilled water or acetyl salicylic acid. The peas were germinated for 8 days and the sprouted samples were measured for total soluble phenolic content, antioxidant and guaiacol peroxidase activity. Subsequently, the sprout extracts were tested for anti- H. pylori activity using the agar diffusion method and the effective dose was determined based on phenolic content. The results showed that both acetyl salicylic acid-treated and untreated pea sprouts at days 5 and 8 had anti- H. pylori activity. The minimum volume for inhibition was 50 ,L of extracts. The inhibitory effects were dose dependent. From this study, the potential to use natural phenolic phytochemicals from pea sprouts to control H. pylori was found to be promising. This provides a strategy and foundation to design legume phenolics as functional ingredients against H. pylori. [source] Efficacy of sucralfate for Helicobacter pylori eradication triple therapy in comparison with a lansoprazole-based regimenALIMENTARY PHARMACOLOGY & THERAPEUTICS, Issue 7 2000Adachi Background: Sucralfate has an inhibitory action against Helicobacter pylori and enhances the anti- H. pylori activity of antimicrobials. Aim: To evaluate the efficacy and safety of sucralfate-based eradication therapy for H. pylori infection, compared with that based on lansoprazole, in a randomized multicentre study. Subjects and methods: The subjects were 150 H. pylori -positive patients. They were randomly assigned to one of two regimens for 2 weeks: sucralfate 1 g t.d.s., amoxycillin 500 mg t.d.s., and clarithromycin 400 mg b.d. (SAC regimen: 75 patients); or lansoprazole 30 mg o.m. with the same antimicrobial medications (LAC regimen: 75 patients). Cure of infection was assessed by a 13C urea breath test 1 month after completion of treatment. Results: Eight patients (four in the SAC group and four in LAC group) could not continue therapy because of severe diarrhoea, and three did not take the 13C urea breath test after therapy. Cure rates for intention-to-treat, all-patients-treated, and per protocol analysis in the SAC group were 80%, 83%, and 88%, respectively, and those in the LAC group were 87%, 87%, and 92%, respectively. There were no significant differences in cure rate or adverse effects between the two regimens. Conclusion: Sucralfate in combination with amoxycillin and clarithromycin is as effective as lansoprazole-based eradication therapy for H. pylori. [source] Anti- Helicobacter pylori activity of Chinese tea: in vitro studyALIMENTARY PHARMACOLOGY & THERAPEUTICS, Issue 5 2000Yuk-Kei Yee Background: Chinese tea has an antibacterial activity against a wide range of bacteria. However, its activity against Helicobacter pylori has not been reported. Method: In this study the anti- Helicobacter pylori effects of a Chinese tea (Lung Chen tea), and two tea catechins, epigallocatechin gallate and epicatechin and their minimum inhibitory concentrations (MICs) were examined. The effect of Lung Chen on metronidazole resistance was also studied using the E -test. Results: Lung Chen, epigallocatechin gallate and epicatechin all inhibited the growth of H. pylori. The MIC90 for Lung Chen was 0.25,0.5% (w/w) and that of epigallocatechin gallate and epicatechin were 50,100 and 800,1600 ,g/mL, respectively. Epigallocatechin gallate is probably the active ingredient responsible for most of the anti- H. pylori activity of Chinese tea. Lung Chen did not reverse metronidazole resistance. Conclusions: Chinese tea has anti- H. pylori activity in a daily consumed concentration, and epigallocatechin gallate is probably the active ingredient responsible for the action. [source] Carotenoids with anti- Helicobacter pylori activity from Golden delicious applePHYTOTHERAPY RESEARCH, Issue 5 2010Péter Molnár Abstract Previously it was reported that hypophasic carotenoids of Golden delicious apple peel showed potent anti- H. pylori activity (MIC50 = 36 µg/mL), comparable to metronidazole (MIC50 = 45 µg/mL). To further investigate the involved active carotenoids of the apple peel extracts, seven carotenoids were isolated for the current study: (all- E)-luteoxanthin, (all- E)-neoxanthin, (9,Z)-neoxanthin, (all- E)-antheraxanthin, (all- E)-violaxanthin, (9Z)-violaxanthin and (all- E)-lutein. The MIC50 values of (all- E)-luteoxanthin, (all- E)-neoxanthin and (9,Z)-neoxanthin were 7.9, 11 and 27 µg/mL, respectively. Other carotenoids and ,,,-carotene did not exhibit potent anti- H. pylori activity (MIC50 > 100 µg/mL). An examination of structure and function suggested that active carotenoids contained a monofuranoid ring or an allenic bond in addition to an epoxy group and an additional two or three hydroxyl substituents on the side group. Copyright © 2009 John Wiley & Sons, Ltd. [source] | ||||||||||