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Group P2 (group + p2)

Distribution by Scientific Domains
Chemistry80%

Kinds of Group P2

  • space group p2
    		•

    Selected Abstracts

    Propofol has anti-inflammatory effects on alveolar type II epithelial cells

    ACTA ANAESTHESIOLOGICA SCANDINAVICA, Issue 3 2010
    L. MA
    Background: We investigated whether lipopolysaccharide (LPS) induced inflammation in alveolar epithelial type II (ATII) cells is through cluster of differentiation 14 (CD14) and Toll-like receptor 4 (TLR4) and the effect of different dosages of propofol on the inflammation in primary cultured rat ATII cells. Methods: Cultured ATII cells were randomly assigned to one of the following five groups: Group C: untreated group (control) cultured in the absence of propofol and LPS; Group LPS: treated with 1 ,g/ml LPS; Group P1: treated with 1 ,g/ml LPS and 25 ,M propofol; Group P2: treated with 1 ,g/ml LPS and 50 ,M propofol; Group P3: treated with 1 ,g/ml LPS and 100 ,M propofol. ATII cells in all groups were cultured at 37 °C for 3 h. CD14 and TLR4 mRNA was detected using real-time polymerase chain reaction. Western blot was used to detect CD14 and TLR4 protein expression. CD14 and TLR4 expression on the ATII cells was imaged using immunofluorescence. Tumor necrosis factor-, (TNF-,) production was determined using an ELISA kit. Results: LPS stimulation resulted in an increased CD14 and TLR4 expression and increased TNF-, production in ATII cells. Propofol, at concentrations ,50 ,M, significantly (P<0.05) and dose-dependently decreased CD14 and TLR4 mRNA expression and protein expression in ATII cells. This was accompanied by a decrease in TNF-, production (P<0.05). Conclusion: These results suggest that propofol, at clinically relevant concentrations, can reduce inflammatory responses in LPS-induced ATII cells injury through downregulation of CD14 and TLR4 expression. [source]

    Growth and characterisation of potassium cobalt nickel sulfate hexahydrate for UV light filters

    CRYSTAL RESEARCH AND TECHNOLOGY, Issue 10 2006
    Xinxin Zhuang
    Abstract A new single crystal for ultraviolet light filter, KCNSH (Potassium Cobalt Nickel Sulfate Hexahydrate) was designed and its crystal structure was studied using X-ray diffraction in this paper. The empirical of the title compound is K2Co0.1Ni0.9(SO4)2.6H2O with formula weight 437.15. KCNSH crystal belongs to the monoclinic space group P2(1)/c, a=6.1390(3)Å, b=12.1839(6)Å, c=9.0095(4)Å, ,=,=90°, ,=105.060(2)°, V=650.74(5)Å3, Z=2, Dc=2.231g/cm3. Using the cooling solution method, we have grown a deep green KCNSH crystal with dimension of 12×12×40mm3. The transmission spectrum of KCNSH in the range from UV to near IR wavelengths, its thermal properties, and the relationship between the structure and optical transmission properties are also studied and further discussed in this paper. (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]

    Synthesis and crystal structure of 5-pyrazol-4,5-dihydropyrazoles derivatives

    JOURNAL OF HETEROCYCLIC CHEMISTRY, Issue 5 2008
    Zhengfeng Xie
    A series of 1-acetyl(or phenyl)-3-aryl-5-(1-phenyl-3-methyl-5-aryloxyl-pyrazol)-4,5-dihydropyrazole derivatives have been efficiently synthesized under refluxing in glacial acetic acid with two kinds of hydrazines and five kinds of chalcones of 1-phenyl-methyl-5-phenoxyl-pyrazol-4-aldehyde. The structures were confirmed on the basis of 1H NMR, IR, MS and element analysis, and the crystal structure of the compound 4c was determined by single crystal X-ray diffraction. The compound 4c belongs to monoclinic system with space group P2(1)/n, a = 11.8779(3) nm, b = 12.0901(2) nm, c = 17.7004(4) nm, , = 90°, , = 100.05(10)°, , = 90°, Formula weight: 549.46, Triclinic V = 2502.89(9) nm3, Dc = 1.458 Mg/m3, Z = 4, F (000) = 1128. [source]

    Crystallization and preliminary crystallographic data of a leucotoxin S component from Staphylococcus aureus

    ACTA CRYSTALLOGRAPHICA SECTION D, Issue 2 2004
    Valérie Guillet
    Class S proteins of staphylococcal bicomponent pore-forming leucotoxins play an important role in membrane targetting and cell specificity. Wild-type and recombinant S components of the Panton,Valentine leucocidin (LukS-PV) were expressed in Staphylococcus aureus and Escherichia coli, respectively, and purified. Both proteins were crystallized in two crystal forms with Jeffamine M-­600 as the precipitant at 285,K using the hanging-drop vapour-diffusion method and seeding techniques. Crystals belong to space group P2 (or P21) and P41 (or P43), with unit-cell parameters a = 72.3, b = 95.1, c = 108.1,Å, , = 106.4° and a = b = 94.8, c = 306.2,Å, respectively. A full set of X-ray diffraction data was collected to 2.1,Å from a single tetragonal crystal of the wild-type protein at 100,K. [source]

    Isolation, purification and preliminary X-ray characterization of Cpn60-2 (65,kDa heat-shock protein) from Mycobacterium tuberculosis

    ACTA CRYSTALLOGRAPHICA SECTION D, Issue 9 2002
    Noam Adir
    Cpn60-2 is a member of a unique family of putative molecular chaperones homologous to GroEL (Cpn60) but of unknown function and found only in Mycobacterium tuberculosis and closely related species. Cpn60-2 has mainly been studied for its strong immunogenity. Here, the purification, crystallization and preliminary crystallographic analysis of M. tuberculosis Cpn60-2 are reported. The crystals belong to space group P2, with unit-cell parameters a = 57, b = 115.5, c = 81.5,Å, , = 95.5°, and contain a dimer in the asymmetric unit. The crystals diffract to 4.0,Å using a Cu rotating-anode X-ray generator. [source]

    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2

    ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 8 2010
    Xia Cheng
    Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8,Å resolution and belonged to space group P2, with unit-cell parameters a = 70.2, b = 52.2, c = 153.9,Å, , = 102.8°. [source]

    Crystallization and preliminary crystallographic studies of CorC, a magnesium-ion transporter

    ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 6 2010
    Ning Zhang
    CorC is a magnesium transporter that is involved in the Mg2+ -efflux function of the CorA transporter system, an Mg2+ channel, from Shigella flexneri. Native CorC was purified and crystallized in the native form and in a ligand-free form and diffraction data sets were collected to 2.9 and 3.4,Å resolution, respectively. The native CorC crystals belonged to space group P212121, with unit-cell parameters a = 64.31, b = 74.44, c = 132.78,Å. The ligand-free CorC crystals belonged to space group P3121/P3221, with unit-cell parameters a = b = 71.89, c = 125.96,Å. The CorC,ATP complex has also been crystallized and the crystals belonged to space group P2 or P21. [source]

    Crystallization of dihydrodipicolinate synthase from a clinical isolate of Streptococcus pneumoniae

    ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 1 2010
    Natalia E. Sibarani
    Dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) catalyzes the rate-limiting step in the (S)-lysine biosynthesis pathway of bacteria and plants. Here, the cloning of the DHDPS gene from a clinical isolate of Streptococcus pneumoniae (OXC141 strain) and the strategy used to express, purify and crystallize the recombinant enzyme are described. Diffracting crystals were grown in high-molecular-weight PEG precipitants using the hanging-drop vapour-diffusion method. The best crystal, from which data were collected, diffracted to beyond 2.0,Å resolution. Initially, the crystals were thought to belong to space group P42212, with unit-cell parameters a = 105.5, b = 105.5, c = 62.4,Å. However, the R factors remained high following initial processing of the data. It was subsequently shown that the data set was twinned and it was thus reprocessed in space group P2, resulting in a significant reduction in the R factors. Determination of the structure will provide insight into the design of novel antimicrobial agents targeting this important enzyme from S. pneumoniae. [source]

    Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus

    ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 6 2009
    Alessia Ruggiero
    The thioredoxin system of the archaeon Sulfolobus solfataricus involves a number of different proteins: two thioredoxin reductases (SsTrxRB2 and SsTrxRB3), two distinct thioredoxins (SsTrxA1 and SsTrxA2) and a disulfide oxidoreductase (SsPDO). Here, the crystallization and preliminary crystallographic analyses of SsTrxA1 and SsTrxA2, two dimeric proteins endowed with extraordinary thermal stability, are reported. In addition to the functional thioredoxin domain, both SsTrxA1 and SsTrxA2 present an extra N-terminal fragment of approximately 30 residues. Although crystallization trials have been conducted on both forms of the proteins, crystals that were suitable for X-ray crystallographic analyses have only been obtained for their truncated variants. The crystals of SsTrxA2 belonged to space group P2, with unit-cell parameters a = 28.27, b = 27.88, c = 62.06,Å, , = 92.34°, and diffracted to 1.83,Å resolution, whereas the crystals of SsTrxA1 belonged to space group P21, with unit-cell parameters a = 51.76, b = 75.09, c = 55.35,Å, , = 112.64°, and diffracted to 1.90,Å resolution. The structures of the two proteins have been solved by molecular replacement. [source]

    Preparation, Structural Characterization and Luminescent Property of Binuclear Silver (I) Complex Formed by Benzotriazole and 1-Hydroxymethyl Benzotriazole

    CHINESE JOURNAL OF CHEMISTRY, Issue 9 2002
    Qing-Xiang Liu
    Abstract Dinuclear silver (I) six-membered ring complex [Ag2 (bta)2 -(hmbta)2] (ClO4)2 (3) has been synthesized by the reaction of benzotriazole (bta) (1) and 1-hydroxymethyl benzotriazole (hmbta) (2) with Ag (CH3CN)4ClO4. The structures of compound 2 and Complex 3 have been studied by single crystal X-ray diffraction analysis. The change of luminescent intensity of 1, 2 and 3 was reported. Compound 2 crystallizes in the monoclinic system with space group P2 (1)/c, a = 0.7655 (10) nm, b = 1.0126 (14) nm, c =0.9502 (13) nm, , = 95.07 (2)°, V = 0.7337 (17) nm3 and Z = 4. Complex 3 crystallizes in the triclinic system with space group P1, a = 0.73611 (18) nm, b = 0.9152 (2) nm, c = 1.2277 (3) nm, , = 87.170 (5)°, V = 0.8221 (3) nm3 and Z = 1. The main structural feature of complex 3 is a symmetric dinuclear six-membered ring formed by two silver (I) atoms and four N-atoms from two benzotriazoles. The second structural feature of complex 3 is the ,-, stacking interaction between two adjacent molecular planes, which forms the two-dimentional layer structure. Besides, compared with 2, the luminescent intensity of complex 3 shows a remarkable enhancement. [source]