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Selected AbstractsInterpreting DNA Evidence: A ReviewINTERNATIONAL STATISTICAL REVIEW, Issue 3 2003L.A. Foreman Summary The paper provides a review of current issues relating to the use of DNA profiling in forensic science. A short historical section gives the main statistical milestones that occurred during a rapid development of DNA technology and operational uses. Greater detail is then provided for interpretation issues involving STR DNA profiles, including: , methods that take account of population substructure in DNA calculations; , parallel work carried out by the US National Research Council; , the move away from multiple independence testing in favour of experiments that demonstrate the robustness of casework procedures; , the questionable practice of source attribution ,with reasonable scientific certainty'; , the effect on the interpretation of profiles obtained under increasingly sensitive techniques, the LCN technique in particular; , the use of DNA profiles as an intelligence tool; , the interpretation of DNA mixtures. Experience of presenting DNA evidence within UK courts is also discussed. The paper then summarises a generic interpretation framework based on the concept of likelihood ratio within a hierarchy of propositions. Finally the use of Bayesian networks to interpret DNA evidence is reviewed. Résumé Cet article présente un inventaire des questions relativesá l'utilisation du profilage ADN dans la science légale. Une courte section historique décrit les principales étapes statistiques qui ont eu lieu pendant le rapide développement de la technologie ADN et ses utilisations opérationnelles. De plus grands détails sont ensuite donnés pour l'interprétation de questions sur les profils AND STR, ce qui inclut: ,les méthodes qui tiennent compte des sous-structures de population dans les calculs ADN; ,le travail conduit en paralléle par le Conseil de Recherche Nationale des Etats-Unis (NRC); ,l'évolution depuis les tests d'indépendance multiple vers des expériences qui démontrent la robustesse des procédures; ,la pratique contestable de l'attribution de source avec "certitude scientifique raisonnable"; ,l'effet de l'interprétation des profils obtenus sous techniques de plus en plus sensibles, la technique LCN en particulier ,l'utilisation de profils ADN comme outil d'intelligence; ,l'interprétation de mélanges ADN. L'expérience de ce type de preuve dans les tribunaux britanniques sera également présentée et commentée. L'article présentera un cavenas d'interprétation centré sur le concept de rapport de vraisemblance, inscrit dans une hérarchie de propositions. Finalement, l'utilisation de réseaux Bayesien pour interpréter la preuve par ADN sera abordée. [source] Growth of tin oxide nanocrystalsCRYSTAL RESEARCH AND TECHNOLOGY, Issue 10-11 2005L. Zanotti Abstract Functional oxides are presently much investigated as regards not only their basic properties, but also their actual and potential applications. Tin oxides appear particularly interesting when grown in nanowire shape (SnO2 -NW) because of their electronic- and sensor-device applications. In this paper the results of a systematic study on nucleation and growth of SnO2 -NWs, when an open-tube vapor-transport technique is employed, are reported and discussed. The vapor transport conditions have been investigated in greater detail and for the first time a growth model is proposed. Functional tests at different temperatures and gaseous compositions have proved that the SnO2 -NW are suitable for the preparation of highly-sensitive and stable gas sensors. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Sequential activation of transcription factors in lens inductionDEVELOPMENT GROWTH & DIFFERENTIATION, Issue 5 2000Hajime Ogino Since the pioneering work of the early 1900s, the lens has been used as a model system for the study of tissue development in vertebrates. A number of embryological transplantation experiments designed to elucidate the role of tissue interactions in the formation of the lens have led to the proposal of a stepwise determination model. This model has recently been refined through the identification of certain transcription factor genes, which exhibit distinct expression patterns and functional properties in the lens cell lineage. Otx2, Pax6, and Lens1 are induced by the adjacent anterior neural plate and expressed in predifferentiated lens ectoderm. Contact between the optic vesicle and lens ectoderm promotes expression of mafs, Soxs, and Prox1, which are responsible for the initiation of lens differentiation programs including crystallin expression, cell elongation, and cell cycle arrest. Further analysis of the expression and functional characteristics of these transcription factors will allow greater detail when describing the orchestration of genetic programs, which control tissue development from induction to maturation. [source] Development of cortical and subcortical brain structures in childhood and adolescence: a structural MRI studyDEVELOPMENTAL MEDICINE & CHILD NEUROLOGY, Issue 1 2002Elizabeth R Sowell PhD The purpose of the present study was to describe in greater anatomical detail the changes in brain structure that occur during maturation between childhood and adolescence. High-resolution MRI, tissue classification, and anatomical segmentation of cortical and subcortical regions were used in a sample of 35 normally developing children and adolescents between 7 and 16 years of age (mean age 11 years; 20 males, 15 females). Each cortical and subcortical measure was examined for age and sex effects on raw volumes and on the measures as proportions of total supratentorial cranial volume. Results indicate age-related increases in total supratentorial cranial volume and raw and proportional increases in total cerebral white matter. Gray-matter volume reductions were only observed once variance in total brain size was proportionally controlled. The change in total cerebral white-matter proportion was significantly greater than the change in total cerebral gray-matter proportion over this age range, suggesting that the relative gray-matter reduction is probably due to significant increases in white matter. Total raw cerebral CSF volume increases were also observed. Within the cerebrum, regional patterns varied depending on the tissue (or CSF) assessed. Only frontal and parietal cortices showed changes in gray matter, white matter, and CSF measures. Once the approximately 7% larger brain volume in males was controlled, only mesial temporal cortex, caudate, thalamus, and basomesial diencephalic structures showed sex effects with the females having greater relative volumes in these regions than the males. Overall, these results are consistent with earlier reports and describe in greater detail the regional pattern of age-related differences in gray and white matter in normally developing children and adolescents. [source] Inflammation and the etiology of type 2 diabetesDIABETES/METABOLISM: RESEARCH AND REVIEWS, Issue 1 2006Åke Sjöholm Type 2 diabetes is increasingly common worldwide and is beginning to strike younger age groups. Almost 90% of all patients with diabetes show insulin resistance, which also precedes the first symptoms of diabetes. The mechanisms underlying the development of insulin resistance are not well understood. In recent years, several studies have been published that implicate subclinical chronic inflammation as an important pathogenetic factor in the development of insulin resistance and type 2 diabetes. This opens new perspectives for diagnosis and treatment of early insulin resistance and incipient glucose intolerance. Surrogate markers for this low-grade chronic inflammation include CRP, IL-6 and TNF-,. Some antidiabetic agents, for example, glitazones that reduce insulin resistance, and insulin itself, reduce inflammation. Conversely, antiinflammatory drugs (ASA/NSAID) may improve glucose tolerance. Vasoactive drugs that are often prescribed to people with diabetes, for example, statins and ACE inhibitors/angiotensin receptor antagonists, also counteract inflammation and reduce the risk of type 2 diabetes. More specific and sensitive biomarkers should be identified, which may predict early disturbances in insulin sensitivity and cardiovascular risk. Also, inflammatory signalling pathways need to be explored in greater detail, and may form the basis of drugable targets against the epidemic of insulin resistance and atherosclerosis. Copyright © 2005 John Wiley & Sons, Ltd. [source] Enjoying the saints in late antiquityEARLY MEDIEVAL EUROPE, Issue 1 2000Peter Brown The discovery at Mainz by Fran,ois Dolbeau of a new collection of sermons of Augustine has enabled us to study, in far greater detail, the attitude of Augustine to the reform of the cult of the martyrs between 391 and 404. This study aims to understand Augustine's insistence on the need to imitate the martyrs against the background of his views on grace and the relation of such views to the growing differentiation of the Christian community. It also attempts to do justice to the views of those he criticized: others regarded the triumph of the martyrs over pain and death as a unique manifestation of the power of God, in which believers participated, not through imitation but through celebrations reminiscent of the joy of pagan festivals. In this debate, Augustine by no means had the last word. The article attempts to show the continuing tension between notions of the saints as imitable and inimitable figures in the early medieval period, and more briefly, by implication, in all later centuries. [source] Erythropoiesis and red cell function in vertebrate embryosEUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 2005R. Baumann Abstract All vertebrate embryos produce a specific erythroid cell population , primitive erythrocytes , early in development. These cells are characterized by expression of the specific embryonic haemoglobins. Many aspects of primitive erythropoiesis and the physiological function of primitive red cells are still enigmatic. Nevertheless, recent years have seen intensive efforts to characterize in greater detail the molecular events underlying the initiation of erythropoiesis in vertebrate embryos. Several key genes have been identified that are necessary for primitive and the subsequent definitive erythropoiesis, which differs in several aspect from primitive erythropoiesis. This review gives in its first part a short overview dealing with comparative aspects of primitive and early definitive erythropoiesis in higher and lower vertebrates and in the second part we discuss the physiological function of primitive red cells based mainly on results from mammalian and avian embryos. [source] Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis -specific CD4+ memory T lymphocyte populationsEUROPEAN JOURNAL OF IMMUNOLOGY, Issue 11 2007Natalie Abstract In the search for effective vaccines against intracellular pathogens such as HIV, tuberculosis and malaria, recombinant viral vectors are increasingly being used to boost previously primed T cell responses. Published data have shown prime-boost vaccination with BCG-MVA85A (modified vaccinia virus Ankara expressing antigen 85A) to be highly immunogenic in humans as measured by ex vivo IFN-, ELISPOT. Here, we used polychromatic flow cytometry to investigate the phenotypic and functional profile of these vaccine-induced Mycobacterium tuberculosis (M.tb) antigen 85A-specific responses in greater detail. Promisingly, antigen 85A-specific CD4+ T cells were found to be highly polyfunctional, producing IFN-,, TNF-,, IL-2 and MIP-1,. Surface staining showed the responding CD4+ T cells to be relatively immature (CD45RO+ CD27intCD57,); this observation was supported by the robust proliferative responses observed following antigenic stimulation. Furthermore, these phenotypic and functional properties were independent of clonotypic composition and epitope specificity, which was maintained through the different phases of the vaccine-induced immune response. Overall, these data strongly support the use of MVA85A in humans as a boosting agent to expand polyfunctional M.tb -specific CD4+ T cells capable of significant secondary responses. [source] Cellular mechanisms of the trigeminally evoked startle responseEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 7 2003Susanne Schmid Abstract The startle response is an important mammalian model for studying the cellular mechanisms of emotions and of learning. It consists of contractions of facial and skeletal muscles in response to sudden acoustic, tactile or vestibular stimuli. Whereas the acoustic startle pathway is well described, only a few recent studies have investigated the tactile startle pathway. It was proposed that there is a direct projection from the principal sensory nucleus to the central sensorimotor interface of the startle response, which is formed by the giant neurons in the caudal pontine reticular formation. We explored this projection in greater detail in vitro. Anterograde tracing in rat brain slices confirmed projections with large axon terminals from the ventral part of the principal sensory nucleus to the lateral caudal pontine reticular formation. Electrophysiological studies revealed a monosynaptic glutamatergic connection between principal sensory nucleus neurons and caudal pontine reticular formation giant neurons. The synapses displayed paired-pulse facilitation at high-frequency stimulation, and homosynaptic depression at 1 Hz stimulation. The latter form of plasticity is thought to underlie habituation of the startle response. Furthermore, postsynaptic currents in caudal pontine reticular formation giant neurons evoked by principal sensory nucleus neuron stimulation summed in a linear way with signals evoked by stimulation of auditory afferents. Synaptic plasticity and summation of synaptic currents correspond well with in vivo data previously published by other groups. We thus presume that these synapses mediate trigeminal input to the startle pathway. [source] PROGRAMS FOR PROMOTING PARENTING OF RESIDENTIAL PARENTS: Moving From Efficacy to EffectivenessFAMILY COURT REVIEW, Issue 1 2005Sharlene A. Wolchik This article reviews prevention programs that target primary residential parents as change agents for improving children's postdivorce adjustment. First, we review parental risk and protective factors for children from divorced families, including parenting quality, parental mental health problems, interparental conflict, and contact with the nonresidential parent. Following a discussion of brief informational interventions, we describe the findings of evaluations of three multisession, skill-building interventions for divorced parents. Impressive evidence is presented that parenting is a modifiable protective factor and that improving parenting leads to improvements in children's postdivorce adjustment. We then discuss, in greater detail, the New Beginnings Program, which we highlight because it has shown repeated, immediate effects on children's mental health outcomes as well as long-term effects on a wide array of other meaningful outcomes, such as diagnosis of mental disorder in the past year, externalizing problems, alcohol and drug use, and academic performance. Also, mediational analyses have shown that program-induced changes in parenting accounted for changes in mental health outcomes. The remainder of the article describes a research and action agenda that is needed to successfully implement the New Beginnings Program in domestic relations courts. [source] An overview of the damage approach of durability modelling at elevated temperatureFATIGUE & FRACTURE OF ENGINEERING MATERIALS AND STRUCTURES, Issue 6 2001J.-L. Chaboche Lifetime prediction techniques for components working at elevated temperature are revisited. Two damage approaches in which time effects at high temperature are introduced in different ways are discussed in greater detail. First, a creep,fatigue damage model considers the interaction of the two processes during the whole life before macrocrack initiation; and second, a creep,fatigue,oxidation model separates the fatigue life into two periods: during initiation the environment-assisted processes interact with fatigue, although bulk creep damage only interacts during the micropropagation period. The second model is illustrated by its application to a coated single-crystal superalloy used in aerojet turbine blades. Its capabilities are illustrated in a number of isothermal and thermomechanical fatigue tests. Anisotropy effects are also briefly discussed and a special test, introducing cyclic thermal gradients through the wall thickness of a tubular component, demonstrates the predictive capabilities for actual engine conditions. [source] Dengue virus-specific suppressor T cells: current perspectivesFEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 3 2007Umesh C. Chaturvedi Abstract Dengue virus was the first microorganism that was shown to induce generation of antigen-specific suppressor T (TS) cells in mice. The cascade of the three generations of TS cells (TS1, TS2, TS3) and their secretary products, the suppressor factors (SF1, SF2), was delineated. The TS pathway was proposed to be protective through inhibition of the production of enhancing antibody, which may enhance the severity of dengue disease. The currently second most favoured mechanism of severe dengue disease is the ,cytokine tsunami'. During the last decade, suppressor/regulatory T cells have been studied in greater detail using modern techniques in various diseases, including viral infections. This brief review discusses the role of dengue-specific suppressor T cells in protection and/or induction of severe dengue disease in view of our current understanding of suppressor/regulatory T cells. [source] Regional socio-economic importance of fisheries in FinlandFISHERIES MANAGEMENT & ECOLOGY, Issue 4-5 2001J. Virtanen The total value of Finnish fisheries production in 1997 was almost FIM 2 billion. The bulk of this sum was from processing and wholesaling, but in terms of value added, somewhat more than half was contributed by the primary sector, i.e. fishing and fish farming. In economic terms, fisheries accounted for 0.1% of gross domestic product (GDP) in 1997 and the relative job creation capacity was 0.15%. This paper examines the structure of Finnish fisheries in terms of production value and employment. It also considers the level of dependency on fisheries and of value added along the production chain. The focus is on concentration of the industry and on input to the economy at the regional level. The concentration of fisheries and regional dependence on fisheries are assessed in absolute and relative terms. Examining employment and value added in these two ways enables the regional nature of fisheries to be shown in greater detail. The input of fisheries to the regional economy is examined by the share of value added and by location quotients. The location quotient compares an area's share of a particular activity with that area's share of some basic or aggregated phenomenon. The location quotient showed that fisheries have importance not only in the coastal area but also inland. [source] Shear wave velocity model of the Santiago de Chile basin derived from ambient noise measurements: a comparison of proxies for seismic site conditions and amplificationGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 1 2010Marco Pilz SUMMARY We determined a high-resolution 3-D S -wave velocity model for a 26 km × 12 km area in the northern part of the basin of Santiago de Chile. To reach this goal, we used microtremor recordings at 125 sites for deriving the horizontal-to-vertical (H/V) spectral ratios that we inverted to retrieve local S -wave velocity profiles. In the inversion procedure, we used additional geological and geophysical constraints and values of the thickness of the sedimentary cover already determined by gravimetric measurements, which were found to vary substantially over short distances in the investigated area. The resulting model was derived by interpolation with a kriging technique between the single S -wave velocity profiles and shows locally good agreement with the few existing velocity profile data, but allows the entire area, as well as deeper parts of the basin, to be represented in greater detail. The wealth of available data allowed us to check if any correlation between the S -wave velocity in the uppermost 30 m (v30S) and the slope of topography, a new technique recently proposed by Wald and Allen, exists on a local scale. We observed that while one lithology might provide a greater scatter in the velocity values for the investigated area, almost no correlation between topographic gradient and calculated v30S exists, whereas a better link is found between v30S and the local geology. Finally, we compared the v30S distribution with the MSK intensities for the 1985 Valparaiso event, pointing out that high intensities are found where the expected v30S values are low and over a thick sedimentary cover. Although this evidence cannot be generalized for all possible earthquakes, it indicates the influence of site effects modifying the ground motion when earthquakes occur well outside of the Santiago basin. [source] Publicly funded medical savings accounts: expenditure and distributional impacts in Ontario, CanadaHEALTH ECONOMICS, Issue 10 2008Jeremiah Hurley Abstract This paper presents the findings from simulations of the introduction of publicly funded medical savings accounts (MSAs) in the province of Ontario, Canada. The analysis exploits a unique data set linking population-based health survey information with individual-level information on all physician services and hospital services utilization over a four-year period. The analysis provides greater detail along three dimensions than have previous analyses: (1) the distributional impacts of publicly funded MSAs across individuals of differing health statuses, incomes, ages, and current expenditures; (2) the impact of differing degrees of risk adjustment for MSA contributions; and (3) the impact of MSA funding over multiple years, incorporating year-to-year variation in spending at the individual level. In addition, it analyses more plausible designs for publicly funded MSAs than the existing studies. Government uses information available from year t,,,1 to allocate its budget for year t in a manner that is ex ante fiscally neutral for the public sector: the government first withholds funds equal to expected catastrophic insurance payments under the MSA plan, and then allocates only the balance to individual MSA accounts. The government captures the savings associated with reduced health-care utilization under MSAs and we examine deductibles that vary by income rather than by current health-care expenditures. The impacts on public expenditures under these designs are more modest than in the previous studies and under plausible assumptions MSAs are predicted to decrease public expenditures. MSAs, however, are also predicted to have unavoidable negative distributional consequences with respect to both public expenditures and out-of-pocket spending. Copyright © 2007 John Wiley & Sons, Ltd. [source] Hepatic expression of gamma-glutamyltranspeptidase in the human liver of patients with alcoholic liver diseaseHEPATOLOGY RESEARCH, Issue 11 2007Makoto Irie Background:, Gamma-glutamyltranspeptidase (GGT) has been recognized as an enzyme that converts glutathione into cysteine, and it is localized predominantly within the liver. Serum GGT is clinically recognized as the most useful marker for diagnosis of alcoholic liver disease (ALD). Methods:, GGT localization within the liver was examined immunohistochemically using an anti-GGT antibody and was visualized by confocal laser scanning microscopy in ALD and normal livers. Double immunostaining for GGT and dipeptidylpeptidase-IV (DPP-IV) was carried out to evaluate GGT localization in greater detail. Results:, Expression of GGT protein and mRNA was studied with immunoblot analysis and in situ hybridization, respectively. Immunohistochemically, the expression of GGT in the normal liver was faintly demonstrated in the bile canaliculi of hepatocytes and in biliary epithelial cells. In ALD livers, GGT was clearly demonstrated at the same sites. Double immunostaining demonstrated that GGT and DPP-IV were colocalized in hepatocytes in the ALD liver. In situ hybridization clearly demonstrated GGT-mRNA within the cytoplasm of hepatocytes and biliary epithelial cells. Immunoblot analysis revealed that GGT protein expression was increased in the ALD livers compared with that seen in the normal livers. Conclusion:, These findings indicate that GGT in control and alcoholic livers is synthesized in hepatocytes and biliary epithelial cells, and is localized within the bile canalicular membrane and the luminal membrane in those cells, respectively. In conclusion, GGT synthesis and protein expression are increased in ALD livers, leading to the elevation of serum levels of GGT that are commonly noted in patients with the disease. [source] Privatising social housing in TaiwanINTERNATIONAL JOURNAL OF SOCIAL WELFARE, Issue 1 2007William D.H. Li This article first reviews how the concept of privatisation has been referred to in the current restructuring of the social housing system, especially in the case of the UK. The term ,privatisation' is then examined in greater detail and its wider meaning is discussed. By using the network approach, privatisation in relation to housing can be understood in terms of the changing combinations of agents involved in providing social housing, which gives rise to the commodified impact on the distribution of social housing. By using the privatisation process of social housing in Taiwan as an example, three distinct combinations in terms of providing social housing are identified. With an increasing number of social housing units being provided by the marketised social housing model where private agents control the process of providing social housing, along with more market rules being involved in the provision and the partial removal of means tests in relation to the distribution of social housing, the privatisation of social housing development in Taiwan is having a major impact on equity. [source] Exploring the Afromontane centre of endemism: Kniphofia Moench (Asphodelaceae) as a floristic indicatorJOURNAL OF BIOGEOGRAPHY, Issue 12 2008Syd Ramdhani Abstract Aim, The genus Kniphofia contains 71 species with an African,Malagasy distribution, including one species from Yemen. The genus has a general Afromontane distribution. Here we explore whether Kniphofia is a floristic indicator of the Afromontane centre of endemism and diversity. The South Africa Centre of diversity and endemism was explored in greater detail to understand biogeographical patterns. Location, Africa, Afromontane Region, southern Africa, Madagascar and Yemen. Methods, Diversity and endemism for the genus were examined at the continental scale using a chorological approach. Biogeographical patterns and endemism in the South Africa Centre were examined in greater detail using chorology, phenetics, parsimony analysis of endemicity (PAE) and mapping of range-restricted taxa. Results, Six centres of diversity were recovered, five of which are also centres of endemism. Eight subcentres of diversity are proposed, of which only two are considered subcentres of endemism. The South Africa Centre is the most species-rich region and the largest centre of endemism for Kniphofia. The phenetic analysis of the South Africa Centre at the full degree square scale recovered three biogeographical areas that correspond with the subcentres obtained from the chorological analysis. The PAE (at the full degree square scale) and the mapping of range-restricted taxa recovered two and six areas of endemism (AOEs), respectively. These latter two approaches produced results of limited value, possibly as a result of inadequate collecting of Kniphofia species. Only two AOEs were identified by PAE and these are embedded within two of the six AOEs recovered by the mapping of range-restricted taxa. All the above AOEs are within the three subcentres found by chorological and phenetic analysis (at the full degree square scale) for the South Africa Centre. Main conclusions, The centres for Kniphofia broadly correspond to the Afromontane regional mountain systems, but with some notable differences. We regard Kniphofia as a floristic indicator of the Afromontane Region sensu lato. In southern Africa, the phenetic approach at the full-degree scale retrieved areas that correlate well with those obtained by the chorological approach. [source] The flora of the South Sandwich Islands, with particular reference to the influence of geothermal heatingJOURNAL OF BIOGEOGRAPHY, Issue 6 2000P. Convey Abstract Aim, To carry out as comprehensive a survey as practicable of the flora (higher plants, mosses, liverworts, lichens, basidiomycete fungi and diatoms) of the isolated, volcanically active, South Sandwich Islands archipelago in the southern South Atlantic. To relate the components of this flora to (1) the influence of local geothermal heating and (2) wider regional floras. Location, South Sandwich Islands, southern South Atlantic Ocean, maritime Antarctic (56,60° S, 26,28° W). Methods, Ice-free accessible sites on all 11 of the major islands in the archipelago were visited by helicopter in January 1997. During each visit, voucher specimens of each floral group were collected. The comprehensiveness of collections obtained at each site varied with the duration of each visit (a function of tight logistic constraints) and extent of site. Visit duration varied from 1 to 9 h at most sites, with longer periods spent on Bellingshausen Island (2 days) and Leskov Island (1 day). Candlemas Island was examined in greater detail over a 4-week period in February 1997. At all sites, particular attention was given to areas influenced by geothermal heating. Results, Data obtained in 1997 are combined with updated records from the only previous survey (in 1964) to provide a baseline description of the flora of the archipelago, which currently includes 1 phanerogam, 38 mosses, 11 liverworts, 5 basidiomycete fungi, 41 lichenised fungi and 16 diatoms with, additionally, several taxa identified only to genus. Major elements of the moss and liverwort floras are composed of South American taxa (32% and 73%, respectively), with a further 45% of mosses having bipolar or cosmopolitan distributions. These two groups show low levels of Antarctic endemicity (11% and 18%, respectively). In contrast, 52% of lichens and 80% of basidiomycete fungi are endemic to the Antarctic. A further 36% of lichens are bipolar/cosmopolitan, with only 5% of South American origin. Main Conclusions, The flora of the South Sandwich Islands is clearly derived from those of other Antarctic zones. The flora of unheated ground is closely related to that of the maritime Antarctic, although with a very limited number of species represented. That of heated ground contains both maritime and sub-Antarctic elements, confirming the importance of geothermal heating for successful colonisation of the latter group. The occurrence of several maritime Antarctic species only on heated ground confirms the extreme severity of the archipelago's climate in comparison with well-studied sites much further south in this biogeographical zone. [source] DETERMINATION OF VARIABLE DIFFUSION OF SODIUM DURING DEBITTERING OF GREEN OLIVESJOURNAL OF FOOD PROCESS ENGINEERING, Issue 5 2004MARIELA BEATRIZ MALDONADO ABSTRACT Time-variable average effective diffusion coefficients of sodium in the skin and flesh were determined using Macroscopic Mass Balances from experimental data obtained during debittering of green olives, variety Arauco at temperatures of 15, 20 and 25C and lye concentrations of 1.50, 2.25 and 3.00% of NaOH. The effective diffusion coefficient of sodium increased with treatment time at both surfaces of the skin, while it varied very little within the flesh, and in many cases, remained almost constant during the debittering process. In general, the diffusion coefficients determined in the present study were within an order of magnitude of 10,10 m2/s for the flesh during most of the treatment time, while for the skin, they were of the order of 10,12 m2/s for the initial treatment times. Macroscopic mass balances are a simple mathematical method that can be used in lieu of more common numerical techniques, to evaluate variable diffusion coefficients providing accuracy and greater detail of the whole phenomena. [source] The Association Between Partner Enhancement and Self-Enhancement and Relationship Quality OutcomesJOURNAL OF MARRIAGE AND FAMILY, Issue 3 2009Dean M. Busby The purpose of this research was to understand in greater detail, using 2 samples (Study 1 N = 4,881 heterosexual couples; Study 2 N = 335 heterosexual couples who completed the Relationship Evaluation Questionnaire), how partner or self-enhancement patterns differentially influence relationship outcomes. A multivariate analysis of covariance was conducted comparing 4 outcome measures for different couple types in which individuals rated the partner higher, the same, or lower than they rated themselves on affability. Couples in which both individuals perceived themselves as more affable than the partner experienced poorer results on the relationship outcome measures, whereas couples in which both individuals perceived the partner's personality as more affable than their own experienced more positive relationship outcomes. Additional analyses with structural equation models demonstrated the consistent influence of enhancement measures on relationship outcomes for cross-sectional and longitudinal samples. [source] Putative signaling action of amelogenin utilizes the Wnt/,-catenin pathwayJOURNAL OF PERIODONTAL RESEARCH, Issue 3 2009M. Matsuzawa Background and Objective:, While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. Material and Methods:, To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a ,-galactosidase transgene under the control of a LEF/TCF and ,-catenin-inducible promoter. To investigate in greater detail the molecular mechanisms involved in the ,-catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full-length recombinant mouse amelogenin and were evaluated for phenotypic changes and ,-catenin signaling using a TOPFLASH construct and the LacZ reporter gene. Results:, In these in vitro models, we showed that amelogenin can activate ,-catenin signaling. Conclusion:, Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone. [source] Subculture affects the phenotypic expression of human periodontal ligament cells and their response to fibroblast growth factor-2 and bone morphogenetic protein-7,in vitroJOURNAL OF PERIODONTAL RESEARCH, Issue 5 2008S. Lossdörfer Background and Objective:, Although periodontal ligament cells display several osteoblastic traits, their phenotypic expression is still not well established. It remains a matter of debate whether they resemble a terminally differentiated cell type or an intermediate maturation state that potentially can be directed towards a fibroblastic or an osteoblastic phenotype. Material and Methods:, To explore the characteristics of periodontal ligament cells in greater detail, fourth-passage, sixth-passage and eighth-passage human periodontal ligament cells were cultured for up to 3 wk. Ki-67, alkaline phosphatase, osteocalcin, osteoprotegerin and receptor activator of nuclear factor-,B ligand (RANKL) mRNA expression was quantified by real-time polymerase chain reaction. Furthermore, the cellular response to fibroblast growth factor-2 and bone morphogenetic protein-7 was examined in first-passage and fourth-passage cells. Dermal fibroblasts (1BR.3.G) and osteoblast-like cells (MG63) served as reference cell lines. Results:, Proliferation decreased over time and was highest in fourth-passage cells. The expression of differentiation parameters, osteoprotegerin and RANKL increased with culture time and was higher in fourth-passage cells than in cells of later passages. The RANKL/osteoprotegerin ratio increased steadily until day 21. Administration of fibroblast growth factor-2 enhanced cell numbers in both passages, whereas alkaline phosphatase and osteocalcin production remained unchanged. By contrast, exposure of periodontal ligament cells to bone morphogenetic protein-7 resulted in a reduction of cell number in the first and fourth passages, whereas the production of alkaline phosphatase and osteocalcin was enhanced. In dermal fibroblasts, differentiation parameters did not respond to both stimuli. MG63 cells behaved similarly to periodontal ligament cells. Conclusion:, These results indicate that subculture affects the phenotypic expression of human periodontal ligament cells with respect to the characteristics that these cells share with osteoblasts. Furthermore, the periodontal ligament cell phenotype can be altered by fibroblastic and osteoblastic growth factors. [source] Assessing oral cancer early detection: clarifying dentists' practicesJOURNAL OF PUBLIC HEALTH DENTISTRY, Issue 2 2010Charles W. LeHew PhD Abstract Objective: This study explores new methods for assessing in greater detail what dentists do when they perform oral cancer early detection examinations. It clarifies practice behaviors and opens opportunities to identify factors that facilitate thorough early detection examinations by clinicians and to assess the relative effectiveness of different examination procedures. Methods: A 38-item survey instrument was e-mailed to dentists in a western US, multistate dental practice group. Questionnaires were received by 241 dentists, and 102 responded. An Oral Cancer Knowledge scale (0 to 14) was generated from correct responses on oral cancer general knowledge. An Oral Cancer Examination Thoroughness scale was calculated from the two dimensions of reported usage and frequency of procedures in oral cancer examinations. Results: Nearly all responding dentists were in general practice (90%), with a median year of graduation from dental school of 1994. The Oral Cancer Knowledge scores ranged from 5 to 14 with a mean of 10.4. The mean Thoroughness of Examination score was 11.34 (range 0 to 20). The two scales were not statistically correlated (r = ,0.015, P = 0.883). Statistically, recency of continuing education was significantly associated with knowledge (P = 0.0284) and appears to be marginally associated with thoroughness (P = 0.075). Conclusions: This study documents considerable variability in dentists' knowledge and thoroughness of examinations. The scales provide tools for future studies for improving understanding of early detection of oral cancer in clinical practice. [source] SPECIAL ARTICLE: A review of the International Brain Research Foundation novel approach to mild traumatic brain injury presented at the International Conference on Behavioral Health and Traumatic Brain InjuryJOURNAL OF THE AMERICAN ACADEMY OF NURSE PRACTITIONERS, Issue 9 2010MSN (Doctoral Student), Mary Zemyan Polito CRNP Abstract "The International Conference on Behavioral Health and Traumatic Brain Injury" held at St. Joseph's Regional Medical Center in Paterson, NJ., from October 12 to 15, 2008, included a presentation on the novel assessment and treatment approach to mild traumatic brain injury (mTBI) by Philip A. DeFina, PhD, of the International Brain Research Foundation (IBRF). Because of the urgent need to treat a large number of our troops who are diagnosed with mTBI and post-traumatic stress disorder (PTSD), the conference was held to create a report for Congress titled "Recommendations to Improve the Care of Wounded Warriors NOW. March 12, 2009." This article summarizes and adds greater detail to Dr. DeFina's presentation on the current standard and novel ways to approach assessment and treatment of mTBI and PTSD. Pilot data derived from collaborative studies through the IBRF have led to the development of clinical and research protocols utilizing currently accepted, valid, and reliable neuroimaging technologies combined in novel ways to develop "neuromarkers." These neuromarkers are being evaluated in the context of an "Integrity-Deficit Matrix" model to demonstrate their ability to improve diagnostic accuracy, guide treatment programs, and possibly predict outcomes for patients suffering from traumatic brain injury. [source] Eye tracking and online search: Lessons learned and challenges aheadJOURNAL OF THE AMERICAN SOCIETY FOR INFORMATION SCIENCE AND TECHNOLOGY, Issue 7 2008Lori Lorigo This article surveys the use of eye tracking in investigations of online search. Three eye tracking experiments that we undertook are discussed and compared to additional work in this area, revealing recurring behaviors and trends. The first two studies are described in greater detail in Granka, Joachims, & Gay (2004), Lorigo et al. (2006), and Pan et al. (2007), and the third study is described for the first time in this article. These studies reveal how users view the ranked results on a search engine results page (SERP), the relationship between the search result abstracts viewed and those clicked on, and whether gender, search task, or search engine influence these behaviors. In addition, we discuss a key challenge that arose in all three studies that applies to the use of eye tracking in studying online behaviors which is due to the limited support for analyzing scanpaths, or sequences of eye fixations. To meet this challenge, we present a preliminary approach that involves a graphical visualization to compare a path with a group of paths. We conclude by summarizing our findings and discussing future work in further understanding online search behavior with the help of eye tracking. [source] Trawl-induced bottom disturbances off the south coast of Portugal: direct observations by the ,Delta' manned-submersible on the Submarine Canyon of PortimãoMARINE ECOLOGY, Issue 2007Paulo Morais Abstract The effects of marine fishing activities on benthic habitat and communities have become an important environmental issue. In addition to the direct removal of target species, effects include by-catch, damage to benthic organisms and alteration of habitat structure. The growing number of studies on the impact of fishing on bottom habitats indicates that the effects vary with the physical nature of the seabed and with the local natural disturbance regime. Several studies have been conducted on fisheries by-catch and discards off the south coast of Portugal since 1996. The results provide an idea of the impact on biodiversity: more than 60% of the species caught by the trawl fishery are discarded. The crustacean trawl captures the greatest number of species, probably due to the greater fishing depth range. In April 2004, the manned submersible ,Delta' (from DELTA Oceanographics, USA) conducted a sea campaign integrated in the SEMAPP programme (Science, Education, and Marine Archaeology Program in Portugal) to directly observe and assess the biological, geological, and archaeological aspects. A total of 15 dives were conducted mainly near the head and in the flanks inside Portimão canyon, down to 300 m. Observations showed variations in bottom type, the sedimentary framework and biological communities. These dives (150,300 m depth) also revealed a heavily trawled canyon bottom and erosion structures leading to changes in habitat structure and biodiversity. Of special interest was the occurrence of low-relief boulder substrates with relatively high densities of demersal finfish and shellfish. These sites served as a refuge for several species, whose abundance here was greater than on the surrounding areas of fine-grained substrates. Strong marks on the bottom are apparently caused by the doors of the trawl nets. Their impact will be examined in greater detail in future studies. [source] A review of evaluation outcomes of web-based continuing medical educationMEDICAL EDUCATION, Issue 6 2005Vernon R Curran Introduction, The Internet and worldwide web have expanded opportunities for the provision of a flexible, convenient and interactive form of continuing medical education (CME). Larger numbers of doctors are accessing and using the Internet to locate and seek medical information. It has been suggested that a significant proportion of this usage is directly related to questions that arise from patient care. A variety of Internet technologies are being used to provide both asynchronous and synchronous forms of web-based CME. Various models for designing and facilitating web-based CME learning have also been reported. The purpose of this study was to examine the nature and characteristics of the web-based CME evaluative outcomes reported in the peer-reviewed literature. Methods, A search of Medline was undertaken and the level of evaluative outcomes reported was categorised using Kirkpatrick's model for levels of summative evaluation. Results, The results of this analysis revealed that the majority of evaluative research on web-based CME is based on participant satisfaction data. There was limited research demonstrating performance change in clinical practices and there were no studies reported in the literature that demonstrated that web-based CME was effective in influencing patient or health outcomes. Discussion, The findings suggest an important need to examine in greater detail the nature and characteristics of those web-based learning technologies, environments and systems which are most effective in enhancing practice change and ultimately impacting patient and health outcomes. This is particularly important as the Internet grows in popularity as a medium for knowledge transfer. [source] Antigenic cross-reactivity between different alleles of the Plasmodium falciparum merozoite surface protein 2PARASITE IMMUNOLOGY, Issue 11-12 2003Ingrid Felger SUMMARY The polymorphic domain of the gene encoding Plasmodium falciparum merozoite surface protein 2 (MSP2) was PCR amplified from blood of malaria patients, genotyped, and 19 distinct fragments were cloned and expressed in E. coli. The reactivity of naturally occurring antibodies against this panel of recombinant MSP2 antigens was tested using 67 homologous or heterologous sera from a serum bank of travel clinic patients. Sera from semi-immune individuals strongly recognized almost all recombinant antigens. Sera from primary infected patients either did not react at all (9 sera), or reacted weakly against varying numbers of antigens (39 sera). The antigens that showed reactions were mostly of the allelic family corresponding to the infecting clone, but in very few cases also of the alternative allelic family. Single clone infections and repeated samples from the same individual were analysed in greater detail. Thus, we were able to quantify cross-reactivity induced by a single P. falciparum infection. Within the two allelic families of MSP2, cross-reactivity was observed between some but not all alleles of the same family, whereas antibodies cross-reactive between variants belonging to different allelic families were detected in only a few cases. [source] Flavin-sensitized Photo-oxidation of Lysozyme and Serum AlbuminPHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 4 2009Yazhou Zhang The excited state processes of riboflavin, flavin mononucleotide and flavin adenine dinucleotide in argon-saturated aqueous solution were studied in the presence of lysozyme or bovine serum albumin (BSA). UV,Vis absorption and fluorescence spectroscopy indicates that the noncovalent flavin-protein binding is relatively weak. Quenching of the flavin triplet state by BSA, observed by time-resolved photolysis, is less efficient than by lysozyme. Light-induced oxidation of the two proteins and reduction of the three flavins were studied. The quantum yields of the former and latter in the absence of oxygen are up to 0.1 and 0.04, respectively. The effects of pH and sensitizer and protein concentrations were examined in greater detail. The proposed reaction is electron transfer from the tryptophan moiety to the flavin triplet rather than excited singlet state. 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