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Great Possibilities (great + possibility)

Distribution by Scientific Domains
Life Sciences40%
Engineering40%

Selected Abstracts

Simultaneous detection of genetically modified organisms by multiplex ligation-dependent genome amplification and capillary gel electrophoresis with laser-induced fluorescence

ELECTROPHORESIS, Issue 13 2010
Virginia García-Cañas
Abstract In this work, an innovative method useful to simultaneously analyze multiple genetically modified organisms is described. The developed method consists in the combination of multiplex ligation-dependent genome dependent amplification (MLGA) with CGE and LIF detection using bare-fused silica capillaries. The MLGA process is based on oligonucleotide constructs, formed by a universal sequence (vector) and long specific oligonucleotides (selectors) that facilitate the circularization of specific DNA target regions. Subsequently, the circularized target sequences are simultaneously amplified with the same couple of primers and analyzed by CGE-LIF using a bare-fused silica capillary and a run electrolyte containing 2-hydroxyethyl cellulose acting as both sieving matrix and dynamic capillary coating. CGE-LIF is shown to be very useful and informative for optimizing MLGA parameters such as annealing temperature, number of ligation cycles, and selector probes concentration. We demonstrate the specificity of the method in detecting the presence of transgenic DNA in certified reference and raw commercial samples. The method developed is sensitive and allows the simultaneous detection in a single run of percentages of transgenic maize as low as 1% of GA21, 1% of MON863, and 1% of MON810 in maize samples with signal-to-noise ratios for the corresponding DNA peaks of 15, 12, and 26, respectively. These results demonstrate, to our knowledge for the first time, the great possibilities of MLGA techniques for genetically modified organisms analysis. [source]

Legal and ethical considerations for genetic clinical research

QUALITY ASSURANCE JOURNAL, Issue 1 2003
Judith E. Beach
Abstract From the trend in modern medicine toward the study of genes and their contribution to the development of disease has evolved an increased awareness of ,the diversity of genetic fingerprints among individuals' [1]. The incorporation of this knowledge into the technologies of the pharmaceutical industry has led to the emerging field of ,pharmacogenomics'; that is, the process of identifying the differences in genetic sequences between individuals and developing therapies [2] as ,personal medicines' [3]. For example, a drug used as a muscle relaxant during surgery, suxamethonium, was found to be lethal to patients who possessed a rare version of a gene involved in nerve transmission so that now those who receive this drug are tested for this specific gene [4]. Although pharmacogenomics promises great possibilities for the future of medicine, it does involve ethical and legal considerations that must be considered. Indeed, potential misuses of genetic information, such as discrimination in obtaining health insurance and in the workplace, need to be addressed. Genetic testing practices remain more advanced than the national and international laws governing the appropriate use of genetics. Although there is no national law in the United States that specifically addresses DNA and genetic privacy, several federal regulations would apply indirectly to the protection of this information and state legislators have successfully passed numerous state laws. Professional associations and private organizations have issued several guidelines for genetic testing practices. The purpose of this report is to provide a picture of the legal and ethical ramifications of genetic testing in clinical research. The genetic testing issue is presented herein in the categories of national, international, and state laws, policies, regulations and guidelines. Copyright © 2003 John Wiley & Sons, Ltd. [source]

Spatially explicit trophic modelling of a harvested benthic ecosystem in Tongoy Bay (central northern Chile)

AQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 6 2002
Marco Ortiz
Abstract 1.A dynamical and spatial simulation model of a harvested benthic ecosystem of central northern Chile (Tongoy Bay) was constructed using the ECOSPACE software package. 2.In this system the red alga (Chondrocanthus chamissoi), the scallop (Argopecten pupuratus), the gastropod (Xanthochorus cassidiformis) and the crab (Cancer polyodon) are harvested intensively. The impacts of harvesting these resources exclusively in the seagrass, sand-gravel, and in the sand habitats, as well as, in the seagrass and sand-gravel and in all habitats were assessed. The goal was to explore policies of sustainable exploitation of the benthic systems. 3.The most important findings were: (a) Fishing exclusively in either the seagrass or sand habitats produces a population increase in the sea star Luidia magallanica, in the seagrass Heterozostera tasmanica, and in the crab Paraxanthus barbiger. (b) Exclusive fishing in the sand-gravel habitat causes only small effects on the species and groups, which suggests that this habitat is the most resistant to harvest. (c) The simultaneous fishing on two or three habitats would produce the largest negative effect on the entire system. Therefore, a habitat rotation fishery is recommended. 4.Our study suggests that trophic-spatially explicit models offer great possibilities for the screening and planning of effective interventions or manipulations of natural systems. Copyright © 2002 John Wiley & Sons, Ltd. [source]

Seismic microzonation of the greater Bangkok area using microtremor observations

EARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 2 2004
Rabin Tuladhar
Abstract Bangkok, the capital city of Thailand, is located at a remote distance from seismic sources. However, it has a substantial risk from these distant earthquakes due to the ability of the underlying soft clay to amplify ground motions. It is therefore imperative to conduct a detailed seismic hazard assessment of the area. Seismic microzonation of big cities, like Bangkok, provides a basis for site-specific hazard analysis, which can assist in systematic earthquake mitigation programs. In this study, a seismic microzonation map for the greater Bangkok area is constructed using microtremor observations. Microtremor observations were carried out at more than 150 sites in the greater Bangkok area. The predominant periods of the ground were determined from the horizontal-to-vertical (H/V) spectral ratio technique. A microzonation map was then developed for the greater Bangkok area based on the observations. Moreover, the transfer functions were calculated for the soil profile at eight sites, using the computer program SHAKE91, to validate the results from the microtremor analysis. The areas near the Gulf of Thailand, underlaid by a thick soft clay layer, were found to have long natural periods ranging from 0.8s to 1.2s. However, the areas outside the lower central plain have shorter predominant periods of less than 0.4s. The study shows that there is a great possibility of long-period ground vibration in Bangkok, especially in the areas near the Gulf of Thailand. This may have severe effects on long-period structures, such as high-rise buildings and long-span bridges. Copyright © 2003 John Wiley & Sons, Ltd. [source]

New centralized automatic vehicle location communications software system under GIS environment

INTERNATIONAL JOURNAL OF COMMUNICATION SYSTEMS, Issue 9 2005
Omar Al-Bayari
Abstract Recent advances in wireless communications and networks have integrated relatively new technologies such as Global Positioning System (GPS), to the popular Global System for Mobile Communication (GSM), second generation cellular systems and the Geographic Information Systems (GIS) technologies. Automatic Vehicle Location (AVL) is based on a combination of GPS, GIS and telecommunication technologies. Automatic Vehicle Tracking systems are more and more used for different purposes, especially those related to tracking one vehicle or a fleet of vehicles. In this work, we introduce a new AVL system, which is based and developed under GIS software environment. The centralized software at the control station offers a new technology of transferring the intelligence of tracking system from the car unit, into the control office PC software. Centralized software will reduce the programming efforts in the car unit and will offer better fleet management. Moreover, the core of our system is based on the objects or the controllers of the GIS software, which reduces dramatically the overall system cost. Our system provides an easy access to change the functions of the system, with great possibility to satisfy the local needs. The design of our software will be presented with an explanation of the new supporting technologies that were to create the system. Finally, our software system has been validated using data from local road networks. Copyright © 2005 John Wiley & Sons, Ltd. [source]