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Granulocyte Elastase (granulocyte + elastase)

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Medical Sciences	100%

Selected Abstracts

Granulocyte elastase, matrix metalloproteinase-8 and prostaglandin E2 in gingival crevicular fluid in matched clinical sites in smokers and non-smokers with persistent periodontitis

JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 5 2002
B. Söder
Abstract Background/aims: Smokers with persistent periodontitis may have granulocytes with impaired function. This study aimed to determine the levels of granulocyte elastase, matrix metalloproteinase-8 (MMP-8) and prostaglandin E2 (PGE2) in gingival crevicular fluid (GCF) in smokers and non-smokers with persistent periodontitis. Methods: We analyzed GCF from 70 matched sites in 29 periodontitis and 6 gingivitis sites in 34 subjects, 17 smokers, and 17 non-smokers. We also analyzed separately GCF from 28 of these subjects, 14 smokers and 14 non-smokers in 14 matched periodontitis sites. The following measurements were made: elastase complexed to ,1 -antitrypsin (EA-,1AT) and MMP-8 with ELISA, functional elastase with a chromogenic substrate, and PGE2 with radioimmunoassay (125I RIA). The significance of the findings was determined with Mann-Whitney test. Results: In the 29 matched periodontitis sites, smokers had significantly more functional elastase (p<0.005) and more EA-,1AT (p<0.05) than non-smokers. In the 14 matched periodontitis sites in 14 smokers and 14 non-smokers, the former had significantly more functional elastase than the latter (p<0.001). A significant correlation was found between EA-,1AT and MMP-8 in smokers (p<0.05) and non-smokers (p<0.001) and a positive correlation between levels of functional elastase and MMP-8 in non-smokers (r2=0.98; p<0.001). Conclusions: Granulocyte function seems to be impaired in smokers with persistent periodontitis. The cells react to the bacterial challenge by releasing serine proteases, which reflect the degradation of connective tissue. The risk of progression of the disease is therefore higher in smokers with persistent periodontitis than in non-smokers. Zusammenfassung Hintergrund, Ziele: Raucher mit bestehender Parodontitis haben möglicherweise Granulozyten mit beeinträchtigter Funktion. Diese Studie zielt auf die Bestimmung der Levels von Granulozytenelastase, Matrix-Metalloproteinase-8 (MMP-8) und Prostaglandin E2 (PGE2) in der krevikulären gingivalen Flüssigkeit (GCF) bei Rauchern und Nichtrauchern mit bestehender Parodontitis. Methoden: Wir analysierten GCF von 70 entsprechenden Flächen bei 29 Parodontitis und 6 Gingivitisflächen von 34 Personen, 17 Rauchern und 17 Nichtrauchern. Wir analysierten zusätzlich getrennt die GCF von 28 dieser Personen: 14 Raucher und 14 Nichtraucher von 14 entsprechenden parodontalen Flächen. Die folgenden Messungen wurden vorgenommen: Elastasekomplex zu ,1 -Antitrypsin (EA-,1AT) und MMP-8 mit ELISA, funktionelle Elastase mit chromogenem Substrat und PGE2 mit Radioimmunoassay (125I RIA). Die Signifikanz der Ergebnisse wurde mit dem Mann-Whitney Test bestimmt. Ergebnisse: In den 29 entsprechenden parodontalen Flächen hatten die Raucher signifikant mehr funktionelle Elastase (p<0.005) und mehr EA-,1At (p<0.05) als Nichtraucher. Bei den 14 entsprechenden parodontalen Flächen der 14 Raucher und 14 Nichtraucher hatten die ersten signifikant mehr funktionelle Elastase als die letzteren (p<0.001). Eine signifikante Korrelation wurde zwischen EA-,1AT und MMP-8 bei Rauchern (p<0.05) und Nichtrauchern (p<0.001) gefunden und eine positive Korrelation zwischen den Levels der funktionellen Elastase und MMP-8 bei Nichtrauchern (r2=0.98; p<0.001) festgestellt. Schlussfolgerungen: Die Granulozytenfunktion scheint bei Rauchern mit bestehender Parodontitis beeinträchtigt zu sein. Die Zellen reagieren auf die bakterielle Herausforderung durch Freisetzung von Serinproteasen, die die Degradation von Bindegewebe reflektiert. Das Risiko einer Progression dieser Erkrankung ist deshalb bei Rauchern mit bestehender Parodontitis höher als bei Nichtrauchern. Résumé Origine, but: Les fumeurs avec parodontite persistante pourraient avoir des granulocytes ayant des fonctions déréglées. Cette étude a eu pour but de déterminer les niveaux d'élastase granulocytaire, de la métallo-protéinase-8 de la matrice (MMP-8) et de la prostaglandine E2 (PGE2) dans le fluide créviculaire gingival (GCF) chez les fumeurs et les non-fumeurs avec parodontite persistante. Méthodes: Le GCF a été prélevé de 70 sites équivalents dans 29 parodontites et 6 sites avec gingivite chez 34 sujets, 17 fumeurs et 17 non-fumeurs. Le GCF de 28 de ces sujets a été analysé séparément, 14 fumeurs et 14 non-fumeurs dans 14 sites équivalents du point de vue parodontite. Les mesures suivantes ont été relevées: l'élastase avec ,1 -antitrypsine (EA-,1AT) et MMP-8 par ELISA, l'élastase fonctionnelle avec un substrat chromogénique, et PGE2 avec un essai radio-immunitaire (125I RIA). La signification de ces découvertes a été par l'utilisation du test de Mann-Whitney. Résultats: Dans les 29 sites équivalents, les fumeurs avaient significativement plus d'élastase functionnelle (p<0.005) et plus de EA-,1AT (p<0.05) que les non-fumeurs. Dans les 14 sites équivalents du point de vue parodontite, les 14 fumeurs avaient significativement plus d'élastase fonctionnelle que les 14 non-fumeurs (p<0.001). Une relation significative a étéétablie entre EA-,1AT et MMP-8 chez les fumeurs (p<0.05) et les non-fumeurs (p<0.001) et une relation positive entre les niveaux d'élastase fonctionnelle et de MMP-8 chez les non-fumeurs (r2=0.98; p<0.001). Conclusions: La fonction granulocytaire semble être altérée chez les fumeurs avec parodontite persistante. Les cellules réagissent à l'attaque bactérienne en relâchant des protéases sérine, ce qui démontre une dégradation du tissu conjonctif. Le risque de progression de la maladie est ainsi plus élevé chez les fumeurs avec parodontite persistante que chez les non-fumeurs. [source]

Outcome of pregnancy after laser conization: Implications for infection as a causal link with preterm birth

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (ELECTRONIC), Issue 5 2008
Hitoshi Masamoto
Abstract Objective:, To investigate a causal link between infection and preterm birth in women with a shortened cervix induced by prior laser conization. Methods:, We conducted a retrospective review of the outcomes of 47 singleton pregnancies with a history of laser conization. Cervical length was measured between 17 and 23 weeks of gestation. Receiver-operating characteristic curves were used to determine the best cut-off point for the cervical length for predicting spontaneous preterm birth. We measured infectious markers in 12 women who had experienced preterm labor. Results:, Nine women had had premature labors and three had had late abortions. The mean ± SD cervical length was 33.1 ± 9.1 mm. The optimal cut-off for predicting preterm delivery was 25 mm with a sensitivity and specificity of 75.0% and 97.1%, respectively. Concentrations of granulocyte elastase were positive in five of the six samples determined. The white blood cell count and C-reactive protein levels were elevated in four out of the six patients. Sixty-seven percent of the vaginal cultures were positive for bacteria. Chorioamnionitis was present in seven women. Conclusions:, For the prediction of preterm birth in patients with a history of conization, it is helpful to look for signs of local infection when the cervical length is less than 25 mm. [source]

Coated Prostheses Are Associated With Prolonged Inflammation in Aortic Surgery: A Cost Analysis

ARTIFICIAL ORGANS, Issue 3 2008
Shunya Shindo
Abstract:, This prospective study was conducted to compare inflammatory responses between patients receiving coated and uncoated vascular prostheses, and to examine their effect on length of stay and cost of patients undergoing abdominal aortic aneurysmectomy. Patients undergoing elective vascular reconstruction of an abdominal aortic aneurysm were assigned randomly to coated-graft or uncoated-graft groups (n = 20, for each group). Interleukin (IL)-6, granulocyte elastase, white blood cell count, C-reactive protein (CRP), and body temperature (BT) were prospectively recorded preoperatively and on postoperative days (PODs) 1, 3, 7, and 14. In-hospital stay and hospitalized costs were also analyzed. IL-6 and CRP concentrations in the coated-graft group were higher than those in the uncoated-graft group (P = 0.01 and 0.05). BT was more frequently elevated >37°C at POD 14 in the coated-graft group than in the uncoated-graft group (P =0.03). Discharge was delayed, and overall hospitalization cost was higher in the coated-graft group than in the uncoated group (17.6 vs. 13.5 days, and 2 010 000 vs. 1 780 000 yen, P = 0.006 and P = 0.002, respectively). Coated vascular prosthesis demonstrated more profound inflammatory reaction than noncoated prosthesis, postoperatively. [source]