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Germplasm Characterization (germplasm + characterization)
Selected AbstractsIsolation and characterization of microsatellite loci from a commercial cultivar of Musa acuminataMOLECULAR ECOLOGY RESOURCES, Issue 2 2006SILVANA CRESTE Abstract A genomic library from the commercial diploid cultivar ,Ouro' (Musa acuminata), enriched for CT- and GT-repeats, was used to isolate and characterize 23 microsatellite loci. These loci were tested in 10 Musa genotypes, representing various Musa genomic groups with distinct ploidy level. The number of alleles per locus ranged from one to seven, and 20 loci were highly informative. Four loci appeared to amplify B genome-specific alleles, while three loci seemed to be absent in the B genome. The polymorphism revealed by these loci will be extremely useful for genetic mapping, marker-assisted selection, germplasm characterization and evolutionary studies in Musa. [source] Novel microsatellite markers for Bactris gasipaes (Palmae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2004D. P. RODRIGUES Abstract Ten new polymorphic microsatellites were isolated and characterized in Bactris gasipaes using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. The loci are highly polymorphic, with a mean of 14.6 alleles per locus and a mean expected heterozygosity of 0.83 among 62 individuals of the Pampa Hermosa landrace. These microsatellites will be useful for population genetic analysis and germplasm characterization for heart-of-palm breeding. [source] Development of DNA microsatellite markers in the Andean root crop arracacha: Arracacia xanthorrhiza Banc. (Apiaceae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2004E. MORILLO Abstract A microsatellite-enriched library was constructed in the Andean root crop arracacha (Arracacia xanthorriza B). Of 18 loci tested, 14 were found to be polymorphic after screening for diversity in different cultivars and related wild forms. Allelic diversity in the crop was low but the transferability of the primers to closely related wild forms was good. The loci reported here are the first genetic markers to be published for this species and will be useful for future germplasm characterization and studies of genetic diversity. [source] A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterizationANNALS OF APPLIED BIOLOGY, Issue 3 2003H D MIGNOUNA Summary Several DNA-based marker systems are available for genetic fingerprinting of plants but information on their relative usefulness for yam germplasm characterisation is lacking. The efficiency of RAPD, AFLP and SSR markers for the assessment of genetic relationships, and for cultivar identification and discrimination among 45 West and Central African white yam cultivars belonging to 22 morphotypes/cultivar groups was investigated. Dendrograms were produced based on band pattern scores using the UPGMA method. Results showed that each of the three techniques could unequivocably identify each cultivar, but that techniques differed in the mean number of profiles generated per primer (or primer pair) per cultivar, referred to as genotype index (GI). The order of merit based on this criterion in this study was AFLPs (GI = 2.56), SSRs (GI = 0.39) and RAPDs (GI = 0.35). Yam genotypes classified in the same cultivar group based on morphology were often genetically different, emphasising the need for molecular fingerprinting in yam germplasm characterisation. AFLPs showed the highest efficiency in detecting polymorphism and revealed genetic relationships that most closely reflected morphological classification. [source] | ||||||||||