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Genetically Modified Soybean (genetically + modified_soybean)
Selected AbstractsDispersal and egg shortfall in Monarch butterflies: what happens when the matrix is cleaned up?ECOLOGICAL ENTOMOLOGY, Issue 1 2010MYRON P. ZALUCKI 1. We use an individual-based model describing the life of a monarch butterfly, which utilises milkweeds both aggregated in patches and scattered across the wider landscape as a substrate for laying eggs. The model simplifies the metapopulation of milkweed habitat patches by representing them as a proportion of the overall landscape, with the rest of the landscape considered matrix, which may contain some low density of milkweed plants. 2. The model simulates the number of eggs laid daily by a butterfly as it searches for hosts. The likelihood of finding hosts is related to the density of plants and the search ability of the butterfly. For an empty matrix, remaining in a habitat patch results in more eggs laid. However individuals that are good searchers have almost equivalent success without remaining in a habitat patch. These individuals are most affected by the presence of hosts in the matrix. 3. Given realistic values of habitat patch availability, our model shows that the presence of plants at a low density in the matrix has a substantial impact on the number of eggs laid; removing these plants can reduce lifetime potential fecundity by ca. 20%. These results have implications for monarch butterflies inhabiting agricultural landscapes, in which genetically modified soybean that is resistant to herbicides has resulted in the decimation of milkweeds over large areas. [source] Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybeanINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 10 2007Cibele dos Santos Ferrari First page of article [source] Pancreatic response of rats fed genetically modified soybeanJOURNAL OF APPLIED TOXICOLOGY, Issue 2 2008Javier A. Magaña-Gómez Abstract Mice fed genetically modified (GM) soybean were not affected in nutritional performance, but pancreatic microscopic features were disturbed. The mechanisms for these contradictory findings are unknown. This study analysed the histology of acinar pancreatic cells and the expression of pancreatitis-associated protein (PAP) and trypsinogen mRNA in rats fed GM soy protein. Two bioassays were run, each one with 34 Wistar rats distributed into two groups fed with non-GM or GM-soy protein (18% protein) for 0, 1, 3, 5, 15 and 30 days. Nutritional evaluation, plasma amylase levels, pancreatic histological analysis and quantification of PAP and trypsinogen mRNAs levels using quantitative real-time RT-PCR were done. No differences in nutritional performance among rats fed non-GM and GM diets were found. The GM, but not the non-GM, diet induced zymogen-granule depletion after 15 days feeding, returning to normal levels after 30 days (P < 0.05). Acinar disorganization started as early as 5 days after initiation of the GM diet and it recovered after 30 days. Levels of PAP mRNA significantly increased in the GM diet between day 1 and day 3 and decreased to the basal level by day 15. Trypsinogen mRNA peaked at two different times; at day 1 and at day 15, decreasing to basal levels after 30 days. Plasma amylase levels remained unchanged at all times. This indicates that GM soy protein intake affected pancreas function, evidenced by the early acute PAP mRNA increased levels and pancreas cellular changes followed by recuperation of acinar cells after 30 days. Copyright © 2007 John Wiley & Sons, Ltd. [source] CE-TOF MS analysis of complex protein hydrolyzates from genetically modified soybeans , A tool for foodomicsELECTROPHORESIS, Issue 7 2010Carolina Simó Abstract A CE-TOF MS proteomic approach was applied for the analysis of hydrolyzates from complex soybean protein mixtures. After CE-TOF MS method development, the new approach provided the simultaneous analysis of more than 150 peptides from the soybean protein fraction soluble in ACN-water (80/20,v/v). The method is fast (about 30,min of analysis per sample) and is characterized by a relatively low running cost. The approach was used to study the substantial equivalence between a genetically modified variety of soybean compared with its traditional counterpart. No significant differences were found between the two studied soybeans based on the protein fraction studied. The capacity of the CE-TOF MS method to analyze complex mixtures of peptides in short times opens interesting possibilities in the growing Foodomics area. [source] Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)AQUACULTURE RESEARCH, Issue 12 2009Tao Ran Abstract We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10,10 g ,L,1 DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non-GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal. [source] | |||||||||||||