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GPI Antibodies (gpi + antibody)

Distribution by Scientific Domains
Medical Sciences100%

Selected Abstracts

The Investigation on the Value of Repeat and Combination Test of ACA and Anti-,2 -GPI Antibody in Women with Recurrent Spontaneous Abortion

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 4 2008
Shi Hua Bao
Problem In order to investigate the value of anticardiolipin antibodies (ACA) and anti-,2 -GPI antibodies detection in screening autoimmune type recurrent spontaneous abortion and its clinic application in antiphospholipid syndrome diagnosis, we adopt repeat combined ACA and anti-,2 -GPI antibodies detection in this study. Method of study Sera were collected from patients and work-up was done for detection of ACA and anti-,2 -GPI antibodies by enzyme-linked immunosorbent assay (ELISA). The work-up was done for detection of antibodies once in every 6 weeks for 14 times consecutively. Results The repeated and combined detection of ACA and anti-,2 -GPI antibodies detection could raise the positivity rate up to 21.8% (P < 0.05) in comparison with positive for ACA alone (14.1%), positive for anti-,2 -GPI alone (3.1%), and concurrently positive for both ACA and anti-,2 -GPI antibodies (4.6%). In 91 confirmed positive antiphospholipid antibodies (APA) patients, with more frequent screening for ACA and anti-,2 -GPI antibodies, more patients with APA were found. The positive rate of five and more screenings was over 81.32%, which was statistically significant (P < 0.05), in comparison with that of four or less screenings (68.13%). Conclusion Our data implied that it would be appropriate to take over five or more screenings of combined ACA and anti-,2 -GPI antibodies detection in suspect patients to facilitate the positive diagnostic rate for autoimmune type RSA. [source]

Diagnosis of lupus anticoagulant in the lupus anticoagulant-hypoprothrombinemia syndrome: Report of two cases and review of the literature

AMERICAN JOURNAL OF HEMATOLOGY, Issue 3 2002
Vicente Baca
Abstract We report a severe hemorrhagic disorder in two pediatric patients with lupus anticoagulant (LA) associated to acquired factor II (prothrombin) deficiency. In both patients, hemorrhagic symptoms resolved after corticosteroid therapy. Serial coagulation studies showed that Staclot LA® assay was more sensitive than DVVconfirm® and Staclot PNP® tests to confirm the presence of LA when associated with severe factor II deficiency. Both patients had non-neutralizing anti-prothrombin antibodies and their titers inversely correlated with factor II activity (r = ,1.0, P < 0.0001). Associated findings in these patients included positive immunologic tests for systemic lupus erythematosus, a positive anti-cardiolipin antibody, and anti-,2 GPI antibodies in one case. Our findings point out the difficulty in diagnosing LA associated with acquired factor II deficiency and suggest that, in confirmation of its phospholipid dependency, the inclusion of a source of normal human plasma in the test sequence to correct for any factor deficiency and a confirmatory step utilizing hexagonal (II) phase phospholipids may be crucial to the diagnosis of LA in some patients with LA-hypoprothrombinemia syndrome. Am. J. Hematol. 71:200,207, 2002. © 2002 Wiley-Liss, Inc. [source]

The Investigation on the Value of Repeat and Combination Test of ACA and Anti-,2 -GPI Antibody in Women with Recurrent Spontaneous Abortion

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 4 2008
Shi Hua Bao
Problem In order to investigate the value of anticardiolipin antibodies (ACA) and anti-,2 -GPI antibodies detection in screening autoimmune type recurrent spontaneous abortion and its clinic application in antiphospholipid syndrome diagnosis, we adopt repeat combined ACA and anti-,2 -GPI antibodies detection in this study. Method of study Sera were collected from patients and work-up was done for detection of ACA and anti-,2 -GPI antibodies by enzyme-linked immunosorbent assay (ELISA). The work-up was done for detection of antibodies once in every 6 weeks for 14 times consecutively. Results The repeated and combined detection of ACA and anti-,2 -GPI antibodies detection could raise the positivity rate up to 21.8% (P < 0.05) in comparison with positive for ACA alone (14.1%), positive for anti-,2 -GPI alone (3.1%), and concurrently positive for both ACA and anti-,2 -GPI antibodies (4.6%). In 91 confirmed positive antiphospholipid antibodies (APA) patients, with more frequent screening for ACA and anti-,2 -GPI antibodies, more patients with APA were found. The positive rate of five and more screenings was over 81.32%, which was statistically significant (P < 0.05), in comparison with that of four or less screenings (68.13%). Conclusion Our data implied that it would be appropriate to take over five or more screenings of combined ACA and anti-,2 -GPI antibodies detection in suspect patients to facilitate the positive diagnostic rate for autoimmune type RSA. [source]

Targeted mast cell silencing protects against joint destruction and angiogenesis in experimental arthritis in mice

ARTHRITIS & RHEUMATISM, Issue 6 2007
Manfred Kneilling
Objective Induction of arthritis with autoantibodies against glucose-6-phosphate isomerase (GPI) is entirely independent of T cells and B cells but is strictly dependent on the presence of mast cells. Here, we used this disease model to analyze whether exclusive intraarticular mast cell reconstitution is sufficient for disease induction and whether targeted mast cell silencing can prevent neoangiogenesis and joint destruction, 2 hallmarks of rheumatoid arthritis. Methods Ankle swelling and clinical index scores were determined after injection of either K/BxN mouse,derived serum or control serum in wild-type Kit+/Kit+ mice, congenic mast cell,deficient KitW/KitW - v mice, or mast cell,deficient KitW/KitW - v mice reconstituted with mast cells, either by intraperitoneal or selective intraarticular injection. Angiogenesis was quantified in vivo by measuring activated ,v,3 integrin using 18F,galacto-RGD and positron emission tomography. In addition, staining of joint tissue with hematoxylin and eosin, Giemsa, ,3, and ,-actin was performed. The effect of mast cell stabilization by treatment with cromolyn or salbutamol was investigated in C57BL/6 or BALB/c mice. Results Comparing wild-type mice, mast cell,deficient KitW/KitW - v mice, and mast cell,reconstituted KitW/KitW - v mice, we first showed that intraarticular and intraperitoneal mast cell engraftment fully restores susceptibility to antibody-induced arthritis, angiogenesis, and ,v,3 integrin activation. Importantly, selective mast cell silencing with either salbutamol or cromolyn prevented ,v,3 integrin activation, angiogenesis, and joint destruction. Conclusion Mast cell engraftment fully restores susceptibility to ,v,3 integrin activation, angiogenesis, and joint destruction in GPI antibody,induced arthritis. Importantly, selective mast cell stabilization prevents ,v,3 integrin activation, angiogenesis, and joint destruction. [source]