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GCF IL-1 (gcf + il-1)
Selected AbstractsInterleukin-1, levels in gingival crevicular fluid and serum under naturally occurring and experimentally induced gingivitisJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 8 2010Leonardo Trombelli Trombelli L, Scapoli C, Carrieri A, Giovannini G, Calura G, Farina R. Interleukin-1, levels in gingival crevicular fluid and serum under naturally occurring and experimentally induced gingivitis. J Clin Periodontol 2010; 37: 697-704 doi: 10.1111/j.1600-051X.2010.01573.x. Abstract Aims: To evaluate the interleukin-1, (IL-1,) levels in gingival crevicular fluid (GCF) and serum in either naturally occurring (N-O) or experimentally induced (E-I) plaque-associated gingivitis. Material and Methods: Thirty-seven periodontally healthy subjects were evaluated in real life conditions (N-O gingivitis) as well as after 21 days of experimental gingivitis trial (E-I gingivitis). During the experimental gingivitis trial, in one maxillary quadrant (test quadrant), gingival inflammation was induced by oral hygiene abstention, while in the contralateral (control) quadrant, oral hygiene was routinely continued. IL-1, concentrations in N-O and E-I gingivitis were investigated for IL-1B+3954 and IL-1B,511 gene polymorphisms. Results: (i) GCF IL-1, concentrations in E-I gingivitis were significantly higher compared with N-O gingivitis; (ii) an intra-individual correlation between GCF concentrations of IL-1, detected in N-O and E-I gingivitis was observed in control quadrants, but not in test quadrants; (iii) IL-1, concentration in GCF was associated with IL-1B+3954 genotype only at test quadrants; (iv) IL-1, was detectable in serum only at low levels in a limited number of subjects, without difference between gingivitis conditions. Conclusions: Aspects of the bacterial challenge to the gingival tissues, such as the amount of plaque deposits and plaque accumulation rate, appear to affect the IL-1, levels in GCF in subjects with a specific IL-1B genotype. [source] Gingival changes during pregnancy: I. Influence of hormonal variations on clinical and immunological parametersJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 3 2010Elena Figuero Figuero E, Carrillo-de-Albornoz A, Herrera D, Bascones-Martínez A. Gingival changes during pregnancy: I. Influence of hormonal variations on clinical and immunological parameters. J Clin Periodontol 2009; 37: 220,229. doi: 10.1111/j.1600-051X.2009.01516.x. Abstract Aim: To test whether exacerbated gingival inflammation in pregnancy is associated with increased salivary hormone levels and changes in gingival crevicular fluid (GCF) interleukin-1, (IL-1,) and prostaglandin-E2 (PGE2) levels. Material and methods: In this cohort study, 48 pregnant women without periodontitis were evaluated in the first, second, and third trimesters and at 3 months postpartum. Twenty-eight non-periodontitis non-pregnant women were evaluated twice, with a 6-month interval. Plaque and gingival indices (PlI, GI), salivary progesterone and estradiol and GCF IL-1, and PGE2 levels were determined. anova for repeated measures or Friedman's test were used for intragroup analyses. Inter-group comparisons were analysed with t -test or Mann,Whitney U -test. Correlations were evaluated with Pearson's and Spearman's test. Results: Pregnant women showed an increase in GI (p<0.05) despite maintaining low PlI values. No changes in IL-1, and PGE2 levels were observed during pregnancy. No significant correlation was found between the GI increase and salivary hormone levels. GI (p<0.05) and IL-1, levels (p<0.001) were lower in non-pregnant than in pregnant women. Conclusions: This study confirms the presence of an exacerbated gingival inflammation during pregnancy, but this phenomenon could not be associated with an increase in progesterone or estradiol or with changes in PGE2 or IL-1,. [source] Gingival crevicular fluid interleukin-1,, prostaglandin E2 and periodontal status in a community populationJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 4 2007Y. Zhong Abstract Aim: Interleukin-1 , (IL-1,) and prostaglandin E2 (PGE2) are key inflammatory mediators involved in periodontal disease. The purposes of this molecular cross-sectional epidemiological study were to investigate relationships in a community sample between mean concentrations of IL-1, and PGE2 in gingival crevicular fluid (GCF) and (1) clinical periodontal signs and (2) risk factors of host inflammatory response and/or periodontal disease. Material and Methods: The sample comprised 6277 community-dwelling adults aged 52,74 years enrolled in the Atherosclerosis Risk in Communities (ARIC) study. IL-1, and PGE2 concentrations were measured using enzyme-linked immunosorbent assay. Person-level summary variables were computed for maximum pocket depth (MaxPD), maximum clinical attachment level (MaxCAL) and presence/absence of bleeding on probing (BOP). Mean GCF IL-1, and PGE2 concentrations were dependent variables in multiple linear regression models with periodontal measures and covariates as explanatory variables. Results: Both GCF IL-1, and PGE2 were positively related to MaxPD and BOP in multiple regression models (p<0.01). Increased levels of IL-1, and PGE2 were associated with body mass index 30 kg/m2. Conclusion: Higher levels of GCF IL-1, and PGE2 were significantly associated with clinical signs of periodontal disease and independently related to patient-based anthropomorphic measures, behaviours and exposures in community-dwelling adults. [source] Plasma levels of tumour necrosis factor- , in patients with chronic periodontitis and type 2 diabetesJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 1 2007S. Engebretson Abstract Objectives: Studies suggest that elevated circulating tumour necrosis factor- , (TNF- ,) may contribute to insulin resistance in patients with type 2 diabetes. The source of plasma TNF has been thought to be adipocytes associated with obesity, but inflammation and infection result in TNF- , production as well. Methods: We studied 46 patients with type 2 diabetes and chronic periodontitis to determine the relationship between plasma TNF- , levels and clinical measures of periodontitis, gingival crevicular fluid (GCF) interleukin-1, (IL-1,), plasma endotoxin, serum glucose, and glycated haemoglobin (HbA1c). TNF- , levels were measured using a high sensitivity enzyme-linked immunosorbent assay. Results: TNF- , showed a significant positive correlation with attachment loss (r=0.40, p=0.009), plasma endotoxin (r=0.33, p=0.03), and GCF IL-1, (r=0.33, p=0.035), but not probing depth (r=0.28, p=0.07), bleeding on probing (r=0.30, p=0.053), plaque index (r=0.22, p=0.17), serum glucose, HbA1c (r=0.10, p=0.50), or body mass index (r=0.077, p=0.62). A dose,response relationship was observed between periodontitis severity and TNF- , (p=0.012). Conclusion: The finding that chronic periodontitis is associated with plasma TNF- , levels in subjects with type 2 diabetes supports the hypothesis that periodontal infection and inflammation may contribute to insulin resistance. [source] Biomarkers of periodontal inflammation in the Australian adult populationAUSTRALIAN DENTAL JOURNAL, Issue 2 2009TR Fitzsimmons Abstract Background:, Several inflammatory biomarkers are implicated in the pathogenesis of periodontitis including interleukin-1, (IL-1,) and C-reactive protein (CRP). This study investigated the presence of these factors in gingival crevicular fluid (GCF) and their relationship to clinical and social determinants of periodontitis in the Australian population. Methods:, Equal numbers of periodontitis cases and non-cases were sampled during oral epidemiologic examination in the National Survey of Adult Oral Health. GCF was sampled from four sites where probing pocket depth (PPD) and recession were recorded. From these, IL-1, and CRP were quantified by ELISA and the log amount of GCF IL-1, (pg) per person and the proportion of adults with detectable CRP was computed. Results:, Periodontitis cases (n = 511) had significantly higher levels of IL-1, and CRP than non-cases (n = 562). PPD, clinical attachment loss, plaque and gingivitis indices were positively associated with elevated levels of both biomarkers. Levels of both were positively associated with age, low socio-economic position and non-Australian birth. Conclusions:, The presence of IL-1, and CRP in GCF are associated with periodontal disease parameters within the Australian population. The levels of both biomarkers are influenced by age, education and eligibility for public dental care. [source] | ||||||||||