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Future Tests (future + test)
Selected AbstractsInhibition of acetylcholinesterase by physostigmine analogs: Conformational mobility of cysteine loop due to the steric effect of the alkyl chainJOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, Issue 2 2002Enrico Gavuzzo Abstract The effect of a series of physostigmine analogs on acetylcholinesterase activity was investigated. The second-order rate constant kon of the enzyme,inhibitor complex correlates with the conformational positioning of aromatic residues, especially Trp84, in the transition state complex. The van der Waals interactions are an important structural element of this conformational change. A transient mobility of the cysteine loop (Cys67,Cys94) was confined only to the presence of a significant steric effect. Even with this limitation, however, the steric effect seems to be an appropriate model for future tests on the "back door" hypothesis involving facilitated opening for faster product clearance. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:64,69, 2002; Published online in Wiley Interscience (www.interscience.wiley.com). DOI 10.1002/jbt.10026 [source] Testing the abundant-centre hypothesis using intertidal porcelain crabs along the Chilean coast: linking abundance and life-history variationJOURNAL OF BIOGEOGRAPHY, Issue 3 2010Marcelo M. Rivadeneira Abstract Aim, The abundant-centre hypothesis (ACH) is based on the assumption that physiological constraints limit populations at the edges of their distributional range, yet the geographical variation of physiological performance or life-history traits has rarely been examined. Here we examine the applicability of the ACH in a marine system by testing whether physiological predictions are reflected in large-scale variations of life-history traits. Location, The Chilean coast (18°,42° S), encompassing more than 2500 km along the Pacific coast of South America. Methods, Five porcelain crab species (Petrolisthes granulosus, Petrolisthes laevigatus, Petrolisthes tuberculatus, Petrolisthes violaceus and Allopetrolisthes angulosus) were sampled on intertidal boulder beaches at 13 sampling sites. For each species and site we evaluated: (1) relative abundance (density), (2) maximum size, (3) size at maturity, (4) sex ratio, (5) proportion of ovigerous females, and (6) presence of recruits. The shape of the spatial distribution of each trait was evaluated statistically against the prediction of four hypothetical models (normal, ramped-south, ramped-north and abundant-edge). Results, The relative abundance and life-history traits showed different spatial patterns among species. Relative abundance (across sites) was fitted by a normal model in only two species. No model fitted the spatial variation in body size and size at first maturity, which showed a slight but monotonic poleward increase in all species. Sex ratio showed a prominent hump-shaped pattern, with females prevailing in the centre of the ranges and males dominating towards the range boundaries; this pattern was statistically significant in three of the five studied species. The proportion of ovigerous females showed no clear latitudinal trends, and mature individuals were observed across most of the geographical range of the species. However, recruits tended to be absent towards the southern (poleward) boundaries of the distribution. Main conclusions, The ACH does not apply to all species equally. The link between abundance and life-history traits is complex and variable among the porcelain crab species studied. Overall, the observed patterns were consistent with the idea that equatorward boundaries might be controlled by physiological restrictions mainly affecting adult survival, whereas poleward boundaries might be shaped by limitations in reproductive output and larval survival. Our results underline the importance of incorporating ecological, physiological and life-history studies in future tests of the ACH. [source] MHC studies in nonmodel vertebrates: what have we learned about natural selection in 15 years?JOURNAL OF EVOLUTIONARY BIOLOGY, Issue 3 2003L. Bernatchez Abstract Elucidating how natural selection promotes local adaptation in interaction with migration, genetic drift and mutation is a central aim of evolutionary biology. While several conceptual and practical limitations are still restraining our ability to study these processes at the DNA level, genes of the major histocompatibility complex (MHC) offer several assets that make them unique candidates for this purpose. Yet, it is unclear what general conclusions can be drawn after 15 years of empirical research that documented MHC diversity in the wild. The general objective of this review is to complement earlier literature syntheses on this topic by focusing on MHC studies other than humans and mice. This review first revealed a strong taxonomic bias, whereby many more studies of MHC diversity in natural populations have dealt with mammals than all other vertebrate classes combined. Secondly, it confirmed that positive selection has a determinant role in shaping patterns of nucleotide diversity in MHC genes in all vertebrates studied. Yet, future tests of positive selection would greatly benefit from making better use of the increasing number of models potentially offering more statistical rigour and higher resolution in detecting the effect and form of selection. Thirdly, studies that compared patterns of MHC diversity within and among natural populations with neutral expectations have reported higher population differentiation at MHC than expected either under neutrality or simple models of balancing selection. Fourthly, several studies showed that MHC-dependent mate preference and kin recognition may provide selective factors maintaining polymorphism in wild outbred populations. However, they also showed that such reproductive mechanisms are complex and context-based. Fifthly, several studies provided evidence that MHC may significantly influence fitness, either by affecting reproductive success or progeny survival to pathogens infections. Overall, the evidence is compelling that the MHC currently represents the best system available in vertebrates to investigate how natural selection can promote local adaptation at the gene level despite the counteracting actions of migration and genetic drift. We conclude this review by proposing several directions where future research is needed. [source] Twenty-seven new microsatellites for the migratory Asian catfish family PangasiidaeMOLECULAR ECOLOGY RESOURCES, Issue 1 2002Zeb S. Hogan Abstract In this paper, we describe primers for 27 new microsatellite loci tested on five species of migratory Asian catfish: Pangasius krempfi, P. bocourti, P. conchophilus, P. pleurotaenia, and Helicophagus waandersii. These primers were developed from a (GATA)n library created from the pooled DNA of three species of pangasiid catfish. All 27 loci are polymorphic in at least one species. Fifteen loci are polymorphic in at least three species. The primers described in this paper are thus shown to be useful in several species within the catfish family Pangasiidae, and may prove useful in additional species in future tests. [source] Current recommendations for the molecular evaluation of newly diagnosed holoprosencephaly patients,,AMERICAN JOURNAL OF MEDICAL GENETICS, Issue 1 2010Daniel E. Pineda-Alvarez§ Abstract Holoprosencephaly (HPE) is the most common structural malformation of the developing forebrain in humans and is typically characterized by different degrees of hemispheric separation that are often accompanied by similarly variable degrees of craniofacial and midline anomalies. HPE is a classic example of a complex genetic trait with "pseudo"-autosomal dominant transmission showing incomplete penetrance and variable expressivity. Clinical suspicion of HPE is typically based upon compatible craniofacial findings, the presence of developmental delay or seizures, or specific endocrinological abnormalities, and is then followed up by confirmation with brain imaging. Once a clinical diagnosis is made, a thorough genetic evaluation is necessary. This usually includes analysis of chromosomes by high-resolution karyotyping, clinical assessment to rule-out well recognized syndromes that are associated with HPE (e.g., Pallister-Hall syndrome, Smith-Lemli-Opitz syndrome and others), and molecular studies of the most common HPE associated genes (e.g., SHH, ZIC2 and SIX3). In this review, we provide current step-by-step recommendations that are medically indicated for the genetic evaluation of patients with newly diagnosed HPE. Moreover, we provide a brief review of several available methods used in molecular diagnostics of HPE and describe the advantages and limitations of both currently available and future tests as they relate to high throughput screening, cost, and the results that they may provide. Published 2010 Wiley-Liss, Inc. [source] Serological testing for Bartonella henselae infections in The Netherlands: clinical evaluation of immunofluorescence assay and ELISACLINICAL MICROBIOLOGY AND INFECTION, Issue 6 2007M. J. Vermeulen Abstract Cat-scratch disease (CSD), caused by Bartonella henselae infection, can mimic malignancy and can manifest atypically. Reliable serological testing is therefore of great clinical importance. The diagnostic performance of immunofluorescence assay (IFA) and ELISA was evaluated in a group of Dutch patients with proven CSD (clinical diagnosis confirmed by PCR). Sera of 51 CSD patients and 56 controls (patients with similar symptoms, but who were B. henselae PCR-negative and had an alternative confirmed diagnosis) were tested for anti- B. henselae IgM and IgG by IFA and ELISA. A commercially available IFA test for IgM had a sensitivity of 6%. In-house assays for IgM showed specificities of 93% (IFA) and 91% (ELISA), but with low sensitivities (53% and 65%, respectively). With a specificity of 82% (IFA) and 91% (ELISA), in-house IgG testing showed a significantly higher sensitivity in IFA (67%) than in ELISA (28%, p <0.01). Sensitivity was higher for genotype I (38,75%) than for genotype II (7,67%) infections, but this was only statistically significant for IgG ELISA (p <0.05). In conclusion, detection of IgM against B. henselae by in-house ELISA and IFA was highly specific for the diagnosis of CSD. The high seroprevalence in healthy individuals limits the clinical value of IgG detection for diagnosing CSD. Given the low sensitivity of the serological assays, negative serology does not rule out CSD and warrants further investigation, including PCR. Adding locally isolated (e.g., genotype II) B. henselae strains to future tests might improve the sensitivity. [source] | ||||||||||