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Fusarium Solani (fusarium + solani)

Distribution by Scientific Domains
Medical Sciences35%
Life Sciences35%
Chemistry21%

Selected Abstracts

Pathogenicity of fungi isolated from Quercus suber in Catalonia (NE Spain)

FOREST PATHOLOGY, Issue 5 2000
J. Luque
Summary Thirty-four fungal species isolated from cork oak (Quercus suber) in Catalonia (NE Spain) during 1992,95 were tested for pathogenicity either in stem, leaf or root inoculations. Eleven species were found to be pathogenic on stem: Biscogniauxia mediterranea, Botryosphaeria stevensii, Diatrype cf. stigma, Endothia gyrosa, Fusarium solani, Graphium sp., Ophiostoma quercus, Phomopsis sp., Phytophthora cinnamomi, Sporendocladia bactrospora and an unidentified Coelomycete. Three fungi showed pathogenic effects on leaves: Dendrophoma myriadea, Lembosia quercina and Phomopsis quercella. No clear pathogenic effects were detected in the root inoculation experiment. Trunk pathogens were differentiated into two groups according to the effects induced in the inoculated plants; B. stevensii, Phomopsis sp. and P. cinnamomi caused the death of the inoculated plants and induced the formation of large cankers and vascular necroses. The other pathogenic species also produced severe cankers and vascular lesions, but no significant mortality was detected. Water stress increased the lesions caused by B. mediterranea and Phomopsis sp., but limited those of P. cinnamomi and the rest of the inoculated fungi. However, water stress did not significantly affect the damage caused by B. stevensii, which was the most virulent of the species tested. Leaf pathogens only showed their effects if the leaf cuticle was previously damaged. Lembosia quercina caused small dark lesions whereas D. myriadea and P. quercella produced large necrotic areas in well-watered plants. The lesions caused by the last two fungi were reduced by water stress. Résumé Le pouvoir pathogène de trente-quatre espèces fongiques isolées de chêne liège en Catalogne (nord-est de l'Espagne) de 1992 à 1995 a été testé par inoculation sur tronc, feuilles et racines. Onze espèces se sont montrées pathogènes sur tronc: Biscogniauxia mediterranea, Botryosphaeria stevensii, Diatrype cf. stigma, Endothia gyrosa, Fusarium solani, Graphium sp., Ophiostoma quercus, Phomopsis sp., Phytophthora cinnamomi, Sporendocladia bactrospora et un Coelomycète non identifié. Trois champignons ont eu un effet pathogène sur feuilles: Dendrophoma myriadae, Lembosia quercina et Phomopsis quercella. Aucun effet clair n'a été détecté chez les inoculations de racines. Les pathogènes de tronc se répartissaient en deux groupes selon leurs effets en inoculation; B. stevensii, Phomopsis sp. et P. cinnamomi provoquaient la mort des plants et induisaient le formation de grands chancres et des nécroses vasculaires. Les autres espèces pathogènes produisaient aussi des chancres graves et des lésions vasculaires, mais pas de mortalité significative. Un stress hydrique augmentait les lésions provoquées par B. mediterranea et Phomopsis sp. mais limitait ceux de P. cinnamomi et des autres champignons inoculés. Cependant, le stress hydrique n'affectait pas significativement les dégâts par B. stevensii qui était la plus agressive des espèces testées. Les pathogènes foliaires n'avaient d'effet que si la cuticule foliaire était préalablement endommagée. Lembosia quercina provoquait de petites lésions sombres et D. myriadea et P. quercella provoquaient de grandes plages nécrotiques chez les plants bien arrosés; les lésions causées par ces deux derniers champignons étaient réduites par le stress hydrique. Zusammenfassung Die Pathogenität von 34 Pilzarten, die im Zeitraum 1992,1995 von Korkeichen (Quercus suber) in Katalonien (NO-Spanien) isoliert wurden, wurden mit Hilfe von Trieb-, Blatt- oder Wurzelinokulationen untersucht. Am Stamm erwiesen sich 11 Arten als pathogen: Biscogniauxia mediterranea, Botryosphaeria stevensii, Diatrype cf. stigma, Endothia gyrosa, Fusarium solani, Graphium sp., Ophiostoma quercus, Phomopsis sp., Phytophthora cinnamomi, Sporendocladia bactrospora und ein nicht identifizierter Coelomycet. Drei Arten verursachten Symptome auf Bla¨ttern: Dendrophoma myriadea, Lembosia quercina und Phomopsis quercella. Bei den Wurzelinokulationen wurden keine pathogenen Effekte beobachtet. Bei den Stammpathogenen wurden nach den von ihnen an den inokulierten Pflanzen verursachten Symptomen zwei Gruppen unterschieden: B. stevensii, Phomopsis sp. und P. cinnamomi verursachten den Tod der Pflanzen und induzierten die Bildung von grossen Rinden- und Xylemnekrosen. Die anderen pathogenen Arten verursachten ebenfalls starke Rindennekrosen und Gefa¨ssla¨sionen, es wurde jedoch keine auffallende Mortalita¨t beobachtet. Unter Wasserstress war die durch B. mediterranea und Phomopsis sp. induzierte Nekrosebildung versta¨rkt, dagegen war sie bei P. cinnamomi und den u¨brigen inokulierten Pilzen reduziert. Wasserstress beeinflusst jedoch das Ausmass der Scha¨digung durch B. stevensii, der virulentesten der untersuchten Arten, nicht. Die Blattpathogene verursachten nur dann Symptome, wenn zuvor die Blattcuticula bescha¨digt worden war. Lembosia quercina verursachte kleine dunkle La¨sionen, wa¨hrend D. myriadea und P. quercella bei gut bewa¨sserten Pflanzen grosse Nekrosen verursachten. Diese Symptome waren unter Wasserstress weniger stark ausgepra¨gt. [source]

A Thermostable Chitinase with Chitin-Binding Activity from Phaseolus limensis

JOURNAL OF FOOD SCIENCE, Issue 6 2008
S.Y. Wang
ABSTRACT:, A 28.6-kDa chitinase with chitin-binding activity was isolated from the large lima bean (Phaseolus limensis) seeds. The procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on Affi-gel blue gel, and high-performance liquid chromatography (HPLC) on SP-Toyopearl. There was an almost 108-fold increase in specific activity of the purified chitinase compared with that of the crude extract. The enzyme exhibited a pI of 7.8 by isoelectric focusing electrophoresis. The optimum pH and the optimum temperature for activity toward N-acetyld-glucosamine were 5.4 and 40 to 50 °C, respectively. The enzyme was stable up to 55 °C. It exerted a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii. [source]

Suppression of Root Rotting Fungi and Root Knot Nematode of Chili by Seaweed and Pseudomonas aeruginosa

JOURNAL OF PHYTOPATHOLOGY, Issue 7-8 2008
V. Sultana
Abstract Solvent fractions (i.e. n -hexane, chloroform and methanol) of the ethanol extracts of the seaweeds Codium iyengarii, Jania capillacea, Stokeyia indica and Solieria robusta caused more than 50% mortality of Meloidogyne javanica juveniles within 24 h at 10 mg/ml. Nematode mortality increased with an increase in fraction concentration or exposure time. The n -hexane fractions from S. indica, J. capillacea and C. iyengarii and the chloroform fraction from S. robusta also resulted in more than 50% mortality within 48 h at 1.0 mg/ml. In a screen-house experiment application of S. indica and S. robusta as soil amendments alone or with Pseudomonas aeruginosa, a plant growth promoting rhizobacterium (PGPR), significantly suppressed infection of chili roots by root-infecting fungi Macrophomina phaseolina, Rhizoctonia solani, Fusarium solani and the root knot nematode Meloidogyne javanica. Seaweed alone or with PGPR also increased plant growth. Suppressive effect on root pathogens and growth enhancement potential of seaweeds and P. aeruginosa were also effective in field plots. [source]

Identity and Pathogenicity of Fungi Associated with Root and Crown Rot of Soft Red Winter Wheat Grown on the Upper Coastal Plain Land Resource Area of Mississippi

JOURNAL OF PHYTOPATHOLOGY, Issue 2 2000
M. S. Gonzalez
Seedling stand, disease severity and fungal incidence were determined from untreated ,Wakefield' soft red winter wheat planted on a Leeper silty clay loam in field tests conducted at the Mississippi Agricultural and Forestry Experiment Station, Plant Science Research Center, Mississippi State University, Starkville, Mississippi during the 1996,97 and 1997,98 growing seasons. Seedling stand was reduced by 40% each year in plots established with untreated seed. Cochliobolus sativus was the most frequently isolated fungus. Fusarium acuminatum, Fusarium equiseti and Fusarium solani were the most prevalent Fusarium spp. Seven other Fusarium spp. and 23 species of other fungal genera were isolated. Pathogenicity tests with three isolates each of C. sativus, Cochliobolus spicifer, F. acuminatum, F. solani, F. equiseti, Fusarium compactum, Embellisia chlamydospora and Microdochium bolleyi were performed in test tube culture and two isolates each of C. sativus, C. spicifer, F. acuminatum, E. chlamydospora and M. bolleyi under greenhouse conditions. In test tubes and in the greenhouse, seedlings infected with isolates of C. sativus developed seedling blight, discoloration and necrosis, primarily in seminal roots and crowns. In the greenhouse, C. sativus induced lesions on the lower leaf sheath and reduced seedling height, seedling emergence, dry and fresh weight of roots and shoots. Isolates of F. acuminatum, F. solani, F. equiseti, F. compactum, E. chlamydospora and M. bolleyi induced slight to moderate orange to light-brown discoloration of crown and seminal roots in test tubes. Cochliobolus spicifer isolates had the most pre-emergence activity, inducing black root discoloration and root pruning of wheat seedlings and reducing seedling emergence, root fresh weight and shoot dry weight. In the greenhouse, F. acuminatum reduced seedling height, seedling emergence and root and shoot dry weights. Microdochium bolleyi and E. chlamydospora reduced fresh and dry weight of roots, plant emergence and shoot dry weight. Fusarium acuminatum and C. spicifer reduced the growth rate of wheat seedlings. All fungi evaluated showed increased disease severity compared to the untreated control. The high frequency of isolation of C. sativus from crown and root tissues can be partially explained by the dry, warm conditions during the early stages of wheat seedling development in the Upper Coastal Plain Land Resource Area of Mississippi. Zusammenfassung Die Auflaufrate von Sämlingen, die Stärke des Krank-heitsbefalls sowie die Häufigkeit von Pilzarten wurden bei nicht behandelten roten Weichwinterweizen der Sorte Wakefield ermittelt, welche in einem Leeper schlammigen Tonboden an der Mississippi Agricultural & Forestry Experiment Station, Plant Science Research Center, Mississippi State University, Starkville, Mississippi in der 1996,97 und 1997,98 Saison gesät worden waren. In beiden Jahren wurde die Auflaufrate von nicht behandeltem Saatgut um 40% reduziert. Cochliobolus sativus wurde am häufigsten isoliert. Fusarium acuminatum, Fusarium equiseti und Fusarium solani waren die überwiegenden Fusarium spp. Außierdem wurden sieben weitere Fusarium spp. sowie 23 weitere Pilzarten isoliert. Pathogenitätstests mit je 3 Isolaten von C. sativus, Cochliobolus spicifer, F. acuminatum, F. solani, F. equiseti, Fusarium compactum, Embellisia chlamydospora und Microdochiumbolleyi wurden in Reagenzröhrchen durchgeführt, sowie mit je 2 Isolaten von C. sativus, C. spicifer, F. acuminatum, E. chlamydospora und M. bolleyi unter Gewächshausbedingungen. Sowohl in den Reagenzröhrchen als auch im Gewächshaus entwickelten Sämlinge, die mit C. sativus inokuliert worden waren, eine Fäule, Verfärbung sowie Nekrosis, hauptsächlich in den sekundären Wurzeln und in den Halmbasen. Unter Gewächshausbedingungen verursachte C. sativus außierdem Läsionen der unteren Blattscheide sowie eine Reduzierung des Sämlingswachstums, des Sämlingsauflaufs, des Trocken-und Frischgewichts der Wurzeln und Sprossen. Im Reagenzröhrchentest induzierten Isolate von F. acuminatum, F. solani, F. equiseti, F. compactum, E. chlamydospora und M. bolleyieine schwache bis mäßiige orange bis hell braune Verfärbung des Halmbasis und der Sekundärwurzeln. Isolate von C. spicifer besaßien die höchste Vorauflaufaktivität und induzierten eine Verschwärzung und Verkürzung der Wurzeln sowie eine Reduzierung des Sämlingsauflaufs, des Wurzelfrischgewichts sowie des Sproitrockengewichts. Unter Gewächshausbedingungen reduzierte F. acuminatum die Sämlingshöhe, die Auflaufrate sowie das Trockengewicht der Wurzeln und Sproien. Microdochium bolleyi und E. chlamydospora reduzierten das Frisch-und Trockengewicht der Wurzeln, die Auflaufrate sowie das Sproßitrockengewicht. Die Wachstumsrate der Sämlinge wurde durch F. acuminatum und C. spicifer reduziert. Alle untersuchten Pilzarten erhöhten die Befallsstärke verglichen mit der unbehandelten Kontrolle. Die hohe Isolierungsrate von C. sativus aus dem Halmbasis-und Wurzelgewebe kann zum Teil dadurch erklärt werden, dass während der Frühentwicklungsphase der Sämlinge trockene und warme Wachstumsbedingungen in diesem Gebiet herrschten. [source]

Antifungal property of the essential oils and their constituents from Cinnamomum osmophloeum leaf against tree pathogenic fungi

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 12 2005
Han-Chung Lee
Abstract This study compares the chemical constituents of leaf essential oils from various geographical provenances of Cinnamomum osmophloeum and investigates their antifungal activities against six tree pathogenic fungi. According to GC-MS and cluster analyses, the leaf essential oils obtained from different geographical provenances and their relative contents were classified into six chemotypes: cinnamaldehyde type, cinnamaldehyde,cinnamyl acetate type, cinnamyl acetate type, linalool type, camphor type, and mixed type. Results from the antifungal tests show that the leaf essential oils of cinnamaldehyde type and cinnamaldehyde,cinnamyl acetate type have excellent inhibitory effect against Rhizoctonia solani, Collectotrichum gloeosporioides, Ganoderma australe and Fusarium solani. Furthermore, among the fourteen constituents of C osmophloeum leaf essential oils, Z -cinnamaldehyde, eugenol, geraniol and citral display the best antifungal properties. Comparisons of the antifungal properties of Z -cinnamaldehyde congeners reveal that Z -cinnamaldehyde exhibits the best antifungal property of this group. Copyright © 2005 Society of Chemical Industry [source]

In vitro and in vivo antifungal activity of cetrimide (cetyltrimethyl ammonium bromide) against fungal keratitis caused by Fusarium solani

MYCOSES, Issue 1 2007
Yehia A.-G.
Summary Mycotic keratitis is a devastating eye infection acquired after eye injury. Cetrimide at 15 and 20 mg ml,1 produced no surviving Fusarium solani growth with minimal inhibitory concentration value of 0.10 mg ml,1. Topical administration of three drops (0.3 ml) of cetrimide aqueous solution of 10 mg ml,1 at pH 6.4 three times daily succeeded to cure human severe resistant F. solani keratitis in a time course of <3 weeks, and with complete healing after 6 weeks. Cetrimide-treated rabbit corneas section appeared with normal compact epithelium and endothelium with no vacuolation in Descemet's endothelial complex: an indication that cetrimide has no significant toxic effects. So, cetrimide at 10 mg ml,1 may be effective and safe topical therapy in patients with mycotic keratitis, especially F. solani ulcers. Currently, there is no antimycotic drug with a good corneal penetration, which is safe and has a fungicidal activity. [source]

Do multipurpose contact lens disinfecting solutions work effectively against non-FDA/ISO recommended strains of bacteria and fungi?

OPHTHALMIC AND PHYSIOLOGICAL OPTICS, Issue 1 2010
Maureen Boost
Abstract Purpose:, Recent outbreaks of microbial keratitis have increased concerns about the efficacy of multipurpose solutions (MPS) against ,real-world' organisms. This study determined, in accordance with FDA/ISO standard methods, the effects of five MPS against clinical isolates and type strains of bacteria, and isolates of fungi from subjects' ocular structures; and of three MPS against environmental fungal isolates. Method:, MPS were challenged with bacteria (type strains (ATCC) and clinical isolates of bacterial pathogens obtained from a district hospital laboratory) and with fungal isolates from both the periocular and conjunctival structures and from environmental air. Results:, All MPS demonstrated at least a 3-log reduction of challenged cell viability of all bacterial species tested, with the exception of MPS D against a canine infection Staphylococcus aureus isolate. Whilst all MPS tested were able to effect a 1.0-log reduction of viability of Fusarium solani (ATCC 36031), only two MPS had 90% viability reduction against all fungi of human origin and only one of these against all environmental fungal isolates. Effectiveness of these two solutions against fungal isolates compared to the remaining three MPS was found to be statistically significant (p = 0.003). Conclusions:, All MPS demonstrated a 99.9% viability reduction against a wide range of bacteria including major ocular pathogens not currently included in the FDA panel. The inability of three MPS to achieve a 90% reduction against fungal isolates is of concern as there has been a recent upsurge in reports of fungal keratitis. We would recommend extension of the current FDA testing panel for MPS to include more fungal isolates. [source]

Root and stem rot of Schefflera arboricola in Brazil, caused by Fusarium solani

PLANT PATHOLOGY, Issue 6 2001
NEW DISEASE REPORT
No abstract is available for this article. [source]

Fungal community diversity and soil health in intensive potato cropping systems of the east Po valley, northern Italy

ANNALS OF APPLIED BIOLOGY, Issue 2 2009
L.M. Manici
Abstract An ecological approach was used to investigate the relationship between diversity of soil fungal communities and soil-borne pathogen inoculum in a potato growing area of northern Italy affected by yield decline. The study was performed in 14 sites with the same tillage management practices: 10 named ,potato sites', that for many years had been intensely cultivated with potatoes, and 4 named ,rotation sites', subject to a 4-year rotation without potatoes or any recurrent crop for many years. Fungal communities were recorded using conventional (soil fungi by plate count and endophytic fungi as infection frequency on pot-grown potato plant roots in soil samples) and molecular approaches [Basidiomycetes and Ascomycetes with specific and denaturing gradient gel electrophoresis (DGGE) analysis]. Diversity of fungal communities in potato sites was significantly lower than that in rotation sites. In addition, fungal communities in rotation sites showed lower Berger,Parker dominance than those in the potato sites, suggesting that rotation sites had a higher diversity as well as a better fungal community balance than potato sites. The ANalysis Of SIMilarity test of soil fungi and root endophytic fungi revealed that the two cropping systems differed significantly for species composition. Root endophytic fungal communities showed a greater ability to colonise potato roots in soil samples from potato sites than those from rotation sites. Moreover, the majority of endophytic root fungal community species in potato sites belonged to the potato root rot complex and storage disease (Colletotrichum coccodes, Fusarium solani and Fusarium oxysporum), while those in rotation sites were mainly ubiquitous or saprobic fungi. Soil rDNA analyses showed that Ascomycetes were much more frequent than Basidiomycetes in all the soils examined. DGGE analysis, with the Ascomycete-specific primer (ITS1F/ITS4A), did not reveal distinctions between the communities found at the potato and rotation sites, although the same analysis showed differences between the communities of Basidiomycetes (specific primer ITS1F/ITS4B). These findings showed that recurrent potato cropping affected diversity and composition of soil fungal communities and induced a shift in specialisation of the endophytic fungi towards potato. [source]

Synthesis of antibacterial and antifungal cobalt(II), copper(II), nickel(II) and zinc(II) complexes with bis-(1,1,-disubstituted ferrocenyl)thiocarbohydrazone and bis-(1,1,-disubstituted ferrocenyl)carbohydrazone

APPLIED ORGANOMETALLIC CHEMISTRY, Issue 7 2004
Zahid H. Chohan
Abstract The condensation reaction of 1,1,-diacetylferrocene with thiocarbohydrazide and carbohydrazide to form bis-(1,1,-disubstituted ferrocenyl)thiocarbohydrazone and bis-(1,1,-disubstituted ferrocenyl)carbohydrazone has been studied. The compounds obtained have been further used as ligands for their ligand and antimicrobial properties with cobalt(II), copper(II), nickel(II) and zinc(II) metal ions. The compounds synthesized have been characterized by physical, spectral and analytical methods and have been screened for antibacterial activity against Escherichia coli, Bacillus subtillis, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi, and for antifungal activity against Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani and Candida glaberata using the agar well-diffusion method. All the compounds synthesized have shown good affinity as antibacterial and antifungal agents, which increased in most of the cases on complexation with the metal ions. Copyright © 2004 John Wiley & Sons, Ltd. [source]

Comparative activity and stability under salinity conditions of different antimicrobial peptides isolated from aquatic animals

AQUACULTURE RESEARCH, Issue 16 2009
Sara Emelie Löfgren
Abstract This study reports the in vitro activity of six antimicrobial peptides (AMPs) produced by aquatic animals (most marine invertebrates): tachyplesin (Tach), magainin (Mag), clavanin (Clav), penaeidin (Pen), mytilin (Myt) and antilipopolysaccharide factor (ALF) against marine vibrios, filamentous fungi and yeast. Their stability under salinity conditions and seawater was also examined. The results showed that Mag, Myt and especially Tach and ALF (minimum inhibitory concentration<1.5 ,M) had a potent activity against all tested vibrio species, whereas Clav and Pen were ineffective (up to 50 ,M). With respect to the antifungal activity, each AMP had a different potency according to the fungal species. In general terms, Tach was the most potent peptide, followed by Mag. Interestingly, Tach, Myt and ALF had a significant effect on the filamentous fungus Fusarium solani that could be pathogenic to marine organisms. All AMPs had a tendency to decrease or lose their activity at high salinity (>225 mM NaCl). Tach and Myt were the most stable peptides, maintaining significant activity under seawater salinity (450 mM). Curiously, all peptides lost their effect under seawater conditions. The results suggest that Tach, ALF and Myt are the most promising candidates for potential therapeutic use in farmed-marine species, because all have a significant and broad antimicrobial activity maintained at high salinity. [source]

Molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested polymerase chain reaction based on 28S ribosomal RNA gene sequences

BRITISH JOURNAL OF DERMATOLOGY, Issue 5 2009
M. Ebihara
Summary Background, Onychomycosis is often caused by dermatophytes, but the role of nondermatophytes is underestimated due to the difficulty of identifying them by conventional direct microscopy and culture. Objectives, This study aims to detect nondermatophytes, as well as dermatophytes, in the nail samples of patients with onychomycosis using a polymerase chain reaction (PCR)-based culture-independent method. Materials and methods, The nested PCR assay targeting the sequence of the 28S ribosomal RNA gene was used to amplify fungal DNAs from 50 microscopy-positive nail specimens. Newly designed primer sets for dermatophyte universal, Trichophyton rubrum, T. mentagrophytes, Aspergillus spp., Scopulariopsis brevicaulis, Fusarium solani, F. oxysporum, F. verticillioides, Candida albicans and C. tropicalis were used after confirmation of their specificity. Results, Forty-seven cases (94%) were positive for fungal DNA, among which dermatophytes were detected in 39 cases (83·0%): T. rubrum in 35 cases (74·5%) and T. mentagrophytes in eight cases (17·0%). Surprisingly, nondermatophytes were detected in 18 cases (38·3%), both dermatophytes and nondermatophytes in 10 cases (21·3%) and nondermatophytes alone in eight cases (17·0%). Aspergillus spp. alone was observed in five cases (10·6%). Conclusions, This study indicates that most of the affected nail plates of patients with onychomycosis were positive for specific fungal DNAs, and suggests that nondermatophytes detected at high rates may be involved in the pathogenesis of onychomycosis. [source]

A case of melanonychia caused by Fusarium solani

BRITISH JOURNAL OF DERMATOLOGY, Issue 3 2002
H.J. Lee
No abstract is available for this article. [source]

Effect of voriconazole on a corneal abscess caused by fusarium

ACTA OPHTHALMOLOGICA, Issue 6 2004
Anna Polizzi
Abstract. Purpose:, To describe a case of corneal abscess caused by Fusarium solani that did not respond to common antifungal agents. Method:, Case report. Results:, Twenty days after accidental contact with vegetation, a 56-year-old man presented with a corneal abscess. Corneal ulceration developed and a perforating keratoplasty was performed. After a microbiological examination, the diagnosis of F. solani infection was made. Systemic and topical amphotericin B and fluconazole were prescribed, with no results. A new abscess formed on the transplanted graft and a wound leak developed. We administered topical and systemic voriconazole. No side-effects were observed. The choroidal detachment and the surgical transplant recovered completely in 20 days. A vascular leukoma developed at the site of the transplanted corneal abscess. Conclusion:, From a functional point of view, another corneal transplant will be necessary. Voriconazole was effective in treating a severe keratomycosis caused by F. solani that was resistant to other topical and systemic antifungal agents. [source]