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Force Analysis (force + analysis)
Selected AbstractsNSOM- and AFM-based nanotechnology elucidates nano-structural and atomic-force features of a Y. pestis V immunogen-containing particle vaccine capable of eliciting robust responsePROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 6 2009Gucheng Zeng Abstract It is postulated that unique nanoscale proteomic features of immunogen on vaccine particles may determine immunogen-packing density, stability, specificity, and pH-sensitivity on the vaccine particle surface and thus impact the vaccine-elicited immune responses. To test this presumption, we employed near-filed scanning optical microscopy (NSOM)- and atomic force microscopy (AFM)-based nanotechnology to study nano-structural and single-molecule force bases of Yersinia pestis (Y. pestis) V immunogen fused with protein anchor (V-PA) loaded on gram positive enhancer matrix (GEM) vaccine particles. Surprisingly, the single-molecule sensitive NSOM revealed that ,90% of V-PA immunogen molecules were packed as high-density nanoclusters on GEM particle. AFM-based single-molecule force analyses indicated a highly stable and specific binding between V-PA and GEM at the physiological pH. In contrast, this specific binding was mostly abrogated at the acidic pH equivalent to the biochemical pH in phagolysosomes of antigen-presenting-cells in which immunogen protein is processed for antigen presentation. Intranasal mucosal vaccination of mice with such immunogen loaded on vaccine particles elicited robust antigen-specific immune response. This study indicated that high-density, high-stability, specific, and immunological pH-responsive loading of immunogen nanoclusters on vaccine particles could readily be presented to the immune system for induction of strong antigen-specific immune responses. [source] The reproductive role hypothesis explains trophic morphology dimorphism in the northern map turtleFUNCTIONAL ECOLOGY, Issue 5 2008G. Bulté Summary 1Sexually dimorphic traits often reflect factors limiting the reproductive success of animals. Thus, most sexually dimorphic traits can be directly linked to the reproductive role of each sex. Sexual dimorphism in trophic structures (e.g. beak, jaws, teeth), however, often lacks a direct link to reproduction. 2Trophic structures can be linked indirectly to reproductive allocation via energy acquisition. The reproductive role hypothesis (also known as the dimorphic niche hypothesis) posits such an indirect link, but has received heretofore little direct empirical support. We tested this hypothesis in a molluscivorous turtle exhibiting marked female-biased trophic morphology dimorphism. 3Bite force analysis showed that females have stronger jaws than males and dietary analysis revealed that females ingest snails closer to their maximum biting capacity than males. Body condition of both sexes and reproductive output of females increased with relative head width, indicating that fitness is tightly linked to head size and bite force. 4Our study provides strong evidence that reproductive role contributes to sexual dimorphism in trophic morphology. Our findings should apply to any animal in which energy intake is limited by trophic morphology. [source] Optimizing Beef Chuck Flavor and Texture Through Cookery MethodsJOURNAL OF FOOD SCIENCE, Issue 4 2004K. Adhikari ABSTRACT: Eight cooked chuck muscles were subjected to Warner-Bratzler shear (WBS) force analysis in phase I of the study to choose the cooking methods and temperature combinations suitable for descriptive sensory analysis in phase II. WBS force analysis was unable to discriminate (P > 0.05) cooking and temperature combinations among various muscles. Nine chuck muscles were then subjected to cooking by grilling, roasting, and braising to an internal temperature of 63°C and 77°C. A panel of 10 trained judges performed the descriptive analysis of the cooked chuck muscle steaks. Grilling, under medium rare conditions, was most suitable for the chuck muscles because it yielded more juiciness and roasted flavor than any other cooking method/temperature combinations. [source] Three-dimensional force measurements on oral implants: a methodological studyJOURNAL OF ORAL REHABILITATION, Issue 9 2000J. Duyck This paper describes a methodology that allows in vitro and in vivo quantification and qualification of forces on oral implants. Strain gauges are adapted to the outer surface of 5·5 and 7 mm standard abutments (Brånemark System®, Nobel Biocare, Sweden). The readings of the strain gauges are transformed into a numerical representation of the normal force and the bending moment around the X- and Y- axis. The hardware and the software of the 3D measuring device based on the strain gauge technology is explained and its accuracy and reliability tested. The accuracy level for axial forces and bending moments is 9.72 N and 2.5 N·cm, respectively, based on the current techniques for strain gauged abutments. As an example, an in vivo force analysis was performed in a patient with a full fixed prosthesis in the mandible. Since axial loads of 450 N and bending moments of 70 N·cm were recorded, it was concluded that the accuracy of the device falls well within the scope of our needs. Nevertheless, more in vivo research is needed before well defined conclusions can be drawn and strategies developed to improve the biomechanics of oral implants. [source] | ||||||||||