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Fishmeal
Terms modified by Fishmeal Selected AbstractsN -3 enrichment of pork with fishmeal: Effects on production and consumer acceptabilityEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Issue 8 2008Stelios Sioutis Abstract This study aimed to develop pork products of acceptable organoleptic quality enriched with long-chain n -3 polyunsaturated fatty acids (LC n -3 PUFA). Female pigs were fed a finisher diet containing 15% PorcOmegaÔ tuna fishmeal or a commercial diet. Pigs and rations were weighed weekly. The feed conversion ratio was significantly lower in pigs fed fishmeal than in controls (2.61,±,0.01 versus 2.96,±,0.06, p,<0.05). After 6,weeks, the pigs were butchered and the fatty acid contents of selected pork products were analyzed by gas chromatography. Shelf life was tested by thiobarbituric acid-reactive substances (TBARS), initially and after 5,days of cold storage, and sensory qualities were evaluated after cooking. LC n -3 PUFA contents of pork products from pigs fed fishmeal were higher than in controls (steak 300%, stir-fry 250%, diced 520%, mince 480%, sausage 360%; p,<0.05 in all cases). There were no differences between n -3-enriched and regular pork in either TBARS content of stored raw products or sensory characteristics after cooking. Incorporating fishmeal in the finisher diet resulted in greater feed efficiency and production of nutritionally enhanced pork products with organoleptic profiles and stability comparable to those of regular pork. [source] Carp (Cyprinus carpio) oils obtained by fishmeal and ensilage processes: characteristics and lipid profilesINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 8 2009Valéria T. Crexi Summary Fish oil is an important source of long-chain ,-3 polyunsaturated fatty acid. The common carp (Cyprinus carp) is a major fish species in world aquaculture production. This study aimed towards obtaining carp viscera oil by ensilage and fishmeal processes. Characteristics of crude oils obtained were also compared with oil extracted by Bligh and Dyer methods. Crude oils obtained by the three processes resulted in significant difference (P < 0.05) for free fatty acids, peroxide, thiobarbituric acid and Lovibond colour values; however, iodine and saponification values were not significantly affected (P > 0.05). Recovery yield of crude oils was approximately 85% in relation to carp viscera oil. Carp crude oils obtained by the ensilage and fishmeal processes resulted in high unsaturated and polyunsaturated fatty acid contents (67.4%), and ,3/,6 ratios around 1.15. These oils are applicable in fish diets; however, crude oils require refinement for human consumption. [source] Growth performance and health status in weanling piglets fed spray-dried porcine plasma under typical Northern European conditionsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 1-2 2002A. J. VAN DIJK The effect of inclusion of spray-dried porcine plasma (SDPP) in diets for weanling piglets was studied. The objectives were to determine whether SDPP would have positive effects on post-weaning piglet performance and health under typical Northern European conditions. In experiment 1, 160 weanling piglets were assigned randomly to a control diet or a diet containing 3% SDPP, which was added at the expense of both fishmeal and dried skim milk. In experiment 2, 264 weanling piglets were assigned to a control diet containing whey protein, a diet without whey protein but with SDPP or a diet containing both whey protein and SDPP. In essence, SDPP was added to the test diets at the expense of either whey protein or fishmeal. Piglets were fed the diets for 3 weeks. In experiment 1, the piglets fed the SDPP diet had a 7% higher average daily gain (ADG) and a 4% lower feed conversion ratio (FCR) (p < 0.05) during the first 3 weeks after weaning than did those fed the control diet. There were no differences in leucocyte counts or ,-globulin. In experiment 2 there were no significant differences in ADG and FCR among the dietary treatments. It is concluded that low amounts of SDPP in weanling diets can have positive effects on growth performance under Northern European conditions. [source] Influence of dietary amino acid profiles on growth performance and body composition of juvenile grouper Epinephelus coioidesJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2 2008Z. Luo Summary A feeding experiment was conducted to investigate the effects of dietary amino acid (AA) profiles on growth performance and body composition of juvenile grouper Epinephelus coioides (initial mean weight: 68.1 ± 1.0 g, mean ± SD). Five diets contained 30% fishmeal, 12% soy protein concentrate and 20% crystalline amino acids (CAAs); the control diet contained 54% fishmeal and 17% soy protein concentrate as intact protein sources. CAAs were added to the five diets to simulate the AA pattern found in white fishmeal protein (WFP), brown fishmeal protein (BFP), hen egg protein (HEP), grouper E. coioides juvenile protein (GJP) and red sea bream egg protein (REP), respectively. The highest WG and SGR were obtained in fish fed the control diet, followed by fish fed the diets with AA profiles of WFP and GJP. Fish fed the diets with AA profiles of BFP, REP and HEP showed relatively poor growth performance. Feed utilization showed a similar trend in growth parameters. Protein content of whole body among these treatments showed no significant differences (P > 0.05), but lipid content of whole body showed the highest value in the control group (P < 0.05). Dietary AA profiles significantly influenced plasma protein, cholesterol, triacylglycerol and glucose concentrations (P < 0.05). Dietary AA profiles significantly influenced the condition factor, hepatosomatic index and intraperitoneal fat ratio (P < 0.05). [source] Nutritional evaluation of fermented black gram (Phaseolus mungo) seed meal in compound diets for rohu, Labeo rohita (Hamilton), fingerlingsJOURNAL OF APPLIED ICHTHYOLOGY, Issue 1 2007S. Ramachandran Summary Six isonitrogenous (approximately 35% crude protein) and isocaloric (approximately 4.0 kcal g,1) diets were formulated incorporating raw and fermented black gram, Phaseolus mungo, seed meal at 20%, 30% and 40% levels by weight into a fishmeal-based control diet fed to rohu, Labeo rohita, fingerlings (mean weight, 1.81 ± 0.21 g) for 80 days for a study of fish performance. A particular bacterial strain (Bacillus sp.) isolated from the intestine of adult common carp (Cyprinus carpio) reared in the wild having significant amylolytic, cellulolytic, lipolytic and proteolytic activities was used for fermentation of seed meal for 15 days at 37 ± 2°C. Fermentation of P. mungo seed meal was effective in significantly reducing the crude fibre content and antinutritional factors such as tannins and phytic acid, and enhancing available free amino acids and fatty acids. In terms of growth, feed conversion ratio and protein efficiency ratio, the 30% fermented black gram seed meal incorporated diet resulted in a significantly (P < 0.05) better performance of rohu fingerlings. In general, growth and feed utilization efficiencies of diets containing fermented seed meal were superior to diets containing raw seed meal. The apparent protein digestibility (APD) values decreased with increasing levels of raw seed meal in the diets. The APD for raw seed meal was lower at all levels of inclusion in comparison to those for the fermented seed meals. The maximum deposition of protein in the carcass was recorded in fish fed the diet containing 40% fermented seed meal. The results indicate that fermented black gram seed meal can be incorporated in carp diets up to the 30% level compared to the 10% level of raw seed meal. [source] Effect of inclusion of blue-green algae meal on growth and accumulation of microcystins in gibel carp (Carassius auratus gibelio)JOURNAL OF APPLIED ICHTHYOLOGY, Issue 1 2006M. Zhao Summary Six isonitrogenous (crude protein content: 38%) and isoenergetic (gross energy content: 17 kJ g,1) diets were formulated to investigate the effects of inclusion of blue-green algae meal on gibel carp (Carassius auratus gibelio). In each diet, 15% of the protein was supplied by fishmeal; the remainder was supplied by soybean meal and blue-green algae meal. Diet 1 was used as control with no blue-green algae meal whereas the content in diets 2,6 was 15.15, 29.79, 44.69, 59.58 and 74.48%, respectively. Each diet was fed to five groups of gibel carp for 12 weeks in a flow-through system. Final body weight and specific growth rate (SGR) of fish fed diet 5 were significantly lower than the control diet (P < 0.05). Mortality of gibel carp increased with increase in algae meal inclusion (P < 0.05), but there was no significant difference between fish fed diets 3,6 (P > 0.05). Feed conversion efficiency (FCE) decreased with the increase in algae meal inclusion (P < 0.05). Fish-fed diet 6 showed the highest feeding rate (P < 0.05), while there were no significant differences among the other groups (P > 0.05). Apparent digestibility coefficient of dry matter, protein, and energy decreased with increasing algae meal inclusion in the diets (P < 0.05). Aspartate aminotransferase (GOT) activity in the liver was not significantly different among groups (P > 0.05). Liver alanine aminotransferase (GPT) activity of fish-fed diets 4, 5 and 6 was significantly lower than the control diet (diet 1; P < 0.05). Microcystins in the muscle, liver, gallbladder, and spleen increased with increasing algae inclusion (P < 0.05). [source] Growth and product quality of European eel (Anguilla anguilla) as affected by dietary protein and lipid sourcesJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2 2003U. Luzzana Summary A 12-week feeding trial was undertaken to evaluate the effects of partial replacement of marine raw materials in the diet with soybean meal and beef tallow on growth and product quality of European eel (Anguilla anguilla). Based on growth, feed efficiency and chemical composition, soybean meal was found to be an unsuitable ingredient for eel diets as a substitute for fishmeal (25% on a protein basis), probably because of the low digestibility of its carbohydrate content. However, beef tallow can be used to replace 50% of fish oil without reduction in growth, provided that digestible carbohydrates are present in the diet. No major effects of protein and lipid sources in the diet were found on fillet chemical composition. Sensory analysis revealed no significant differences between fish fed the control and the experimental diets, with the exception of salty taste which was significantly higher in fish fed combined soybean meal and beef tallow compared with fish fed the control diet. [source] Soybean meal alters autochthonous microbial populations, microvilli morphology and compromises intestinal enterocyte integrity of rainbow trout, Oncorhynchus mykiss (Walbaum)JOURNAL OF FISH DISEASES, Issue 9 2009D L Merrifield Abstract Rainbow trout were fed either a diet containing fishmeal (FM) as the crude protein source or a diet containing 50% replacement with soybean meal (SBM) for 16 weeks. An enteritis-like effect was observed in the SBM group; villi, enterocytes and microvilli were noticeably damaged compared with the FM group. The posterior intestine microvilli of SBM-fed fish were significantly shorter and the anterior intestine microvilli significantly less dense than the FM-fed fish. Electron microscopy confirmed the presence of autochthonous bacterial populations associated with microvilli of both fish groups. Reduced density of microvilli consequently led to increased exposure of enterocyte tight junctions, which combined with necrotic enterocytes is likely to diminish the protective barrier of the intestinal epithelium. No significant differences in total viable counts of culturable microbial populations were found between the groups in any of the intestinal regions. A total of 1500 isolates were tentatively placed into groups or genera, according to standard methods. Subsequent partial 16S rRNA sequencing revealed species that have not been identified from the rainbow trout intestine previously. Compared with the FM group levels of Psychrobacter spp. and yeast were considerably higher in the SBM group; a reduction of Aeromonas spp. was also observed. [source] Changes in immune and enzyme histochemical phenotypes of cells in the intestinal mucosa of Atlantic salmon, Salmo salar L., with soybean meal-induced enteritisJOURNAL OF FISH DISEASES, Issue 2 2000A M Bakke-McKellep Extracted soybean meal (SBM) in the diet for Atlantic salmon, Salmo salar L., causes an inflammatory response in the distal intestine. The morphological changes of the epithelial cells and a characterization of the inflammatory cell infiltrate of the distal intestinal mucosa were studied using a panel of enzyme and immunohistochemical markers. The salmon (average body weight 927 g) used in the study were fed either a fishmeal-based diet (control diet) or a diet in which 30% of the fishmeal protein was replaced with SBM protein (SBM diet). In salmon fed SBM, there were markedly reduced enzyme reactivities in the distal intestinal epithelial cells, both in the brush border [5,-nucleotidase (5,N), Mg2+-ATPase, alkaline phosphatase (ALP) and leucine aminopeptidase (LAP)] and in the intracellular structures [alkaline and acid phosphatase, non-specific esterase (NSE) and alanine aminopeptidase (AAP)]. There appeared to be an increased presence of cells of monocytic lineage, including macrophages, as well as neutrophilic granulocytes and immunoglobulin (Ig) M in the lamina propria of the SBM-fed fish. The mid intestine showed little response to the diet. The results suggest that toxic/antigenic component(s) of SBM affect the differentiation of the distal intestinal epithelial cells and may help explain the reduced nutrient digestibilities previously reported in salmonids fed extracted SBM. [source] Occupational allergy and asthma among salt water fish processing workersAMERICAN JOURNAL OF INDUSTRIAL MEDICINE, Issue 12 2008Mohamed F. Jeebhay MBChB Abstract Background Fish processing is a common economic activity in Southern Africa. The aim of this study was to determine the prevalence and host determinants of allergic symptoms, allergic sensitization, bronchial hyper-responsiveness and asthma among workers processing saltwater fish. Methods A cross-sectional study was conducted on 594 currently employed workers in two processing plants involved in pilchard canning and fishmeal processing. A modified European Community Respiratory Health Survey (ECRHS) questionnaire was used. Skin prick tests (SPT) used extracts of common airborne allergens, fresh fish (pilchard, anchovy, maasbanker, mackerel, red eye) and fishmeal. Spirometry and methacholine challenge tests (MCTs; tidal breathing method) used ATS guidelines. Results Work-related ocular-nasal symptoms (26%) were more common than asthma symptoms (16%). The prevalence of atopy was 36%, while 7% were sensitized to fish species and 26% had NSBH (PC20,,,8 mg/ml or ,12% increase in FEV1 post-bronchodilator). The prevalence of probable occupational asthma was 1.8% and fish allergic rhino-conjunctivitis 2.6%. Women were more likely to report work-related asthma symptoms (OR,=,1.94) and have NSBH (OR,=,3.09), while men were more likely to be sensitized to fish (OR,=,2.06) and have airway obstruction (OR,=,4.17). Atopy (OR,=,3.16) and current smoking (OR,=,2.37), but not habitual seafood consumption were associated with sensitization to fish. Conclusions Based on comparison with previous published studies, the prevalence of occupational asthma to salt water fish is lower than due to shellfish. The gendered distribution of work and exposures in fish processing operations together with atopy and cigarette smoking are important determinants of occupational allergy and asthma. Am. J. Ind. Med. 51:899,910, 2008. © 2008 Wiley-Liss, Inc. [source] Demonstration of salmon farming as a net producer of fish protein and oilAQUACULTURE NUTRITION, Issue 4 2010V.O. CRAMPTON Abstract To date aquaculture's reliance on dietary marine sources has been calculated on a fish weight-to-weight basis without considering the absolute amounts of nutrients but this approach neglects the often considerable differences in the nutritional value of fish. We propose simple nutrient-to-nutrient-based dependency measures that take into account these nutritional differences. In the first study reported here, individually tagged Atlantic salmon (Salmo salar) were reared in seawater supplied tanks with feed collection facilities. In the second, commercial net pens were used to grow over 200 000 fish. For both studies, a low marine ingredient feed containing approximately 165 g kg,1 fishmeal was compared to a control feed (approx 300 g kg,1 fishmeal) whilst fish oil inclusion was less markedly reduced. The low marine feeds supported similar growth and feed efficiency compared to the control feeds. With the low marine ingredient feeds, the weight of salmon protein and lipid produced through growth exceeded the weight of marine protein and lipid consumed by the fish meaning that salmon farming can be a net producer of fish protein and oil. The amount of n-3 long-chain polyunsaturated fatty acids deposited was sufficient to meet current recommendations from human health organizations. [source] Partial or total replacement of fish meal by local agricultural by-products in diets of juvenile African catfish (Clarias gariepinus): growth performance, feed efficiency and digestibilityAQUACULTURE NUTRITION, Issue 3 2010L. NYINA-WAMWIZA Abstract The study was undertaken to evaluate the growth performance and feed utilization of African catfish, Clarias gariepinus, fed six diets (D) in which fishmeal (FM) was gradually replaced by a mixture of local plant by-products. In diets 1 and 2, FM (250 g kg,1) was replaced by sunflower oil cake (SFOC). In diets 3 and 4, FM (250 and 150 g kg,1, respectively) was replaced by SFOC and bean meal (BM) while FM was totally substituted by a mixture of groundnut oil cake (GOC), BM and SFOC in diets 5 and 6. Sunflower oil cake was cooked, soaked or dehulled in order to determine the appropriate processing techniques for improving the SFOC nutritive value and to evaluate the apparent digestibility coefficient (ADC) values of the alternative diets. No significant differences were observed for daily feed intake, weight gain, specific growth rate (SGR) and feed efficiency (FE) among fish fed D1, D2, D3 (250 g kg,1 FM), D4 (150 g kg,1 FM) and D6 (0 g kg,1 FM). The highest SGR (3.2% per day) and FE (1.2) were achieved in fish fed D3, and the lowest in fish fed D5 (0% FM), suggesting a maximum acceptable dietary concentration of hulled SFOC below 250 g kg,1 in African catfish juveniles. Protein efficiency ratio ranged from 2.2 to 3.2 for all dietary treatments and was positively influenced by FM inclusion. African catfish were able to digest plant protein very efficiently in all diets tested. ADC of protein ranged from 88.6 to 89.5%, while ADC of energy was relatively low for diets containing hulled sunflower oilcake (71,74%) and high when sunflower oilcake was dehulled (78.6,81.3%). Similarly, ADC of dry matter was higher when sunflower was dehulled (72.1%) when compared with crude SFOC (60.5%). Soaking increased ADC values for neutral detergent fibre (NDF), dry matter, energy, protein and amino acids (AA). There were no significant differences in protein ADCs (88,90%) with increased levels of dietary vegetable ingredients. Both soaking and dehulling of sunflower before incorporation helped in the reduction of NDF, antitrypsin and tannins. Digestibility of all AA was generally high, greater than 90% for both indispensable and non-indispensable AA. Based on the data obtained, it was possible to totally replace menhaden fish meal with a mixture of vegetable proteins (72% of total dietary protein) when diets contained a relatively low percentage of animal protein (28% based on blood meal and chicken viscera meal) without negative effects. [source] Predicting the optimal dietary essential amino acid profile for growth of juvenile yellow perch with whole body amino acid concentrationsAQUACULTURE NUTRITION, Issue 3 2010S.D. HART Abstract Rapid methods of estimating dietary essential amino acid (EAA) requirements might facilitate increases in aquaculture production, particularly for new or emerging industries. We conducted a 12-week feeding study to test the hypothesis that whole body EAA concentrations and the quantified methionine requirement could be used to predict the remaining dietary EAA requirements for juvenile all-female yellow perch. Six purified diets were developed and fed to triplicate groups of fish for 12 weeks. The diets contained the EAA profile of fishmeal (FM), the profile as predicted by whole body analysis, the quantified methionine requirement and resulting A/E ratios (PRED), PRED + 20% of all EAA (PRED20), PRED + 40% of all EAA (PRED40), PRED + 20% threonine, isoleucine and tryptophan (PRED320), and PRED + 40% threonine, isoleucine and typtophan (PRED340). Mean weight gain and feed consumption were significantly higher in fish fed PRED20 (35.7 ± 3.2 and 55.0 ± 5.3 g, respectively) than in fish fed FSM (25.1 ± 0.4 g wt gain, 41.0 ± 1.9 g cons), PRED (23.4 ± 2.3 g wt gain, 40.1 ± 4.2 g cons) and PRED340 (22.9 ± 3.3 g wt gain, 35.0 ± 3.8 g cons). There was no significant difference in feed efficiency among treatments. We recommend an EAA profile similar to PRED20 for feeding all-female juvenile yellow perch. [source] Growth, nitrogen gain and indispensable amino acid retention of pacu (Piaractus mesopotamicus, Holmberg 1887) fed different brewers yeast (Saccharomyces cerevisiae) levelsAQUACULTURE NUTRITION, Issue 3 2010R.O.A. OZÓRIO Abstract A feeding-and-digestibility trials were carried out to evaluate the efficacy of replacing fishmeal with brewers yeast Saccharomyces cerevisiae in diets of pacu, Piaractus mesopotamicus, juveniles. The feeding trial was conducted during 54 days with 450 fish (26.6 ± 1.7 g) testing six isonitrogenous (270 g kg,1 crude protein) and isoenergetic (19 MJ kg,1 crude energy) diets, with increasing yeast level to replace 0 (control), 30, 35, 50, 70 or 100% of dietary fishmeal. Growth performance and feed utilization increased with increasing dietary yeast level until 50% fishmeal replacement. Protein retention efficiency was higher in fish fed 35 and 50%. Protein digestibility and the fillet hue (the red/green chromaticity) were not significantly different among all treatments. Nitrogen gains were significantly improved in fish fed 35% replacement diet compared to fish fed the control diet. The retentions of indispensable amino acids tended to increase with increasing dietary yeast levels, with maximum retention at 35,50%. On the basis of our results, replacing 50% fishmeal by yeast in pacu diets successfully improved feed efficiency and growth performance, and reduced nitrogen losses, thereby reducing the nitrogen outputs from fish farms. [source] Nutrients apparent digestibility coefficients of selected protein sources for juvenile Siberian sturgeon (Acipenser baerii Brandt), compared by two chromic oxide analyses methodsAQUACULTURE NUTRITION, Issue 6 2009H. LIU Abstract Apparent digestibility coefficients (ADCs) of dry matter (ADCd), crude protein (ADCp), energy (ADCe) and amino acids in selected feedstuffs were determined for juvenile Siberian sturgeon (8.38 ± 0.20 g). The tested feedstuffs were fishmeal (FM), meat and bone meal (MBM), poultry by-product meal, hydrolysed feather meal, fermented feather meal solvent-extracted cottonseed meal and soybean meal. ADCs were determined using a reference diet and test diets at 7 : 3 ratios with 5 g kg,1 chromic oxide (Cr2O3) as an inert marker. Fish were reared in a recirculating system and fed to apparent satiation five times daily. Cr2O3 in diets and faeces samples were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and acid-digestion colorimetry (AC) methods, respectively. The results showed that ICP-AES method was more accurate for Cr2O3 determination than AC method, and the results determined by ICP-AES method were used in this study. ADCd and ADCp of seven tested ingredients were lowest for MBM (59.1 and 84.5%) and highest for FM (79.9 and 94.5%); ADCe of tested ingredients were from 71.8% for SECM to 93.2% for FM. ADCs of amino acid in test ingredients followed similar trend to the ADCp. The ADCs of individual amino acids varied from 61.6% (histidine in MBM) to 98.8% (valine in FM). [source] Variation in commercial sources of soybean meal influences the severity of enteritis in Atlantic salmon (Salmo salar L.)AQUACULTURE NUTRITION, Issue 5 2009P.A. URÁN Abstract Soybean meal (SBM) is a potential alternative for the replacement of fishmeal in aquafeeds. In Atlantic salmon, however, dietary SBM causes an inflammation of the distal intestine, known as SBM-induced enteritis. The objective of the present study is to verify whether different (geographically spread) commercial sources of SBM yield contrasting inflammatory responses. To do so, six SBM batches from different origins were included in the Atlantic salmon diets at the level of 20%. After 4 weeks of feeding, the distal intestine of the salmon was sampled and scored by a semi-quantitative scoring system, which assessed six separated parameters, characterizing the extent of enteritis. The overall mean score as well as the score of the separate parameters varied between the different commercial sources of SBM included in the diet. The variation in SBM caused different degrees of disparity in the score of the separate parameters. The parameter that was most affected by the variation in the source of SBM was the disappearance of supranuclear vacuoles in enterocytes. In contrast, the increase in goblet cells showed the smallest variation between the different SBM sources. This study shows that different commercial sources of SBM can result in differences in the severity of SBM-induced enteritis in Atlantic salmon. [source] Distal intestinal gene expression in Atlantic salmon (Salmo salar L.) fed genetically modified maizeAQUACULTURE NUTRITION, Issue 1 2009M.K. FRØYSTAD-SAUGEN Abstract In the current experiment, RNA was isolated from the distal intestine (DI) of Atlantic salmon-fed fishmeal-based diets containing either genetically modified (GM) maize (Bt maize, Mon810®, Monsanto Company, St. Louis, Missouri, USA) or its conventional near-isogenic parental line (non-GM) for 82 days, both at 300 g kg,1 inclusion. From a suppression subtractive hybridization (SSH) cDNA library, 192 clones with similarity to both known and novel Atlantic salmon sequences were identified. Real-time PCR was used to study the differential expression of 10 clones between the dietary groups. Expression of a clone showing high protein similarity to proton-dependent high-affinity oligopeptide transporter was significantly upregulated in fish-fed GM maize compared with fish-fed non-GM maize. No significant differences in expression were observed for the nine other clones showing similarity to the following proteins: heat shock protein 90B, procathepsin B, interferon gamma-inducible protein 30, ferritin heavy subunit, serum lectin isoform/C-type mannose-binding lectin, fatty acid-binding protein/gastrotropin, ATP synthase [H+ transporting, mitochondrial F0 complex, subunit c (ATPSYNT)], sonic hedgehog and translationally controlled tumour protein. In conclusion, only minor differences in DI transcriptional gene expression was observed between fish fed the GM and non-GM maize diets. [source] Hyperaccumulation of selenium in hybrid striped bass: a functional food for aquaculture?AQUACULTURE NUTRITION, Issue 3 2008P.A. COTTER Abstract One method of increasing the value of aquacultured product is to produce fillets that are fortified with minerals that are beneficial to human health , that is enhance the functionality of an already healthy product. A good candidate mineral in this regard is selenium (Se) which is of vital importance to normal metabolism in humans. In order to evaluate the dose response and tissue accumulation of supplemental dietary Se, a study was undertaken with hybrid striped bass (HSB). Animals were fed diets supplemented with either organic (0,3.2 mg kg,1 as SelPlex®) or inorganic (0.2 and 0.4 mg kg,1 as sodium selenite) Se for 6 weeks. Because basal fishmeal-based diets contained 1.22 mg Se kg,1, doses of Se delivered equated to 1.22,4.42 mg kg,1. At trial end, greatest weight gain was observed in fish receiving 0.2 mg Se kg,1, irrespective of form (organic/inorganic). Se accumulation in HSB liver and fillet revealed a classical dose-response once a threshold level of 0.2 mg Se kg,1 was surpassed. Greatest tissue accumulation of Se was observed in fish fed the 3.2 mg Se kg,1 level (P > 0.0001). A 100 g portion of Se-enhanced HSB fillet would contain between 33 and 109 ,g Se, amounting to a dietary intake of between 25 and 80 ,g Se; a level that would satisfy present daily intake recommendations. Comparison of tissue Se levels indicated that the muscle provides a more conspicuous gauge of dietary Se dose-response than does liver. Dietary treatments of between 0.4 and 1.6 mg organic Se kg,1 reduced (P < 0.024) plasma glutathione peroxidase (GSH-Px) activity. No differences were observed in ceruloplasmin, lysozyme or GSH-Px activities between organic and inorganic Se when delivered at the 0.2 mg Se kg,1 level. Ceruloplasmin, lysozyme and GSH-Px levels were elevated (P , 0.025) in fish fed the diet containing 0.4 mg inorganic Se kg,1. [source] Partial or total replacement of fishmeal by solvent-extracted cottonseed meal in diets for juvenile rainbow trout (Oncorhynchus mykiss)AQUACULTURE NUTRITION, Issue 6 2006L. LUO Abstract The effect of solvent-extracted cottonseed meal (SCSM) as a partial or total replacement of fishmeal was studied in juvenile rainbow trout (Oncorhynchus mykiss). Six experimental diets SCSM0, SCSM25, SCSM50, SCSM75, SCSM75A and SCSMT, containing a gradient of SCSM 0, 152, 305, 465, 460 and 610 g kg,1 to replace 0, 112.5, 225, 337.5, 337.5 and 450 g kg,1 fishmeal protein were fed to triplicate groups (initial body weight of 39.2 ± 0.1 g) for 8 weeks. The diet SCSM75A was supplemented with lysine and methionine, to be similar to SCSM0 for juvenile rainbow trout. Faeces were colleted after 4 weeks of normal feeding for apparent digestibility coefficients (ADC) of dry matter, crude protein and gross energy determination. Total replacement of fishmeal adversely affected growth performance. Fish fed with diet SCSMT had significantly (P < 0.05) lower weight gain, specific growth ratio, feed conversion efficiency (FCE) and protein efficiency ratio than fish fed with other diets. The FCE of SCSM75 and SCSM75A were significantly lower (P < 0.05) than those of fish fed with SCSM0 diets. The ADC of the dry matter of SCSM75 and SCSMT were significantly lower than the SCSM0 diet, and the ADC of crude protein and the energy of SCSMT were the lowest (P < 0.05). The ADC of threonine, proline, alanine, valine, isoleucine, leucine, lysine and methionine of fish fed with diet SCSMT were lower. Lysine and methionine supplement positively affected the ADC of SCS75A diet. There were no significant differences in the fish body composition. It is shown that SCSM can be utilized in the juvenile rainbow trout diet up to 305 g kg,1, to replace about 50% of fishmeal protein in this experiment. [source] Water immersion time reduces the preference of juvenile tropical spiny lobster Panulirus ornatus for pelleted dry feeds and fresh musselAQUACULTURE NUTRITION, Issue 6 2005K.C. WILLIAMS Abstract Development of a pelleted dry feed as an alternative to feeding fresh fishery by-catch is an environmental priority for tropical spiny lobster aquaculture. Earlier studies have shown the lobster's acceptance of pelleted dry feed diminishes rapidly after immersion in water. In this work, we quantified the rate at which dry matter, total protein, soluble protein and individual and total free amino acids were lost from pieces of green-lipped mussel Perna canaliculus, a commercially-extruded Penaeus japonicus (kuruma) shrimp feed (KSF) and four laboratory-made, fishmeal-based, pelleted feeds upon immersion for up to 7.5 h. The laboratory-made feeds contained homogenates of either green-lipped mussel, polychaete (Marphysa sanguinea), prawn (Metapenaeus bennettae) or squid (Sepioteuthis spp.). After being immersed in water for 0, 2.5 or 5 h, these same feeds were offered as a paired choice with KSF in two preference feeding studies with juvenile Panulirus ornatus lobsters. The loobster's preference for fresh mussel always exceeded that of KSF, irrespective of immersion time. Regression of the proportional intake of test feeds against the relative leach rate of KSF identified soluble protein, glycine and taurine as the principal leachate components having the highest positive correlations with the lobster's feeding preference. [source] Partial replacement of fishmeal by soybean meal in diets for juvenile cobia (Rachycentron canadum)AQUACULTURE NUTRITION, Issue 3 2005Q.-C. ZHOU Abstract An 8-week feeding experiment was conducted in floating cages (1.5 × 1.0 × 2.0 m) to determine the potential use of defatted soybean meal (roasted and solvent-extracted) as a partial replacement of fishmeal in the isonitrogenous (approximately 450 g kg,1 CP [crude protein]) diet for juvenile cobia with an initial average weight of about 8.3 g. Diets were formulated to include 0, 100, 200, 300, 400, 500 and 600 g kg,1 (diets D0, D10, D20, D30, D40, D50 and D60, respectively) of fishmeal protein being substituted by defatted soybean meal without methionine supplementation. The results showed that weight gain rate decreased significantly when the replacement level of fishmeal protein was increased from 400 g kg,1 to 500 g kg,1, and the D60 diet was the lowest in all groups. These results indicate that up to 400 g kg,1 of fishmeal protein can be replaced by defatted soybean meal without causing significant reduction in growth. Feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly affected by the replacement level of fishmeal protein being substituted by defatted soybean meal, when the replacement level of fishmeal protein was 200 g kg,1 (diet, D20), FCR was the lowest and PER was the highest. There were no significant differences in the moisture, lipid, crude protein and ash content in whole body and muscle, while lipid content in liver increased as the dietary soybean meal replacement levels increased. There were significant differences in haemoglobin, haematocrit, red blood cell, plasma glucose and triglyceride concentration in fish fed diets with different soybean meal replacement levels. Results of this trial indicated that the optimum level of fishmeal protein replacement with defatted soybean meal, determined by quadratic regression analysis was 189.2 g kg,1, on the basis of maximum weight gain. [source] Evaluation of canola oils as alternative lipid resources in diets for juvenile red seabream, Pagrus auratusAQUACULTURE NUTRITION, Issue 5 2003B. Glencross Abstract This study examined three potential oil resources, crude and refined canola oil and refined soybean oil as replacements for added dietary fish oil in diets for juvenile red seabream. These oil resources were evaluated for their potential to replace added fish oil (40 g kg,1) in fishmeal based (600 g kg,1) diets, with 100 g kg,1 of total lipids. Each of the three plant oils was used to replace 25%, 50%, 75% or 100% of the added dietary fish oil. Each of the three plant oils showed potential as a replacement for dietary fish oil, although a significant reduction in growth and feed utilisation was observed with the complete (100%) replacement of added fish oil by crude canola oil. No other significant effects of oil type or inclusion level on growth were apparent. A negative control (no added fish oil or plant oil, 60 g kg,1 of total lipid) yielded poorer growth than all treatments except the diet containing 40 g kg,1 of added crude canola oil (100% replacement). This observation confirmed that the added oils were utilized by the fish. A positive control diet containing 80 g kg,1 of added fish oil (140 g kg,1 total dietary lipid) sustained the best growth in the study, confirming that the 13 experimental diets were energy limiting as planned. Notably, few effects of the alternative oils were seen on the proximate composition of the fish. However, the influence of the alternative oils on the tissue fatty acid composition was considerable, irrespective of plant oil type or processing grade. Particularly notable was the overall increase in the level of polyunsaturated fatty acids in the tissues of the fish fed the plant oil diets, with these trends becoming more apparent with the greater levels of fish oil replacement. Minimal reductions in the levels of the long-chain polyunsaturated fatty acids of eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acid were observed from any of the plant oil treatments. Sensory assessment, by an Australian taste panel, of the fish fed the fish oil reference, or the 100% replacement by refined canola or refined soybean diets showed a preference in order of canola oil > soybean oil > fish oil fed fish. Clearly, both canola and soybean oils have considerable potential as replacements of fish oils in diets for this species. [source] Evaluation of carbohydrate rich diets through common carp culture in manured tanksAQUACULTURE NUTRITION, Issue 3 2002P. KESHAVANATH Four diets (T0,T3) were formulated reducing the fishmeal (Indian) component by 100 g kg,1 from 300 to 0 g kg,1 and including proportionately increasing quantities of maize. Diets were fed for 120 days at 50 g kg,1 body weight to triplicate groups of common carp (av. wt. 2.11,2.18 g) stocked at 1 m,2 in mud bottomed cement tanks (18 m2), fertilized with poultry manure. Fish growth, SGR and FCR in the different treatments were statistically not significantly different (P > 0.05). PER was lowest for the 300 g fishmeal kg,1 diet treatment (diet T0), increasing with decrease in dietary fishmeal content (diets T1,T3). Fish survival ranged from 96.29 to 100%. Diets influenced carcass composition and digestive enzyme activity. A significant increase in lipid deposition was recorded with increasing dietary carbohydrate content. Amylase, protease and lipase activities were higher in fish fed with diets T2 and T3. The protein sparing effect of dietary carbohydrate and the economic implication of eliminating fishmeal from the diet are discussed. [source] Hypervitaminosis A in first-feeding fry of the Atlantic salmon (Salmo salar L.)AQUACULTURE NUTRITION, Issue 1 2002R. ØRNSRUD Atlantic salmon fry were reared on a fishmeal based diet with increasing levels of vitamin A (VA) (6, 122 and 938 mg retinol kg,1 dry feed) from startfeeding and for 14 weeks. Signs of VA stress, such as reduced fat stores, liver size and growth, were found for groups receiving 122 and 938 mg retinol kg,1. Signs of vitamin A toxicity, such as increased mortality, abnormal vertebral growth, and reduced growth, were found for groups receiving 938 mg retinol kg,1. These results suggest that excess VA in the early life stages of Atlantic salmon is deleterious for normal development. [source] Feeding Atlantic salmon Salmo salar L. soybean products: effects on disease resistance (furunculosis), and lysozyme and IgM levels in the intestinal mucosaAQUACULTURE NUTRITION, Issue 2 2000Krogdahl Two trials were initiated to investigate the consequences of including various soybean products in diets for Atlantic salmon Salmo salar L. on (1) mortality following infection by Aeromonas salmonicida ssp. salmonicida during a cohabitation challenge, and (2) the lysozyme and IgM content of the intestinal mucosa. Groups of salmon were fed control diets containing fishmeal as the sole protein source (Contr1 and Contr2, respectively), soy concentrate-containing diets (SoyConc1 and SoyConc2, respectively), or diets containing either solvent-extracted soybean meal (SoyMeal, trial 1) or soybean molasses (SoyMol, trial 2), an alcohol extract of soybean meal. Both SoyMeal and SoyMol caused enteritis-like changes in the distal intestine, which were not observed in fish fed the Contr1, Contr2, SoyConc1, or SoyConc2 diets. There were significant differences (P < 0.05) in mortality between feeding groups following the A.s. salmonicida challenge: these differences were greatest in fish fed SoyMeal (65.6%), least in fish fed SoyConc1 (60.5%), and intermediate in the fish fed the Contr1 diet (62.9%). The SoyMol diet caused significantly (P < 0.0001) increased levels of both lysozyme and IgM in the mid and distal intestinal mucosa. It is concluded that components of soybean meal and soybean molasses cause an inflammatory response in the distal intestine that may lead to increased susceptibility to furunculosis. [source] Effect of fishmeal substitution with various animal and/or plant protein sources in the diet of the abalone Haliotis discus hannai InoAQUACULTURE RESEARCH, Issue 10 2010Sung Hwoan Cho Abstract The effect of dietary substitution of animal and/or plant protein sources for fishmeal on the growth and body composition of juvenile abalone was determined. Nine experimental diets in triplicate were prepared: Con, SM, PM, CM, SPM, MB, SM+CM, SM+SPM and CM+SPM diets. A 350 g kg,1 fishmeal was included in the Con diet, and the whole fishmeal was substituted with a 580 g kg,1 soybean meal (SM), 335 g kg,1 poultry meal (PM), 370 g kg,1 corn gluten meal (CM), 325 g kg,1 silkworm pupae meal dehydrated (SPM), 590 g kg,1 meat and bone meal (MB), the combined 290 g kg,1 soybean meal and 180 g kg,1 corn gluten meal (SM+CM), 290 g kg,1 soybean meal and 160 g kg,1 silkworm pupae meal dehydrated (SM+SPM) and 180 g kg,1 corn gluten meal and 170 g kg,1 silkworm pupae meal dehydrated (CM+SPM) diets respectively. All experimental diets were iso-nitronic and iso-lipidic. The survival of abalone fed with the SM and SM+SPM diets was higher than that of abalone fed with the CM, SPM, MB and CM+SPM diets. Weight gain of abalone fed with the SM+SPM diet was higher than that of abalone fed with all the other experimental diets, except for that of abalone fed with the SM+CM diet. The combined soybean meal and corn gluten meal (SM+CM) or silkworm pupae meal (SM+SPM) could be replaced with the whole fishmeal in the diet for abalone and improved its performance. [source] Utilization of plant proteins in fish diets: effects of global demand and supplies of fishmealAQUACULTURE RESEARCH, Issue 5 2010Ronald W Hardy Abstract Aquafeed ingredients are global commodities used in livestock, poultry and companion animal feeds. Cost and availability are ditated less by demand from the aquafeed sector than by demand from other animal feed sectors and global production of grains and oilseeds. The exceptions are fishmeal and fish oil; use patterns have shifted over the past two decades resulting in nearly exclusive use of these products in aquafeeds. Supplies of fishmeal and oil are finite, making it necessary for the aquafeed sector to seek alternative ingredients from plant sources whose global production is sufficient to supply the needs of aquafeeds for the foreseeable future. Significant progress has been made over the past decade in reducing levels of fishmeal in commercial feeds for farmed fish. Despite these advances, the quantity of fishmeal used by the aquafeed sector has increased as aquaculture production has expanded. Thus, further reduction in percentages of fishmeal in aquafeeds will be necessary. For some species of farmed fish, continued reduction in fishmeal and fish oil levels is likely; complete replacement of fishmeal has been achieved in research studies. However, complete replacement of fishmeal in feeds for marine species is more difficult and will require further research efforts to attain. [source] Effects of dietary prebiotics on the growth, feed efficiency and non-specific immunity of juvenile red drum Sciaenops ocellatus fed soybean-based dietsAQUACULTURE RESEARCH, Issue 3 2010J Alejandro Buentello Abstract Previous work has demonstrated several benefits of dietary prebiotics for fish. Here, we report the effects of added dietary fructooligosaccharide (FOS), mannanoligosaccharide (MOS), transgalactooligosaccharide (TOS) and GroBiotic® -A (GBA) on the weight gain, feed efficiency and non-specific immunity of juvenile red drum fed soybean meal (SBM)-based diets, in brackish water at 25 ± 1 °C. Test diets incorporated FOS, MOS, TOS or GBA at 10 g kg,1 in place of cellulose in a basal diet with 40% crude protein, half from SBM and half from fishmeal. After a 4-week feeding period, experimental fish were challenged by exposure to Amyloodinium ocellatum, after which growth and survival were monitored for 2 more weeks. Feed efficiency, serum lysozyme and intracellular superoxide anion production were significantly (P<0.05) enhanced by prebiotic supplementation. Likewise, survival after A. ocellatum exposure was significantly higher for fish fed GBA, MOS and TOS (87%, 84% and 78%) versus 58% for fish fed either the FOS or the basal diet. Taken together, these results indicate that inclusion of several prebiotics at 10 g kg,1 of the diet is adequate to improve the feed efficiency of red drum as well as to enhance disease protection. [source] Starch source, screw configuration and injection of steam into the barrel affect the physical quality of extruded fish feedAQUACULTURE RESEARCH, Issue 3 2010Mette Sørensen Abstract This experiment investigated the physical qualities (,Holmen' durability; ,tumbling box' durability; and pellet strength, length and diameter) of extruded fishmeal-based feeds produced using different starch sources, screw configurations, with or without steam injected in the barrel. The starch sources tested were native or pre-gelatinized potato starch (PGS), wheat starch (WS), whole wheat (WW) or a combination of WS and WW. Screw configurations were a polygon element, followed by two left-pitched segments, Polygon_2L; a polygon, followed by one left-pitched and one right-pitched element, Polygon_LR; and no polygon section, alternating left- and right-pitched elements, LRLR. Starch source and screw configuration affected the physical quality the most. Pellets produced with LRLR had the highest ,Holmen' durability (76%), hardness (30 N) and the least diameter (5.3 mm). The lowest durability (37%) and hardness (22 N) were obtained with Polygon_LR. Potato starch yielded higher expansion than cereal starch. The highest ,Holmen' durability was seen for PGS (79%), while WS yielded the lowest value (44%). Injection of steam reduced hardness and ,Holmen' durability, but provided a minor overall contribution to physical quality compared with the starch source and screw configuration. ,Holmen' durability appeared to be suitable to unveil variation (37,79%) in physical quality, while ,Tumbling box' durability (98.4,98.8%) did not prove efficient. [source] Effect of fishmeal replacement with Artemia biomass as a protein source in practical diets for the giant freshwater prawn Macrobrachium rosenbergiiAQUACULTURE RESEARCH, Issue 6 2009Nguyen Thi Ngoc Anh Abstract A 30-day feeding experiment was conducted in 160-L plastic tanks to evaluate the potential use of Artemia biomass as a protein source in practical diets for postlarval Macrobrachium rosenbergii (initial mean weight of 12.12,12.29 mg). Nine isoenergetic and isonitrogenous experimental diets (approximately 40% crude protein) were formulated by replacing levels of the fishmeal (FM) protein difference either with dried or frozen Artemia (0, 25, 50, 75 and 100%). The 0%Artemia treatment, in which Peruvian FM was the only main protein source, was considered to be the control diet. The results showed that prawn postlarvae (PLs) fed the FM control diet had a lower survival (46%) compared with all Artemia diets. Significant differences (P<0.05) were, however, only found at 75% and 100%Artemia protein inclusion levels (survival of 68,77%). A gradual increase in growth performance (live weight gain, specific growth rate and total length) of the prawns was achieved on increasing dietary inclusion of Artemia protein. Additionally, the size distribution exhibited the same response as growth performance. However, prawns fed the frozen Artemia diets showed a better performance than the ones fed the dried Artemia diets. It can be suggested that Artemia biomass may totally replace FM in practical diets for PLs of the freshwater prawn M. rosenbergii. [source] | ||||||||||||||||