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Fish Sperm DNA (fish + sperm_dna)
Selected AbstractsDetermination of nucleic acid by [tetra-(3-methoxy-4-hydroxyphenyl)],Tb3+ porphyrin as the fluorescence spectral probe in bis(2-ethylhexyl)sulfosuccinate sodium salt micelle systemLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 4 2009Xin Chen Abstract A new system for the determination of nucleic acid by rare earth metallic porphyrin of [tetra-(3-methoxy-4-hydroxyphenyl)],Tb3+ [T(3-MO-4HP),Tb3+] porphyrin as fluorescence spectral probe has been developed in this paper. Nucleic acid can enhance the fluorescence intensity of the T(3-MO-4HP),Tb3+ porphyrin in the presence of bis(2-ethylhexyl)sulfosuccinate sodium salt(AOT) micelle. In pH 8.00 Tris,HCl buffer solution, under optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of nucleic acids in the range of 0.05,3.00 µg mL,1 for calf thymus DNA (ct DNA) and 0.03,4.80 µg mL,1 for fish sperm DNA(fs DNA). Their detection limits are 0.03 and 0.01 µg mL,1, respectively. In addition, the binding interaction mechanism between T(3-MO-4HP),Tb3+ porphyrin and ct DNA is also investigated by resonance scattering and fluorescence spectra. The maximum binding number is calculated by molar ratio method. The new system can be used for the determination of nucleic acid in pig liver, yielding satisfactory results. Copyright © 2009 John Wiley & Sons, Ltd. [source] Synthesis, characterization and biological studies of alkenyl-substituted titanocene(IV) carboxylate complexesAPPLIED ORGANOMETALLIC CHEMISTRY, Issue 9 2010Goran N. Kalu, erovi Abstract The carboxylate compounds [Ti(,5 -C5H5)(,5 -C5H4{CMe2(CH2CH2CHCH2)})(O2CCH2SXyl)2] (2; Xyl = 3,5-Me2C6H3) and [Ti(,5 -C5H5)(,5 -C5H4{CMe2(CH2CH2CHCH2)})(O2CCH2SMesl)2] (3; Mes 1 = 2,4,6-Me3C6H2) were synthesized by the reaction of [Ti(,5 -C5H5)(,5 -C5H4{CMe2(CH2CH2CHCH2)})Cl2] (1) with 2 equivalents of xylylthioacetic acid or mesitylthioacetic acid, respectively. Compounds 2 and 3 were characterized by spectroscopic methods. The cytotoxic activity of 1,3 was tested against human tumor cell lines from four different histogenic origins,8505C (anaplastic thyroid cancer), DLD-1 (colon cancer) and the cisplatin sensitive A253 (head and neck cancer) and A549 (lung carcinoma),and compared with those of the reference complex [Ti(,5 -C5H5)2Cl2] (R1) and cisplatin. Surprisingly, the cytotoxic activities of the carboxylate derivatives were lower than those of their corresponding dichloride analogue (1). However, complexes 1,3 were more active than titanocene dichloride against all the studied cells with the exception of complex 2 against A253 and A549 cell lines. DNA-interaction tests were also carried out. Solutions of all the studied complexes were treated with different concentrations of fish sperm DNA, observing modifications of the UV spectra with intrinsic binding constants of 2.99 × 105, 2.45 × 105, and 2.35 × 105M,1 for 1,3. Structural studies based on density functional theory calculations of 2 and 3 were also carried out. Copyright © 2010 John Wiley & Sons, Ltd. [source] Structure, DNA Binding Studies and Cytotoxicity of Complex [Pd(phen)(L -asp)]·3H2OCHINESE JOURNAL OF CHEMISTRY, Issue 6 2009Enjun GAO Abstract The palladium(II) complex of [Pd(phen)(L -asp)]·3H2O (phen=1,10-phenanthroline, H2L-asp=L -aspartic acid) has been synthesized from a solution reaction and analyzed by elemental analyses, 1H NMR and IR spectra. Moreover, the complex has been structurally characterized by single-crystal X-ray diffractometry. The cytotoxicity assay of the complex and cis -DDP as reference substance against three different cancer cell lines (Hela, Hep-G2 and KB) has been conducted. The results show that the Pd complex exhibits higher cytotoxicity against Hela system. The study on the interaction of the Pd complex with fish sperm DNA (FS-DNA) has been performed with diverse spectroscopic techniques, showing that the complex is bound to the fish sperm DNA via an intercalative mode. Gel electrophoresis assay demonstrates the ability of the complex to cleave the pBR 322 plasmid DNA. [source] Resonance Light Scattering Imaging Detection of Single Suprahelical Species of DNA Induced by Porphine-5,10,15,20-tetrakis(p -phenyltrimethylaminium)CHINESE JOURNAL OF CHEMISTRY, Issue 1 2006Xi-Dong Liu Abstract A resonance light scattering (RLS) imaging method was proposed based on imaging and measuring the RLS features of single suprahelical species of DNA, and its application to DNA assay was also investigated. In acidic medium, porphine-5,10,15,20-tetrakis(p -phenyltrimethylaminium) (PTPTMA), could stack along the molecular surface of DNA with the mode of long-range assembly to induce the formation of suprahelical species of DNA, resulting in strong RLS signals in the range of 450,510 nm. Under the excitation of 488 nm light beam of argon ion laser source, single suprahelical species could be observed with the aid of a common microscope due to the strong scattered light emitted by the suprahelical species. By capturing the RLS images of the single suprahelical species with a cooled charge coupled device (CCD) camera, and analyzing the RLS data, herein an RLS imaging method of DNA was proposed based on the linear relationship between the counts of suprahelical species in the detection focus plane and the concentration of DNA in nanograms. When 1.8??µmol/L PTPTMA was employed, both calf thymus DNA (ct DNA) and fish sperm DNA (fs DNA) in the range of 25,1100 ng/mL could be detected with the limits of detection lower than 25 ng/mL (3,). Four synthetic samples were detected satisfactorily with relative standard deviations less than 5.1%. [source] | ||||||||||