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Fish Mortality (fish + mortality)
Selected Abstracts163 Identification of Euglenoids That Produce Ichthyotoxin(S) (Euglenophyta)JOURNAL OF PHYCOLOGY, Issue 2003R. E. Triemer Diatoms, dinoflagellates, pelagiophytes, prymnesiophytes, and cyanobacteria are the only divisions of microalgae known to produce toxins. We now report toxin production by freshwater members of the genus Euglena. Fish mortalities (sheepshead minnows, catfish, striped bass, and tilapia) have been observed following exposure in the field to Euglena blooms and in the laboratory when exposed to unialgal isolates of two species of Euglena (E. sanguinea Ehrenberg and E. granulata (Klebs) Lemm.). Three toxic fractions have been isolated from unialgal isolates of both species, and include both water soluble and lipophilic compounds having ichthyotoxic activity. The toxins are stable at ,80°C for at least 60 days and are heat stable to 30°C. Erratic swimming behavior of fish suggests a neurological toxin. This is the first report of fish kills by any freshwater algal taxa from both field and laboratory studies. [source] RAPHIDOPHYCEAE [CHADEFAUD EX SILVA] SYSTEMATICS AND RAPID IDENTIFICATION: SEQUENCE ANALYSES AND REAL-TIME PCR ASSAYS,JOURNAL OF PHYCOLOGY, Issue 6 2006Holly A. Bowers Species within the class Raphidophyceae were associated with fish kill events in Japanese, European, Canadian, and U.S. coastal waters. Fish mortality was attributable to gill damage with exposure to reactive oxygen species (peroxide, superoxide, and hydroxide radicals), neurotoxins, physical clogging, and hemolytic substances. Morphological identification of these organisms in environmental water samples is difficult, particularly when fixatives are used. Because of this difficulty and the continued global emergence of these species in coastal estuarine waters, we initiated the development and validation of a suite of real-time polymerase chain reaction (PCR) assays. Sequencing was used to generate complete data sets for nuclear encoded small-subunit ribosomal RNA (SSU rRNA; 18S); internal transcribed spacers 1 and 2, 5.8S; and plastid encoded SSU rRNA (16S) for confirmed raphidophyte cultures from various geographic locations. Sequences for several Chattonella species (C. antiqua, C. marina, C. ovata, C. subsalsa, and C. verruculosa), Heterosigma akashiwo, and Fibrocapsa japonica were generated and used to design rapid and specific PCR assays for several species including C. verruculosa Hara et Chihara, C. subsalsa Biecheler, the complex comprised of C. marina Hara et Chihara, C. antiqua Ono and C. ovata, H. akashiwo Ono, and F. japonica Toriumi et Takano using appropriate loci. With this comprehensive data set, we were also able to perform phylogenetic analyses to determine the relationship between these species. [source] Supplementing the feed of pikeperch [Sander lucioperca (L.)] juveniles with MacroGard and its influence on nonspecific cellular and humoral defense mechanismsAQUACULTURE RESEARCH, Issue 4 2009Andrzej K Siwicki Abstract This study examined the influence of ,-1.3/1.6-glucan (MacroGard) on the innate immune system in healthy pikeperch (Sander lucioperca) juveniles. MacroGard was fed in a pelleted ration of 1 or 2 g kg,1 feed for 6 weeks. The control group of fish was fed feed with no supplement. Blood, pronephros and spleen samples for the study of nonspecific cellular and humoral defense mechanisms were collected from 10 fish from each group. No changes were observed in either the behaviour or the health of the fish throughout the rearing period. Fish mortality was not noted in any of the groups. Additionally, the supplementation of feed with MacroGard did not have a significant impact on the fish growth rate as expressed in both absolute and relative values (P>0.05). The condition of the fish from the experimental group did not differ from that of the control group specimens, and the feed conversion ratios were also very similar (P>0.05). The results showed that MacroGard administered in two doses significantly (P<0.05) activated the metabolic activity and potential killing activity of spleen phagocytes, compared with the control-fed pikeperch. The highest phagocyte activity was observed with a dose of 2 g kg,1. The proliferative response of pronephros lymphocytes indicated a similar pattern. MacroGard significantly increased proliferative response of lymphocytes stimulated by mitogens (P<0.05). MacroGard also significantly increased the lysozyme activity and total immunoglobulin levels in the serum. In this study neither significantly different ceruloplasmine activity or total protein level in serum were observed. The result showed that MacroGard at the optimal dose of 1 g kg,1 feed and a twofold higher dose does not have a negative influence on the hepatocytes or increased nonspecific cell- and humoral-mediated immunity in healthy pikeperch. [source] Predicting and managing the effects of hypersalinity on the fish community in solar salt fields in north-western AustraliaJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2 2006B. W. Molony Summary Five concentrator ponds (CPs) of a solar salt field in Port Hedland, Western Australia were sampled by seine and gill nets over a 12-month period in order to describe the fish community and examine relationships between diversity, abundance and catch per unit effort (CPUE) with salinity. Salinity varied between 40.2 and 113.7, during the sampling period. Forty-one species of fishes were recorded from the CPs, with fewer species recorded from CPs of higher salinity. A significant inverse relationship was identified between salinity and the number of species (diversity) captured in gill nets, indicating that one species is lost with every 16, increase in salinity. A significant relationship between salinity and CPUE was also identified with gill-net samples, indicating a reduction of 1 kg h,1 with every increase in salinity of 5.5,. As CPs are connected by one-way flaps, fish movements are only possible into CPs of higher salinity. Thus, reductions in diversity, abundance and CPUE suggested fish mortalities, likely as a result of maximum or rapidly changing salinities exceeding the tolerance ability of individual species. As fish kills are not infrequent events in solar salt fields and result in economic losses due to loss of production and clean-up costs, the results may allow managers to identify high risk species and times of year of fish kills by using salinity measurements. Commercial, indigenous and/or recreational fishing opportunities are viable options for reducing fish biomasses within the CPs and are discussed. Although absolute salinity values were higher than those recorded from tropical Australian estuaries, salinity deviations within each CP are similar to other estuaries and the effect on the ichthyo-community is likely to be similar. [source] Lancefield group C Streptococcus dysgalactiae infection responsible for fish mortalities in JapanJOURNAL OF FISH DISEASES, Issue 12 2004R Nomoto Abstract A Lancefield serological group C Streptococcus sp. was isolated from cultured amberjack, Seriola dumerili Risso, and yellowtail, Seriola quinqueradiata Temminck and Schlegel, immunized with Lactococcus garvieae commercial vaccines in Japan. The isolated bacteria were Gram-positive cocci, auto-aggregating in saline, morphologically long chains in growth medium, catalase negative and , -haemolytic on blood agar. An almost complete gene sequence of the 16S rDNA of two isolates was determined and compared with that of bacterial strains in the database. The isolates were identified as Streptococcus dysgalactiae based on the results of the 16S rDNA sequence, the bacteriological properties and the Lancefield serological grouping. Oligonucleotide primers specifically designed for the 16S,23S rDNA intergenic spacer region of S. dysgalactiae amplified a gene from all the fish isolates, as well as the type strains , -haemolytic S. dysgalactiae subsp. dysgalactiae ATCC430738 and , -haemolytic S. dysgalactiae subsp. equisimilis ATCC35666, but not those of S. equi ATCC33398, Lactococcus garvieae ATCC43921 and L. garvieae KG9408. The severe necrotic lesions of the caudal peduncle seen in experimentally infected fish were similar to those seen in naturally infected fish. [source] Verrucophora farcimen gen. et sp. nov. (Dictyochophyceae, Heterokonta),a bloom-forming ichthyotoxic flagellate from the Skagerrak, Norway,JOURNAL OF PHYCOLOGY, Issue 5 2007Bente Edvardsen Since 1998, a heterokont flagellate initially named Chattonella aff. verruculosa has formed recurrent extensive blooms in the North Sea and the Skagerrak, causing fish mortalities. Cells were isolated from the 2001 bloom off the south coast of Norway, and monoalgal cultures were established and compared with the Chattonella verruculosa Y. Hara et Chihara reference strain NIES 670 from Japan. The cells in Norwegian cultured isolates were very variable in size and form, being large oblong (up to 34 ,m long) to small rounded (5,9 ,m in diameter) with two unequal flagella, numerous chloroplasts, and mucocysts. The SSU and partial LSU rDNA sequences of strains from Norway and Japan were compared and differed by 0.4% (SSU) and 1.3% (LSU), respectively. Five strains from Norway were identical in the LSU rDNA region. Phylogenetic analyses based on heterokont SSU and concatenated SSU + LSU rDNA sequences placed C. aff. verruculosa and the Japanese C. verruculosa within the clade of Dictyochophyceae, with the picoflagellate Florenciella parvula Eikrem as the closest relative. Ultrastructure, morphology, and pigment composition supported this affinity. We propose the name Verrucophora farcimen sp. et gen. nov. for this flagellate and systematically place it within the class Dictyochophyceae. Our studies also show that C. verruculosa from Japan is genetically and morphologically different but closely related to V. farcimen. The species is transferred from the class Raphidophyceae to the class Dictyochophyceae and renamed Verrucophora verruculosa. We propose a new order, Florenciellales, to accommodate V. farcimen, V. verruculosa, and F. parvula. [source] A NEW LARVAL FISH BIOASSAY FOR TESTING THE PATHOGENICITY OF PFIESTERIA SPP. (DINOPHYCEAE),JOURNAL OF PHYCOLOGY, Issue 3 2003Vincent J. Lovko Water quality, microbial contamination, prior fish health, and variable results have been major impediments to identifying the cause and mechanism of fish mortality in standard aquarium-format Pfiesteria bioassays. Therefore, we developed a sensitive 96-h larval fish bioassay for assessing Pfiesteria spp. pathogenicity using six-well tissue culture plates and 7-day-old larval cyprinodontid fish. We used the assay to test pathogenicity of several clonal lines of Pfiesteria piscicida Steidinger and Burkholder and P. shumwayae Glasgow and Burkholder that had been cultured with algal prey for 2 to 36 months. The P. shumwayae cultures exhibited 80%,100% cumulative mortality in less than 96 h at initial zoospore densities of approximately 1000 cells·mL,1. No fish mortalities occurred with P. piscicida at identical densities or in controls. In a dose-response assay, we demonstrated a strong positive correlation between dinospore density and fish mortality in a highly pathogenic culture of P. shumwayae, generating a 96-h LD50 of 108 zoospores·mL,1. Additionally, we applied the assay to evaluate a 38-L P. shumwayae bioassay that was actively killing fish and compared results with those from exposures of juvenile tilapia (Oreochromis niloticus) in a 500-mL assay system. Water from the fish-killing 38-L assay was filtered and centrifuged to produce fractions dominated by dinoflagellates, bacteria, or presumed ichthyotoxin (cell-free fraction). After 96 h, the larval fish assay exhibited 50%,100% cumulative mortality only in fractions containing dinoflagellates, with no mortalities occurring in the other fractions. The 500-mL bioassay with tilapia produced inconsistent results and demonstrated no clear correlation between mortality and treatment. The new larval fish bioassay was demonstrated as a highly effective method to verify and evaluate dinoflagellate pathogenicity. [source] Testing for trends in the violation frequency of an environmental threshold in riversENVIRONMETRICS, Issue 1 2009Lieven Clement Abstract Nutrient pollution in rivers is a common problem. It can provoke algae blooms which are related to increased fish mortality. To restore the water status, the regulator recently has promulgated more restrictive regulations. In Flanders for instance, the government has introduced several manure decrees (MDs) to restrict nutrient pollution. Environmental regulations are commonly expressed in terms of threshold levels. This provides a binary response to the decision maker. To handle such data, we propose the use of marginalised generalised linear mixed models. They provide valid inference on trends in the exceedance frequency. The spatio-temporal dependence of the river monitoring network is incorporated by the use of a latent variable. The temporal dependence is assumed to be AR(1) and the spatial dependence is derived from the river topology. The mean model contains a term for the trend and corrects for seasonal variation. The model formulation allows an assessment on the level of individual sampling locations and on a more regional scale. The methodology is applied to a case study on the river Yzer (Flanders). It assesses the impact of the MDs on the violation probability of the nitrate standard. A trend change is detected after the introduction of the second MD. Copyright © 2008 John Wiley & Sons, Ltd. [source] Participatory research methods in environmental science: local and scientific knowledge of a limnological phenomenon in the Pantanal wetland of BrazilJOURNAL OF APPLIED ECOLOGY, Issue 4 2000D.F. Calheiros Summary 1.,Participatory research methodologies incorporating local knowledge are important to the success of ecological research and the sustainable management of natural systems. However, methods of this type are not commonly employed in the natural sciences. 2.,We adopted a scientifically rigorous ethnographic research methodology to incorporate local knowledge into understanding a natural limnological phenomenon in the Brazilian Pantanal. Known locally as ,dequada', it is associated with fish kills. 3.,Using primarily open-ended questions and semi-structured interviews, 30 older head-of-household men were interviewed, by the same interviewer, in a small community representative of the few local riverside settlements. Their opinions were then contrasted with current scientific knowledge. 4.,In concordance with the scientific community, the local community cited decomposition of organic material as the principal cause of fish mortality due to the dequada. Local people therefore can have a well-founded understanding of their environment. 5.,This study demonstrates the importance of incorporating local knowledge to corroborate and, often, to guide the process of scientific inquiry. In this case, local knowledge added to scientific knowledge by providing a more complete understanding of the management and conservation of a natural system. We recommend that ecologists should be ready to acknowledge that local understanding can be greater than that of ,outsiders'. [source] A NEW LARVAL FISH BIOASSAY FOR TESTING THE PATHOGENICITY OF PFIESTERIA SPP. (DINOPHYCEAE),JOURNAL OF PHYCOLOGY, Issue 3 2003Vincent J. Lovko Water quality, microbial contamination, prior fish health, and variable results have been major impediments to identifying the cause and mechanism of fish mortality in standard aquarium-format Pfiesteria bioassays. Therefore, we developed a sensitive 96-h larval fish bioassay for assessing Pfiesteria spp. pathogenicity using six-well tissue culture plates and 7-day-old larval cyprinodontid fish. We used the assay to test pathogenicity of several clonal lines of Pfiesteria piscicida Steidinger and Burkholder and P. shumwayae Glasgow and Burkholder that had been cultured with algal prey for 2 to 36 months. The P. shumwayae cultures exhibited 80%,100% cumulative mortality in less than 96 h at initial zoospore densities of approximately 1000 cells·mL,1. No fish mortalities occurred with P. piscicida at identical densities or in controls. In a dose-response assay, we demonstrated a strong positive correlation between dinospore density and fish mortality in a highly pathogenic culture of P. shumwayae, generating a 96-h LD50 of 108 zoospores·mL,1. Additionally, we applied the assay to evaluate a 38-L P. shumwayae bioassay that was actively killing fish and compared results with those from exposures of juvenile tilapia (Oreochromis niloticus) in a 500-mL assay system. Water from the fish-killing 38-L assay was filtered and centrifuged to produce fractions dominated by dinoflagellates, bacteria, or presumed ichthyotoxin (cell-free fraction). After 96 h, the larval fish assay exhibited 50%,100% cumulative mortality only in fractions containing dinoflagellates, with no mortalities occurring in the other fractions. The 500-mL bioassay with tilapia produced inconsistent results and demonstrated no clear correlation between mortality and treatment. The new larval fish bioassay was demonstrated as a highly effective method to verify and evaluate dinoflagellate pathogenicity. [source] Differences in the impacts of dams on the dynamics of salmon populationsANIMAL CONSERVATION, Issue 4 2001Phillip S. Levin Modern concrete dams have devastated fish populations world-wide. However, dams vary greatly in how they are engineered and operated, and thus pose a range of threats to riverine fauna. Understanding the differences in the impacts of dams is critical for setting conservation priorities. We used a modified BACI (before-after-control-impact) sampling design as a means to quantify the effects of dams on spring/summer chinook salmon in two watersheds (Snake and Upper Columbia Rivers) of the Columbia River Basin, USA. The construction of four dams in the Columbia River Basin from 1966 to 1975 allowed us to test the hypothesis that the presence of these dams does not affect the abundance, survival and population growth of chinook salmon. In both the Snake and Upper Columbia Rivers, there was a significant decline from the period before dams were constructed (1959-65) to the period after dams were constructed (1980-90). In the Upper Columbia River, declines in productivity or population performance (measured as recruits per spawner or Ricker function residuals) were greater than in the control region. On the other hand, patterns of fish productivity in the Snake River were similar to those seen in the control region. The disparity between fates of Upper Columbia and Snake River populations points to the differences between regions in current efforts to reduce fish mortality associated with dams. Our analysis suggests that dams in the Upper Columbia River, but not Snake River, are a potential force preventing recovery of endangered salmon populations. [source] Live Spirulina (Arthrospira platensis) as a growth and immunity promoter for Nile tilapia, Oreochromis niloticus (L.), challenged with pathogenic Aeromonas hydrophilaAQUACULTURE RESEARCH, Issue 9 2009Mohsen Abdel-Tawwab Abstract The use of antibiotics to control fish disease is one of the constraints in tilapia farming. Hence, the use of probiotic and live organisms as alternative strategies has received much more attention. This study was undertaken to evaluate the use of Spirulina (Arthrospir platensis) as a growth and immunity promoter for Nile tilapia, Oreochromis niloticus (L.). Fish (1.88 g) were randomly distributed at 20 fish per 100 L aquarium and fed a diet containing either 0.0, 1.25, 2.5, 5.0, 7.5 or 10.0 g Spirulina kg,1 diet for 12 weeks. After the feeding trial, fish of each treatment were challenged by pathogenic Aeromonas hydrophila, which was given by an interperitoneal injection, and they were kept under observation for 10 days to record any abnormal clinical signs and the daily mortality rate. The growth-promoting influence of Spirulina was observed with fish, and the optimum growth and feed utilization were obtained at 5.0 g Spirulina kg,1 diet. No significant changes were observed in fish survival among the different treatments. Spirulina supplementation improved the protein and lipid contents in fish bodies without significant differences among them, especially when fed 2.5,10.0 g kg,1 diet, but no significant differences in the moisture and ash contents were observed among different treatments. The physiological parameters were improved when fish were fed a Spirulina-enriched diet. However, the highest red blood cells, white blood cells and nitro blue tetrazolium values were obtained at 5.0,10.0 g Spirulina kg,1 diet; meanwhile, the lowest value was obtained in the control. Total fish mortality due to A. hydrophila infection decreased with an increase in the Spirulina level in fish diets. These results indicate that Spirulina supplement is promising as an alternative method to antibiotics for disease prevention in tilapia aquaculture, and the optimum level of Spirulina in the fish diet should be 5.0,10.0 g kg,1 diet. [source] Eugenol as an efficacious anaesthetic for tambaqui, Colossoma macropomum (Cuvier)AQUACULTURE RESEARCH, Issue 11 2005Rodrigo Roubach Abstract Anaesthetics are important in fish culture to reduce handling stress and mortality. Eugenol is a promising anaesthetic because of its low cost, efficacy, safety margin for fish and lack of toxicity to humans. The goal of this study was to establish a protocol using eugenol as a fish anaesthetic for tambaqui Colossoma macropomum (Cuvier), and provide information for regulating authorities on establishing safety dosage protocols for its use. Juvenile and sub-adult tambaqui were first individually exposed to doses of 35, 50, 65, 85, 100 or 135 mg L,1 eugenol for 10 min. A second experiment examined the effect of the duration of exposure to eugenol on the time required for recovery and survival of tambaqui. A eugenol dose of 65 mg L,1 was adequate to induce fish of both sizes into a surgical anaesthetic state, and recovery time was similar for dosages up to 100 mg L,1. Exposure to the ideal dose (65 mg L,1) for up to 30 min did not cause fish mortality. Fish blood glucose values were similar for all the tested eugenol doses as well as with the benzocaine control. The results show that eugenol is an efficient and safe anaesthetic for tambaqui. [source] | |||||||||||||