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First Approach (first + approach)
Selected AbstractsA First Approach to the cis-trans-cis -Photocyclodimers of 1,2-NaphthoquinoneHELVETICA CHIMICA ACTA, Issue 9 2007Kerstin Schmidt Abstract On irradiation (,=350,nm), 1,2-naphthoquinone (=naphthalene-1,2-dione) monoacetals 1 are converted quantitatively to mixtures of the cis-trans-cis -photocyclodimers 2 and 3. Careful hydrolysis of each of the (parent) pentacyclic diacetals 2a and 3a affords the , rather unstable , compounds 4 and 5, respectively. [source] Modification of carbon nanotubes and its effect on properties of carbon nanotube/epoxy nanocompositesPOLYMER COMPOSITES, Issue 10 2009Zdenko, pitalský We have studied an effect of three types of modifications of carbon nanotubes (CNTs) on dispersion and mechanical properties of final epoxy-amine based nanocomposites. First approach includes end-walled covalent chemical modification at the ends of nanotubes. The second one is side-walled covalent chemical modification along the whole length of nanotubes. The third procedure is noncovalent, physical modification done by the CNT surface coating with polyaniline. The modification of nanotubes was determined by X-ray photoelectron spectroscopy. The prepared epoxy-amine nanocomposites were characterized by dynamic-mechanical analysis, tensile testing, light microscopy, transmission electron microscopy, and thermogravimetry. We observed an improvement of the mechanical properties and the thermal stability by addition of the carbon nanotubes to the epoxy matrix. The strong interactions between the nanotube and the polymer matrix were discovered in the nanocomposites with physically modified nanotubes. POLYM. COMPOS., 2009. © 2008 Society of Plastics Engineers [source] First approach of a methodological set-up for selenomethionine chiral speciation in breast and formula milk using high-performance liquid chromatography coupled to atomic fluorescence spectroscopyAPPLIED ORGANOMETALLIC CHEMISTRY, Issue 6 2007J. L. Gómez-Ariza Abstract The chiral speciation of selenomethionine in breast and formula milk based on species separation by high-performance liquid chromatography followed by online microwave-assisted digestion and detection with hydride generation atomic fluorescence spectrometry (HPLC-MAD-HG-AFS) requires severe sample manipulation to avoid matrix influence. Sample clean-up for fat and protein elimination using centrifugation and ultrafiltration was optimized, and selenomethionine preconcentration based on cation exchange solid-phase extraction was studied and optimized. The resulting procedure is suitable for chiral selenium speciation in infant milk with detection limits of 3.1 and 3.5 ng ml,1 as Se for L -selenomethionine and D -selenomethionine, respectively. The time necessary for the analysis, about 90 min, including sample clean-up, analyte preconcentration and chromatographic separation, makes the approach suitable for routine analysis. Copyright © 2007 John Wiley & Sons, Ltd. [source] Teaching differential equations in different environments: A first approachCOMPUTER APPLICATIONS IN ENGINEERING EDUCATION, Issue 3 2010Filipa Carneiro Abstract The use of specific softwares was the basis for a new approach for teaching ordinary and partial differential equations, in the field of heat transfer and fluid mechanics. The main objective was to enhance learning effectiveness of Numerical Methods in the post-graduate course of Polymers Engineering at the University of Minho. This degree takes place into two different environments: at the university campus and at the industrial field. Different commercial codes were used, namely EXCEL, MATLAB, and FLUENT, as well as two tools developed in house at University of Minho: CoNum and a graphics application PDE v.1. Lectures were based on videoconferencing and other web utilities. The teaching methodology presented and discussed in this article was well received and accepted by the post-graduate students, motivating teachers to improve their teaching/learning strategies. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 18: 555,562, 2010; View this article online at wileyonlinelibrary.com; DOI 10.1002/cae.20231 [source] A Hybrid Approach to Multiple Fluid Simulation using Volume FractionsCOMPUTER GRAPHICS FORUM, Issue 2 2010Nahyup Kang Abstract This paper presents a hybrid approach to multiple fluid simulation that can handle miscible and immiscible fluids, simultaneously. We combine distance functions and volume fractions to capture not only the discontinuous interface between immiscible fluids but also the smooth transition between miscible fluids. Our approach consists of four steps: velocity field computation, volume fraction advection, miscible fluid diffusion, and visualization. By providing a combining scheme between volume fractions and level set functions, we are able to take advantages of both representation schemes of fluids. From the system point of view, our work is the first approach to Eulerian grid-based multiple fluid simulation including both miscible and immiscible fluids. From the technical point of view, our approach addresses the issues arising from variable density and viscosity together with material diffusion. We show that the effectiveness of our approach to handle multiple miscible and immiscible fluids through experiments. [source] IR Laser-Induced Carbothermal Reduction of SilicaEUROPEAN JOURNAL OF INORGANIC CHEMISTRY, Issue 26 2008Markéta Urbanová Abstract Pulsed IR-laser irradiation of silica in the presence of gaseous hydrocarbons (benzene or ethyne) results in carbothermal reduction of silica by hydrocarbon decomposition products and allows deposition of amorphous solids which were analyzed by FTIR, Raman, X-ray photoelectron and Auger spectra and by electron microscopy and revealed as nanosized carbon,silicon oxycarbide composites containing crystalline silica domains. The reported IR laser-induced process is the first approach to deposition of nanosized carbon,silicon oxycarbide composites. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008) [source] Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L.FEBS JOURNAL, Issue 10 2007Catarina Pimentel Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese. We have been characterizing cardosins at the biochemical, structural and molecular levels. In this study, we show that the cardoon aspartic proteases are encoded by a multigene family. The genes for cardosin A and cardosin B, as well as those for two new cardoon aspartic proteases, designated cardosin C and cardosin D, were characterized, and their expression in C. cardunculus L. was analyzed by RT-PCR. Together with cardosins, a partial clone of the cyprosin B gene was isolated, revealing that cardosin and cyprosin genes coexist in the genome of the same plant. As a first approach to understanding what dictates the flower-specific pattern of cardosin genes, the respective gene 5, regulatory sequences were fused with the reporter ,-glucuronidase and introduced into Arabidopsis thaliana. A subsequent deletion analysis of the promoter region of the cardosin A gene allowed the identification of a region of approximately 500 bp essential for gene expression in transgenic flowers. Additionally, the relevance of the leader intron of the cardosin A and B genes for gene expression was evaluated. Our data showed that the leader intron is essential for cardosin B gene expression in A. thaliana. In silico analysis revealed the presence of potential regulatory motifs that lay within the aforementioned regions and therefore might be important in the regulation of cardosin expression. [source] Quantification of Grafting Densities Achieved via Modular "Grafting-to" Approaches onto Divinylbenzene MicrospheresADVANCED FUNCTIONAL MATERIALS, Issue 12 2010Leena Nebhani Abstract The surface modification of divinylbenzene (DVB)-based microspheres is performed via a combination of reversible addition fragmentation chain transfer (RAFT) polymerization and rapid hetero-Diels,Alder (HDA) chemistry with the aim of quantifying the grafting densities achieved using this "grafting-to" method. Two variants of the RAFT-HDA concept are employed to achieve the functionalization of the microspheres. In the first approach, the microspheres are functionalized with a highly reactive diene, i.e., cyclopentadiene, and are subsequently reacted with polystyrene chains (number-averaged molecular weight, Mn,=,4200,g,mol,1; polydispersity index, PDI,=,1.12.) that carry a thiocarbonyl moiety functioning as a dienophile. The functionalization of the microspheres is achieved rapidly under ambient conditions, without the aid of an external catalyst. The surface grafting densities obtained are close to 1.2,×,1020 chains per gram of microspheres. In the second approach, the functionalization proceeds via the double bonds inherently available on the microspheres, which are reacted with poly(isobornyl acrylate) chains carrying a highly dienophilic thiocarbonyl functionality; two molecular weights (Mn,=,6000,g,mol,1, PDI,=,1.25; Mn,=,26,000,g,mol,1, PDI,=,1.26) are used. Due to the less reactive nature of the dienes in the second approach, functionalization is carried out at elevated temperatures (T,=,60,°C) yet in the absence of a catalyst. In this case the surface grafting density is close to 7,chains,nm,2 for Mn,=,6000,g,mol,1 and 4,chains,nm,2 for Mn,=,26,000,g,mol,1, or 2.82,×,1019 and 1.38,×,1019,chains g,1, respectively. The characterization of the microspheres at various functionalization stages is performed via elemental analysis for the quantification of the grafting densities and attenuated total reflectance (ATR) IR spectroscopy as well as confocal microscopy for the analysis of the surface chemistry. [source] Why are species' body size distributions usually skewed to the right?FUNCTIONAL ECOLOGY, Issue 4 2002Jan Koz, owski Summary 1.,Species' body size distributions are right-skewed, symmetric or left-skewed, but right-skewness strongly prevails. 2.,Skewness changes with taxonomic level, with a tendency to high right-skewness in classes and diverse skewness in orders within a class. Where the number of lower taxa allows for analysis, skewness coefficients have normal distributions, with the majority of taxa being right-skewed. 3.,Skewness changes with geographical scale. For a broad range, distributions in a class are usually right-skewed. For a narrower scale, distributions remain right-skewed or become symmetric or even close to uniform. 4.,The prevailing right-skewness of species' body size distributions is explained with macroevolutionary models, the fractal character of the environment, or body size optimization. 5.,Macroevolutionary models assume either size-biased speciation and extinction, or the existence of a constraint on small size. Macroevolutionary mechanisms seem insufficient to explain the pattern of species' body size distributions, but they may operate together with other mechanisms. 6.,Optimization models assume that directional and then stabilizing selection works after speciation events. There are two kinds of optimization approaches to study species' body size distributions. Under the first approach, it is assumed that a single energetic optimum exists for an entire taxon, and that species are distributed around this optimum. Under the second approach, each species has a separate optimum, and the species' body size distribution reflects the distribution of optimal values. 7.,Because not only energetic properties but also mortality are important in determining optimal sizes, only the second approach, that is, seeking the distribution of optimal values, seems appropriate in the context of life-history evolution. This approach predicts diverse shapes of body size distributions, with right-skewness prevailing. [source] Joint full-waveform analysis of off-ground zero-offset ground penetrating radar and electromagnetic induction synthetic data for estimating soil electrical propertiesGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2010D. Moghadas SUMMARY A joint analysis of full-waveform information content in ground penetrating radar (GPR) and electromagnetic induction (EMI) synthetic data was investigated to reconstruct the electrical properties of multilayered media. The GPR and EMI systems operate in zero-offset, off-ground mode and are designed using vector network analyser technology. The inverse problem is formulated in the least-squares sense. We compared four approaches for GPR and EMI data fusion. The two first techniques consisted of defining a single objective function, applying different weighting methods. As a first approach, we weighted the EMI and GPR data using the inverse of the data variance. The ideal point method was also employed as a second weighting scenario. The third approach is the naive Bayesian method and the fourth technique corresponds to GPR,EMI and EMI,GPR sequential inversions. Synthetic GPR and EMI data were generated for the particular case of a two-layered medium. Analysis of the objective function response surfaces from the two first approaches demonstrated the benefit of combining the two sources of information. However, due to the variations of the GPR and EMI model sensitivities with respect to the medium electrical properties, the formulation of an optimal objective function based on the weighting methods is not straightforward. While the Bayesian method relies on assumptions with respect to the statistical distribution of the parameters, it may constitute a relevant alternative for GPR and EMI data fusion. Sequential inversions of different configurations for a two layered medium show that in the case of high conductivity or permittivity for the first layer, the inversion scheme can not fully retrieve the soil hydrogeophysical parameters. But in the case of low permittivity and conductivity for the first layer, GPR,EMI inversion provides proper estimation of values compared to the EMI,GPR inversion. [source] Highly accurate solutions of the bifurcation structure of mixed-convection heat transfer using spectral methodINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 5 2002M. Selmi Abstract This paper is concerned with producing highly accurate solution and bifurcation structure using the pseudo-spectral method for the two-dimensional pressure-driven flow through a horizontal duct of a square cross-section that is heated by a uniform flux in the axial direction with a uniform temperature on the periphery. Two approaches are presented. In one approach, the streamwise vorticity, streamwise momentum and energy equations are solved for the stream function, axial velocity, and temperature. In the second approach, the streamwise vorticity and a combination of the energy and momentum equations are solved for stream function and temperature only. While the second approach solves less number of equations than the first approach, a grid sensitivity analysis has shown no distinct advantage of one method over the other. The overall solution structure composed of two symmetric and four asymmetric branches in the range of Grashof number (Gr) of 0,2 × 106 for a Prandtl number (Pr) of 0.73 has been computed using the first approach. The computed structure is comparable to that found by Nandakumar and Weinitschke (1991) using a finite difference scheme for Grashof numbers in the range of 0,1×106. The stability properties of some solution branches; however, are different. In particular, the two-cell structure of the isolated symmetric branch that has been found to be unstable by the study of Nandakumar and Weinitschke is found to be stable by the current study. Copyright © 2002 John Wiley & Sons, Ltd. [source] An update of EU legislation (Directives and Regulations) on food-related issues (Safety, Hygiene, Packaging, Technology, GMOs, Additives, Radiation, Labelling): presentation and commentsINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 10 2005Ioannis S. Arvanitoyannis Summary This review aims at providing an update of the current European Union (EU) Regulations and Directives on food-related issues. Initially, a brief presentation of EU legislation in terms of structure (horizontal, vertical) was attempted. EU Regulations and Directives were classified into the following categories: food safety (Hazard Analysis Critical Control Points, pesticides, radioactive, hormones, contaminants, freezing , ionisation, food additives, flavourings, packaging), genetically modified organisms, food quality, labelling, food products of plant or animal origin, imports from third countries. Apart from a synoptical presentation of all laws related to the above-mentioned topics, proper tables were compiled where the main points of each law are cited in conjunction with its effect on previous laws (repeal, modification, amendments, replacement). In such a way the reader can rapidly acquire a first approach to the topic of his interest. [source] Aquatic Coleoptera Distribution and Environmental Relationships in a Large Patagonian RiverINTERNATIONAL REVIEW OF HYDROBIOLOGY, Issue 5 2006María Laura Miserendino Abstract The benthic coleopteran assemblages of the Chubut River basin were studied in order to assess the main factors affecting species composition and distribution along the upper, middle and lower catchments. A total of 13 sampling sites were selected and sampled seasonally. Eight taxa and 1,601 individuals were collected during the study. Richness was higher in the main channel of Chubut River at the upper basin than at the middle basin. Beetles were completely absent at the lower basin. Mean monthly density per sites varied from 0 to 85 ind m,2. Stethelmis kaszabi had a more restricted distribution whereas Hemiosus dejeanii, Austrelmis sp. and Austrolimnius spp. were more frequent and abundant. Austrelmis sp. appears as the most tolerant species, especially to higher TSS, ammonia, and conductivity values. Luchoelmis cekalovici was absent in stations associated with urban areas. A Canonical Correspondence Analysis shows that conductivity, total suspended solids, wet width, water temperature and pH were the most important variables structuring beetle assemblages. Land use related variables such as NH4, TP, and NO3 were less important but still significant. An increase in TSS affected negatively the coleopteran community; this could be related to both hydrogeological characteristics and agricultural activities (including overgrazing). This is the first approach to the knowledge of the ecological range of distribution of the coleopteran species in Patagonian rivers. (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Batch and fed-batch carotenoid production by Rhodotorula glutinis,Debaryomyces castellii co-cultures in corn syrupJOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2001P. BuzziniArticle first published online: 21 DEC 200 Aims: Investigations on the production of red pigments by Rhodotorula glutinis on raw substrates of agro-industrial origin may be considered of interest because they represent the first approach to the utilization of these raw materials for biotechnological purposes. Methods and Results:Rhodotorula glutinis DBVPG 3853 was batch and fed-batch co-cultured with Debaryomyces castellii DBVPG 3503 in a medium containing corn syrup as the sole carbon source. Fed-batch co-cultures gave a volumetric production of 8·2 mg total carotenoid l,1, about 150% of that observed in batch co-cultures. The different carotenoid pigments (,-carotene, torulene, torularhodin) were quantified. Conclusions: Oligosaccharides and dextrins of corn syrup could be used profitably for pigment production by R. glutinis DBVPG 3853,D. castellii DBVPG 3503 in co-culture. Significance and Impact of the Study: The above results suggest that the red yeasts belonging to the genus Rhodotorula may have industrial relevance as carotenoid producers. [source] The Measuring Rod of Time: The Example of Swedish Day-finesJOURNAL OF APPLIED PHILOSOPHY, Issue 2 2007LINA ERIKSSON abstract,Time is money', Benjamin Franklin's ,Poor Richard' tells us. But instead of converting time expenditures into monetary equivalents, it makes more sense in many cases to convert money into temporal equivalents. The difficulty in putting a monetary value on time in unpaid household labour, when adjusting the National Accounts, points to the problems of the first approach. The advantages of the latter approach are illustrated by the Swedish system of specifying criminal fines in terms of the number of days the offender would have to work to pay them off. [source] A new multiplex assay allowing simultaneous detection of the inhibition of cell proliferation and induction of cell deathJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 1 2005Józefa W, sierska-G Abstract The efficacy of distinct anti-cancer drugs used in the chemotherapy of human malignancies varies between tumor tissues and depends largely on the ability of the therapeutic agents to simultaneously inhibit cell proliferation and to eliminate malignant cells by apoptosis. Especially, detection of early apoptotic changes seems to be important because early stages of apoptosis differ from those of necrosis. Therefore, the development of a novel test allowing fast and concomitant screening of the anti-proliferative and pro-apoptotic action of a number of anti-cancer drugs is of great interest. For this purpose, we choose as an experimental model a well characterized anti-proliferative and pro-apoptotic effect of cisplatin (CP) on human cervical carcinoma HeLaS3 cells. As previously reported, exposure of HeLaS3 to CP resulted in a concomitant inhibition of cell proliferation and induction of apoptosis in a dose- and time-dependent manner. In the present study we performed two independent approaches. In the first approach, we examined the cell proliferation and activity of caspases-3/7 in two separate microtiter plates using the CellTiter-GloÔ Luminescent Cell Viability Assay and the Caspase-GloÔ 3/7 Assay, respectively. In the second approach, we determined the same parameters sequentially in one microtiter plate by a mutiplexing assay using CellTiter-BlueÔ Cell Viability Assay and Caspase-GloÔ 3/7 Assay. The both approaches gave very similar results indicating that this new multiplexing assay offers an important advantage for simultaneous detection of cell number and activation of caspases-3/7. The new multiplexing assay offers a range of benefits over standard assays. © 2005 Wiley-Liss, Inc. [source] High-resolution H/D exchange studies on the HET-s218,295 prion proteinJOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 5 2005Alexis Nazabal Abstract In a search for improved resolution of hydrogen/deuterium (H/D) exchange experiments analyzed by mass spectrometry (HXMS), we evaluated two methodologies for a detailed structural study of solvent accessibility in the case of the HET-s218,295 prion protein. For the first approach, after incubation in the deuterated solvent, aggregated HET-s218,295 was digested with pepsin and the generated peptides were analyzed by nanospray mass spectrometry in an ion trap, with and without collision-induced dissociation (CID). We compared deuterium incorporation in peptides as determined on peptide pseudomolecular ions and on b and y fragments produced by longer peptides under CID conditions. For both b and y fragment ions, an extensive H/D scrambling phenomenon was observed, in contrast with previous studies comparing CID-MS experiments and 1H NMR data. Thus, the spatial resolution of HXMS experiments could not be improved by means of MS/MS data generated by an ion trap mass spectrometer. In a second approach, the incorporation of deuterium was analyzed by MS for 76 peptides of the HET-s218,289 peptide mass fingerprint, and the use of shared boundaries among peptic peptides allowed us to determine deuteration levels of small regions ranging from one to four amino acids. This methodology led to evidence of highly protected regions along the HET-s218,295 sequence. Copyright © 2005 John Wiley & Sons, Ltd. [source] Towards statistical multicriteria decision modelling: a first approachJOURNAL OF MULTI CRITERIA DECISION ANALYSIS, Issue 6 2002Yves De Smet Abstract Many real life situations result from decisions taken by a very large number of decision makers. Among them, we may cite road traffic congestion, crowding during shopping, equity market behaviour, distribution of holiday destinations, etc. Furthermore, these decisions often depend on the optimisation of several conflicting criteria. In this paper, we introduce a new multicriteria tool based on Markov chains to model and manage these macroscopic phenomena. Finally, the road traffic congestion problem will be considered to illustrate the applicability of our approach. Copyright © 2003 John Wiley & Sons, Ltd. [source] Synthesis of polymeric 1-iminopyridinium ylides as photoreactive polymersJOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 4 2010Daniel Klinger Abstract Two synthetic routes to polymeric 1-imino pyridinium ylides as new photoreactive polymeric architectures were investigated. In the first approach, polymerization of newly synthesized 1-imino pyridinium ylide containing monomers yielding their polymeric analogues was achieved by free radical polymerization. Alternatively, reactive precursor polymers were synthesized and converted into the respective 1-imino pyridinium ylide polymers by polymer analogous reactions on reactive precursor polymers. Quantitative conversion of the reactive groups was achieved with pentafluorophenyl ester containing polymers and newly synthesized photoreactive amines as well as by the reaction of poly(4-vinylbenzoyl azide) with a photoreactive alcohol. The polymers obtained by both routes were examined regarding their photoreaction products and kinetics in solution as well as in thin polymer films. Contact angle measurements of water on the polymer films before and after irradiation showed dramatic changes in the hydrophilicity of the polymers. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 832,844, 2010 [source] Standard errors for EM estimationJOURNAL OF THE ROYAL STATISTICAL SOCIETY: SERIES B (STATISTICAL METHODOLOGY), Issue 2 2000M. Jamshidian The EM algorithm is a popular method for computing maximum likelihood estimates. One of its drawbacks is that it does not produce standard errors as a by-product. We consider obtaining standard errors by numerical differentiation. Two approaches are considered. The first differentiates the Fisher score vector to yield the Hessian of the log-likelihood. The second differentiates the EM operator and uses an identity that relates its derivative to the Hessian of the log-likelihood. The well-known SEM algorithm uses the second approach. We consider three additional algorithms: one that uses the first approach and two that use the second. We evaluate the complexity and precision of these three and the SEM in algorithm seven examples. The first is a single-parameter example used to give insight. The others are three examples in each of two areas of EM application: Poisson mixture models and the estimation of covariance from incomplete data. The examples show that there are algorithms that are much simpler and more accurate than the SEM algorithm. Hopefully their simplicity will increase the availability of standard error estimates in EM applications. It is shown that, as previously conjectured, a symmetry diagnostic can accurately estimate errors arising from numerical differentiation. Some issues related to the speed of the EM algorithm and algorithms that differentiate the EM operator are identified. [source] Homogeneity of fossil assemblages extracted from mine dumps: an analysis of Plio-Pleistocene fauna from South African cavesLETHAIA, Issue 4 2005FRANK SÉNÉGAS Mine dumps associated with limestone cave deposits are common in dolomitic areas of southern Africa. The dumps often contain blocks of breccia, which are rich in micro-mammalian fossils (especially rodents, shrews and bats). Unfortunately, these fossiliferous breccia blocks are out of geological and stratigraphic context. Nevertheless, they provide a large amount of palaeontological material of great interest. In order to use this kind of material, a first approach is to test for homogeneity of the fossil assemblages extracted from the breccia blocks. Fisher's exact test can be used. Two analyses were undertaken. The first was performed on block samples taken in situ from breccia at the Drimolen hominid site. The results indicated that the samples were homogenous, as expected. The second analysis was carried out on different samples extracted from blocks of breccia collected from a dump at the Gondolin site. The results show that it is possible to group several samples in a single representative assemblage. Some blocks could be grouped together and then used to address taphonomic issues. Once these problems are solved, the data set can be used with greater confidence to address matters concerning palaeoenvironmental reconstructions associated with Plio-Pleistocene hominids. [source] A neural network-based approach to determine FDTD eigenfunctions in quantum devicesMICROWAVE AND OPTICAL TECHNOLOGY LETTERS, Issue 9 2009Antonio Soriano Abstract This article combines a Neural Network (NN) algorithm with the Finite Difference Time Domain (FDTD) technique to estimate the eigenfunctions in quantum devices. A NN based on the Least Mean Squares (LMS) algorithm is combined with the FDTD technique to provide a first approach to the confined states in quantum wires. The proposed technique is in good agreement with analytical results and is more efficient than FDTD combined with the Fourier Transform. This technique is used to calculate a numerical approximation to the eigenfunctions associated to quantum wire potentials. The performance and convergence of the proposed technique are also presented in this article. © 2009 Wiley Periodicals, Inc. Microwave Opt Technol Lett 51: 2017,2022, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.24562 [source] Assay of gliadin by real-time immunopolymerase chain reactionMOLECULAR NUTRITION & FOOD RESEARCH (FORMERLY NAHRUNG/FOOD), Issue 5 2003Nadine Henterich Abstract Patients with coeliac disease (gluten-sensitive enteropathy) are intolerant against gliadins from wheat and the respective proteins from related cereals and have to keep a lifelong gluten-free diet. For control of gliadin in gluten-free food sensitive assay techniques are necessary. We developed an immunopolymerase chain reaction (iPCR) assay for gliadin. In this technique immunological detection of gliadin by a monoclonal antibody R5 conjugated with an oligonucleotide is amplified by PCR. For quantification, iPCR was performed as real-time PCR (real-time iPCR) in one step. By means of real-time iPCR, the sensitivity of gliadin analysis was increased more than 30-fold above the level reached by enzyme immunoassay. Real time-iPCR using R5 directly conjugated with oligonucleotide was clearly more sensitive than real time-iPCR applying sequentially biotinylated R5, streptavidin, and biotinylated oligonucleotide. With directly conjugated R5 gliadin was detected at a concentration as low as 0.16 ng/mL corresponding to 16 ,g gliadin/100 g food or 0.16 ppm (corresponding to 0.25 g of food extracted in 10 mL of solvent and 25-fold dilution of the extract prior to analysis). This is the first report applying the highly sensitive technique of iPCR for gliadin analysis. Furthermore, this is the first approach to perform real-time iPCR in one step without changing the reaction vessels after enzyme immunoassay for subsequent PCR analysis thus minimizing risks of contamination and loss of sensitivity. [source] Limits on the detectability of the CMB B-mode polarization imposed by foregroundsMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2005M. Tucci ABSTRACT We investigate which practical constraints are imposed by foregrounds on the detection of the B-mode polarization generated by gravitational waves, in the case of experiments of the type currently being planned. As the B-mode signal is probably dominated by foregrounds at all frequencies, the detection of the cosmological component depends drastically on our ability to remove foregrounds. We provide an analytical expression with which to estimate the level of the residual polarization for Galactic foregrounds, according to the method employed for their subtraction. We interpret this result in terms of the lower limit of the tensor-to-scalar ratio r that allows us to disentangle the cosmological B-mode polarization from the foreground contribution. Polarized emission from extragalactic radio sources and gravitational lensing is also taken into account. As a first approach, we consider the ideal limit of an instrumental noise-free experiment: for full-sky coverage and a resolution of 1°, we obtain a limit of r, 10,4. This value can be improved by high-resolution experiments and, in principle, there is no clear fundamental limit on the detectability of the polarization of gravitational waves. Our analysis is also applied to planned or hypothetical future polarization experiments, taking into account expected noise levels. [source] Female mating success and risk of pre-reproductive death in a protandrous grasshopperOIKOS, Issue 2 2002Raúl Cueva Del Castillo Numerous studies have assessed the adaptive value of protandry for males in several insect species, considering that male emergence is determined by female availability. However, the possible advantage of the time of emergence for females on their mating success in protandrous insect species has only been explored theoretically. By studying the grasshopper Sphenarium purpurascens we evaluated the hypothesis that late emergence could be adaptive for females. If female maturation occurs when the population density is higher and the sex ratio (males/females) is biased to males, their probability of mating increases. Thus, in this study we estimated (1) the opportunity for mating in females as a function of their sexual maturation time, population density, and sex ratio at the moment they reached sexual maturity. In addition, (2) an analysis incorporating female body size and the total number of female matings was performed. Both analyses support the occurrence of protandry in the studied population. Under the first approach, females with intermediate maturation time had a higher probability of being mated than earlier and late matured females. Thus, it suggests that stabilising selection is acting on female maturation time and this may affect selection on male maturation time. Furthermore, the proportion of mated females increased when the sex ratio was biased to males, and stabilising selection on maturation time was detected also. However, the number of matings of a female depended on her body size. Females with larger body size had more matings than smaller ones at the beginning of the reproductive season. Because selection acts differently on maturation time in males and females of S. purpurascens this result is consistent with a condition for the maintenance of protandry in the population. The present results are discussed in the light of the models for the evolution of protandry. [source] Optimal portion control using variable cutter-blade spacing in can-fillingPACKAGING TECHNOLOGY AND SCIENCE, Issue 4 2001F. Omar Abstract In the present context, optimal portion control refers to the process of preparing can-filling portions so that the deviation of the portion weight from a specified target weight is minimized. An approach has been developed for achieving this where a batch of objects is placed in a linearly overlapped optimal arrangement and then cut into portions using a series of parallel blades. The parameters of optimization are the arrangement order, orientation and degree of overlap of the objects. The approach has been demonstrated to produce impressive improvements in the application of fish canning. For this application, two approaches of optimal cutting are compared in the present paper. In one approach, the blade spacing is kept fixed and constant at a predetermined value. In the second approach, the blade spacing is varied for each portion after the objects are placed according to the optimal arrangement, where the target weight distribution is allowed to vary within a tolerance interval. The results presented in this paper indicate that the second approach produces a significantly higher percentage of acceptable portions than the first approach. What is presented are results from computer simulations, utilizing true data as measured from actual batches of fish. The paper demonstrates the potential benefit of the optimal portion control approach when applied in an industrial fish-canning process. Copyright © 2001 John Wiley & Sons, Ltd. [source] MLPA as a screening method of aneuploidy and unbalanced chromosomal rearrangements in spontaneous miscarriagesPRENATAL DIAGNOSIS, Issue 8 2007Dan Diego-Alvarez Abstract Objective The present study aims to validate multiplex ligation-dependent probe amplification (MLPA) technique with subtelomeric probe mixes as a screening method to detect aneuploidy and unbalanced terminal chromosomal rearrangements in spontaneous abortions (SAs). Methods MLPA with P036B and P070 probe mixes was performed on 221 miscarriage DNA samples between the 5th and 24th week of gestation. Cytogenetic culture was attempted on 178 miscarriages. Karyotyped miscarriages served as controls in this blinded study. Results were confirmed by quantitative fluorescent-PCR (QF,PCR). Results Among the karyotyped miscarriages, MLPA was able to detect all the expected aneuploidies, as well as an unbalanced product from a reciprocal translocation, and revealed cryptic deletions and duplications not visible at the 550-band resolution level. In addition, chromosomal anomalies were found in ,37% of cases that failed to grow or could not be cultivated. As expected, ploidy changes were not detected. Copy number variation was found for target sequences of P036B (CYFIP1, MRPL41, CAB45) and P070 (DECR2, TNFRSF18) probe mixes. Conclusions We propose the use of MLPA with subtelomeric probe mixes as a reliable, rapid and economical first approach to detect aneuploidy and unbalanced terminal chromosomal rearrangements in SAs. Copyright © 2007 John Wiley & Sons, Ltd. [source] Social capital and information science researchPROCEEDINGS OF THE AMERICAN SOCIETY FOR INFORMATION SCIENCE & TECHNOLOGY (ELECTRONIC), Issue 1 2007Catherine A. Johnson (moderator, presenter) The concept of social capital has become a popular area of research in many social science fields, including public policy, political science, economics, community development, sociology, anthropology, and education. Increasingly, it has been used as the conceptual framework for research in the area of information studies including such topics as knowledge integration (Bhandar, Pan & Tan, 2007), knowledge sharing (Huysman & Wulf, 2006), access to information by the homeless (Hersberger, 2003), community informatics (Williams and Durrance, in press), and information seeking behavior (Johnson, in press). The concept has an ideological foundation in the theories of Pierre Bourdieu (1980), with two divergent approaches to its study emerging during the last two decades: one focusing on social capital as a collective asset and the other regarding it as an individual asset. The main proponent of the first approach is political scientist Robert Putnam who defines social capital as inhering in the "dense networks of social interaction" which foster "sturdy norms of generalized reciprocity and encourage the emergence of social trust" (Putnam, 1995, p. 66). Social network analysts, on the other hand, view social capital as resources to which individuals have access through their social relationships. Nan Lin, who is the main proponent of this approach, defines social capital as "resources embedded in a social structure which are accessed and/or mobilized in purposive actions" (Lin, 2001a, p. 12). While the concept of social capital may be operationalized differently depending on the point of view of the researcher, its value to information science research is in providing a framework within which to understand the relationship between social structure and information access. Participants in this panel will discuss social capital from various vantage points, including the role of social capital in solidifying power relationships, the effect of recent government policies on reducing social capital, and the relationship between social capital and the use of libraries and information technology. The intent of the panel is to clarify the meaning(s) of social capital and to demonstrate how the concept may be used in information science research. [source] Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharidesPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 14 2008Johannes Stadlmann Abstract Two LC-ESI-MS methods for the analysis of antibody glycosylation are presented. In the first approach, tryptic glycopeptides are separated by RP chromatography and analyzed by ESI-MS. This "glycopeptide strategy" allows a protein- and subclass-specific quantitation of both neutral and sialylated glycan structures. Additional information about under- or deglycosylation and the protein backbone, e.g., termini, can be extracted from the same data. In the second LC-ESI-MS method, released oligosaccharides are separated on porous graphitic carbon (PGC). A complete structural assignment of neutral and sialylated oligosaccharides occurring on antibodies is thereby achieved in one chromatographic run. The two methods were applied to polyclonal human IgG, to commercial mAb expressed in CHO cells (Rituximab, Xolair, and Herceptin), in SP2/0 (Erbitux and Remicade) or NS0 cells (Zenapax) and the anti-HIV antibody 4E10 produced either in CHO cells or in a human cell line. Both methods require comparably little sample preparation and can be applied to SDS-PAGE bands. They both outperform non-MS methods in terms of reliability of peak assignment and MALDI-MS of underivatized glycans with regard to the recording of sialylated structures. Regarding fast and yet detailed structural assignment, LC-MS on graphitic carbon supersedes all other current methods. [source] Linkage disequilibrium patterns and genetic structure of Amerindian and non-Amerindian Brazilian populations revealed by long-range X-STR markersAMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 3 2009Fabio P.N. Leite Abstract The extent of X-chromosome linkage disequilibrium (LD) was studied in a southern Brazilian population, and in a pool of samples from Amerindian populations. For this purpose, 11 microsatellites, located mostly in a Xq region comprising ,86 Mb was investigated. The lower Amerindian gene diversity associated with significant differences between the populations studied indicated population structure as the main cause for the higher LD values in the Amerindian pool. On the other hand, the LD levels of the non-Amerindian Brazilian sample, although less extensive than that of the Amerindians, were probably determined by admixture events. Our results indicated that different demographic histories have significant effects on LD levels of human populations, and provide a first approach to the X-chromosome ancestry of Amerindian and non-Amerindian Brazilian populations, being valuable for future studies involving mapping and population genetic studies. 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