Home  About us  Contact
 

Alkaloid Extract (alkaloid + extract)

Distribution by Scientific Domains
Chemistry66%

Selected Abstracts

Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts

PHYTOCHEMICAL ANALYSIS, Issue 5 2008
Strahil Berkov
Abstract Alkaloid extracts from 12 plant species of the families Amaryllidaceae, Fumariacae and Papaveraceae were studied with respect to their acetylcholinesterase inhibitory activity and alkaloid patterns. Fifty-three alkaloids were identified by GC-MS, including known acetylcholinesterase (AChE) inhibitors such as galanthamine, epigalanthamine, sanguinine and epinorgalanthamine in extracts of Amaryllidaceae plants and protopine in extracts of Fumariaceae and Papaveraceae plants. The galanthamine-containing extracts of the amaryllidaceous plants were found to be the most active while the extract of Corydalis bulbosa was the most active among the extracts of the tested plants from the Fumariaceae and Papaveraceae plants. TLC bioautographic assay, preparative TLC and GC-MS analysis were combined to identify the active compounds in the studied extracts. Galanthamine was isolated from the known AChE inhibitors in the extracts of Amaryllidaceae plants. Corydaline, bulbocapnine and stylopine were found to be active in the extracts of plant species of the families Fumariaceae and Papaveraceae. Available standards of deshydrocorydaline,a precursor of corydaline, corydaline and stylopine,were tested for AChE inhibitory activity. Deshydrocorydaline and corydaline showed potent inhibitory activity comparable with that of the positive control galanthamine. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Activity of Zanthoxylum clava-herculis extracts against multi-drug resistant methicillin-resistant Staphylococcus aureus (mdr-MRSA)

PHYTOTHERAPY RESEARCH, Issue 3 2003
Simon Gibbons
Abstract In a continuing search for compounds with antibiotic activity against methicillin-resistant Staphylococcus aureus (MRSA) possessing multidrug ef,ux systems, we have demonstrated activity associated with extracts from Southern prickly ash bark, Zanthoxylum clava-herculis. Bioassay-guided isolation of an alkaloid extract led to the characterization of the benzo[c]phenanthridine alkaloid chelerythrine as the major active principle. This compound exhibited potent activity against strains of MRSA, which were highly resistant to clinically useful antibiotics via multidrug ef,ux mechanisms. Copyright © 2003 John Wiley & Sons, Ltd. [source]

Quaternary benzo[c]phenanthridine alkaloids as inhibitors of aminopeptidase N and dipeptidyl peptidase IV

PHYTOTHERAPY RESEARCH, Issue 1 2002
Aleksi
Abstract Chelerythrine, sanguinarine and an alkaloid extract from Macleaya cordata,sanguiritrin,were found to be inhibitors of aminopeptidase A and dipeptidyl peptidase IV, while fagaronine inhibited dipeptidyl peptidase IV only. At 50,,M, chelerythrine, sanguinarine and sanguiritrin inhibited aminopeptidase N by 82%, 82%, 88%, DPP IV by 38%, 62%, 57%, and fagaronine by 34%, respectively. When bovine serum albumin (500,,g/mL) was added, the inhibition of both proteases by quaternary benzo[c]phenanthridine alkaloids (QBA) (50,,M) was significantly diminished. Strong interaction of chelerythrine and sanguinarine with bovine and human serum albumin was proved by electrophoretic determination of their respective conditional binding constants. Copyright © 2002 John Wiley & Sons, Ltd. [source]

LC-MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

BIOMEDICAL CHROMATOGRAPHY, Issue 4 2009
Jiang-Feng Yuan
Abstract A novel method of cell affinity screening (CAS), cell affinity capture coupled with LC-MS analysis, was developed for screening the bioactive compounds related to cardiovascular diseases from the natural product libraries. One of the major characteristics lies in its function in affinity-capturing and separating the bioactive components from the natural product libraries in vitro. Another characteristic is its use in analyzing and identifying the target compounds, by employing high-performance liquid chromatography and mass spectrometry. CAS was used for screening the bioactive components from the alkaloid extract derived from Aconitum szechenyianum Gay. Of the five components found to be bound to the oxidative-damaged endothelial cells, the two compounds identified, mesaconitine and aconitine, were recognized in the literature as being related to cardiovascular diseases. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts

PHYTOCHEMICAL ANALYSIS, Issue 5 2008
Strahil Berkov
Abstract Alkaloid extracts from 12 plant species of the families Amaryllidaceae, Fumariacae and Papaveraceae were studied with respect to their acetylcholinesterase inhibitory activity and alkaloid patterns. Fifty-three alkaloids were identified by GC-MS, including known acetylcholinesterase (AChE) inhibitors such as galanthamine, epigalanthamine, sanguinine and epinorgalanthamine in extracts of Amaryllidaceae plants and protopine in extracts of Fumariaceae and Papaveraceae plants. The galanthamine-containing extracts of the amaryllidaceous plants were found to be the most active while the extract of Corydalis bulbosa was the most active among the extracts of the tested plants from the Fumariaceae and Papaveraceae plants. TLC bioautographic assay, preparative TLC and GC-MS analysis were combined to identify the active compounds in the studied extracts. Galanthamine was isolated from the known AChE inhibitors in the extracts of Amaryllidaceae plants. Corydaline, bulbocapnine and stylopine were found to be active in the extracts of plant species of the families Fumariaceae and Papaveraceae. Available standards of deshydrocorydaline,a precursor of corydaline, corydaline and stylopine,were tested for AChE inhibitory activity. Deshydrocorydaline and corydaline showed potent inhibitory activity comparable with that of the positive control galanthamine. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Simultaneous determination of peimine and peiminine in rat plasma by LC-ESI-MS employing solid-phase extraction

BIOMEDICAL CHROMATOGRAPHY, Issue 8 2010
Xiaodan Wu
Abstract A simple and reliable LC-ESI-MS method for the determination of peimine and peiminine in rat plasma was developed for the first time. The method was proven to be specific and sensitive by carrying out validation. The analytes were extracted from rat plasma via solid-phase extraction on Waters Oasis MCX cartridges. Chromatography separation was achieved on a C18 column using 10,mM ammonium acetate (adjusted to pH 3.0 with glacial acetic acid),acetonitrile (85:15, v/v) as mobile phase. The linear range was 1,100,ng/mL for peimine and peiminine. Intra- and inter-day precisiond were less than 10%. Accuracies were within 85,115% of their nominal concentrations. The limit of quantification was 1,ng/mL for both analytes. The developed assay was successfully applied to pharmacokinetic study of peimine and peiminine in rats orally administered the alkaloids extracts from Bulbus Fritillariae, demonstrating a possible broader spectrum of applications of this method. Copyright © 2009 John Wiley & Sons, Ltd. [source]