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Albino Rabbits (albino + rabbits)
Kinds of Albino Rabbits Selected AbstractsThe use of plasma rich in growth factors on wound healing in the skin: experimental study in rabbitsINTERNATIONAL WOUND JOURNAL, Issue 2 2009Francesco Molina-Mińano ABSTRACT The objective of this study was to use autologous plasma rich in growth factors (PRGF) on wound healing in the skin in New Zealand albino rabbits and to study reepithelialisation and inflammation at 7 and 28 days. A prospective study carried out on 20 adult rabbits. Two wounds were made on the in the skin on the back of each animal; one control, and the other in which PRGF was applied. The PRGF preparation was obtained from 10 ml of whole blood. The reepithelialisation and inflammation of wounds were measured at 7 and 28 days. Reepithelialisation improved in skin at 7 days (P = 0·007), with resolution of the inflammatory process (P = 0·005), having significant differences with respect to the control. Therefore, PRGF accelerates reepithelialisation and reduces inflammation at 7 days in skin. [source] Free radical scavengers are more effective than indomethacin in the prevention of experimentally induced heterotopic ossificationJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 2 2007L.C. Vanden Bossche Abstract The pathogenesis of heterotopic ossification is still unclear and the preventive therapies are usually insufficient. The present study was designed to investigate the possible preventive effect of free radical scavengers on the development of experimentally induced heterotopic ossification in a rabbit model and to compare free radical scavengers with indomethacin to determine whether they act synergistically. A standard immobilization,manipulation model was used to induce heterotopical ossification in the hind legs of 40 1-year-old female New Zealand albino rabbits. The animals were divided into four groups and received daily either placebo, a free radical scavenger cocktail [allopurinol and N -acetylcysteine (A/A)], indomethacin or the combination of A/A and indomethacin in a randomized double-blind fashion. Every 4 days an X-ray was taken and the thickness and length of new bone formation was measured at the thigh. A marked statistically significant difference was found between the four groups. In the groups that received A/A, either alone or combined with indomethacin, an inhibition of bone growth, both in thickness and in length was demonstrated. In this experimental model free radical scavengers had a superior inhibitory effect on heterotopic ossification than indomethacin. Free radicals could play an important role in the pathogenesis of heterotopic ossification. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:267,272, 2007 [source] Effect of Two Different UVA Doses on the Rabbit Cornea and LensPHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 3 2009ejka, estmír The aim of the present paper was to examine the irradiation effect of two doses of UVA rays (365 nm) on the rabbit cornea and lens. Corneas of anesthetized adult albino rabbits were irradiated with UVA rays for 5 days (daily dose 1.01 J cm,2 in one group of rabbits and daily dose 2.02 J cm,2 in the second group of animals). The third day after the last irradiation, the rabbits were killed, and their eyes were employed for spectrophotometrical, biochemical and immunohistochemical investigations. Normal eyes served as controls. Absorption spectra of the whole corneal centers were recorded over the UV,VIS (visible) spectral range. Levels of antioxidant and prooxidant enzymes, nitric oxide synthases and nitric oxide (indirectly measured as nitrate concentration) were investigated in the cornea. Malondialdehyde, a byproduct of lipid peroxidation, was examined in the cornea and lens. The results show that the staining for endothelial nitric oxide synthase was more pronounced in corneas irradiated with the higher UVA dose. Otherwise, UVA rays at either dose did not significantly change corneal light absorption properties and did not cause statistically significant metabolic changes in the cornea or lens. In conclusion, UVA rays at the employed doses did not evoke harmful effects in the cornea or lens. [source] Effect of caffeic acid phenethyl ester on treatment of experimentally induced methicillin-resi,stant Staphylococcus epidermidis endophthalmitis in a rabbit modelCELL BIOCHEMISTRY AND FUNCTION, Issue 6 2007Özlem Y Abstract This study investigated the anti-inflammatory effects of caffeic acid phenethyl ester (CAPE), a natural bee-produced compound, and compared it with corticosteroids in the treatment of experimentally induced methicillin-resistant Staphylococcus epidermidis (MRSE) endophthalmitis in addition to intravitreal antibiotics. An experimental endophthalmitis model was produced in 24 New Zealand albino rabbits by unilateral intravitreal injection of 0.1,ml of 4.7,×,104 colony-forming units (CFU) methicillin-resistant S. epidermidis. The animals were then divided randomly into three treatment groups and a control group, group 1 (six rabbits), received only intravitreal vancomycin (1.0,mg/0.1,ml); group 2 (six rabbits), received both intravitreal vancomycin (1.0,mg/0.1,ml) and intravitreal dexamethasone (400,µg/0.1,ml) and group 3 (six rabbits), received both intravitreal vancomycin (1.0,mg/0.1,ml) and subtenon CAPE (10,mg/0.3,ml) after 24,h post-infection. No treatment was given to the control group. Treatment efficacy was assessed by clinical examination, vitreous culture and histopathology. There were no statististically significant differences between clinical scores of all groups in examinations at 24 and 48,h post-infection (p,=,0.915 and p,=,0.067 respectively), but in examinations at 72,h post-infection and after 7 days post-infection, although the clinical scores of treatment groups were not significantly different from each other, they were significantly lower than the control group (p,<,0.05). The culture results of all groups were sterile. As a result, CAPE was found to be as effective as dexamethasone in reducing inflammation in the treatment of experimental MRSE endophthalmitis when used with antibiotics. More studies are needed to determine the optimal administration route and effective dosage of this compound. Copyright © 2006 John Wiley & Sons, Ltd. [source] Intravitreal and plasmatic levels of erythropoietin after sub-conjunctival administration in rabbitsACTA OPHTHALMOLOGICA, Issue 2009AP RESENDE Purpose Recently Erythropoietin (EPO) had been shown to have neuroprotective and neuroregenerative effects on retinal ganglion cells, apart from its erythropoietic properties, being a promissory alternative on ischemic retinal diseases. With the present study we pretend to evaluate the efficacy of subconjuntival injection for ocular EPO delivery. Methods New Zealand albino rabbits (n=6) were used. Complete ophthalmic examinations were carried out before and after the injections for 15 days. The proceedings (intravitreal punch and subconjuntival injections) were carried out under general anaesthesia. Through the subconjuntival route 100 UI of EPO diluted in 50 ,l saline solution 0,9% was administered. The opposite eyes of each animal served as controls. The vitreous and plasmatic concentration of EPO were measured using the ELISA method. Results Administration of EPO through the subconjuntival route allowed a vitreous absortion that reached the highest 24 hours after administration with 0,6 mUI/ml of EPO quantified in 100 ,l of vitreous sample. The sistemic absorption reached the highest concentration 3 hours after subconjunctival administration and 48 hours after the administration the plasmatic concentration of EPO regained physiologic values. EPO was not detected on control eyes. Conclusion All the previous studies used the systemic or intravitreal route of administration to acquire therapeutic concentrations of EPO on the retina, both difficult to use in clinical practice. In this study, the subconjuntival route proved to be a promising alternative for ocular EPO delivery. However further studies are necessary to assess the blood and intravitreal kinetics of EPO after subconjuntival administration. [source] Lornoxicam pharmacokinetics in the vitreous humor of albino rabbitsACTA OPHTHALMOLOGICA, Issue 2009C TSIKA Purpose To assess the elimination half-life of intravitreal lornoxicam, a non-steroidal anti-inflammatory drug (NSAID). Methods Both eyes of 15 rabbits were intravitreally injected with 250 ,g of commercially available lornoxicam (for intravenous/intramuscular use, Xefo® 8 IV/IM Injection, Nycomed Hellas S.A.). Six eyes were enucleated at time points 0, 1, 2, 6 and 24 hours after the injection was performed. The eyes were immediately frozen at -80°C. The vitreous was eviscerated from the eye and the drug was liquid-liquid extracted from a 0.4 ml sample. Lornoxicam was isolated by a reversed-phase High Performance Liquid Chromatography (HPLC) method at retention time 10.7 min and detected at 372 nm. The data were statistically analyzed in order to evaluate the pharmacokinetic parameters of the drug. Results The recovery of lornoxicam after liquid-liquid extraction was calculated at 69.6% and the limit of determination was 0.1 ,g/ml. Statistical analysis revealed that lornoxicam concentrations followed first-order kinetics with an elimination rate constant of 0.235h-1 and a half-life of 3.0 h. Conclusion The determination of the pharmacokinetic characteristics of intravitreal lornoxicam allows the possibility for further investigation of the drug's intraocular behaviour and therapeutic potential. [source] Cyclosporine treatment of RPE allografts in the rabbit subretinal spaceACTA OPHTHALMOLOGICA, Issue 2 2000Sven Crafoord ABSTRACT. Purpose: To determine the effects of systemic cyclosporine A (CsA) on the survival of retinal pigment epithelial (RPE) allografts in the subretinal space in an animal model using atraumatic transplantation surgery. Methods: Following pars plana vitrectomy, an RPE cell suspension from brown rabbits was injected with a glass micropipette into the subretinal space of 39 albino rabbits. For immunosuppression, 22 rabbits were given an injection of CsA, 20 mg daily intramuscularly, 17 rabbits with RPE grafts were controls. The grafts were monitored by biomicroscopy, color fundus photography, and fluorescein angiography. Rabbits were sacrificed at 1, 3 and 6 months, respectively, and the eyes processed for light and electron microscopy including immunohistochemistry. Results: After three months, the transplanted RPE cells, in both the CsA group and the controls, formed a monolayer in the subretinal space. Although a few macrophages were encountered, there was no massive cellular infiltration and the photoreceptor layer was well preserved. After six months, however, there was a disruption of grafted RPE cells in both groups, characterized by dispersion of melanin pigment in the subretinal space, and invasion of macrophages with focal photoreceptor damage but no infiltration of lymphocytes in the retina or choroid. No significant differences between the CsA treated and the control eyes were discernible. Conclusion: Although the subretinal space has been considered an immunologically privileged site, we found that the survival of RPE allografts was limited. CsA did not prevent RPE allograft destruction in the subretinal space. The transplant seems to be disrupted either by immunological mechanisms that are not inhibited by CsA, or by nonimmunologic events. [source] Dissection and cotransplantation of large pieces of RPE and neural retina; effect of protease K on the developmentACTA OPHTHALMOLOGICA, Issue 1 2000Rajesh Kumar Sharma ABSTRACT. Purpose: This study attempts to cotransplant large pieces of the RPE and neural retina in the subretinal space of rabbits by using protease K for dissection of the donor tissue, and to investigate the effect of dissection technique on the development of the grafts. Methods: Eyes from 15-day-old pigmented rabbit embryos were partly digested by protease K to assist dissection of sclera and the choroid from RPE and neural retina. Large pieces of RPE and the neural retina thus obtained were cotransplanted into the eyes of adult albino rabbits who were allowed to survive for up to 63 days. The transplants were examined under light microscope. Results: It was possible to transplant large sheets of RPE and neural retina together. Both the RPE and the neural retina survived after cotransplantation. Retinal pigment epithelium survived in layers, but at places formed clusters. In cotransplants neural retina formed rosettes, developed gliosis, and photoreceptors failed to develop outer segments, possibly due to the action of protease K. Conclusion: Proteases seem to be injurious for the development of the neural retina. [source] | |||||||||||||