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Field Cages (field + cage)
Selected AbstractsThe nature and reality of the aphid clone: genetic variation, adaptation and evolutionAGRICULTURAL AND FOREST ENTOMOLOGY, Issue 2 2008Hugh D. Loxdale Abstract 1,When aphid clones and clonality are discussed, it is still often said that they are ,genetically identical', a statement for which there is no direct evidence, and certainly not for the entire genome. By contrast, there is a growing body of empirical data from the application of high resolution molecular (DNA) markers that aphid asexual lineages rapidly mutate and that, in some documented cases, this variation is selectable, either positively or negatively. 2,Although it is true that, in enclosed conditions (e.g. laboratory or field cage), a so-called clone as defined as the asexual progeny of a single foundress may be traceable, this is rarely if ever possible in the field without the use of genetic markers, and even then, usually only at a relatively few loci (multilocus genotypes, ,MLGs'). 3,The continued use of the term clone without qualification of its true nature and the reality of its interesting biology is likely to hamper a proper understanding of the ecology and evolution of these insects (which are interesting in their own right because of their complex life histories, but also because they are important as major pests globally, both by causing direct feeding damage and by transmitting pathogenic plant viruses and thereby leading to huge economic losses in the agricultural, horticultural and forestry industries). 4,In this short review, I provide evidence of what is now known about aphid clonality after the widespread use of molecular markers, comprising information mainly gained within the last 15 years or so. 5,The data demonstrate widespread adaptation and evolution, sometimes involving introgression and hybridization. Because of this new knowledge, our ideas of what constitutes a clone are in need of serious re-evaluation. [source] Establishment in Australia of Trichopoda giacomellii (Blanchard) (Diptera: Tachinidae), a biological control agent for Nezara viridula (L.) (Hemiptera: Pentatomidae)AUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 3 2000Marc Coombs Abstract Releases of Trichopoda giacomellii (Blanchard) for biological control of Nezara viridula (L.) took place at sites in western New South Wales and south-eastern Queensland between 1996 and 1999. Trichopoda giacomellii became established near Moree in north-western New South Wales, whereas establishment at sites near Brisbane has not been confirmed. Releases of adult T. giacomellii and of parasitised N. viridula were made either directly onto host plants (1996 and 1997) or following temporary confinement in a field cage (1998 and 1999). Parasitism of N. viridula adults by T. giacomellii ranged from 9 to 72% between January 1999 and February 2000, and was 42% of adults during diapause. The methods employed to establish T. giacomellii in this study are compared with other attempts to establish Trichopoda spp. elsewhere in the world. Release methodologies that limit immediate dispersal of the agent and/or their progeny are recommended for establishing Trichopoda spp. [source] Adult mortality and oviposition rates in field and captive populations of the blowfly Lucilia sericataECOLOGICAL ENTOMOLOGY, Issue 6 2004K. M. Pitts Abstract., 1. Adult mortality and oviposition rates were determined for populations of the blowfly Lucilia sericata (Meigen) (Diptera: Calliphoridae). This species is of economic importance as the primary agent of sheep myiasis throughout north-western Europe. 2. Populations of marked flies in six, 1 m3, outdoor field cages and unmarked wild flies at two farms in south-west England were studied simultaneously between May and September 1998. 3. In the field, wild female L. sericata were caught and aged using a combination of wing-fray and ovarian dissection techniques. Survivorship analysis gave estimates of mortality of 1.94% (± 0.037) and 2.09% (± 0.044) per day-degree and mean life expectancy of 51.5 and 47.9 day-degrees above a threshold of 11 °C, at the two farms studied. Mean lifetime reproductive output in the field was estimated to be 159.6 and 138.4 eggs per female at the two farms respectively. 4. The survivorship of cohorts of marked female flies in cages was followed by counting the number of dead individuals each day; the mortality rate of these flies was 0.81% per day-degree (± 3.49 × 10,4%) and the mean life expectancy was 123.1 day-degrees above a threshold of 11 °C. Mortality rate was shown to increase significantly with average ambient temperature and relative humidity lagged for two sample periods (approximately 10 days). Oviposition rate also increased with average temperature but declined with average relative humidity. A best-fit multiple regression model incorporating both ambient temperature and humidity explained 60.5% of the variance in the pattern of oviposition. 5. The differences between the field and cage populations highlight the caution required when extrapolating life-history parameters from artificial to natural habitats. [source] Methoprene modulates the effect of diet on male melon fly, Bactrocera cucurbitae, performance at mating aggregationsENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 1 2010Ihsan ul Haq Abstract The effect of access to dietary protein (P) (hydrolyzed yeast) and/or treatment with a juvenile hormone analogue, methoprene (M), (in addition to sugar and water) on male aggregation (lekking) behaviour and mating success was studied in a laboratory strain of the melon fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). Six-day-old males were treated with (1) protein and methoprene (M+P+), (2) only protein (M,P+), or (3) only methoprene (M+P,), and compared with 14-day-old sexually mature untreated males (M,P,). The lekking behaviour of the four groups of males when competing for virgin sexually mature females (14,,16 days old) was observed in field cages. The following parameters were measured at male aggregations: lek initiation, lek participation, males calling, male,male interaction, female acceptance index, and mating success. For all these parameters, the M+P+ males significantly outperformed the other males. Moreover, for all parameters, there was a similar trend with M+P+ > M,P+ > M,P, > M+P,. More M+P+ males called and initiated and participated in lek activities than all other types of male, which resulted in higher mating success. They had also fewer unsuccessful copulation attempts than their counterparts. Whereas treatment with methoprene alone had a negative effect in young males with only access to sugar, access to dietary protein alone significantly improved young male sexual performance; moreover, the provision of methoprene together with protein had a synergistic effect, improving further male performance at leks. The results are of great relevance for enhancing the application of the sterile insect technique (SIT) against this pest species. The fact that access to dietary protein and treatment of sterile males with methoprene improves mating success means that SIT cost-effectiveness is increased, as more released males survive to sexual maturity. [source] Floral resources impact longevity and oviposition rate of a parasitoid in the fieldJOURNAL OF ANIMAL ECOLOGY, Issue 3 2008Jana C. Lee Summary 1The use of floral resource subsidies to improve herbivore suppression by parasitoids requires certain trophic interactions and physiological changes to occur. While the longevity and fecundity of parasitoids are positively affected by nectar subsidies in laboratory studies, the impacts of floral subsidies on the fecundity and longevity of freely foraging parasitoids have not been studied. 2We studied the longevity and per capita fecundity of naturally occurring Diadegma insulare foraging in cabbage plots with and without borders of flowering buckwheat, Fagopyrum esculentum, as well as relationships between longevity, fecundity, sugar feeding and parasitism rates on larvae of the diamondback moth, Plutella xylostella. 3Relative longevity was estimated by counting broken setae on the fringe of the forewing. Floral borders increased the longevity of males and females in adjacent cabbage plots. 4The egg maturation rate of D. insulare was estimated by comparing egg loads of females collected early in the day with egg loads of females held without hosts in field cages throughout the day. Females in buckwheat cages matured 2·7 eggs per hour while females in control cages resorbed 0·27 eggs over the same time period. 5The fecundity of females collected in the afternoon was estimated by comparing their actual egg load to the estimated egg load in the absence of oviposition for females in a given plot. Females foraging in buckwheat plots had marginally fewer eggs remaining in their ovaries, and laid marginally more eggs than females in control plots. Females from both treatments carried 30,60 eggs by the afternoon and therefore were time-limited rather than egg-limited. 6Plots where a greater proportion of females had fed on sugar had longer-lived females. This suggests that feeding enhanced longevity of D. insulare. However, plots with longer-lived and more fecund females did not exhibit higher parasitism rates, although the power of these tests were low. [source] Non-target habitat exploitation by Trichogramma brassicae (Hym. Trichogrammatidae): what are the risks for endemic butterflies?AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 3 2003D. Babendreier Abstract 1,Trichogramma brassicae Bezdenko is inundatively released against the European corn borer in Switzerland. Because parasitoids dispersing from the release fields might pose a threat to native butterflies, the searching efficiency of T. brassicae was investigated in nontarget habitats. 2,In field studies, T. brassicae was released at rates of 120 000 females/ha. Parasitism of sentinel Ephestia kuehniella egg clusters was 1.6,3.6% in meadows and 2.0,4.0% in flower strips. The respective figures were 57.6,66.7% and 19.2,46.9% in maize, significantly higher than the parasitism rates in the nontarget habitats. Experiments carried out in small field cages confirmed these results: Again, significantly higher parasitism rates were found in maize compared to meadows and flower strips, and also compared to hedgerows (in sleeve cages). 3,To elucidate potential factors underlying the low searching efficiency in nontarget habitats, the behaviour of individual T. brassicae females was investigated on four meadow plants comparatively to maize and a filter paper control. Mean (±SE) walking speed on maize was 2.2 ± 0.2 mm/s, similar to three of the plants tested and filter paper but significantly higher than on Trifolium pratense (0.85 mm/s). A higher turning rate was found on T. pratense, Viola wittrockiana and Plantago lanceolata, in contrast to the longer leaved maize and Alopecurus pratensis. The number of wasps leaving the plant within the observation period differed significantly between plant species, and was twice as high for T. pratense (and the filter paper control) compared to the other plant species. 4,In a choice experiment carried out in a climate cabinet with all five host plant species in cages, we obtained the highest parasitism rates on maize and the lowest parasitism on T. pratense, thus confirming the behavioural observations. 5,In conclusion, there is evidence for a decreased searching efficiency on plants in nontarget habitats compared to maize. However, the data explain only part of the differences found between parasitism in maize compared to nontarget habitats. Other factors, such as the structural complexity of a habitat, may also play a role. We conclude that the risk for butterfly populations in the tested nontarget habitat due to mass released T. brassicae is low. [source] Seasonal synchrony of life cycles between the exotic predator, Pseudoscymnus tsugae (Coleoptera: Coccinellidae) and its prey, the hemlock woolly adelgid Adelges tsugae (Homoptera:Adelgidae)AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 4 2000Carole A. S. -J. Abstract 1 The seasonal synchrony between the exotic predator, Pseudoscymnus tsugae and its prey, the hemlock woolly adelgid, Adelges tsugae, was investigated in field cages and in the forest in Connecticut, U.S.A. from 1997,1999. 2 In early spring, egg to adult development took 45 d at 18.7 °C, 39.7 d at 20.2 °C and 31.5 d at 22.7 °C. Earliest emerging F1 adults mated and oviposited in the same year. whereas F1 and F2 females emerging later in the summer mated and reserved most of their egg complement for the following year. 3 A second generation of P. tsugae is possible in Connecticut but may be delayed by cool mid-spring temperatures. Individuals of three generations of P. tsugae, including overwintering survivors, may coexist in July and August and adults can be found year-round with A. tsugae in infested hemlock forests. 4 A linear regression model for development from egg to adult under field temperatures gave good agreement with results from constant temperature findings. The model predicted a lower development threshold of 9.5 °C and a sum of effective temperatures of 405 day °C. Development time of P. tsugae is shorter relative to its prey A. tsugae and generation time ratios of predator to prey was 0.16,0.5, with an advantage conferred on the coccinellid. 5 Overwintering ability and behaviour were determined in 1998,1999 and adults remained on infested hemlock branches throughout a mild winter, becoming reproductively active in mid-April. Peak oviposition period extended from April to July, in synchrony with peak oviposition and developing stages of two generations of A. tsugae. [source] | ||||||||||