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Fatty Acid Concentrations (fatty + acid_concentration)
Kinds of Fatty Acid Concentrations Selected AbstractsDecreased polyunsaturated and increased saturated fatty acid concentration in spermatozoa from asthenozoospermic males as compared with normozoospermic malesANDROLOGIA, Issue 5 2006H. Tavilani Summary The lipid composition of the sperm membrane has been shown to exert a significant effect upon the functional quality of spermatozoa. We have studied fatty acid composition of the phospholipids in spermatozoa in asthenozoospermic and normozoospermic men and determined the ratio of polyunsaturated fatty acids (PUFAs) to saturated fatty acids of spermatozoa of these two groups. Fatty acid concentration of spermatozoa was determined in 15 asthenozoospermic and eight normozoospermic semen samples by thin layer chromatography and gas chromatography. The most abundant polyunsaturated and saturated fatty acids in normozoospermic samples were docosahexaenoic acid (DHA 22 : 6 ,3, 98.5 ± 4.5 nmol per 108 spermatozoa, mean ± SE) and palmitic acid (103 ± 17 nmol per 108 spermatozoa) respectively. The mean ± SE values of DHA and palmitic acid in asthenozoospermic samples were 53.9 ± 11.6 and 145 ± 14.7 nmol per 108 spermatozoa respectively. Compared with normozoospermic samples, asthenozoospermic samples showed lower levels of PUFA and higher amount of saturated fatty acids. The mean ± SE ratios of sperm PUFA/saturated fatty acids in asthenozoospermic and normozoospermic samples were 0.66 ± 0.06 and 1.45 ± 0.16 (P < 0.001) respectively. This study demonstrates that spermatozoa of asthenozoospermic men have lower levels of PUFA compared with saturated fatty acids. This may be contributory to the poor motility noted in samples from these men. [source] Effect of different liquid cultures of live yeast strains on performance, ruminal fermentation and microbial protein synthesis in lambsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 6 2008M. K. Tripathi Summary Three yeast strains, Kluyveromyces marximanus NRRL-3234 (KM), Saccharomyces cerevisiae NCDC-42 (SC) and Saccharomyces uvarum ATCC-9080 (SU), and a mixed culture (1:1:1 ratio) were evaluated for their value as probiotics in lamb feeding in two experiment. In experiment I and II, 20 and 30 pre-weaner lambs were fed for 63 and 60 days in two and three equal groups respectively. All lambs were offered ad libitum a creep mixture and Zizyphus nummularia leaves, and yeasts were dosed orally. In experiment I, one group received no yeast, the other of the mixed culture (1.5,2 × 1010 live cells/ml). In experiment II, yeast cultivation was modified yielding 1.5,2 × 1013 live cells/ml. Lambs of the three experimental groups received 1 ml/kg live weight of one of the individual yeasts. Feed intake did not differ among groups of both experiments with the exception of SC-supplemented lambs in experiment II which showed a trend to higher intakes per kg metabolic body weight and in percentage of body weight when compared with KM- and SU-supplemented lambs. Supplementation of the mixed yeast culture had no effect on intakes of digestible crude protein and metabolisable energy, nutrient digestibility, nitrogen balance and rumen fermentation characteristics (pH, ammonia, volatile fatty acid concentration, protozoa count) and urinary allantoin as an indicator of microbial protein synthesis. The same was true for comparisons in experiment II except ciliate protozoa counts, which showed a trend to be the highest with SU and the lowest with SC. The results of present study show that the response of lambs to supplemented live yeast cultures is inconsistent, as it lacked to have an effect in the present study, and that differences among strains were small, even when supplemented at a much higher live cell count. [source] Chemical Modification of Pigskin Gelatin: Factors Affecting the Esterification of Gelatin with Fatty AcidJOURNAL OF FOOD SCIENCE, Issue 9 2001K.B. Djagny ABSTRACT: This study investigated the esterification of pigskin gelatin with fatty acid catalyzed by acid in aqueous medium. Factors affecting the esterification reaction B temperature, pH, water content, fatty acid concentration, fatty acid type and reaction time- were elucidated in the view of optimizing the reaction. Under the same experimental conditions, increase in fatty acid concentration permitted the determination of the maximum amount of fatty acid that could be esterified per unit weight of gelatin and demonstrated that not all the hydroxyl functional groups present in the gelatin could be blocked by the fatty acid chains. [source] Plasma free fatty acid profiling in a fish oil human intervention study using ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometryRAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 13 2008Nicole Zehethofer A rapid method was developed for the simultaneous profiling of 29 free fatty acids in plasma using ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS). Barium acetate was used as the cationization agent in the positive ion mode for sensitive multiple reaction monitoring (MRM) experiments. The cis- and trans -C18:1 and -C18:2 isomers were baseline-separated using two tandem reversed-phase C18 UPLC columns, while identification of two pairs of positional isomers of C18:3 and C20:3 required isomer-specific product ions, as the analytes were not chromatographically resolved. The assay linearity was greater than three orders of magnitude and correlation coefficients were >0.99; the limits of detections were typically less than 0.2,µM. The method was successfully applied to plasma free fatty acid profiling of samples from volunteers who participated in a randomized crossover study involving the administration of either placebo or fish oil capsules. The results clearly indicate the ability to measure the time profiles of the n -3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in plasma for the volunteers given fish oil capsules while the concentrations of the other free fatty acids and the total free fatty acid concentration in plasma remained virtually constant. Copyright © 2008 John Wiley & Sons, Ltd. [source] Decreased polyunsaturated and increased saturated fatty acid concentration in spermatozoa from asthenozoospermic males as compared with normozoospermic malesANDROLOGIA, Issue 5 2006H. Tavilani Summary The lipid composition of the sperm membrane has been shown to exert a significant effect upon the functional quality of spermatozoa. We have studied fatty acid composition of the phospholipids in spermatozoa in asthenozoospermic and normozoospermic men and determined the ratio of polyunsaturated fatty acids (PUFAs) to saturated fatty acids of spermatozoa of these two groups. Fatty acid concentration of spermatozoa was determined in 15 asthenozoospermic and eight normozoospermic semen samples by thin layer chromatography and gas chromatography. The most abundant polyunsaturated and saturated fatty acids in normozoospermic samples were docosahexaenoic acid (DHA 22 : 6 ,3, 98.5 ± 4.5 nmol per 108 spermatozoa, mean ± SE) and palmitic acid (103 ± 17 nmol per 108 spermatozoa) respectively. The mean ± SE values of DHA and palmitic acid in asthenozoospermic samples were 53.9 ± 11.6 and 145 ± 14.7 nmol per 108 spermatozoa respectively. Compared with normozoospermic samples, asthenozoospermic samples showed lower levels of PUFA and higher amount of saturated fatty acids. The mean ± SE ratios of sperm PUFA/saturated fatty acids in asthenozoospermic and normozoospermic samples were 0.66 ± 0.06 and 1.45 ± 0.16 (P < 0.001) respectively. This study demonstrates that spermatozoa of asthenozoospermic men have lower levels of PUFA compared with saturated fatty acids. This may be contributory to the poor motility noted in samples from these men. [source] Feeding of different levels of metabolite combinations produced by Lactobacillus plantarum on growth performance, fecal microflora, volatile fatty acids and villi height in broilersANIMAL SCIENCE JOURNAL, Issue 2 2010Teck C. LOH ABSTRACT The effects of feeding different dosages of metabolite combination of L. plantarum RS5, RI11, RG14 and RG11 strains (Com3456) on the performance of broiler chickens was studied. A total of 504 male Ross broilers were grouped into 7 treatments and offered different diets: (i) standard corn-soybean based diet (negative control); (ii) standard corn-soybean based diet +100 ppm neomycin and oxytetracycline (positive control); (iii) standard corn-soybean based diet + 0.1% metabolite combination of L. plantarum RS5, RI11, RG14 and RG11 strains (Com3456); (iv) standard corn-soybean based diet + 0.2% of Com3456; (v) standard corn-soybean based diet + 0.3% of Com3456 (vi) standard corn-soybean based diet + 0.4% of Com3456 and (vii) standard corn-soybean based diet + 0.5% of Com3456. Supplementation of Com3456 with different dosages improved growth performance, reduced Enterobacteriaceae and increased lactic acid bacteria count, and increased villi height of small intestine and fecal volatile fatty acid concentration. Treatment with 0.4% and 0.2% Com3456 had the best results, especially in terms of growth performance, feed conversion ratio and villi height among other dosages. However, the dosage of 0.2% was recommended due to its lower concentration yielding a similar effect as 0.4% supplementation. These results indicate that 0.2% is an optimum level to be included in the diets of broiler in order to replace antibiotic growth promoters. [source] Effects of ruminal dosing of mechanical stimulating brush on digestibility and rumen fermentation status in Holstein steers fed low rice straw at different lengthsANIMAL SCIENCE JOURNAL, Issue 1 2004Ken-ichi HORIGUCHI ABSTRACT The objectives of the present study were to determine the effect of ruminal dosing of mechanical stimulating brush (Rumen faibu; RF) on digestibility and on rumen fermentation status in Holstein steers fed high concentrate and low rice straw. Eight steers (461 kg in average bodyweight) were used. Four steers were orally dosed three RF per head (RF-dosing) and the other four were not dosed (non-RF). All steers were fed enough concentrate and rice straw to gain 1.4 kg/day in bodyweight. Rice straw was cut 2 cm and 30 cm. The organic cell wall content of feeding diets was 17% in dry matter basis. Digestibility and nutritive value were not affected by RF dosing and length of rice straw. Ruminal pH was significantly lower in RF-dosing group than in non-RF group after feeding, and tended to be higher in long rice straw than in short rice straw throughout the day. There were no clear difference on total volatile fatty acid concentration and molar proportion of volatile fatty acid by RF dosing and length of rice straw throughout examined period. These present results suggest that digestibility and rumen fermentation status are not affected by RF dosing when organic cell wall content of feeding diets is approximately 17% in dry matter with rice straw over 2 cm. [source] Clinical acidosis in a Gippsland dairy herdAUSTRALIAN VETERINARY JOURNAL, Issue 6 2005E. BRAMLEY Objective: To report on spontaneous clinical and subclinical acidosis in a large dairy herd, to evaluate the diets and feeding strategies involved, and to report on measures of rumen function in the cows affected. Design: A Gippsland dairy herd was sampled as part of a wider randomised cross-sectional study that examined the prevalence, risk factors for, and effects of, acidosis on rumen function of dairy cattle. Three herds on the farm were involved in the study: the transition herd (cows 3 weeks prior to calving), the very fresh lactating herd (1 Fatty acid composition in wild and cultivated pacu and pintado fishEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Issue 2 2009Augusto Tanamati Abstract The fatty acid compositions of muscle tissue taken from wild strains of pintado (Pseudoplatystoma corruscans) and pacu (Piaractus mesopotamicus) fish, which were taken from the Brazilian Pantanal, were compared to the fatty acid compositions of tissue taken from two corresponding cultivated strains, which were fed commercial diets. The cultivated species possessed lipid contents of 12.2% (pacu) and 8.9% (pintado) while the wild species contained 7.9% (pacu) and 2.5% (pintado) lipids. Despite the high lipid contents of the cultivated pintado and pacu, the n -3 polyunsaturated fatty acid concentrations in muscle tissue were higher in wild pintado (224.9,mg/g flesh) and wild pacu (485.1,mg/g flesh) than in their respective cultivated strains (129.8 and 106.1,mg/g flesh, respectively). The n -6/n -3 ratios of pacu were 1.2 (wild) and 9.8 (cultivated), and those of pintado were 1.0 (wild) and 7.3 (cultivated). The fatty acid composition of pacu and pintado are strongly influenced by habitat and diet. [source] The concentrations of fatty acids in organo-mineral particle-size fractions of a ChernozemEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 3 2004G. Jandl Summary Fatty acids, the most abundant class of soil lipids, indicate pedogenetic processes and soil management. However, their quantitative distribution in organo-mineral particle-size fractions is unknown. The concentrations of n -C10:0 to n -C34:0 fatty acids both in whole soil samples and in the organo-mineral particle-size fractions of the Ap horizon of a Chernozem were determined (i) to evaluate the effects of long-term fertilization and (ii) to investigate their influence on the aggregation of organo-mineral primary particles. Quantification by gas chromatography/mass spectrometry (GC/MS) showed that long-term fertilization with nitrogen, phosphorus and potassium (NPK) and farmyard manure (FYM) led to larger concentrations (25.8 µg g,1) of fatty acids than in the unfertilized sample (22.0 µg g,1). For particle-size fractions of the unfertilized soil, the fatty acid concentrations increased from the coarse silt to the clay fractions (except for fine silt). Fertilization with NPK and FYM resulted in absolute enrichments of n -C21:0 to n -C34:0 fatty acids with a maximum at n -C28:0 in clay (×2.2), medium silt (×2.0), coarse silt (×1.8) and sand (×2.9) compared with the unfertilized treatment (the factors of enrichment are given in parentheses). New evidence for the aggregate stabilizing function of n -C21:0 to n -C34:0 fatty acids was shown by the characteristic pattern in size-fractionated, disaggregated and aggregated samples. Highly significant correlations of fatty acid concentrations with organic C concentrations and specific surface areas are interpreted as indicators of (i) trapping of fatty acids in organic matter macromolecules and (ii) direct bonding to mineral surfaces. This interpretation was supported by the thermal volatilization and determination of fatty acids by pyrolysis-field ionization mass spectrometry (Py-FIMS). [source] Studies on feed digestibilities in captive Asian elephants (Elephas maximus)JOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2003M. Clauss Summary In order to test the suitability of the horse as a nutritional model for elephants, digestibility studies were performed with six captive Asian elephants on six different dietary regimes, using the double marker method with acid detergent lignin as an internal and chromium oxide as an external digestibility marker. Elephants resembled horses in the way dietary supplements and dietary crude fibre content influenced digestibility, in calcium absorption parameters and in faecal volatile fatty acid composition. However, the absolute digestibility coefficients achieved for all nutrients are distinctively lower in elephants. This is because of much faster ingesta passage rates reported for elephants. No answer is given to why elephants do not make use of their high digestive potential theoretically provided by their immense body weight. Differences in volatile fatty acid concentrations between these captive elephants and those reported from elephants from the wild are in accord with a reported high dependence of free-ranging elephants on browse forage. Zusammenfassung Untersuchungen zur Verdaulichkeit von Futtermitteln bei Asiatischen Elefanten (Elephas maximus) Um zu überprüfen, ob das Pferd als ernährungsphysiolgisches Modelltier für Elefanten herangezogen werden kann, wurden Verdaulichkeitsstudien an sechs im Zoo gehaltenen Indischen Elefanten mit sechs verschiedenen Futterrationen durchgeführt. Dabei wurde die Doppelindikatormethode mit Lignin als internem und Chromoxid als externem Marker verwendet. Elefanten ähnelten Pferden hinsichtlich der Art und Weise, wie sich Ergänzungsfuttermittel und der Rohfasergehalt der Ration auf die Verdaulichkeit auswirkten, hinsichtlich der Kalziumabsorption, und hinsichtlich der Zusammensetzung der flüchtigen Fettsäuren im Kot. Die von Elefanten erzielten absoluten Verdaulich-keitswerte sind jedoch für alle Nährstoffe deutlich niedriger als bei Pferden, was auf die schnellere Passage der Ingesta durch den Verdauungstrakt der Elefanten zurückzuführen ist. Die Frage, warum Elefanten ihr großes Potential zu hohen Verdauungleistungen nicht ausnutzen, das ihnen theoretisch aufgrund ihrer immensen Körpermasse gegeben ist, ist noch nicht beantwortet. Unterschiede in den Konzentrationen von flüchtigen Fettsäuren im Kot zwischen diesen Zoo-Elefanten und Elefanten aus freier Wildbahn spiegeln den hohen Laubanteil in der Nahrung freilebender Tiere wieder. [source] SEASONAL VARIATIONS IN FATTY ACID COMPOSITION OF OIL IN DEVELOPING COCONUTJOURNAL OF FOOD QUALITY, Issue 2 2009S. NARESH KUMAR ABSTRACT Studies on seasonal variation in oil and fatty acid profile of developing solid endosperm of two cultivars, West Coast Tall (WCT) and Chowghat Orange Dwarf (COD), and their hybrids indicated that oil percentage increased from 30% in 6-month-old nuts to 63% in matured nuts (12 months old). Nuts sampled during July from different levels of maturity had high oil percentage and followed by those sampled during April, October and January. During nut development to maturity, the percentages and contents of medium and long chain saturated fatty acids increased except that of palmitic and myristic acids. Concentration of long chain unsaturated fatty acids (LCUFAs) in developing coconut kernel were high at 5 and 6 months after fertilization and then decreased toward maturity. The LCUFAs were high in nuts developing during October; consequently, saturated to unsaturated fatty acid ratios were low during October. Results indicated that nuts matured during October had better nutritional quality for human consumption and those matured during January are more suitable for industrial purpose due to higher medium chain fatty acid concentrations. PRACTICAL APPLICATIONS Coconut is consumed either as the tender nut (5,6 months after fertilization) or as the kernel from mature nut (12 months after fertilization). Recent technologies of making snowball tender nut use the nuts aged 7,8 months old. Kernel also is consumed in this product. Apart from this, the coconut is being increasingly used for making different kernel-based value-added products. This information is useful, as the value-added products are being developed using different maturities of coconut. Hence, it is of paramount importance that the fatty acid profile of coconut kernel is known in detail for assessing the safety of food consumption from the human health point of view. Apart from this, information on the seasonal variation in fatty acid profile of developing endosperm gives an integrated knowledge so as to optimize the usage of coconut kernel for both human consumption and industrial exploitation. [source] Dose-Titration Effects of Fish Oil in Osteoarthritic DogsJOURNAL OF VETERINARY INTERNAL MEDICINE, Issue 5 2010D. Fritsch Background: Food supplemented with fish oil improves clinical signs and weight bearing in dogs with osteoarthritis (OA). Objective: Determine whether increasing the amount of fish oil in food provides additional symptomatic improvements in OA. Animals: One hundred and seventy-seven client-owned dogs with stable chronic OA of the hip or stifle. Methods: Prospective, randomized clinical trial using pet dogs. Dogs were randomly assigned to receive the baseline therapeutic food (0.8% eicosopentanoic acid [EPA] + docosahexaenoic acid [DHA]) or experimental foods containing approximately 2- and 3-fold higher EPA+DHA concentrations. Both veterinarians and owners were blinded as to which food the dog received. On days 0, 21, 45, and 90, serum fatty acid concentrations were measured and veterinarians assessed the severity of 5 clinical signs of OA. At the end of the study (day 90), veterinarians scored overall arthritic condition and progression of arthritis based on their clinical signs and an owner interview. Results: Serum concentrations of EPA and DHA rose in parallel with food concentrations. For 2 of 5 clinical signs (lameness and weight bearing) and for overall arthritic condition and progression of arthritis, there was a significant improvement between the baseline and 3X EPA+DHA foods (P=.04, .03, .001, .0008, respectively) but not between the baseline and the 2X EPA+DHA foods. Conclusions and Clinical Importance: Increasing the amount of fish oil beyond that in the baseline food results in dose-dependent increases in serum EPA and DHA concentrations and modest improvements in the clinical signs of OA in pet dogs. [source] Randomized placebo-controlled trial assessing the effect of bifidobacteria-fermented milk on active ulcerative colitisALIMENTARY PHARMACOLOGY & THERAPEUTICS, Issue 10 2004K. Kato Summary Background :,Probiotics are efficacious for treating and maintaining remission of ulcerative colitis. Aim :,To conduct a randomized placebo-controlled trial of bifidobacteria-fermented milk supplementation as a dietary adjunct in treating active ulcerative colitis. Methods :,Twenty patients with mild to moderate, active, ulcerative colitis randomly received 100 mL/day of bifidobacteria-fermented milk or placebo for 12 weeks with conventional treatment. Results :,Clinical and endoscopic activity indices and histological scores were similar in the two groups before treatment. Although improvements were significant in both groups, the clinical activity index was significantly lower in the bifidobacteria-fermented milk than in the placebo group after treatment. The post-treatment endoscopic activity index and histological score were significantly reduced in the bifidobacteria-fermented milk, but not the placebo group. Increases in faecal butyrate, propionate and short-chain fatty acid concentrations were significant in the bifidobacteria-fermented milk, but not the placebo group. No adverse effects were observed in either group. Conclusion :,Supplementation with this bifidobacteria-fermented milk product is safe and more effective than conventional treatment alone, suggesting possible beneficial effects in managing active ulcerative colitis. This is a pilot study and further larger studies are required to confirm the result these preliminary results. [source] Effect of fat supplementation during transition period on plasma leptin and non-esterified fatty acid concentrations in Holstein cowsANIMAL SCIENCE JOURNAL, Issue 3 2010Ahmad AFZALZADEH ABSTRACT The objective of this experiment was to investigate the effect of fat supplementation during the transition period on pre and postpartum body weight (BW), body condition score (BCS), non-esterified fatty acids (NEFA), glucose and leptin concentrations in Holstein cows. Holstein cows (n = 15) received a low fat diet (LF; 1.61 Mcal net energy for lactation (NEL)/kg of dry matter [DM]), moderate fat diet (MF; 1.68 Mcal NEL/kg DM) or a high fat diet (HF; 1.74 Mcal NEL/kg DM) for 4 weeks prior to calving. All cows were fed similar lactation diets ad libitum (1.74 Mcal NEL/kg DM) for 30 days after calving. Increasing diet energy density during transition period had no effect on prepartum DMI, BCS, BW, glucose and NEFA concentrations (P > 0.05); but leptin concentrations and energy balance (EB) were affected by treatments (P < 0.05). Animals fed HF had less plasma leptin prepartum. After parturition, BW, milk production, milk fat, protein, urea nitrogen and plasma glucose concentrations were affected by prepartum diets (P < 0.05). Fat supplementation prepartum did not affect postpartum NEFA. In conclusion, prepartum fat supplementation decreased leptin concentration prepartum. [source] Effects of starch and protein sources on starch disappearance in the gastrointestinal tract of Hanwoo (Korean native) steersANIMAL SCIENCE JOURNAL, Issue 3 2010Kyoung Hoon KIM ABSTRACT Hanwoo (Korean native) steers (274.8 ± 4.6 kg) with ruminal and duodenal cannulae were used in a 4 × 4 Latin square design experiment to examine the effects of dietary treatments on starch disappearance in the gastrointestinal tract. Dietary treatments consisted of concentrate that were based on ground corn with soybean meal (C-SBM), ground corn with corn gluten meal (C-CGM), ground barley with soybean meal (B-SBM) and ground barley with corn gluten meal (B-CGM). Although the intakes of starch and protein for steers fed experimental diets were different, it did not change ruminal pH and total volatile fatty acid concentrations. Average duodenal CP flow and quantity of CP apparently digested post-ruminally was higher (P = 0.001) for CGM-based diets than SBM-based diets. There were increases in quantity (P < 0.001) and percentage (P < 0.001) of corn starch digested post-ruminally compared to barley starch. Synchronized diets showed higher percentages (P = 0.03) of starch apparently digested post-ruminally than asynchronization. Hanwoo steers fed a corn-based diet with a large quantity of starch reaching the duodenum and fed C-CGM supplying great amounts of protein to the small intestine may have contributed to increased post-ruminal starch digestion. [source] Novel application of oxygen-transferring membranes to improve anaerobic wastewater treatmentBIOTECHNOLOGY & BIOENGINEERING, Issue 4 2005Anthony S. Kappell Abstract Anaerobic biological wastewater treatment has numerous advantages over conventional aerobic processes; anaerobic biotechnologies, however, still have a reputation for low-quality effluents and operational instabilities. In this study, anaerobic bioreactors were augmented with an oxygen-transferring membrane to improve treatment performance. Two anaerobic bioreactors were fed a synthetic high-strength wastewater (chemical oxygen demand, or COD, of 11,000 mg l,1) and concurrently operated until biomass concentrations and effluent quality stabilized. Membrane aeration was then initiated in one of these bioreactors, leading to substantially improved COD removal efficiency (>95%) compared to the unaerated control bioreactor (,65%). The membrane-augmented anaerobic bioreactor required substantially less base addition to maintain circumneutral pH and exhibited 75% lower volatile fatty acid concentrations compared to the unaerated control bioreactor. The membrane-aerated bioreactor, however, failed to improve nitrogenous removal efficiency and produced 80% less biogas than the control bioreactor. A third membrane-augmented anaerobic bioreactor was operated to investigate the impact of start-up procedure on nitrogenous pollutant removal. In this bioreactor, excellent COD (>90%) and nitrogenous (>95%) pollutant removal efficiencies were observed at an intermediate COD concentration (5,500 mg l,1). Once the organic content of the influent wastewater was increased to full strength (COD = 11,000 mg l,1), however, nitrogenous pollutant removal stopped. This research demonstrates that partial aeration of anaerobic bioreactors using oxygen-transferring membranes is a novel approach to improve treatment performance. Additional research, however, is needed to optimize membrane surface area versus the organic loading rate to achieve the desired effluent quality. © 2005 Wiley Periodicals, Inc. [source] EPA supplementation improves teacher-rated behaviour and oppositional symptoms in children with ADHDACTA PAEDIATRICA, Issue 10 2010Per A Gustafsson Abstract Aim:, Measure efficacy of eicosapentaenoic acid (EPA) in children with attention deficit hyperactivity disorder (ADHD). Methods:, Randomized controlled trial (RCT) of 0.5 g EPA or placebo (15 weeks) in 92 children (7,12 years) with ADHD. Efficacy measure was Conners' Parent/Teacher Rating Scales (CPRS/CTRS). Fatty acids were analysed in serum phospholipids and red blood cell membranes (RBC) at baseline and endpoint with gas chromatography. Results:, EPA improved CTRS inattention/cognitive subscale (p = 0.04), but not Conners' total score. In oppositional children (n = 48), CTRS total score improved ,25% in 48% of the children receiving EPA vs. 9% for placebo [effect size (ES) 0.63, p = 0.01]. In less hyperactive/impulsive children (n = 44), ,25% improvement was seen in 36% vs. 18% (ES 0.41, n.s.), and with both these types of symptoms 8/13 with EPA vs. 1/9 for placebo improved ,25% (p = 0.03). Children responding to treatment had lower EPA concentrations (p = 0.02), higher AA/EPA (p = 0.005) and higher AA/DHA ratios (p = 0.03) in serum at baseline. Similarly, AA/EPA (p = 0.01), AA/DHA (p = 0.038) and total omega-6/omega-3 ratios (p = 0.028) were higher in RBC, probably because of higher AA (p = 0.011). Conclusion:, Two ADHD subgroups (oppositional and less hyperactive/impulsive children) improved after 15-week EPA treatment. Increasing EPA and decreasing omega-6 fatty acid concentrations in phospholipids were related to clinical improvement. [source] Mutual influence of protein and lipid feed content on European catfish (Silurus glanis) growthJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2 2004E. Has-Schön Summary We wished to determine protein and lipid content in pelleted raw fish food, necessary for optimal growth of European catfish (Silurus glanis). Experiments were set up in 20 cages, each holding 30 young catfish. Fishes in each cage received a different food combination over a 98-day period at favourable physical and chemical water conditions. Food protein content varied between 37.5 and 45%, while lipid content, added in the form of soybean oil, varied between 3 and 11%. The oil contained an adequate , -fatty acids concentration, necessary for fish growth. The main growth indicators determined at the end of experiment were total body gain, specific growth rate and feed conversion ratio. There was a high statistical difference among the experimental groups receiving variable food combinations for each growth parameter (P < 0.001). Both protein and lipid food content affected growth parameters, but in a different manner. Further analysis , percentage of change depending on lipid to protein ratio and bivariate surface analysis , allowed us to recognize the most economical combination: 39.5% protein + 9% lipid content. The addition of 9% soybean oil to the fish food reduces the necessary protein concentration by 5.5%, with resulting identical catfish growth effects. [source] Antioxidant capacity of human milk and its association with vitamins A and E and fatty acid compositionACTA PAEDIATRICA, Issue 11 2009A Tijerina-Sáenz Abstract Aim:, The antioxidant capacity of human milk reflects the presence and activity of multiple components, which prevent oxidative rancidity. The aim of this study was to use the Oxygen Radical Absorbance Capacity assay to assess human milk antioxidant capacity and find correlations with milk components. Methods:, Milk samples collected from 60 breastfeeding women at 1 month postpartum were assayed for antioxidant capacity, vitamins E and A, and fatty acids. Potential statistical relationships of concentrations of vitamins A and E and polyunsaturated fatty acids on the antioxidant capacity of human milk were determined. Results:, Human milk antioxidant capacity was positively attributed to ,-tocopherol concentration (, < 0.05). The vitamin A concentration did not significantly contribute to milk antioxidant capacity, but was correlated to milk ,-tocopherol concentration (r = 0.587; , < 0.001). There was no evidence of an inverse relationship between polyunsaturated fatty acids concentration and the antioxidant capacity value of milk. Conclusion:, This study shows that ,-tocopherol is an important contributor to the oxidative stability of human milk. Moreover, there was no evidence obtained to show that women who have high levels of milk polyunsaturated fatty acids are predisposed to lower milk antioxidant capacity. [source] Are Hormonal Responses to Exercise in Young Men with Down's Syndrome Related to Reduced Endurance Performance?JOURNAL OF NEUROENDOCRINOLOGY, Issue 5 2008V-A. Bricout The aim of the present study was to analyse whether hormonal responses could explain an exercise limitation in Down's syndrome (DS). Fourteen young men with DS (mean age 22.5 ± 0.7 years) and 15 controls (CONT, mean age 22.5 ± 0.3 years) participated in the study. During a treadmill submaximal incremental test, blood samples were collected for determination of hormonal and metabolic variables. Compared to CONT, DS individuals showed lower VO2max (P < 0.05), and lower duration of submaximal incremental exercise (P < 0.001). At rest, DS individuals showed greater catecholamines, insulin and leptin values (P < 0.05), but lower testosteronemia and cortisolemia (P < 0.05), compared to CONT. During submaximal incremental tests, catecholamines and cortisol were not increased, whereas the insulin concentration of DS individuals was significantly higher (P < 0.01) compared to CONT. Glycaemia increased significantly at the end of submaximal incremental test for CONT but not for DS individuals (P < 0.01). Maximal fat oxidation was lower (P < 0.01), whereas non-esterified fatty acids concentrations rose significantly during submaximal exercise in DS individuals. These results indicate an altered hormonal response to exercise in DS individuals. This endocrine profile at rest and during exercise may limit endurance performance in DS individuals. [source] Influence of replacing brewers' grains with green tea grounds on feed intake, digestibility and ruminal fermentation characteristics of wethersANIMAL SCIENCE JOURNAL, Issue 2 2008Chuncheng XU ABSTRACT An experiment was conducted to examine feed intake, apparent digestibility, nitrogen balance, ruminal fermentation and blood components of wethers fed diets containing increasing levels of wet green tea grounds (WGTG). The experimental design was a 4 × 4 Latin square with four wethers in four 15-day periods. Wethers were allowed access to diets ad libitum, and allotted to one of four treatments in which WGTG replaced 0% (no WGTG added, CTG), 5% (low level, LBG), 10% (medium level, MTG), and 15% (high level, HTG) of total mixed ration (TMR) dry matter (DM) as wet brewers grains (WBG). All TMR silages were ensiled for 120 days and, irrespective of the WGTG addition, they were well preserved with a high lactic acid content, low pH and ammonia-N contents. There were no differences among treatments in feed intake, with the exception of ether extract intake (P = 0.032). Digestibilities for LTG and MTG treatments were not different from CTG. However, the organic matter, crude protein, acid detergent fiber and energy digestibilities for HTG treatment were lower than the CTG (P < 0.05). As the level of WGTG increased, nitrogen intake did not differ, but fecal nitrogen increased (P = 0.002), while urinary nitrogen decreased (P < 0.001). No differences among treatments were found in pH level and volatile fatty acids concentrations. However, the ruminal ammonia-N of the HTG silage was lower than for the CTG silage at all times (P < 0.05). Increasing concentrations of WGTG in the TMR silage decreased (P = 0.019) plasma urea nitrogen content. Therefore, the possible mixing proportion of WGTG for TMR silages can be 10% of the diet DM. [source]
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