			Home About us Contact

Factor Isolated (factor + isolated)

Distribution by Scientific Domains

Chemistry	40%

Selected Abstracts

Identification of a potent antibacterial factor isolated from bronchoalveolar lavage fluid: guanidine, N -[3-[(aminoiminomethyl)amino]propyl]- N -dodecyl-, a potential source of error in the analysis of antibacterial agents

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 3 2003
Mirna Abraham-Nordling
The widespread use of antibiotics in modern society has encouraged the search for new antibacterial compounds. In this laboratory investigations are being made to identify and characterise novel antibacterial peptides. With this in mind, the antibacterial properties of human bronchoalveolar lavage (BAL) fluid from sarcoidosis patients is being investigated. In this communication we report on the identification and characterisation of a highly active non-peptide antibacterial compound isolated from BAL fluid. The structure of this active compound was elucidated by high-resolution accurate mass and tandem mass spectrometry to be guanidine, N -[3-[(aminoiminomethyl)amino]propyl]- N -dodecyl-. This compound does not appear to be endogenous, and its presence in BAL fluid extracts presents a potential source of error in analysis of antibacterial agents. The biological effects of guanidine, N -[3-[(aminoiminomethyl)amino]propyl]- N -dodecyl- have not previously been described in the literature. Copyright © 2002 John Wiley & Sons, Ltd. [source]

In vitro activity of a cytotoxic factor isolated from human leukocytes,

APMIS, Issue 6 2006
CARL FREDRIK NAESS-ANDRESEN
The aim of the present study was to test the in vitro antimicrobial properties of a cytotoxic factor isolated from human leukocytes and for an effect on DNA synthesis. A microdilution broth method was used to determine MIC values for fungi and bacteria. Flow cytometry was used to examine the effect on DNA synthesis. The MIC values for fungi were in the range 1,128 ,g CF/mL and concentrations 2,4 times the MIC were fungicidal. CF had no effect on bacteria (MIC and MBC values > 1024 ,g CF/mL). Growth experiments performed with increasing concentration of CF showed a dose-dependent effect on growth of the yeasts tested. We confirmed that zinc ions could abrogate the antiproliferative effect of CF. Flow cytometric experiments showed that CF had no effect on the cell cyclus. The present study shows that this endogenous substance isolated from human leukocytes has growth inhibitory properties towards fungi. Because of the origin from human leukocytes, CF may have a role in the non-specific defense against fungal infections. The use of CF may potentially have a role in the treatment of infections caused by fungi. Further investigations to characterize CF chemically and to study the antifungal mechanism are necessary. [source]

Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation

FEBS JOURNAL, Issue 16 2010
Yuki Moritani
Proteoliposomes were directly prepared by synthesizing membrane proteins with the use of minimal protein synthesis factors isolated from Escherichia coli (the PURE system) in the presence of liposomes. Connexin-43 (Cx43), which is a water-insoluble integral membrane protein that forms a hexameric complex in membranes, was cotranslationally integrated with an essentially uniform orientation in liposomes. The addition of liposomes following protein expression (post-translational presence of liposomes) did not lead to the integration of Cx43 into the liposome membranes. The amount of integrated Cx43 increased as the liposome concentration increased. The presence of liposomes did not influence the total amount of synthesized Cx43. The Cx43 integrated into the liposome membranes formed open membrane pores. These results indicate that the liposomes act in a chaperone-like manner by preventing Cx43 from aggregating in solution, because of integration into the bilayer, and also by functionalization of the integrated Cx43 in the membrane. This is the first report that cell-free-synthesized water-insoluble membrane protein is directly integrated with a uniform orientation as a functional oligomer into liposome membranes. This simple proteoliposome preparation procedure should be a valuable approach for structural and functional studies of membrane proteins. Structured digital abstract ,,MINT-7900670: Cx-43 (uniprotkb:P08050) and Cx-43 (uniprotkb:P08050) bind (MI:0407) by cross-linking study (MI:0030) [source]

Does corporate governance transparency affect the accuracy of analyst forecasts?

ACCOUNTING & FINANCE, Issue 5 2006
Gauri Bhat
M4; O1 Abstract Using country-level proxies for corporate governance transparency, this paper investigates how differences in transparency across 21 countries affect the average forecast accuracy of analysts for the country's firms. The association between financial transparency and analyst forecast accuracy has been well documented in previous published literature; however, the association between governance transparency and analyst forecast accuracy remains unexplored. Using the two distinct country-level factors isolated by Bushman et al. (2004), governance transparency and financial transparency, we investigate whether corporate governance information impacts on the accuracy of earnings forecasts over and above financial information. We document that governance transparency is positively associated with analyst forecast accuracy after controlling for financial transparency and other variables. Furthermore, our results suggest that governance-related disclosure plays a bigger role in improving the information environment when financial disclosures are less transparent. Our empirical evidence also suggests that the significance of governance transparency on analyst forecast accuracy is higher when legal enforcement is weak. [source]