Home About us Contact
|
Home About us Contact | ||
|
|
||
FSH Administration (fsh + administration)
Selected AbstractsIn-vitro Fertilization and Embryo Transfer and Cellular Immunity: Study on Cytokines and T Lymphocyte subpopulations in IVF-ET CyclesJOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (ELECTRONIC), Issue 3 2002Mitsutaka Murakami Objectives: To determine whether peripheral T lymphocyte subpopulations and cytokines change during in-vitro fertilization and embryo transfer (IVF-ET) cycles and to evaluate them with regard to pregnancy status and types of infertility. Methods: Peripheral T lymphocyte subpopulations and cytokines in 33 consecutive cycles of IVF-ET were examined. All the women were stimulated with purified FSH and hCG after pituitary suppression with GnRH agonist. Peripheral blood samples were collected before FSH administration, on the day of hCG administration, the day of ET (day 2), day 6 and day 15. We measured plasma estradiol and progesterone levels and plasma interferon-,, interleukin-4 (IL-4), IL-10 and IL-12 levels. Peripheral T lymphocyte subpopulations, T helper type 1 and 2 cells (Th1 and Th2) and T cytotoxic type 1 and 2 cells (Tc1 and Tc2), were analyzed with three-color flowcytometry. Results: There were no changes in the plasma levels of the cytokines or in the proportions of Th1 and Th2 and the proportions of Tc1 and Tc2 in peripheral blood lymphocytes during the IVF-ET cycles. In women with endometriosis, the ratios of Tc1 to CD8+ lymphocytes and the Tc1 to Tc2 ratios before FSH administration were much higher than in women without endometriosis. The ratios of Tc1 to CD8+ lymphocytes were significantly lower in the patients with endometriosis who became pregnant. Conclusions: Peripheral cellular immunity does not change during IVF-ET cycles. In women with endometriosis, the peripheral Tc1 subpopulation is more predominant before ovarian stimulation, suggest- ing that the ratio of Tc1 before ovarian stimulation could be an indicator of fecundity for women with endometriosis. [source] Ovum Pick-up in Cycling and Lactating Postpartum Swamp Buffaloes (Bubalis bubalis)REPRODUCTION IN DOMESTIC ANIMALS, Issue 2 2005A Promdireg Contents The objective of this study was to evaluate the efficiency of Ovum Pick Up (OPU) in cycling (n = 5) and lactating, postpartum, swamp buffaloes (n = 6) with and without gonadotropin stimulation. The OPU was performed every two weeks in all groups of animals, for a total of six sessions. Thirty collections were performed in five cycling buffaloes and 36 collections in six lactating postpartum buffaloes. Buffaloes that received hormonal stimulation were given a total of 400 mg, follicle stimulating hormone (FSH), administered twice daily over 3 days in decreasing doses, together with 100 ,g of GnRH, 24 h after the last FSH injection. Following a resting period of 1 month, the two groups of buffaloes, were subjected to the same OPU regimen, but without any hormonal treatment for an additional six OPU sessions. The number of aspirated follicles recorded from the hormonal stimulated, cycling animals and lactating, postpartum buffaloes was not significantly different, 7.2 ± 3.7 and 9.0 ± 3.2, respectively (p > 0.05). Recovered oocytes collected from the two groups of hormonally stimulated animals were also not statistically different: 3.7 ± 2.7 in the cycling and 5.9 ± 3.5 in the lactating postpartum group (p > 0.05). In the two groups of buffaloes not receiving hormonal stimulation, the number of aspirated follicles was not significantly different: 2.1 ± 1.4 and 1.4 ± 0.7 in cycling and lactating postpartum buffaloes respectively (p > 0.05). Recovered oocytes in the non-treated groups were also similar: 1.4 ± 1.3 vs 0.7 ± 0.8 in cycling and lactating buffaloes (p > 0.05). Among stimulated buffaloes, most aspirated follicles were small in size (,5 mm), whereas they were mostly medium and large sizes in the non-treated buffaloes. The oocyte recovery rate in both the groups, cycling and lactating postpartum, were 51.6% and 69.5% in stimulated groups and 55.0% and 53.1% in non-stimulated groups (p > 0.05). The majority of recovered oocytes were single- and multi-layered, and the number was greater in the cycling than in the lactating, postpartum buffaloes. The number and quality of recovered oocytes was similar in all groups of buffaloes whether they were received or did not receive hormonal stimulation. Moreover no difference was found in multi- and single-layered oocytes between cycling and lactating, postpartum buffaloes. In conclusion, OPU can be performed successfully in swamp buffalo in different reproductive status and FSH administration was shown to increase the number of aspirated oocytes in both cycling and lactating, postpartum buffaloes. [source] Endocrine Profiles and Embryo Quality in Japanese Black Cattle Superovulated with Human Menopausal Gonadotrophin and Porcine Follicle Stimulating HormoneREPRODUCTION IN DOMESTIC ANIMALS, Issue 2 2001M Sugano Induction of superovulation using human menopausal gonadotriphin (hMG) in Japanese Black cattle can result in the recovery of a higher percentage of high quality embryos compared with that using porcine follicle stimulating hormone (FSH). In order to clarify the endocrinological mechanism involved in this discrepancy, 30 superovulation sessions of 17 Japanese Black cattle were studied. Fifteen cattle were super-stimulated with hMG (total 600 IU), and the remaining 15 cattle were given FSH (total 20 mg). The plasma profiles of LH, estradiol-17, (E2) and progesterone (P4) were correlated, and the embryo quality was investigated. The total number of ova recovered and the number of transferable embryos tended to be larger in the hMG-treated group than in the FSH-treated group. The percentage of excellent embryos tended to be higher in the hMG-treated group than in the FSH-treated group (54.3 and 28.7%, respectively, p < 0.10). The E2 level increased during the first 3 days after the initial administration of either hMG or FSH and was higher in the hMG-treated group than in the FSH-treated group (p < 0.05). During this period, the E2 level could be categorized into one of the following three types according to whether it increased or decreased and according to the degree of increase or decrease: (1) increase by a factor of 1.2 or more (quick increase type) (2) slight increase by a factor less than 1.2 (slow increase type), and (3) no increase (unstable increase type). In the group treated with hMG, 66.7% of the animals (10 of 15 cattle) showed a quick increase in the E2 level. However, in the FSH-treated group, 40% (six of 15) of the animals showed a slow increase in the E2 level. The plasma LH level increased dramatically 8 h prior to the peak level in both the hMG- and FSH-treated groups, and then it returned to the basal level 12 h later. After the administration of prostaglandin (PG)F2,, the LH peak level was attained within 44 h in 80% of the animals in the hMG-treated group, whereas in the FSH-treated group, the LH peak level tended to be reached later. The P4 level did not increase during the period of hMG or FSH treatment and decreased drastically following administration of PGF2,. After the onset of oestrus, the P4 level was higher in the hMG group than in the FSH group, and 5 to 7 days after oestrus, the level remained higher in the hMG group than in the FSH group (p < 0.05). After the first 3 days of hMG administration, the E2/P4 ratio was higher than that after FSH administration. Furthermore, on the day following PGF2, administration, the ratio was significantly higher in the hMG group than in the FSH group (p < 0.05). These results indicate that superovulation in cattle given hMG results in a significant increase in plasma E2 during the first 3 days and that the increase in the plasma P4 level is larger a few days after oestrus and thereafter compared with FSH-induced superovulation. Therefore, such plasma level profiles may be related to the increased recovery rate of high quality embryos. [source] FSH and ovarian response: spontaneous recovery of pituitary,ovarian activity during the pill-free period vs. exogenous recombinant FSH during high-dose combined oral contraceptivesCLINICAL ENDOCRINOLOGY, Issue 4 2002A. M. Van Heusden Summary ojbective Compare spontaneous recovery of pituitary,ovarian activity during the pill-free period following the correct use of low-dose oral contraceptives and subsequent ovarian function during the administration of exogenous recombinant FSH (recFSH) after switching to continued Lyndiol® (2·5 mg lynestrenol + 0·05 mg ethinyl-oestradiol) medication. design Prospective, randomized, group-comparative, single-centre study. Following the monitoring of the pill-free period (week 1) and subsequent treatment with Lyndiol® (for a total of 5 weeks), all subjects were randomly allocated to one of four groups receiving daily FSH injections for 1 week [75, 150, 225 IU recFSH or 150 IU purified urinary FSH (uFSH)] during the fourth week of Lyndiol® use. patients Thirty-six healthy volunteers aged 18,39 years, prestudy oral contraceptive use for at least 3 months, cycle length between 24 and 35 days. measurements Serum FSH, LH and oestradiol (E2) concentrations as well as transvaginal ultrasound assessment of the number and diameter of follicles > 2 mm were used to monitor pituitary ovarian function. results At the start of the pill-free period following the prestudy contraceptive medication, 67% of the women presented with LH and FSH levels < 1 IU/l and only one follicle > 10 mm was observed. Initial levels of LH and FSH correlated (P < 0·05) with the extent of pituitary,ovarian activity during the pill-free period. At the end of the pill-free period a follicle > 10 mm had emerged in one subject only. During the first 3 days of Lyndiol® use, seven women (19%) eventually showed at least one follicle > 10 mm. During combined exogenous FSH and Lyndiol® administration, LH levels remained completely suppressed (, 0·5 IU/l) in all women studied. FSH levels and number and size of follicles increased with increasing doses of exogenous FSH in a dose-dependent manner. E2 levels remained low in all groups (< 150 pmol/l). During the week following FSH administration, FSH levels and E2 levels decreased gradually while the number of follicles > 10 mm still increased. conclusions We have confirmed that dominant follicles > 10 mm are present at the end of the pill-free period and during the first days after resumption of pill intake. Once follicles > 10 mm arose at the end of the pill-free period, continued use of Lyndiol® did not reduce follicle diameters. One week of Lyndiol® reduces pituitary,ovarian activity to levels observed after 3 weeks of low-dose pills. FSH administration during Lyndiol® resulted in dose-dependent follicle growth despite extremely low LH levels. E2 secretion (56 ± 51 pmol/l) occurred to a limited and variable extent along with extremely low serum LH concentrations. Recovery of pituitary,ovarian activity at the end of the pill-free period is comparable to FSH levels and follicle dynamics following 7 days of 75,150 IU/l recFSH. [source] | ||||||||||