Home About us Contact
|
Home About us Contact | ||
|
|
||
Extra
Kinds of Extra Terms modified by Extra Selected AbstractsToothache referred from auriculotemporal neuralgia: case reportINTERNATIONAL ENDODONTIC JOURNAL, Issue 9 2009R. A. Murayama Abstract Aim, To present a 52-year-old male patient who complained of intense pain of short duration in the region of the left external ear and in the ipsilateral maxillary second molar that was relieved by blockade of the auriculotemporal nerve in the infratemporal fossa. Summary, Extra- and intraoral physical examination revealed a trigger point that reproduced the symptoms upon finger pressure in the ipsilateral auriculotemporal nerve and in the outer auricular pavilion. The patient's medical history was unremarkable. The maxillary left second molar tooth was not responsive to pulp sensitivity testing and there was no pain upon percussion or palpation of the buccal sulcus. Periapical radiographs revealed a satisfactory root filling in the maxillary left second molar. On the basis of the clinical signs and symptoms, the auriculotemporal was blocked with 0.5 mL 2% lidocaine and 0.5 mL of a suspension containing dexamethasone acetate (8 mg mL,1) and dexamethasone disodium sulfate (2 mg mL,1), with full remission of pain 6 months later. The diagnosis was auriculotemporal neuralgia. Key learning point ,,Auriculotemporal neuralgia should be considered as a possible cause of nonodontogenic toothache and thus included in the differential diagnoses. ,,The blockade of the auriculotemporal nerve in the infratemporal fossa is diagnostic and therapeutic. It can be achieved with a solution of lidocaine and dexamethasone. [source] Early reattachment does not reverse atrophy and fat accumulation of the supraspinatus,an experimental study in rabbitsJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 3 2003Hans K. Uhthoff Abstract Introduction: Reattachment of the supraspinatus (SSP) tendon after spontaneous rupture leads to improved shoulder function. Whether this improvement of function is due to a reversal of muscle atrophy and fat accumulation known to occur after SSP rupture is still debated. Our previous study of late reattachment of SSP (12 weeks) failed to confirm a reversal of muscle atrophy and of fat accumulation. Purpose: To find out whether earlier reattachment (6 weeks) reverses atrophy and fat accumulation of the SSP. Material and methods: Reattachment group: in seven rabbits unilateral supraspinatus detachment, reattachment after 6 weeks and killing 6 weeks later. Detachment group: in seven rabbits unilateral supraspinatus detachment and killing 12 weeks later. The contralateral shoulders served as controls (n = 14). Determination of the supraspinatus constituents: muscle, extra- and intramuscular fat in volume and cross-sectional area. Results: Muscle tissue in the reattachment group (8.6 ml ± 1 s.d. = 0.6) and in the detachment group (8.9 ml ± 0.9) were less than in control supraspinati (10.2 ml ± 0.9, both p < 0.05). Extra- and intramuscular fat in the reattachment group (8.7% ± 3.2) was greater than in both, the detachment group (4.6% ± 3.5), and control supraspinati (2.8% ± 1.7, both p < 0.05). Conclusion: In the rabbit, reattachment of the SSP at 6 weeks did neither reverse muscle atrophy nor fat accumulation during the ensuing 6 weeks. However, earlier reattachment (6 weeks) when compared with later reattachment (12 weeks) prevented an increase in fat accumulation. On the other hand, the delay before reattaching the tendon did not lead to an increase in muscle atrophy. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved. [source] Patient decision aids in general practice: availability and usagePRESCRIBER, Issue 5 2009Article first published online: 16 MAR 200 This article is based on the MeReC Extra Using Patient Decision Aids. The article describes patient decision aids and considers their availability and use in practice. Copyright © 2009 Wiley Interface Ltd [source] Intra- and extrarenal arteries exhibit different profiles of contractile responses in high glucose conditionsBRITISH JOURNAL OF PHARMACOLOGY, Issue 8 2008K Nobe Background and purpose: The renal artery (RA) has been extensively investigated for the assessment of renal vascular function/dysfunction; however, few studies have focused on the intrarenal vasculature. Experimental approach: We devised a microvascular force measurement system, which allowed us to measure contractions of interlobar arteries (ILA), isolated from within the mouse kidney and prepared without endothelium. Key results: KCl (50 mM) induced similar force development in the aorta and RA but responses in the ILA were about 50% lower. Treatment of RA with 10 ,M phenylephrine (PE), 10 nM U46619 (thromboxane A2 analogue) or 10 ,M prostaglandin F2, elicited a response greater than 150% of that induced by KCl. In ILA, 10 nM U46619 elicited a response that was 130% of the KCl-induced response; however, other agonists induced levels similar to that induced by KCl. High glucose conditions (22.2 mM glucose) significantly enhanced responses in RA and ILA to PE or U46619 stimulation. This enhancement was suppressed by rottlerin, a calcium-independent PKC inhibitor, indicating that glucose-dependent, enhanced small vessel contractility in the kidney was linked to the activation of calcium-independent PKC. Conclusion and implications: Extra- and intrarenal arteries exhibit different profiles of agonist-induced contractions. In ILA, only U46619 enhanced small vessel contractility in the kidney, which might lead to renal dysfunction and nephropathy through reduced intrarenal blood flow rate. A model has been established, which will allow the assessment of contractile responses of intrarenal arteries from murine models of renal disease, including type 2 diabetes. British Journal of Pharmacology (2008) 155, 1204,1213; doi:10.1038/bjp.2008.365; published online 22 September 2008 [source] Approximal caries development in surfaces in contact with fluoride-releasing and non-fluoride-releasing restorative materials: an in situ studyEUROPEAN JOURNAL OF ORAL SCIENCES, Issue 6 2007Áine M. Lennon This study investigated the effect of compomer on initial interproximal caries development. One-hundred and sixty cylindrical, and 40 semispherical, bovine enamel samples (control) were prepared, polished, and sterilized. Sixty semicircular samples were prepared from each of the compomer Dyract eXtra and the fluoride-free composite Spectrum TPH. Samples were stored in water and fluoridated twice daily for 28 d. A baseline quantitative light fluorescence (QLF) image was made of each cylindrical sample. Twenty volunteers received intra-oral appliances with eight sample chambers. Each wing contained 1 control sample and either 3 Dyract eXtra or 3 Spectrum TPH samples in contact with the enamel surface of a cylindrical enamel sample. Appliances were worn for 24 h a day for 28 d except during toothbrushing (twice daily) and placement in 10% sucrose solution (five times daily). A final QLF image was made after 28 d. Caries development was analyzed as the lesion area × mean fluorescence loss (,Q % mm2) between these and the baseline images using QLF subtract software. The median ,Q was significantly lower in the Dyract eXtra group (,6.1% mm2) than in the Spectrum TPH (,13.9% mm2, P , 0.001) or control (,11.4% mm2, P = 0.03) groups. Teeth in contact with the compomer developed less caries compared with controls. [source] Gating of the expressed T-type Cav3.1 calcium channels is modulated by Ca2+ACTA PHYSIOLOGICA, Issue 4 2006L. Lacinová Abstract Aim:, We have investigated the influence of Ca2+ ions on the basic biophysical properties of T-type calcium channels. Methods:, The Cav3.1 calcium channel was transiently expressed in HEK 293 cells. Current was measured using the whole cell patch clamp technique. Ca2+ or Na+ ions were used as charge carriers. The intracellular Ca2+ was either decreased by the addition of 10 mm ethyleneglycoltetraacetic acid (EGTA) or increased by the addition of 200 ,m Ca2+ into the non-buffered intracellular solution. Various combinations of extra- and intracellular solutions yielded high, intermediate or low intracellular Ca2+ levels. Results:, The amplitude of the calcium current was independent of intracellular Ca2+ concentrations. High levels of intracellular Ca2+ accelerated significantly both the inactivation and the activation time constants of the current. The replacement of extracellular Ca2+ by Na+ as charge carrier did not affect the absolute value of the activation and inactivation time constants, but significantly enhanced the slope factor of the voltage dependence of the inactivation time constant. Slope factors of voltage dependencies of channel activation and inactivation were significantly enhanced. The recovery from inactivation was faster when Ca2+ was a charge carrier. The number of available channels saturated for membrane voltages more negative than ,100 mV for the Ca2+ current, but did not reach steady state even at ,150 mV for the Na+ current. Conclusions:, Ca2+ ions facilitate transitions of Cav3.1 channel from open into closed and inactivated states as well as backwards transition from inactivated into closed state, possibly by interacting with its voltage sensor. [source] Achieving high sexual size dimorphism in insects: females add instarsECOLOGICAL ENTOMOLOGY, Issue 3 2007TOOMAS ESPERK Abstract 1.,In arthropods, the evolution of sexual size dimorphism (SSD) may be constrained by a physiological limit on growth within each particular larval instar. A high SSD could, however, be attained if the larvae of the larger sex pass through a higher number of larval instars. 2.,Based on a survey of published case studies, the present review shows that sex-related difference in the number of instars is a widespread phenomenon among insects. In the great majority of species with a sexually dimorphic instar number, females develop through a higher number of instars than males. 3.,Female-biased sexual dimorphism in final sizes in species with sexually dimorphic instar number was found to considerably exceed a previously estimated median value of SSD for insects in general. This suggests a causal connection between high female-biased SSD, and additional instars in females. Adding an extra instar to larval development allows an insect to increase its adult size at the expense of prolonged larval development. 4.,As in the case of additional instars, SSD is fully formed late in ontogeny, larval growth schedules and imaginal sizes can be optimised independently. No conflict between selective pressures operating in juvenile and adult stages is therefore expected. 5.,In most species considered, the number of instars also varied within the sexes. Phenotypic plasticity in instar number may thus be a precondition for a sexual difference in instar number to evolve. [source] Parasites in the food web: linking amphibian malformations and aquatic eutrophicationECOLOGY LETTERS, Issue 7 2004Pieter T. J. Johnson Abstract Emerging diseases are an ever-growing affliction of both humans and wildlife. By exploring recent increases in amphibian malformations (e.g. extra or missing limbs), we illustrate the importance of food web theory and community ecology for understanding and controlling emerging infections. Evidence points to a native parasite, Ribeiroia ondatrae, as the primary culprit of these malformations, but reasons for the increase have remained conjectural. We suggest that the increase is a consequence of complex changes to aquatic food webs resulting from anthropogenic disturbance. Our results implicate cultural eutrophication as a driver of elevated parasitic infection: (1) eutrophication causes a predator-mediated shift in snail species composition toward Planorbella spp., (2) Planorbella are the exclusive first intermediate hosts of R. ondatrae and (3) Ribeiroia infection is a strong predictor of amphibian malformation levels. Our study illustrates how the effects of anthropogenic disturbance on epidemic disease can be mediated through direct and indirect changes in food web structure. [source] Symptomatic stenosis of the vertebrobasilar arteries: results of extra- and intracranial stent-PTAEUROPEAN JOURNAL OF NEUROLOGY, Issue 1 2009T. Seifert Background and purpose:, About half of all transient ischaemic attacks (TIAs) or strokes in the posterior circulation are caused by the arterial stenosis. The purposes of this study were to determine the safety of stent-assisted percutaneous transluminal angioplasty (stent-PTA) and its efficacy for the prevention of recurrent stroke in patients with symptomatic artery stenosis in the extra- and intracranial posterior circulation. Methods:, Forty-six patients with a previous stroke or TIA who received balloon-mounted coronary stents for vertebral artery origin stenosis (VAOS; 29 patients) or self-expanding nitinol stents for vertebrobasilar intracranial stenosis (VBIS; 17 patients) were followed-up for a mean of 24.1 (VAOS) and 12.7 (VBIS) months. Results:, When all cause morbidity/mortality within 30 days from stent-PTA and stroke or death from stroke in the treated vascular territory during the first 12 months of follow-up are combined, the incidence of periprocedural complications and disease progression for the first year is 10.3% in VAOS patients and 17.6% in the VBIS group. Vessel restenosis ,50% was found in 52.0% of VAOS and in 32.1% of VBIS patients who completed 6 months follow-up. Conclusions:, We observed a higher periprocedural complication rate for patients with VBIS and a higher rate of restenosis in VAOS patients after stent-PTA for symptomatic artery stenosis. [source] Synaptic and non-synaptic mechanisms of amygdala recruitment into temporolimbic epileptiform activitiesEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 10 2003Julia Klueva Abstract Lateral amygdala (LA) activity during synchronized-epileptiform discharges in temporolimbic circuits was investigated in rat horizontal slices containing the amygdala, hippocampus (Hip), perirhinal (Prh) and lateral entorhinal (LEnt) cortex, through multiple-site extra- and intracellular recording techniques and measurement of the extracellular K+ concentration. Application of 4-aminopyridine (50 µm) induced epileptiform discharges in all regions under study. Slow interictal-like burst discharges persisted in the Prh/LEnt/LA after disconnection of the Hip, seemed to originate in the Prh as shown from time delay analyses, and often preceded the onset of ictal-like activity. Disconnection of the amygdala resulted in de-synchronization of epileptiform discharges in the LA from those in the Prh/LEnt. Interictal-like activity was intracellularly reflected in LA projection neurons as ,-aminobutyric acid (GABA)A/B receptor-mediated synaptic responses, and depolarizing electrogenic events (spikelets) residing on the initial phase of the GABA response. Spikelets were considered antidromically conducted ectopic action potentials generated at axon terminals, as they were graded in amplitude, were not abolished through hyperpolarizing membrane responses (which effectively blocked evoked orthodromic action potentials), lacked a clear prepotential or synaptic potential, were not affected through blockers of gap junctions, and were blocked through remote application of tetrodotoxin at putative target areas of LA projection neurons. Remote application of a GABAB receptor antagonist facilitated spikelet generation. A transient elevation in the extracellular K+ level averaging 3 mm above baseline occurred in conjunction with interictal-like activity in all areas under study. We conclude that interictal-like discharges in the LA/LEnt/Prh spread in a predictable manner through the synaptic network with the Prh playing a leading role. The rise in extracellular K+ may provide a depolarizing mechanism for recruitment of interneurons and generation of ectopic action potentials at axon terminals of LA projection neurons. Antidromically conducted ectopic action potentials may provide a spreading mechanism of seizure activity mediated by diffuse axonal projections of LA neurons. [source] Crystal structure of the protein histidine phosphatase SixA in the multistep His-Asp phosphorelayGENES TO CELLS, Issue 1 2005Keisuke Hamada The multiple histidine-aspartate phosphorelay system plays a crucial role in cellular adaptation to environments in microorganisms and plants. Like kinase-phosphatase systems in higher eukaryotes, the multiple steps provide additional regulatory checkpoints with phosphatases. The Escherichia coli phosphatase SixA exhibits protein phosphatase activity against the histidine-containing phosphotransfer (HPt) domain located in the C-terminus of the histidine kinase ArcB engaged in anaerobic responses. We have determined the crystal structures of the free and tungstate-bound forms of SixA at 2.06 Å and 1.90 Å resolution, respectively. The results provide the first three-dimensional view of a bacterial protein histidine phosphatase, revealing a compact ,/, architecture related to a family of phosphatases containing the arginine-histidine-glycine (RHG) motif at their active sites. Compared with these RHG phosphatases, SixA lacks an extra ,-helical subdomain as a lid over the active site, thereby forming a relatively shallow groove important for the accommodation of the HPt domain of ArcB. The tungstate ion, which mimics the substrate phosphate group, is located at the centre of the active site where the active residue, His8, points to the tungsten atom in the mode of in-line nucleophilic attack. [source] High-resolution comparative genomic hybridization detects extra chromosome arm 12p material in most cases of carcinoma in situ adjacent to overt germ cell tumors, but not before the invasive tumor developmentGENES, CHROMOSOMES AND CANCER, Issue 2 2003Anne Marie Ottesen High-resolution comparative genomic hybridization (HR-CGH) analysis was performed on DNA purified from laser-capture microdissected carcinoma in situ (CIS) cells from nine cases of CIS, either from tissue without any invasive tumor or from testicular parenchyma adjacent to seminoma, nonseminoma, or a combined germ cell tumor. Before CGH analysis, DNA was amplified by degenerate oligonucleotide primed PCR (DOP-PCR) and directly labeled with a mixture of FITC-dUTP and FITC-dCTP. CGH analysis revealed extra chromosome arm 12p material in six out of seven cases with CIS adjacent to overt tumors, but only a diminutive gain of 12q was noted in one of the two cases of CIS without invasive elements. In addition, gains of parts of chromosome 8 (3/7) and losses of chromosome 5 (2/7) were demonstrated in CIS adjacent to invasive tumors. Gains of parts of chromosome 7 were found in CIS adjacent to seminoma (4/4), whereas relative gains of chromosome 15 were identified in some cases of CIS adjacent to seminoma and in isolated CIS in comparison to CIS adjacent to nonseminoma. Our data seem to indicate that extra 12p material is not present in the "dormant" CIS cell before development of an invasive tumor. The gain of extra chromosome 12 material may not be an early event in the neoplastic transformation, but is most likely associated with a more malignant progression of the CIS cell. © 2003 Wiley-Liss, Inc. [source] Beyond the regional lifeworld against the global systemworld: towards a relational ,scalar perspective on spatial,economic developmentGEOGRAFISKA ANNALER SERIES B: HUMAN GEOGRAPHY, Issue 2 2002Arnoud Lagendijk Recent writings in economic geography have questioned the way the literature has featured the regional scale in discussing issues of innovation and economic competitiveness, and called for a different conceptualization of scale. This paper takes up the challenge to go beyond what is called the ,regional gaze', by presenting a critical review of the regionalist literature and outlining an alternative approach. The critique of the ,regional gaze' is developed in two steps: first, by discussing the influence of strategic management discourse; and second, by invoking the twin concepts of lifeworld,systemworld. This critical account results in identifying various windows for elaborating an alternative conceptualization of the relationship between economic development and space. A first alternative is found in the dimension of the ,non,local' or ,extra,local', but the significance of these notions is considered to be limited. Drawing on recent work on scaling and the ,politics of scale', a relational,scalar approach is proposed that focuses on the question of how scalar qualities are socially produced and contested. What is called for is a geographical imagination that sees innovation and economic competitiveness as braced on spatialized networks rather than bounded territories scaled at the regional level. An illustration of how such a perspective may be elaborated is found in recent discussions on the concept of ,global production networks'. [source] Indirect effects of soil moisture reverse soil C sequestration responses of a spring wheat agroecosystem to elevated CO2GLOBAL CHANGE BIOLOGY, Issue 1 2010SVEN MARHAN Abstract Increased plant productivity under elevated atmospheric CO2 concentrations might increase soil carbon (C) inputs and storage, which would constitute an important negative feedback on the ongoing atmospheric CO2 rise. However, elevated CO2 often also leads to increased soil moisture, which could accelerate the decomposition of soil organic matter, thus counteracting the positive effects via C cycling. We investigated soil C sequestration responses to 5 years of elevated CO2 treatment in a temperate spring wheat agroecosystem. The application of 13C-depleted CO2 to the elevated CO2 plots enabled us to partition soil C into recently fixed C (Cnew) and pre-experimental C (Cold) by 13C/12C mass balance. Gross C inputs to soils associated with Cnew accumulation and the decomposition of Cold were then simulated using the Rothamsted C model ,RothC.' We also ran simulations with a modified RothC version that was driven directly by measured soil moisture and temperature data instead of the original water balance equation that required potential evaporation and precipitation as input. The model accurately reproduced the measured Cnew in bulk soil and microbial biomass C. Assuming equal soil moisture in both ambient and elevated CO2, simulation results indicated that elevated CO2 soils accumulated an extra ,40,50 g C m,2 relative to ambient CO2 soils over the 5 year treatment period. However, when accounting for the increased soil moisture under elevated CO2 that we observed, a faster decomposition of Cold resulted; this extra C loss under elevated CO2 resulted in a negative net effect on total soil C of ,30 g C m,2 relative to ambient conditions. The present study therefore demonstrates that positive effects of elevated CO2 on soil C due to extra soil C inputs can be more than compensated by negative effects of elevated CO2 via the hydrological cycle. [source] Caliber Fluctuations of Cervical Internal Carotid Artery and Migraine With Aura: A Possible Vasospasm Detected by Ultrasonographic ExaminationsHEADACHE, Issue 7 2009Susanna Usai MD Caliber fluctuations of extra- and intracranial arteries, mostly related to vasospasm, are often recognized in various neurological conditions. We report a case of a 33-year-old woman affected by migraine with and without aura who exhibited a possible cervical internal carotid artery vasospasm, detected by ultrasound, before a typical migraine aura. [source] Cluster Headache and Internal Carotid Artery Dissection: Two Cases and Review of the LiteratureHEADACHE, Issue 3 2008Andrea Rigamonti MD We describe 2 patients with cluster headache attacks associated with a dissection of the ipsilateral internal carotid artery at the extra,intracranial passage. These cases highlight the need for extensive neuroradiological investigation in cluster headache patients when atypical features are present. We also performed a PubMed search to review the current literature data about this association. [source] Access to health care in nursing homes: a survey in one English Health AuthorityHEALTH & SOCIAL CARE IN THE COMMUNITY, Issue 3 2000MB BCh MRCGP MPH Geraldine O'Dea Abstract The objective of the study was to establish the arrangements for provision of general practitioner (GP), nursing advice, chiropody, physiotherapy and speech and language services to nursing homes and to establish the charging policies for those services. To this end a telephone survey of the managers of the 51 nursing homes registered with one English health authority, Merton, Sutton and Wandsworth Health Authority, was undertaken. Forty-nine homes (96%) with 1541 residents responded. Twenty per cent of homes had no regular GP visits and half the homes had no planned medication reviews. One in five homes (27% of residents) had access to all health-care services. Eight homes (10% of residents) did not have access to therapy services or nursing advice. Thirty-three homes used private or both private and NHS chiropody services and 16 homes used the NHS service only. Seventeen homes used private or both private and NHS physiotherapy services with 10 homes receiving a regular private service. Twenty homes used the NHS service and 12 homes (15% of residents) had used no physiotherapy service. None used private speech and language services. Twenty-four of the 33 homes using private chiropody charged extra for this service compared with two of 10 homes using regular private physiotherapy. The findings suggest that there are inequalities in access to health care services in nursing homes. Moreover, there has been a deterioration in access to and levels of provision of NHS nursing and physiotherapy services since the national survey undertaken by the Office Population Censuses and Surveys (OPCS) in Great Britain in the mid-1980s. The new regulatory framework for older people must include systems for monitoring the provision of health services. [source] Long-term assessment of nevirapine-containing highly active antiretroviral therapy in antiretroviral-naive HIV-infected patients: 3-year follow-up of the VIRGO studyHIV MEDICINE, Issue 7 2006V Reliquet Objectives Data on the durability of antiretroviral regimens over a 3-year period have only rarely been reported. The aim of this study was to evaluate the long-term efficacy and safety of one or two daily doses of nevirapine (NVP), in combination with stavudine (d4T) and didanosine (ddI), in HIV-infected patients. Methods This study was a follow-up of the VIR (amune) Grand Ouest (VIRGO) study, a 12-month open-label trial to assess the safety and immunovirological activity of NVP-d4T-ddI combination therapy in antiretroviral-naive HIV-1-infected adults with baseline CD4 counts ,200 cells/,L and plasma viral loads ,5000 HIV-1 RNA copies/mL. Of the 100 patients included in the study, the 67 patients remaining on the initial triple therapy at the end of the study (1 year) were offered an extra 24 months of follow-up. Results Of the 60 patients who extended follow-up, 46 were still being treated with d4T-ddI-NVP at month 36; 91% (39/43) had a plasma viral load <500 copies/mL (data were missing for three patients). CD4 cell counts increased over 36 months. Safety and tolerance were good with no unexpected long-term toxicity. Conclusion After 3 years of treatment with NVP-d4T-ddI, nearly half of the patients were still receiving the initial antiretroviral therapy with a sustained and durable immunovirological benefit. Long-term toxicity was mainly related to the nucleoside reverse transcriptase inhibitor components of the regimen. [source] Surgical anatomy of the biliary tractHPB, Issue 2 2008DENIS CASTAING Abstract An intimate knowledge of the morphological, functional, and real anatomy is a prerequisite for obtaining optimal results in the complex surgery of extra and intrahepatic cholangiocarcinoma. A complete presentation of the surgical anatomy of the bile ducts includes study of the liver, hepatic surface, margins, and scissures. The frequent variations from the normal anatomy are described and an overview of the blood supply and lymphatics of the biliary tract is presented. [source] Delivery of Nucleic Acids via Disulfide-Based Carrier SystemsADVANCED MATERIALS, Issue 32-33 2009Sonja Bauhuber Abstract Nucleic acids are not only expected to assume a pivotal position as "drugs" in the treatment of genetic and acquired diseases, but could also act as molecular cues to control the microenvironment during tissue regeneration. Despite this promise, the efficient delivery of nucleic acids to their side of action is still the major hurdle. One among many prerequisites for a successful carrier system for nucleic acids is high stability in the extracellular environment, accompanied by an efficient release of the cargo in the intracellular compartment. A promising strategy to create such an interactive delivery system is to exploit the redox gradient between the extra- and intracellular compartments. In this review, emphasis is placed on the biological rationale for the synthesis of redox sensitive, disulfide-based carrier systems, as well as the extra- and intracellular processing of macromolecules containing disulfide bonds. Moreover, the basic synthetic approaches for introducing disulfide bonds into carrier molecules, together with examples that demonstrate the benefit of disulfides at the individual stages of nucleic acid delivery, will be presented. [source] DXA scanning in women over 50 years with distal forearm fracture shows osteoporosis is infrequent until age 65 yearsINTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Issue 3 2008H. Lashin Summary Aims:, Women with distal forearm fracture (DFF) may have low bone mineral density (BMD) and merit Dual Energy Xray (DXA) scanning. However patient age at fracture and the database for ,healthy' subjects may influence how many have osteoporosis and require DXA scans. Osteoporosis prevalence in DFF patients by age was investigated using local or nHanes III databases for BMD. Methods:, A total of 186 women over 50 years consecutively referred with DFF over 1 year were audited without exclusion criteria. BMD of L2,4 and femoral neck (Hologic QDR4500A) was measured and T - and Z -scores calculated from a local database or nHanes III. Results:, Of 90 patients aged 50,64 years, 21.1% had femoral neck T -score < ,2.5 and 7.7% < ,3.0 (local) and 8.8% and 4.4% respectively (nHanes III). Patients aged 65,74 years (n = 61) included 19.7% with T -score < ,2.5 (nHanes III = 10%). 41.2% (nHanes III = 28.6%) of patients > 75 years had femoral neck osteoporosis. Including patients with spine T < ,2.5 increased the proportion to 31.1% (50,64 years) and 34.4% (65,74 years) with no extra over 75 years. Weight predicted low BMD ineffectively (area under ROC = 70%). Conclusion:, Osteoporosis is infrequent in women with DFF below 65 years. As fracture prevention treatment yields significant fracture reduction only in patients with T -score < ,2.5, DXA scanning below 65 years is not justified. After 65 years scanning is justified at all ages, as even in the elderly patients osteoporosis is present in < 50% of patients with DFF. Using nHanes III limits the number of DFF patients warranting treatment. Low body weight is unreliable for identifying osteoporosis. [source] Evaluating the expedited forwarding of voice traffic in a differentiated services networkINTERNATIONAL JOURNAL OF COMMUNICATION SYSTEMS, Issue 9 2002Artur Ziviani Abstract The Differentiated Services architecture offers a scalable alternative to provide Quality of Service (QoS) to the new multimedia applications in the Internet. This paper aims at evaluating the delay and jitter experienced by voice traffic when handled by the Expedited Forwarding (EF) scheme. The analysis includes the effects of different packet scheduling mechanisms implementing EF and of the voice packet size. We also evaluate how efficiently each type of traffic uses an extra allocated bandwidth and the impact of traffic shaping. The results show that increasing the service rate share allocated to the EF aggregate does not significantly affect the competing best effort (BE) traffic. This holds as long as the BE traffic can use the bandwidth left unused by the EF traffic in idle periods. Copyright © 2002 John Wiley & Sons, Ltd. [source] Divine Action and the Trinity: A Brief Exploration of the Grounds of Trinitarian Speech about God in the Theology of Adolf SchlatterINTERNATIONAL JOURNAL OF SYSTEMATIC THEOLOGY, Issue 3 2002Andreas Loos This article explores Schlatter's doctrine of the Trinity in the light of the contemporary debate, focusing on the relation of the economic and the ontological Trinity. It is shown that Schlatter relates God's triune being and God's trinitarian action through the notion of love , where God's love ad extra as well as ad intra is oriented toward the particular in such a way as to enable true otherness. It will be argued, moreover, that Schlatter's contribution to the contemporary trinitarian debate lies in propounding an applied trinitarian theology which is faithful to its object. When God in himself and in relation to creation is oriented toward and actively seeks the other, then theology cannot talk about God's being apart from the actuality of his actions in this world. [source] ,Instrument of the union of hearts': The Theology of Personhood and the BishopINTERNATIONAL JOURNAL OF SYSTEMATIC THEOLOGY, Issue 3 2002A. N. Williams This article explores Schlatter's doctrine of the Trinity in the light of the contemporary debate, focusing on the relation of the economic and the ontological Trinity. It is shown that Schlatter relates God's triune being and God's trinitarian action through the notion of love , where God's love ad extra as well as ad intra is oriented toward the particular in such a way as to enable true otherness. It will be argued, moreover, that Schlatter's contribution to the contemporary trinitarian debate lies in propounding an applied trinitarian theology which is faithful to its object. When God in himself and in relation to creation is oriented toward and actively seeks the other, then theology cannot talk about God's being apart from the actuality of his actions in this world. [source] Catholic, Calvinist, and Lutheran Doctrines of Eucharistic Presence: A Brief Note towards a RapprochementINTERNATIONAL JOURNAL OF SYSTEMATIC THEOLOGY, Issue 3 2002Richard Cross This article explores Schlatter's doctrine of the Trinity in the light of the contemporary debate, focusing on the relation of the economic and the ontological Trinity. It is shown that Schlatter relates God's triune being and God's trinitarian action through the notion of love , where God's love ad extra as well as ad intra is oriented toward the particular in such a way as to enable true otherness. It will be argued, moreover, that Schlatter's contribution to the contemporary trinitarian debate lies in propounding an applied trinitarian theology which is faithful to its object. When God in himself and in relation to creation is oriented toward and actively seeks the other, then theology cannot talk about God's being apart from the actuality of his actions in this world. [source] Are Free Trade Areas Good for Multilateralism?INTERNATIONAL STUDIES QUARTERLY, Issue 4 2002Evidence from the European Free Trade Association Do free trade agreements (FTAs) help or hinder multilateral trade liberalization? This question, though much debated, remains unanswered because (1) there has been scant attention to the conditions under which FTAs have either effect, and (2) extant hypotheses have not been rigorously tested. In this article I identify conditions under which FTAs help and hinder broader trade liberalization: they do the former when members' intra, and extra,FTA comparative advantages are similar and the latter when the opposite is true. I test these hypotheses using trade, output, and tariff data from the European Free Trade Association. The trade data indicate that members with similar intra, and extra,FTA comparative advantages liberalized trade more rapidly than those with dissimilar comparative advantages. The output and tariff data suggest that these differences among members reflect hypothesized economic and political processes. My research implies that scholars should abandon universalistic arguments concerning the effects of regional arrangements and devote more attention to the conditions governing the relationship between regionalism and multilateralism. [source] The Energetics of Ion Distribution: The Origin of the Resting Electric Potential of CellsIUBMB LIFE, Issue 5 2002Richard L. Veech Abstract The relation between the energies of ion movement and ATP hydrolysis is unknown in tissues with widely varying electric potentials. Consequently, we measured the concentration of the nine major inorganic ions in the extra- and intracellular phases in heart, liver, and red cells with resting electrical potentials, E N, of -86, -28, and -6 mV, respectively, under six different physiological conditions. We calculated the Nernst electric potential and the energy of ion movement between the phases. We found that the energy of ATP hydrolysis was essentially constant, between -54 and -58 kJ/mol, in all tissues and conditions. In contrast, as E N decreased, the energies of the Na + and K + gradients decreased, with slopes approximating their valence. The difference between the energies of Na + and K + gradients remained constant at 17 kJ/mol, which is approximately one third of the energy of ATP hydrolysis, demonstrating near-equilibrium of the Na +/K + ATPase in all tissues under all conditions. All cations, except K +, were pumped out of cells and all anions, except Cl - in liver and red cell, were pumped into cells. We conclude that the energy of ATP was expressed in Na +/K + ATPase and its linked inorganic ion transporters to create a Gibbs-Donnan near-equilibrium system, an inherent part of which was the electric potential. [source] Theorizing Religious Effects Among American AdolescentsJOURNAL FOR THE SCIENTIFIC STUDY OF RELIGION, Issue 1 2003Christian Smith A large body of empirical studies shows that religion often serves as a factor promoting positive, healthy outcomes in the lives of American adolescents. Yet existing theoretical explanations for these religious effects remain largely disjointed and fragmented. This article attempts to formulate a more systematic, integrated, and coherent account of religion's constructive influence in the lives of American youth, suggesting nine key factors (moral directives, spiritual experiences, role models, community and leadership skills, coping skills, cultural capital, social capital, network closure, and extra,community links) that cluster around three key dimensions of influence (moral order, learned competencies, and social and organizational ties). [source] Interconversion of intra- and extra-chromosomal sites of gene amplification by modulation of gene expression and DNA methylationJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2007Noriaki Shimizu Abstract We previously showed that plasmids containing a mammalian replication initiation region and a matrix attachment region were efficiently amplified to few thousand copies per cell, and that they formed extrachromosomal double minutes (DMs) or chromosomal homogeneously staining regions (HSRs). In these structures, the plasmid sequence was arranged as a tandem repeats, and we suggested a mechanism of plasmid amplification. Since amplification was very efficient, easy, and convenient, it might be adapted to a novel method for protein production. In the current study, we found that gene expression from the tandem plasmid repeat was suppressed. We identified several strategies to overcome this suppression, including: (1) use of higher concentrations of antibiotic during cell selection; (2) treatment of cells with agents that influence DNA methylation (5-azacytidine) or histone acetylation (butyrate); (3) co-amplification of an insulator sequence; and (4) co-amplification of sequences that encode a transcriptional activator. Expression from the plasmid repeat was always higher at DMs compared to HSRs. We found that continuous activation of a plasmid-encoded inducible promoter prevented the generation of long HSRs, and favored amplification at DMs. Consistent with this finding, there was a synergistic effect of transcriptional activation and inhibition of DNA methylation on the fragmentation of long HSRs and the generation of DMs and short HSRs. Our results indicate that both transcriptional activation and DNA methylation regulate the interconversion between extra- and intra-chromosomal gene amplification. These results have important implications for both protein production technology, and the generation of chromosomal abnormalities found in human cancer cells. J. Cell. Biochem. 102: 515,529, 2007. © 2007 Wiley-Liss, Inc. [source] Paradoxical roles for lysyl oxidases in cancer,A prospectJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 6 2007Stacey L. Payne Abstract Lysyl oxidase (LOX) is an extracellular matrix (ECM) enzyme that catalyzes the cross-linking of collagens or elastin in the extracellular compartment, thereby regulating the tensile strength of tissues. However, recent reports have demonstrated novel roles for LOX, including the ability to regulate gene transcription, motility/migration, and cell adhesion. These diverse functions have led researchers to hypothesize that LOX may have multiple roles affecting both extra- and intracellular cell function(s). Particularly noteworthy is aberrant LOX expression and activity that have been observed in various cancerous tissues and neoplastic cell lines. Both down and upregulation of LOX in tumor tissues and cancer cell lines have been described, suggesting a dual role for LOX as a tumor suppressor, as well as a metastasis promoter gene,creating a conundrum within the LOX research field. Here, we review the body of evidence on LOX gene expression, regulation, and function(s) in various cancer cell types and tissues, as well as stromal,tumor cell interactions. Lastly, we will examine putative mechanisms in which LOX facilitates breast cancer invasion and metastasis. Taken together, the literature demonstrates the increasingly important role(s) that LOX may play in regulating tumor progression and the necessity to elucidate its myriad mechanisms of action in order to identify potentially novel therapeutics. J. Cell. Biochem. 101: 1338,1354, 2007. © 2007 Wiley-Liss, Inc. [source] | ||||||||||||||||||||||||||||||||||||||||||||||